Fallopian tube rheology regulates epithelial cell differentiation and function to enhance cilia formation and coordination
Melati S. Abdul Halim,
Jennifer M. Dyson,
Max M. Gong
и другие.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Авг. 28, 2024
The
rheological
properties
of
the
extracellular
fluid
in
female
reproductive
tract
vary
spatiotemporally,
however,
effect
on
behaviour
epithelial
cells
that
line
is
unexplored.
Here,
we
reveal
respond
to
elevated
viscosity
culture
media
by
modulating
their
development
and
functionality
enhance
cilia
formation
coordination.
Specifically,
ciliation
increases
4-fold
beating
frequency
decreases
30%
when
are
cultured
at
100
mPa·s.
Further,
manifest
a
coordinated
pattern
can
facilitate
metachronal
waves.
At
cellular
level,
viscous
loading
activates
TRPV4
channel
increase
intracellular
Ca
Язык: Английский
Advancements in Microfluidic Technologies for Male Infertility
Advanced Materials Technologies,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 7, 2025
Abstract
Infertility
affects
≈15%
of
couples
worldwide,
with
≈45%
these
cases
involving
male
factors.
Semen
analysis
and
sperm
selection
are
critical
routine
steps
in
achieving
successful
assisted
reproductive
outcomes.
Conventional
methods,
which
widely
used
clinics,
manual,
subjective,
time‐consuming,
simply
not
sufficient
for
the
highly
complex
multifaceted
task
analysis.
Recently,
microfluidics‐based
devices,
combined
high‐resolution
microscopy,
have
offered
promising
opportunities
evaluating
quality,
gaining
a
fundamental
understanding
motion,
high‐quality
sperm.
Machine
learning
(ML)
has
also
introduced
automation
standardization
analyzing
morphology,
intracellular
characteristics,
motility.
In
this
review,
state‐of‐the‐art
methods
comprehensively
discussed
provide
directions
to
address
unresolved
challenges.
Язык: Английский
Superhelix flow structures drive sperm locomotion
Cell Reports Physical Science,
Год журнала:
2025,
Номер
unknown, С. 102524 - 102524
Опубликована: Апрель 1, 2025
Язык: Английский
Straining Flow Effects on Sperm Flagellar Energetics in Microfluidic Cross‐Slot Traps
Small,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 17, 2025
Abstract
Sperm
need
to
effectively
navigate
the
intricate
pathways
of
female
reproductive
tract,
which
are
filled
with
various
complex
fluid
flows.
Despite
numerous
population‐based
studies,
effects
flow
on
flagellar
beating
pattern
individual
sperm
remain
poorly
understood.
In
this
study,
a
microfluidic
cross‐slot
trap
is
employed
immobilize
motile
for
an
extended
period
without
physical
tethering,
thereby
reducing
potential
cell
damage
and
movement
restriction
compared
conventional
head‐tethering
method.
The
impact
pure
straining
trapped
single
investigated.
experimental
results
demonstrate
that
at
strain
rates
11.33
s
−1
higher,
periodic
repetitive
flagellum
changes
irregular
movement.
Furthermore,
increase
in
rate
from
1.89
leads
35.4%
reduction
amplitude
41.2%
decrease
hydrodynamic
power
dissipation.
These
findings
underscore
capability
platform
high
stability,
contributing
better
understanding
behavior
response
Язык: Английский
A Neuromorphic Camera for Tracking Passive and Active Matter with Lower Data Throughput
Laser & Photonics Review,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 29, 2025
Abstract
The
merits
of
using
a
neuromorphic,
or
event‐based
camera
(EBC),
for
tracking
both
passive
and
active
matter
are
demonstrated.
For
matter,
the
Brownian
motion
different
micro‐particles
is
tracked
their
diffusion
coefficients
estimated.
case
murine
spermatozoa
explored
motility
parameters
extracted
from
cells.
This
has
applications
in
enhancing
outcomes
clinical
fertility
treatments.
Using
EBC,
results
equivalent
to
those
an
sCMOS
obtained,
while
achieving
reduction
file
size
up
two
orders
magnitude.
important
modern
computer
era,
as
it
reduces
data
throughput,
well‐aligned
with
edge‐computing
applications.
EBC
considered
excellent
choice,
particularly
long‐term
studies
matter.
Язык: Английский
Elastohydrodynamic mechanisms govern beat pattern transitions in eukaryotic flagella
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 8, 2024
Abstract
Eukaryotic
cilia
and
flagella
exhibit
complex
beating
patterns
that
vary
depending
on
environmental
conditions
such
as
fluid
viscosity
1
.
These
transitions
are
thought
to
arise
from
changes
in
the
internal
forcing
provided
by
axoneme,
although
mechanism
remains
unclear
2,3
We
demonstrate
with
simulations
of
Kirchhoff
rods
driven
internally
active
bending
moments
a
single
elastohydrodynamic
instability
universally
explains
between
planar,
quasiplanar,
helical,
due
either
forcing,
flagellar
stiffness
length,
or
hydrodynamic
resistance,
ambient
medium
presence
plane
wall.
The
beat
comparable
those
exhibited
bull
sperm
sea
urchin
our
experiments
elsewhere
3–5
Our
results
point
general
model
can
describe
ciliary
across
all
species.
further
show
dynein
forces
be
estimated
comparing
simulation
experimental
observations
transitional
viscosities.
This
potentially
lead
diagnostic
assays
measure
health
cells
based
their
pattern.
Язык: Английский