ACS Applied Bio Materials, Год журнала: 2024, Номер unknown
Опубликована: Дек. 11, 2024
Multielemental transition metal compounds represent a class of promising candidates for the biomedical field due to their unique structure and application potential. However, synthesis process remains challenging, which was subject high-temperature treatment multimetallic elements integrated within one system. Herein, first time, we have fabricated nanotripod,
Язык: Английский
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Апрель 3, 2025
Disulfidptosis and ferroptosis are recently identified programmed cell deaths for tumor therapy, both of which highly depend on the intracellular cystine/cysteine transformation cystine transporter solute carrier family 7 member 11/glutathione/glutathione peroxidase 4 (SLC7A11/GSH/GPX4) antioxidant axis. However, disulfidptosis usually asynchronous due to opposite effect transport them. Herein, systematic glucose deprivation, by inhibiting upstream uptake promoting downstream consumption, is proposed synchronously evoke ferroptosis. As an example, Au nanodots Fe-apigenin (Ap) complexes coloaded FeOOH nanoshuttles (FeOOH@Fe-Ap@Au NSs) employed regulate SLC7A11/GSH/GPX4 axis performing disulfidptosis- ferroptosis-mediated therapy synchronously. In this scenario, exhibit oxidase-like activity when consuming massive glucose. Meanwhile, Ap can inhibit downregulating 1, depriving fundamentally. The systematical deprivation limits supplement NADPH suppresses axis, thus solving contradiction addition, efficient delivery exogenous iron ions FeOOH@Fe-Ap@Au NSs self-supplied H2O2 through nanodots-catalytic oxidation facilitate Fenton reaction therewith help amplify a result synchronous occurrence ferroptosis, good efficacy in ovarian cancer therapeutic model.
Язык: Английский
Процитировано
2
Advanced Healthcare Materials, Год журнала: 2025, Номер unknown
Опубликована: Апрель 21, 2025
Abstract The peroxynitrite anion (ONOO − ) is the product of a specific reaction between nitric oxide (NO) and superoxide (O 2 •− ), showing great potential for treating multidrug‐resistant bacterial infections. However, antibacterial materials with highly efficient ONOO controlled release properties are lacking. In this study, hollow copper single atom nanozyme (Cu HSAz) oxidase (OXD)‐mimicking property rationally designed used first time as effective delivery carrier NO donor N, N ′‐di‐sec‐butyl‐ ′‐dinitroso‐1, 4‐phenylenediamine (BNN6), obtaining Cu HSAz@BNN6. Upon near infrared (NIR)‐II laser irradiation, HSAz@BNN6 simultaneously releases substantial amounts O , which subsequently lead to boosting via cascade reaction. Remarkably, displays excellent efficiency by considerably inhibiting activity methicillin‐resistant Staphylococcus aureus ( MRSA activity. Moreover, in ‐associated wound infections promoting healing. An mechanism study reveals that triggers death firstly damaging membrane, followed destructing metabolism cut adenosine triphosphate energy supply boosts intracellular reactive oxygen species generation, depletes glutathione caused DNA breakage.
Язык: Английский
Процитировано
1
Journal of Colloid and Interface Science, Год журнала: 2025, Номер 693, С. 137611 - 137611
Опубликована: Апрель 15, 2025
Язык: Английский
Процитировано
0
Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Май 16, 2025
In colorectal cancer treatment, chemotherapeutic agents induce reactive oxygen species (ROS) production, which promotes NAD+ accumulation in tumor cells, reducing treatment sensitivity and worsening patient prognosis. Targeted depletion of presents a promising strategy to overcome resistance improve Here, we designed dual-metallic nanozyme (CuMnOx-V@Oxa@SP) with defect engineering, modified by soy phospholipids (SP) loaded oxaliplatin (Oxa). This uses its oxygen-deficient active sites rapidly irreversibly degrade NAD⁺ NADH into nicotinamide ADP-ribose derivatives, disrupting the electron transport chain (ETC) compromising antioxidant defenses. It also inhibits glutathione S-transferase P1 (GSTP1) pathway, weakening detoxification enhancing chemotherapy sensitivity. Density functional theory calculations revealed that synergistic effect among multi-enzyme centers endows CuMnOx-V nanozymes excellent catalytic activity. microenvironment (TME), exhibit peroxidase, oxidase, oxidase-mimicking activities. generates multiple ROS depletes while preventing their regeneration thereby triggering cascade amplification oxidative stress. This, coupled targeted drug delivery, restores chemosensitivity refractory tumors exposes vulnerabilities resistant cells ROS.
Язык: Английский
Процитировано
0
Talanta, Год журнала: 2025, Номер 296, С. 128398 - 128398
Опубликована: Май 28, 2025
Язык: Английский
Процитировано
0
Advanced Functional Materials, Год журнала: 2025, Номер unknown
Опубликована: Май 30, 2025
Abstract Sonodynamic therapy (SDT) exhibits high tissue penetration and negligible radiation damage to normal tissue, but it hampered by the limited oxygen in tumor, which can be potentiated via improving metabolism increasing utilization efficiency. Herein, a multifunctional biomimetic nanocarrier (VI@R‐T) is fabricated encapsulating vanadium‐based nanozyme (VO x ) with dual enzyme activity sonosensitizer indocyanine green (ICG) into mitochondria‐targeted erythrocyte vesicle. The targeted mitochondria, where red blood cell membrane catalyzed endogenous H 2 O increase content for alleviating hypoxia. Meanwhile, NADH oxidase (NOX) affecting nicotinamide adenine dinucleotide/flavin dinucleotide (NADH/FAD) balance cellular oxidative phosphorylation, impairs mitochondrial electron transport chain reduces intracellular consumption, thereby significantly tumor efficacy of SDT. In addition, also shows peroxide‐like microenvironment (TME), generates highly toxichydroxyl radical (OH) ROS‐amplifying stress. Deep‐hypothermia 2D/3D autofluorescence imaging monitored effectively disrupt NADH/FAD tumor. This work provides anew approach designing ROS‐based nanomedical platform hypoxia relief.
Язык: Английский
Процитировано
0
Colloids and Surfaces B Biointerfaces, Год журнала: 2025, Номер 254, С. 114844 - 114844
Опубликована: Июнь 3, 2025
Язык: Английский
Процитировано
0
Biomaterials, Год журнала: 2025, Номер 324, С. 123465 - 123465
Опубликована: Июнь 3, 2025
Язык: Английский
Процитировано
0
Cell Communication and Signaling, Год журнала: 2024, Номер 22(1)
Опубликована: Дек. 18, 2024
The innate immune system serves as the host's first line of defense against invading pathogens. Stimulator interferon genes (STING) is a key component this system, yet its relationship with glucose metabolism, particularly in antiviral immunity, remains underexplored. Metabolomics analysis was used for detecting metabolic alterations spleens from STING knockout (KO) and wild-type (WT) mice. Co-immunoprecipitation employed determining ubiquitination TKT. Mass spectrometry interaction proteins STING. Enzyme activity kits were activities TKT G6PD. In study, we demonstrate that herpes simplex virus (HSV) infection activates pentose phosphate pathway (PPP) host cells, thereby initiating an response. Using STING-manipulated cells systemic mice, show positively regulates PPP, which, turn, limits HSV infection. Inhibition PPP significantly reduced production factors dampened STING-induced responses. Mechanistically, discovered interacts transketolase (TKT), enzyme non-oxidative branch reduces via E3 ubiquitin ligase UBE3A, stabilizing Silencing or inhibiting oxythiamine diminished factor production. Our findings reveal plays synergistic role generating during viral suggest activation could serve adjunct strategy therapy.
Язык: Английский
Процитировано
2
Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2024, Номер 320, С. 124565 - 124565
Опубликована: Май 31, 2024
Язык: Английский
Процитировано
1