Nanoconfined Silver Nanoclusters Combined with X-Shaped DNA Recognizer-Triggered Cascade Amplification for Electrochemiluminescence Detection of APE1

Analytical Chemistry, Год журнала: 2024, Номер 96(28), С. 11383 - 11389

Опубликована: Июль 1, 2024

Apurinic/apyrimidinic endonuclease 1 (APE1), as a vital base excision repair enzyme, is essential for maintaining genomic integrity and stability, its abnormal expression closely associated with malignant tumors. Herein, we constructed an electrochemiluminescence (ECL) biosensor detecting APE1 activity by combining nanoconfined ECL silver nanoclusters (Ag NCs) X-shaped DNA recognizer-triggered cascade amplification. Specifically, the Ag NCs were prepared confined in glutaraldehyde-cross-linked chitosan hydrogel network using one-pot method, resulting strong response exceptional stability comparison discrete NCs. Furthermore, self-assembled recognizers designed detection, which not only improved reaction kinetics due to ordered arrangement of recognition sites but also achieved high sensitivity utilizing amplification strand displacement (SDA) DNAzyme catalysis. As expected, this sensitive detection range 1.0 × 10–3 U·μL–1 10–10 limit 2.21 10–11 U·μL–1, rendering it desirable approach biomarker detection.

Язык: Английский

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MiRNA and mRNA-Controlled Double-Cascaded Amplifying Circuit Nanosensor for Accurate Discrimination of Breast Cancers in Living Cells, Animals, and Organoids

Analytical Chemistry, Год журнала: 2024, Номер 96(10), С. 4154 - 4162

Опубликована: Март 1, 2024

Metastasis is the leading cause of death in patients with breast cancer. Detecting high-risk cancer, including micrometastasis, at an early stage vital for customizing right and efficient therapies. In this study, we propose enzyme-free isothermal cascade amplification-based DNA logic circuit situ biomineralization nanosensor, HDNAzyme@ZIF-8, simultaneous imaging multidimensional biomarkers live cells. Taking miR-21 Ki-67 mRNA as dual detection targets achieved sensitive operations molecular recognition through hybridization chain reaction DNAzyme. The HDNAzyme@ZIF-8 nanosensor has ability to accurately differentiate cancer cells their subtypes by comparing relative fluorescence intensities. Of note, our can also achieve visualization within organoids, faithfully recapitulating functional characteristics parental tumor. Overall, combination these techniques offers a universal strategy detecting cancers high sensitivity holds vast potential clinical diagnosis.

Язык: Английский

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Novel samarium-based metal-organic frameworks with antenna effect-induced electrochemiluminescence for acetamiprid assay

Sensors and Actuators B Chemical, Год журнала: 2025, Номер 430, С. 137343 - 137343

Опубликована: Янв. 29, 2025

Язык: Английский

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NAPTUNE: nucleic acids and protein biomarkers testing via ultra-sensitive nucleases escalation

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 4, 2025

Язык: Английский

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Isotope Dilution DNA Logic Circuits for Multiple Output and Absolute Quantification

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 6, 2025

DNA logic circuits have gained great success in the past, thanks to their distinct performance regarding scalability and correctness of computation. However, there are still two challenges often considered for circuit-based First, mainstream optical probes subject spectral overlapping interference complex multitask analysis outputs. Second, absolute quantification results traceable primary international system units mission impossible, especially interlaboratory comparisons quality assurances. Herein, we constructed encoded with lanthanide isotopes decoded by elemental mass spectrometry. The 155Gd-enriched isotope 145Nd-enriched were incorporated dilution-based microRNAs. proposed isotopic greatly enhance multiplexity computation accuracy, which poses a potential cancer biomarker-related diagnosis.

Язык: Английский

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Label-free colorimetric detection platform based on catalytic hairpin self-assembly and G-quadruplex/hemin DNAzyme for comprehensive biomarker profiling

Talanta, Год журнала: 2024, Номер 272, С. 125835 - 125835

Опубликована: Фев. 25, 2024

Язык: Английский

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An ultrasensitive electrochemical biosensor based on logic transcription circuit-activated DNAzyme amplifier for flap endonuclease 1 activity detection

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 415, С. 135986 - 135986

Опубликована: Май 21, 2024

Язык: Английский

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On‐Site Multiply Stimulated Self‐Confinement of an Integrated DNA Cascade Circuit for Highly Reliable Intracellular Imaging of miRNA and In Situ Interrogation of the Relevant Regulatory Pathway

Small, Год журнала: 2024, Номер unknown

Опубликована: Сен. 16, 2024

Abstract Artificial DNA circuits represent a versatile yet promising toolbox for in situ monitoring and concomitant regulation of diverse biological events within live cells. Nonetheless, their performance is significantly impeded by the diffusion‐dominated slow reaction kinetics uncontrollable off‐target activation. Herein, self‐localized cascade (SLC) circuit reported robust efficient microRNA (miRNA) analysis living The SLC consists cell‐specific localization module analyte‐specific signal amplification module. By integrating probes these two modules, complexity system reduced to realize responsive co‐localization circuitry simultaneous amplification. Taking advantage specifically activated, self‐localized, design, successfully achieves miRNA‐21 (miR‐21) imaging accurate cells differentiation. Moreover, reverse mechanism explored between messenger RNA (mRNA) miRNA through engineered further elucidates underlying signaling pathways them. Therefore, provides powerful tool sensitive detection intracellular biomolecules study corresponding cell regulatory mechanisms.

Язык: Английский

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Highly-sensitive and logic platform based on spatially-constrained T7 transcription enhanced Cas13a for DNA repair enzyme detection and intracellular imaging

Biosensors and Bioelectronics, Год журнала: 2025, Номер unknown, С. 117406 - 117406

Опубликована: Март 1, 2025

Язык: Английский

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Fluorogenic Aptamer-Based Hybridization Chain Reaction for Signal-Amplified Imaging of Apurinic/Apyrimidinic Endonuclease 1 in Living Cells

Biosensors, Год журнала: 2024, Номер 14(6), С. 274 - 274

Опубликована: Май 27, 2024

A fluorogenic aptamer (FA)-based hybridization chain reaction (HCR) could provide a sensitive and label-free signal amplification method for imaging molecules in living cells. However, existing FA-HCR methods usually face some problems, such as complicated design significant background leakage, which greatly limit their application. Herein, we developed an FA-centered HCR (FAC-HCR) based on remote toehold-mediated strand displacement reaction. Compared to traditional HCRs mediated by four hairpin probes (HPs) two HPs, the FAC-HCR displayed significantly decreased leakage improved sensitivity. Furthermore, was used test non-nucleic acid target, apurinic/apyrimidinic endonuclease 1 (APE1), important BER-involved endonuclease. The fluorescence analysis results confirmed that can reach detection of 0.1174 U/mL. By using HPs with polyetherimide-based nanoparticles, activity APE1 cells be imaged. In summary, this study new idea FA-based improve performance live cell imaging.

Язык: Английский

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