Analytical Chemistry,
Год журнала:
2024,
Номер
97(1), С. 602 - 612
Опубликована: Дек. 23, 2024
Nowadays,
optical
tweezers
play
a
vital
role
not
only
in
manipulation
but
also
bioassay.
As
principal
trapping
objects,
microbeads
can
combine
with
suspension
array
technology,
amply
focused
laser
beams
and
adequately
concentrated
tags
contributing
to
highly
sensitive
detection.
In
view
of
the
inefficiency
conventional
single-trap
tweezers,
multitrap
systems
are
developed.
Here,
green-
blue-emitting
core-shell-shell
upconversion
nanoparticles
(UCNPs)
adopted
encode
determine
dual
miRNAs,
internal
shells
leading
luminescence
process
facilitate
quenching
through
resonance
energy
transfer
(LRET).
Utilizing
trans
cleavage
CRISPR/Cas12a,
quenched
signals
recovered
amplified,
causing
further
enhanced
detection
sensitivity.
Ultimately,
limits
(LOD)
17
22
aM
obtained
excellent
specificities
verified.
Furthermore,
miRNAs
from
MCF-7,
A549,
MCF-10A
cells
extracted
detected,
results
consistent
those
by
PCR.
Notably,
miR-155
MCF-7
A549
is
detectable
at
single-cell
level.
Thus,
differences
measured
miRNA
levels
between
imply
potential
this
method
discriminate
breast
cancer
epithelial
despite
difficulty
distinguishing
different
due
similar
levels.
Chemosensors,
Год журнала:
2025,
Номер
13(2), С. 60 - 60
Опубликована: Фев. 8, 2025
Pathogens
and
their
metabolites
in
food
present
significant
risks
to
both
human
health
economic
development.
Rising
living
standards
increasing
awareness
of
safety
have
driven
the
demand
for
sensitive
rapid
detection
methods.
Lanthanide-doped
upconversion
nanoparticles
(UCNPs),
with
exceptional
optical
properties,
emerged
as
a
promising
platform
developing
biosensors
detect
pathogenic
bacteria
metabolites.
The
integration
UCNPs
point-of-care
testing
(POCT)
has
garnered
considerable
attention
its
portability
immediacy,
highlighting
future
biosensing,
particularly
applications
requiring
quick
accurate
diagnostics.
This
review
explores
recognition
elements
design
principles
commonly
used
UCNP-based
examines
various
applications,
including
lateral
flow
assays,
microfluidic
systems,
photoelectrochemical
devices,
smartphone-integrated
platforms.
Despite
advancements,
challenges
remain
applicability
commercialization
biosensing
technology.
Future
research
should
focus
on
enhancing
sensitivity
specificity,
scalable
cost-effective
production
methods,
integrating
advanced
digital
technologies
enable
broader
adoption.
Addressing
these
challenges,
establishing
regulatory
frameworks,
considering
sustainability
will
be
crucial
fully
realizing
potential
biosensors.
Chemical Reviews,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 17, 2025
Lanthanides
are
particularly
effective
in
their
clinical
applications
magnetic
resonance
imaging
and
diagnostic
assays.
They
have
open-shell
4f
electrons
that
give
rise
to
characteristic
narrow,
line-like
emission
which
is
unique
from
other
fluorescent
probes
biological
systems.
Lanthanide
luminescence
signal
offers
selection
of
detection
pathways
based
on
the
choice
ion
visible
near-infrared
with
long
lifetimes
lend
themselves
time-resolved
measurements
for
optical
multiplexing
schemes
novel
bioimaging
applications.
The
delivery
lanthanide
agents
cells
allows
localized
bioresponsive
activity
therapies.
Detection
region
spectrum
coupled
technological
advances
microscopies
opens
new
avenues
deep-tissue
surgical
interventions.
This
review
focuses
different
ways
can
be
exploited
nucleic
acid
enzyme
detection,
anion
recognition,
cellular
imaging,
tissue
photoinduced
therapeutic
We
focused
hierarchy
designs
include
luminescent
lanthanides
as
biology
considering
coordination
complexes,
multimetallic
systems
metal-organic
frameworks
nanoparticles
highlighting
strategies
downshifting,
upconversion
revealing
some
opportunities
challenges
offer
potential
further
development
field.
Cell Biology and Toxicology,
Год журнала:
2024,
Номер
40(1)
Опубликована: Ноя. 20, 2024
Gastric
cancer
(GC)
is
the
fifth
most
common
worldwide,
particularly
prevalent
in
Asia,
especially
China,
where
both
its
incidence
and
mortality
rates
are
significantly
high.
Meanwhile,
nanotechnology
has
demonstrated
great
potential
treatment
of
GC.
In
particular,
nanodrug
delivery
systems
have
improved
therapeutic
efficacy
targeting
through
various
functional
modifications,
such
as
peptides,
tumor
microenvironment
responsiveness,
instrument-based
methods.
For
instance,
silica
(SiO2)
excellent
biocompatibility
can
be
used
a
drug
carrier,
with
porous
structure
enhancing
loading
capacity.
Polymer
nanoparticles
regulate
release
mechanisms
by
altering
material
composition
preparation
Lipid
efficiently
encapsulate
hydrophilic
drugs
promote
cellular
uptake,
while
carbon-based
biosensors
delivery.
Targets
integrins,
HER2
receptors,
been
to
improve
GC
treatment.
Nanodrug
techniques
not
only
enhance
capabilities
but
also
selectively
target
cells.
Currently,
there
lack
systematic
summarization
synthesis
regarding
relationship
between
treatment,
which
some
extent
hinders
researchers
clinicians
from
searching
for
referencing
related
studies,
thereby
reducing
work
efficiency.
This
study
aims
systematically
summarize
existing
research
on
making
it
easier
professionals
search
reference,
promoting
further
role
their
clinical
applications
review
discusses
functionalized
nanocarriers
recent
years,
including
surface
modifications
targeted
markers,
combination
phototherapy,
chemotherapy,
immunotherapy,
along
advantages
challenges.
It
examines
future
prospects
nanomaterials
The
focuses
combined
application
multiple
modalities,
demonstrating
multimodal
treatments.
Furthermore,
thoroughly
explores
specific
challenges
that
face
biocompatibility,
control,
translation
issues,
providing
outlook
developments.
Additionally,
this
emphasizes
value
feasibility
applications,
contrasting
reviews
focus
basic
research.
Through
these
innovations,
we
offer
new
perspectives
directions
development
Advanced Optical Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 12, 2025
Abstract
Upconversion
emissions
from
lanthanide
ions
have
unparalleled
advantages
in
the
field
of
temperature
sensing
and
information
encryption.
Despite
extensive
research
on
probes,
developing
highly
sensitive
measurement
applications
still
poses
a
significant
challenge.
This
study
utilizes
doped
BaTiO₃
as
foundational
material
to
develop
fiber
optic
probe
with
enhanced
sensitivity.
The
optical
temperature‐sensing
capabilities
are
assessed
based
intensity
ratio
various
energy
levels
BaTiO₃:
Yb/Tm/Er
phosphors.
Notably,
non‐thermal
coupling
level
between
Tm
Er
demonstrates
highest
sensitivity,
achieving
maximum
relative
sensitivity
2.70%
K⁻¹
at
303
K.
By
leveraging
temperature‐dependent
color
change
material,
large‐capacity
photonic
barcode
for
storage
encrypted
transmission
is
developed,
thereby
expanding
potential
monitoring.
ABSTRACT
Extracellular
vesicles
(EVs)
are
essential
for
host–pathogen
interactions,
mediating
processes
such
as
immune
modulation
and
pathogen
survival.
Pathogen‐derived
EVs
hold
significant
diagnostic
potential
because
of
their
unique
cargo,
offering
a
wealth
biomarkers.
In
this
review,
we
first
discuss
the
roles
derived
from
various
pathogens
in
interactions
summarize
latest
advancements
detection
based
on
EVs.
Then,
highlight
innovative
strategies,
including
novel
aggregate
materials
machine
learning
approaches,
enhancing
EV
analysis.
Finally,
challenges
field
future
directions
advancing
EV‐based
diagnostics,
aiming
to
translate
these
insights
into
clinical
applications.
Nanomaterials-integrated
CRISPR/Cas
systems
have
rapidly
emerged
as
powerful
next-generation
platforms
for
optical
biosensing.
These
integrated
harness
the
precision
of
CRISPR/Cas-mediated
nucleic
acid
detection
while
leveraging
unique
properties
nanomaterials
to
achieve
enhanced
sensitivity
and
expanded
analytical
capabilities,
thereby
broadening
their
diagnostic
potential.
By
incorporating
a
diverse
range
nanomaterials,
these
effectively
expand
toolbox
detection,
offering
adaptable
solutions
tailored
various
challenges.
This
review
provides
comprehensive
overview
successfully
into
CRISPR/Cas-based
sensing
systems.
It
examines
multiple
modalities,
including
fluorescence,
electrochemiluminescence,
colorimetry,
surface-enhanced
Raman
spectroscopy,
highlighting
how
facilitate
signal
amplification,
enable
multiplexing,
support
development
point-of-care
applications.
Additionally,
practical
applications
in
critical
fields
such
healthcare
diagnostics
environmental
monitoring
are
showcased.
While
offer
considerable
advantages,
several
real-world
challenges
complexity
assay
workflows,
impact
cost,
regulatory
hurdles
must
be
addressed
before
widespread
implementation
can
achieved.
identifying
obstacles
proposing
strategic
solutions,
we
aim
pave
way
continued
advancement
adoption
nanomaterial-integrated
biosensing
technologies.
