Journal of the American Chemical Society,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 28, 2025
Phase-separated
coacervates
can
enhance
reaction
kinetics
and
guide
multilevel
self-assembly,
mimicking
early
cellular
evolution.
In
this
work,
we
introduce
"reactive"
complex
that
undergo
chemically
triggered
self-immolative
transformations,
directing
the
self-assembly
of
products
within
their
matrix.
These
self-assemblies
then
evolve
to
show
life-like
properties
such
as
budding
membrane
formation.
We
find
coacervate
composition
critically
influences
rates
product
distribution
guides
hierarchical
self-assembly.
This
work
showcases
a
versatile
platform
influence
pathways
for
controlled
supramolecular
synthesis
self-organization
in
confined
spaces.
Communications Chemistry,
Год журнала:
2024,
Номер
7(1)
Опубликована: Апрель 9, 2024
Dynamic
microscale
droplets
produced
by
liquid-liquid
phase
separation
(LLPS)
have
emerged
as
appealing
biomaterials
due
to
their
remarkable
features.
However,
the
instability
of
limits
construction
population-level
structures
with
collective
behaviors.
Here
we
first
provide
a
brief
background
in
context
materials
properties.
Subsequently,
discuss
current
strategies
for
stabilizing
including
physical
and
chemical
modulation.
We
also
recent
development
LLPS
various
applications
such
synthetic
cells
biomedical
materials.
Finally,
give
insights
on
how
stabilized
can
self-assemble
into
higher-order
displaying
coordinated
functions
fully
exploit
potentials
bottom-up
biology
applications.
Soft Matter,
Год журнала:
2025,
Номер
21(10), С. 1781 - 1812
Опубликована: Янв. 1, 2025
Peptide-mediated
liquid–liquid
phase
separation
(LLPS)
underpins
the
formation
of
dynamic
biomolecular
condensates,
regulated
by
diverse
molecular
interactions,
and
highlights
potential
applications
in
drug
delivery
synthetic
biology.
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
63(34)
Опубликована: Июнь 7, 2024
The
membranization
of
membrane-less
coacervates
paves
the
way
for
exploitation
complex
protocells
with
regard
to
structural
and
cell-like
functional
behaviors.
However,
controlled
transformation
from
membranized
vesicles
remains
a
challenge.
This
can
provide
stable
(multi)phase
(multi)compartmental
architectures
through
reconfiguration
coacervate
droplets
in
presence
(bioactive)
polymers,
bio(macro)molecules
and/or
nanoobjects.
Herein,
we
present
continuous
protocell
and,
ultimately,
giant
hybrid
vesicles.
process
is
orchestrated
by
altering
balance
non-covalent
interactions
varying
concentrations
an
anionic
terpolymer,
leading
dynamic
processes
such
as
spontaneous
terpolymer
nanoparticles
at
surface,
disassembly
phase
mediated
excess
charge,
redistribution
components
membrane.
diverse
during
course
distinct
features
molecular
permeability.
Notably,
introduction
multiphase
this
signifies
advancements
toward
creation
synthetic
cells
different
diffusible
compartments.
Our
findings
emphasize
highly
reorganization
represents
novel
step
development
advanced
sophisticated
more
precise
compositions
(membrane)
structures.
ACS Applied Engineering Materials,
Год журнала:
2024,
Номер
2(12), С. 2758 - 2770
Опубликована: Март 21, 2024
This
review
explores
the
cutting-edge
development
of
multicompartment
synthetic
vesicles
designed
for
artificial
cell
applications,
drawing
inspiration
from
complex
compartmentalization
inherent
in
living
cells.
It
delves
into
recent
advancements
engineering
equipped
with
both
membranous
and
membraneless
organelles
(vesicles-in-vesicles
coacervates-in-vesicles),
offering
a
detailed
examination
methodologies
materials
employed.
paper
highlights
critical
role
these
simulating
cellular
microenvironments
functions,
facilitating
spatial
temporal
segregation
biochemical
processes,
such
as
signal
transduction,
gene
expression,
ATP
synthesis,
energy
production.
Moreover,
this
outlines
potential
future
directions,
emphasizing
importance
evolution
cells
focus
on
their
application
creating
more
sophisticated
biomimetic
systems.
Advanced Biology,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 12, 2025
The
discovery
of
coacervation
within
living
cells
through
liquid-liquid
phase
separation
has
inspired
scientists
to
investigate
its
fundamental
principles
and
significance.
Indeed,
coacervates
composed
low-molecular-weight
compounds
based
on
supramolecular
strategy
can
offer
valuable
models
for
biomolecular
condensates
useful
tools.
This
mini-review
highlights
recent
findings
advances
in
(artificial
condensates),
primarily
compounds,
with
focuses
their
molecular
design,
stimuli
responsiveness,
controlled
reactions
or
leading
the
coacervates.
Communications Chemistry,
Год журнала:
2024,
Номер
7(1)
Опубликована: Фев. 29, 2024
Peptide-based
liquid-liquid
phase
separated
domains,
or
coacervates,
are
a
biomaterial
gaining
new
interest
due
to
their
exciting
potential
in
fields
ranging
from
biosensing
drug
delivery.
In
this
study,
we
demonstrate
that
coacervates
provide
simple
and
biocompatible
medium
improve
nucleic
acid
biosensors
through
the
sequestration
of
both
biosensor
target
strands
within
coacervate,
thereby
increasing
local
concentration.
Using
well-established
polyarginine
(R
Industrial & Engineering Chemistry Research,
Год журнала:
2024,
Номер
63(13), С. 5414 - 5487
Опубликована: Март 25, 2024
Complex
coacervation,
a
specific
type
of
associative
phase
separation
that
happens
when
oppositely
charged
macro-ions
(or
polyelectrolytes)
are
mixed,
is
the
formation
dense
macro-ion-rich
(the
coacervate)
in
equilibrium
with
dilute
macro-ion-poor
continuous
or
supernatant).
Since
Bungenberg
de
Jong
and
colleagues'
ground-breaking
work
on
gelatin–acacia
gum
complex
coacervation
1920s
1940s,
coacervates
have
drawn
increasing
research
attention
because
they
essential
to
wide
range
established
developing
technologies.
Charge
complexation
solution
may
play
role
this
association.
Unfortunately,
misconceptions
about
theoretical
foundations
common,
conceptual
errors
persisted
literature.
This
Review
an
attempt
provide
concise
comprehensive
overview
main
streams
pursued
field
regarding
parameters
influencing
coacervates,
encompassing
various
technological
aspects
enabling
investigation
industrial
applications.
Coacervate
well-known
widely
used,
but
poorly
understood,
phenomenon
physical
polymer
science.
Finally,
critical
assessment
perspectives
anticipated
difficulties
further
development
improvement
next-generation
materials
suggested,
insights
into
field's
future
prospects
explored.
Abstract
Interactions
between
membranes
and
biomolecular
condensates
can
give
rise
to
complex
phenomena
such
as
wetting
transitions,
mutual
remodeling,
endocytosis.
In
this
study,
light‐triggered
manipulation
of
condensate
engulfment
is
demonstrated
using
giant
vesicles
containing
photoswitchable
lipids.
UV
irradiation
increases
the
membrane
area,
which
be
stored
in
nanotubes.
When
contact
with
a
droplet,
light
triggers
rapid
endocytosis,
reverted
by
blue
light.
The
affinity
protein‐rich
reversibility
processes
quantified
from
confocal
microscopy
images.
degree
photo‐induced
engulfment,
whether
partial
or
complete,
depends
on
vesicle
excess
area
relative
sizes
condensates.
Theoretical
estimates
suggest
that
utilizing
light‐induced
increase
vesicle‐condensate
adhesion
interface
energetically
more
favorable
than
energy
gain
folding
into
invaginations
tubes.
overall
findings
demonstrate
membrane‐condensate
interactions
easily
quickly
modulated
via
light,
providing
versatile
system
for
building
platforms
control
cellular
events
design
intelligent
drug
delivery
systems
cell
repair.
Biomacromolecules,
Год журнала:
2024,
Номер
25(9), С. 5468 - 5488
Опубликована: Авг. 23, 2024
Biomolecular
condensates
are
dynamic
liquid
droplets
through
intracellular
liquid-liquid
phase
separation
that
function
as
membraneless
organelles,
which
highly
involved
in
various
complex
cellular
processes
and
functions.
Artificial
analogs
formed
via
similar
pathways
can
be
integrated
with
biological
complexity
advanced
functions
have
received
tremendous
research
interest
the
field
of
synthetic
biology.
The
coacervate
droplet-based
compartments
partition
concentrate
a
wide
range
solutes,
regarded
attractive
candidates
for
mimicking
phase-separation
behaviors
biophysical
features
biomolecular
condensates.
use
peptide-based
materials
phase-separating
components
has
advantages
such
diversity
amino
acid
residues
customized
sequence
design,
allows
programming
their
physicochemical
properties
resulting
compartments.
In
this
Perspective,
we
highlight
recent
advancements
design
construction
biomimicry
from
peptides
relevant
to
protein,
specific
reference
molecular
self-assembly
separation,
biorelated
applications,
envisage
emerging
biomedical
delivery
vehicles.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Авг. 27, 2024
Abstract
Synthetic
droplets
mimicking
bio-soft
matter
formed
via
liquid-liquid
phase
separation
(LLPS)
in
living
cells
have
recently
been
employed
nanobiotechnology
for
artificial
cells,
molecular
robotics,
computing,
etc.
Temporally
controlling
the
dynamics
of
synthetic
is
essential
developing
such
bio-inspired
systems
because
maintain
their
functions
based
on
temporally
controlled
biomolecular
reactions
and
assemblies.
This
paper
reports
temporal
control
DNA-based
LLPS
(DNA
droplets).
We
demonstrate
timing-controlled
division
DNA
time-delayed
triggers
regulated
by
chemical
reactions.
Controlling
release
order
multiple
results
multistep
droplet
division,
i.e.,
pathway-controlled
a
reaction
landscape.
Finally,
we
apply
into
computing
element
to
compare
microRNA
concentrations.
believe
that
will
promote
design
dynamic
cells/molecular
robots
sophisticated
biomedical
applications.
