Chemically Triggered Reactive Coacervates Show Life-Like Budding and Membrane Formation DOI Creative Commons
Sudeep Koppayithodi, Nishant Singh

Journal of the American Chemical Society, Год журнала: 2025, Номер unknown

Опубликована: Янв. 28, 2025

Phase-separated coacervates can enhance reaction kinetics and guide multilevel self-assembly, mimicking early cellular evolution. In this work, we introduce "reactive" complex that undergo chemically triggered self-immolative transformations, directing the self-assembly of products within their matrix. These self-assemblies then evolve to show life-like properties such as budding membrane formation. We find coacervate composition critically influences rates product distribution guides hierarchical self-assembly. This work showcases a versatile platform influence pathways for controlled supramolecular synthesis self-organization in confined spaces.

Язык: Английский

Self-assembly of stabilized droplets from liquid–liquid phase separation for higher-order structures and functions DOI Creative Commons
Mehwish Naz,

Lin Zhang,

Chong Chen

и другие.

Communications Chemistry, Год журнала: 2024, Номер 7(1)

Опубликована: Апрель 9, 2024

Dynamic microscale droplets produced by liquid-liquid phase separation (LLPS) have emerged as appealing biomaterials due to their remarkable features. However, the instability of limits construction population-level structures with collective behaviors. Here we first provide a brief background in context materials properties. Subsequently, discuss current strategies for stabilizing including physical and chemical modulation. We also recent development LLPS various applications such synthetic cells biomedical materials. Finally, give insights on how stabilized can self-assemble into higher-order displaying coordinated functions fully exploit potentials bottom-up biology applications.

Язык: Английский

Процитировано

21

Peptide-Mediated Liquid-Liquid Phase Separation and Biomolecular Condensates DOI

Guangle Li,

Chengqian Yuan, Xuehai Yan

и другие.

Soft Matter, Год журнала: 2025, Номер 21(10), С. 1781 - 1812

Опубликована: Янв. 1, 2025

Peptide-mediated liquid–liquid phase separation (LLPS) underpins the formation of dynamic biomolecular condensates, regulated by diverse molecular interactions, and highlights potential applications in drug delivery synthetic biology.

Язык: Английский

Процитировано

4

Continuous Transformation from Membrane‐less Coacervates to Membranized Coacervates and Giant Vesicles: toward Multicompartmental Protocells with Complex (Membrane) Architectures DOI Creative Commons
Yang Zhou, Kehu Zhang, Sílvia Moreno

и другие.

Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(34)

Опубликована: Июнь 7, 2024

The membranization of membrane-less coacervates paves the way for exploitation complex protocells with regard to structural and cell-like functional behaviors. However, controlled transformation from membranized vesicles remains a challenge. This can provide stable (multi)phase (multi)compartmental architectures through reconfiguration coacervate droplets in presence (bioactive) polymers, bio(macro)molecules and/or nanoobjects. Herein, we present continuous protocell and, ultimately, giant hybrid vesicles. process is orchestrated by altering balance non-covalent interactions varying concentrations an anionic terpolymer, leading dynamic processes such as spontaneous terpolymer nanoparticles at surface, disassembly phase mediated excess charge, redistribution components membrane. diverse during course distinct features molecular permeability. Notably, introduction multiphase this signifies advancements toward creation synthetic cells different diffusible compartments. Our findings emphasize highly reorganization represents novel step development advanced sophisticated more precise compositions (membrane) structures.

Язык: Английский

Процитировано

11

Multicompartment Synthetic Vesicles for Artificial Cell Applications DOI

Jooyong Shin,

Yeongseon Jang

ACS Applied Engineering Materials, Год журнала: 2024, Номер 2(12), С. 2758 - 2770

Опубликована: Март 21, 2024

This review explores the cutting-edge development of multicompartment synthetic vesicles designed for artificial cell applications, drawing inspiration from complex compartmentalization inherent in living cells. It delves into recent advancements engineering equipped with both membranous and membraneless organelles (vesicles-in-vesicles coacervates-in-vesicles), offering a detailed examination methodologies materials employed. paper highlights critical role these simulating cellular microenvironments functions, facilitating spatial temporal segregation biochemical processes, such as signal transduction, gene expression, ATP synthesis, energy production. Moreover, this outlines potential future directions, emphasizing importance evolution cells focus on their application creating more sophisticated biomimetic systems.

Язык: Английский

Процитировано

10

Coacervates Composed of Low‐Molecular‐Weight Compounds– Molecular Design, Stimuli Responsiveness, Confined Reaction DOI Creative Commons
Sayuri L. Higashi, Masato Ikeda

Advanced Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 12, 2025

The discovery of coacervation within living cells through liquid-liquid phase separation has inspired scientists to investigate its fundamental principles and significance. Indeed, coacervates composed low-molecular-weight compounds based on supramolecular strategy can offer valuable models for biomolecular condensates useful tools. This mini-review highlights recent findings advances in (artificial condensates), primarily compounds, with focuses their molecular design, stimuli responsiveness, controlled reactions or leading the coacervates.

Язык: Английский

Процитировано

2

Sequestration within peptide coacervates improves the fluorescence intensity, kinetics, and limits of detection of dye-based DNA biosensors DOI Creative Commons
Christopher M. Green, Deborah Sementa, Divita Mathur

и другие.

Communications Chemistry, Год журнала: 2024, Номер 7(1)

Опубликована: Фев. 29, 2024

Peptide-based liquid-liquid phase separated domains, or coacervates, are a biomaterial gaining new interest due to their exciting potential in fields ranging from biosensing drug delivery. In this study, we demonstrate that coacervates provide simple and biocompatible medium improve nucleic acid biosensors through the sequestration of both biosensor target strands within coacervate, thereby increasing local concentration. Using well-established polyarginine (R

Язык: Английский

Процитировано

9

Complex Coacervate-Based Materials for Biomedicine: Recent Advancements and Future Prospects DOI
Partha Sarathi Roy

Industrial & Engineering Chemistry Research, Год журнала: 2024, Номер 63(13), С. 5414 - 5487

Опубликована: Март 25, 2024

Complex coacervation, a specific type of associative phase separation that happens when oppositely charged macro-ions (or polyelectrolytes) are mixed, is the formation dense macro-ion-rich (the coacervate) in equilibrium with dilute macro-ion-poor continuous or supernatant). Since Bungenberg de Jong and colleagues' ground-breaking work on gelatin–acacia gum complex coacervation 1920s 1940s, coacervates have drawn increasing research attention because they essential to wide range established developing technologies. Charge complexation solution may play role this association. Unfortunately, misconceptions about theoretical foundations common, conceptual errors persisted literature. This Review an attempt provide concise comprehensive overview main streams pursued field regarding parameters influencing coacervates, encompassing various technological aspects enabling investigation industrial applications. Coacervate well-known widely used, but poorly understood, phenomenon physical polymer science. Finally, critical assessment perspectives anticipated difficulties further development improvement next-generation materials suggested, insights into field's future prospects explored.

Язык: Английский

Процитировано

9

Photoswitchable Endocytosis of Biomolecular Condensates in Giant Vesicles DOI
Agustín Mangiarotti, Mina Aleksanyan, Macarena Siri

и другие.

Advanced Science, Год журнала: 2024, Номер 11(23)

Опубликована: Апрель 6, 2024

Abstract Interactions between membranes and biomolecular condensates can give rise to complex phenomena such as wetting transitions, mutual remodeling, endocytosis. In this study, light‐triggered manipulation of condensate engulfment is demonstrated using giant vesicles containing photoswitchable lipids. UV irradiation increases the membrane area, which be stored in nanotubes. When contact with a droplet, light triggers rapid endocytosis, reverted by blue light. The affinity protein‐rich reversibility processes quantified from confocal microscopy images. degree photo‐induced engulfment, whether partial or complete, depends on vesicle excess area relative sizes condensates. Theoretical estimates suggest that utilizing light‐induced increase vesicle‐condensate adhesion interface energetically more favorable than energy gain folding into invaginations tubes. overall findings demonstrate membrane‐condensate interactions easily quickly modulated via light, providing versatile system for building platforms control cellular events design intelligent drug delivery systems cell repair.

Язык: Английский

Процитировано

8

Peptide-Based Biomimetic Condensates via Liquid–Liquid Phase Separation as Biomedical Delivery Vehicles DOI
Siyu Song, Tsvetomir Ivanov, Dandan Yuan

и другие.

Biomacromolecules, Год журнала: 2024, Номер 25(9), С. 5468 - 5488

Опубликована: Авг. 23, 2024

Biomolecular condensates are dynamic liquid droplets through intracellular liquid-liquid phase separation that function as membraneless organelles, which highly involved in various complex cellular processes and functions. Artificial analogs formed via similar pathways can be integrated with biological complexity advanced functions have received tremendous research interest the field of synthetic biology. The coacervate droplet-based compartments partition concentrate a wide range solutes, regarded attractive candidates for mimicking phase-separation behaviors biophysical features biomolecular condensates. use peptide-based materials phase-separating components has advantages such diversity amino acid residues customized sequence design, allows programming their physicochemical properties resulting compartments. In this Perspective, we highlight recent advancements design construction biomimicry from peptides relevant to protein, specific reference molecular self-assembly separation, biorelated applications, envisage emerging biomedical delivery vehicles.

Язык: Английский

Процитировано

8

Temporally controlled multistep division of DNA droplets for dynamic artificial cells DOI Creative Commons

Tomoya Maruyama,

Jing Gong, Masahiro Takinoue

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 27, 2024

Abstract Synthetic droplets mimicking bio-soft matter formed via liquid-liquid phase separation (LLPS) in living cells have recently been employed nanobiotechnology for artificial cells, molecular robotics, computing, etc. Temporally controlling the dynamics of synthetic is essential developing such bio-inspired systems because maintain their functions based on temporally controlled biomolecular reactions and assemblies. This paper reports temporal control DNA-based LLPS (DNA droplets). We demonstrate timing-controlled division DNA time-delayed triggers regulated by chemical reactions. Controlling release order multiple results multistep droplet division, i.e., pathway-controlled a reaction landscape. Finally, we apply into computing element to compare microRNA concentrations. believe that will promote design dynamic cells/molecular robots sophisticated biomedical applications.

Язык: Английский

Процитировано

8