High-throughput Generation of Collagen Microbeads for 3D Cell Culture and Extracellular Vesicle Production DOI Creative Commons
Samantha Ali,

Fabiana Mastantuono,

Andrea Orozco-Torres

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 3, 2024

ABSTRACT Collagen type I, a fundamental component of natural extracellular matrices across species, is an attractive material for the development tissue engineering constructs. Nevertheless, poor mechanical properties and thermal instability hinder its use specific construction 3D cultures printing. In this study, we present microfluidic high-throughput approach producing high-quality uniform collagen microbeads without introducing any chemical modification. We achieved rapid droplet fabrication with sizes spanning from 50 µm to 1200 in production rate up 10000 droplets per minute. The resulting can serve as numerous microbioreactors which are suspended culture medium precipitation ideal cell growth. demonstrated excellent compatibility, facilitating attachment proliferation, well promoting vesicle secretion microbeads. This technology facile versatile high throughput culture, heterogeneous modeling, production, essential drug delivery screening.

Язык: Английский

Real-time quantification of microfluidic hydrogel crosslinking via gas-phase electrophoresis DOI
Po-Yen Lai, Duraisamy Senthil Raja, Je‐Wei Chang

и другие.

Journal of Colloid and Interface Science, Год журнала: 2025, Номер 684, С. 201 - 212

Опубликована: Янв. 7, 2025

Язык: Английский

Процитировано

0

A bibliometric analysis of hydrogel research in various fields: the trends and evolution of hydrogel application DOI Creative Commons

Zhong-Zhu Yuan,

Fan Yang,

Sheng Cheng

и другие.

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Янв. 31, 2025

Hydrogel, a polymer material with three-dimensional structure, has considerably expanded in research across multiple fields lately. However, the lack of comprehensive review integrating status hydrogel diverse hindered development hydrogel. This bibliometric analysis reviewed hydrogel-related over past decades, emphasizing evolution, status, and future directions within multitude fields, such as materials science, chemistry, engineering, physics, biochemistry molecular biology, pharmacology pharmacy, cell biotechnology applied microbiology, etc. We encapsulated applications potential wound healing, drug delivery, encapsulation, bioprinting, tissue electronic products, environment applications, disease treatment. study integrated current matrix system characteristics hydrogels, aiming to offer cross-field reference for researchers promote advancement research. Furthermore, we proposed novel reproducible paradigm, which can provide more trends trajectory field.

Язык: Английский

Процитировано

0

Application and progress of smart hydrogel microspheres for regulating oxidative stress in osteoarthritis DOI
Jinping Chen, Chengcheng Du, Bin Tang

и другие.

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160620 - 160620

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0

Topological cues of microparticles train stem cells for tissue repair via mechanotransduction DOI

Jiannan Mao,

Yichang Xu, Wenbo Wang

и другие.

Bioactive Materials, Год журнала: 2025, Номер 48, С. 531 - 549

Опубликована: Март 3, 2025

Язык: Английский

Процитировано

0

NebulaPlate: a droplet microfluidic platform to analyze platelet aggregation DOI Creative Commons

Zheyi Jiang,

Meng Wei, Jiawei Zhu

и другие.

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Март 5, 2025

The accurate assessment of platelet activity is crucial in clinical practice and scientific research owing to the pivotal role platelets progression cardiovascular conditions, such as arterial thrombotic diseases. However, conventional methods are currently limited by their requirement substantial blood samples inadequate high-throughput capabilities, therapeutic resistance induced antiplatelet agents impedes treatment efficacy. In this study, we developed a microdroplet-based function detection method, referred NebulaPlate, achieve miniaturized robust assessment, thereby overcoming current challenges. NebulaPlate supports merging with drugs confined picoliter microdroplets leverages an imaging-based analysis automatically identify platelets, evaluate aggregation, determine P-selectin expression within anchored microdroplets. We experimentally confirmed feasibility aggregation assays on using various representative drugs. Requiring only 0.3 mL whole blood/chip, which corresponds approximately 100 platelets/reaction, reduced consumption single assay. This represents reduction 10 times compared that techniques. Moreover, our experimental results validity reproducibility performed NebulaPlate. Our highlights important developments field provides fresh prospects for future therapies personalized medicine. it introduces new possibilities related

Язык: Английский

Процитировано

0

Recent advances of macroporous hydrogel microparticles: Fabrication and applications DOI
Yingzhe Liu,

Sida Ling,

Zhuo Chen

и другие.

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 161435 - 161435

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

A Narrative Review of Bioactive Hydrogel Microspheres: Ingredients, Modifications, Fabrications, Biological Functions, and Applications DOI Open Access
Haohui Li, Lili Yu, Ze Li

и другие.

Small, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

Abstract Hydrogel microspheres are important in regenerative medicine and tissue engineering, acting as cargos of cells, drugs, growth factors, bio‐inks for 3D printing, medical devices. The antimicrobial anti‐inflammatory characteristics hydrogel good treating injured tissues. However, the biological properties should be modified optimal treatment various body parts with different physiological biochemical environments. In addition, specific preparation methods required to produce customized shapes sizes clinical applications. Herein, advances biomedical applications reviewed. Synthesis precursor solutions, manufacturing methods, strategies enhancing functions these described. involvement bioactive repair is also discussed. This review anticipates fostering more insights into design, production, application biomedicine.

Язык: Английский

Процитировано

0

Cerium Nanozyme‐Powered Hydrogel Microspheres Alleviate Thromboangiitis Obliterans via Enhanced Stem Cell Therapy DOI
Yiqing Zhang,

Zhangpeng Shi,

Yimeng Shu

и другие.

Small, Год журнала: 2025, Номер unknown

Опубликована: Апрель 24, 2025

Abstract Thromboangiitis obliterans (TAO) is a chronic peripheral vascular condition characterized by thrombotic and inflammatory acceleration. As promising therapeutic modality for the TAO, mesenchymal stem cells (MSCs) transplantation expected to circumvent traditional drug therapy surgical interventions. Nonetheless, MSCs hindered owing poor survival, retention, engraftment of transplanted cells. The objective this research develop MSCs‐ cerium oxide nanoparticles (CeNPs)‐laden injectable methacrylated gelatin (GelMA)‐based hydrogel microspheres using microfluidics discern their potential regulate oxidative stress inflammation in rat model TAO. CeNPs‐loaded photocroslinkable GelMA not only protected against but also facilitated endothelial functional recovery, revascularization ischemic limb, suppression factors from macrophages. further conferred mechanical support prolonged residence time enhance efficacy cell therapy. In vivo study confirmed that combination CeNPs microsphere exhibited positive synergistic effect on tissue recovery angiogenesis. Taken together, work presents novel approach based integration nano‐micron combined microspheres, which may have implications TAO

Язык: Английский

Процитировано

0

Janus Hydrogels: Advanced Fabrication Techniques and Versatile Applications in Solar Evaporation, Biomedicine, and Electronic/Strain Sensors DOI
Yan Xue, Hong Xu, Hui Long

и другие.

ACS Applied Polymer Materials, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0

High-throughput Generation of Collagen Microbeads for 3D Cell Culture and Extracellular Vesicle Production DOI Creative Commons
Samantha Ali,

Fabiana Mastantuono,

Andrea Orozco-Torres

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 3, 2024

ABSTRACT Collagen type I, a fundamental component of natural extracellular matrices across species, is an attractive material for the development tissue engineering constructs. Nevertheless, poor mechanical properties and thermal instability hinder its use specific construction 3D cultures printing. In this study, we present microfluidic high-throughput approach producing high-quality uniform collagen microbeads without introducing any chemical modification. We achieved rapid droplet fabrication with sizes spanning from 50 µm to 1200 in production rate up 10000 droplets per minute. The resulting can serve as numerous microbioreactors which are suspended culture medium precipitation ideal cell growth. demonstrated excellent compatibility, facilitating attachment proliferation, well promoting vesicle secretion microbeads. This technology facile versatile high throughput culture, heterogeneous modeling, production, essential drug delivery screening.

Язык: Английский

Процитировано

0