Elastohydrodynamic mechanisms govern beat pattern transitions in eukaryotic flagella DOI Creative Commons
Shibani Veeraragavan, Farin Yazdan Parast, Reza Nosrati

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 8, 2024

Abstract Eukaryotic cilia and flagella exhibit complex beating patterns that vary depending on environmental conditions such as fluid viscosity 1 . These transitions are thought to arise from changes in the internal forcing provided by axoneme, although mechanism remains unclear 2,3 We demonstrate with simulations of Kirchhoff rods driven internally active bending moments a single elastohydrodynamic instability universally explains between planar, quasiplanar, helical, due either forcing, flagellar stiffness length, or hydrodynamic resistance, ambient medium presence plane wall. The beat comparable those exhibited bull sperm sea urchin our experiments elsewhere 3–5 Our results point general model can describe ciliary across all species. further show dynein forces be estimated comparing simulation experimental observations transitional viscosities. This potentially lead diagnostic assays measure health cells based their pattern.

Language: Английский

Fallopian tube rheology regulates epithelial cell differentiation and function to enhance cilia formation and coordination DOI Creative Commons

Melati S. Abdul Halim,

Jennifer M. Dyson,

Max M. Gong

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 28, 2024

The rheological properties of the extracellular fluid in female reproductive tract vary spatiotemporally, however, effect on behaviour epithelial cells that line is unexplored. Here, we reveal respond to elevated viscosity culture media by modulating their development and functionality enhance cilia formation coordination. Specifically, ciliation increases 4-fold beating frequency decreases 30% when are cultured at 100 mPa·s. Further, manifest a coordinated pattern can facilitate metachronal waves. At cellular level, viscous loading activates TRPV4 channel increase intracellular Ca

Language: Английский

Citations

7
Advancements in Microfluidic Technologies for Male Infertility DOI Open Access
Sahar Shahali, Farzan Akbaridoust, Adrian Neild

et al.

Advanced Materials Technologies, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Abstract Infertility affects ≈15% of couples worldwide, with ≈45% these cases involving male factors. Semen analysis and sperm selection are critical routine steps in achieving successful assisted reproductive outcomes. Conventional methods, which widely used clinics, manual, subjective, time‐consuming, simply not sufficient for the highly complex multifaceted task analysis. Recently, microfluidics‐based devices, combined high‐resolution microscopy, have offered promising opportunities evaluating quality, gaining a fundamental understanding motion, high‐quality sperm. Machine learning (ML) has also introduced automation standardization analyzing morphology, intracellular characteristics, motility. In this review, state‐of‐the‐art methods comprehensively discussed provide directions to address unresolved challenges.

Language: Английский

Citations

0
Superhelix flow structures drive sperm locomotion DOI Creative Commons
Farzan Akbaridoust,

Melati S. Abdul Halim,

Ivan Marušič

et al.

Cell Reports Physical Science, Journal Year: 2025, Volume and Issue: unknown, P. 102524 - 102524

Published: April 1, 2025

Language: Английский

Citations

0
Straining Flow Effects on Sperm Flagellar Energetics in Microfluidic Cross‐Slot Traps DOI Creative Commons
Javane Javaherchian, Farin Yazdan Parast, Reza Nosrati

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Abstract Sperm need to effectively navigate the intricate pathways of female reproductive tract, which are filled with various complex fluid flows. Despite numerous population‐based studies, effects flow on flagellar beating pattern individual sperm remain poorly understood. In this study, a microfluidic cross‐slot trap is employed immobilize motile for an extended period without physical tethering, thereby reducing potential cell damage and movement restriction compared conventional head‐tethering method. The impact pure straining trapped single investigated. experimental results demonstrate that at strain rates 11.33 s −1 higher, periodic repetitive flagellum changes irregular movement. Furthermore, increase in rate from 1.89 leads 35.4% reduction amplitude 41.2% decrease hydrodynamic power dissipation. These findings underscore capability platform high stability, contributing better understanding behavior response

Language: Английский

Citations

0
A Neuromorphic Camera for Tracking Passive and Active Matter with Lower Data Throughput DOI Creative Commons
Gabriel Britto Monteiro, Megan Lim, Tiffany C. Y. Tan

et al.

Laser & Photonics Review, Journal Year: 2025, Volume and Issue: unknown

Published: May 29, 2025

Abstract The merits of using a neuromorphic, or event‐based camera (EBC), for tracking both passive and active matter are demonstrated. For matter, the Brownian motion different micro‐particles is tracked their diffusion coefficients estimated. case murine spermatozoa explored motility parameters extracted from cells. This has applications in enhancing outcomes clinical fertility treatments. Using EBC, results equivalent to those an sCMOS obtained, while achieving reduction file size up two orders magnitude. important modern computer era, as it reduces data throughput, well‐aligned with edge‐computing applications. EBC considered excellent choice, particularly long‐term studies matter.

Language: Английский

Citations

0
Elastohydrodynamic mechanisms govern beat pattern transitions in eukaryotic flagella DOI Creative Commons
Shibani Veeraragavan, Farin Yazdan Parast, Reza Nosrati

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 8, 2024

Abstract Eukaryotic cilia and flagella exhibit complex beating patterns that vary depending on environmental conditions such as fluid viscosity 1 . These transitions are thought to arise from changes in the internal forcing provided by axoneme, although mechanism remains unclear 2,3 We demonstrate with simulations of Kirchhoff rods driven internally active bending moments a single elastohydrodynamic instability universally explains between planar, quasiplanar, helical, due either forcing, flagellar stiffness length, or hydrodynamic resistance, ambient medium presence plane wall. The beat comparable those exhibited bull sperm sea urchin our experiments elsewhere 3–5 Our results point general model can describe ciliary across all species. further show dynein forces be estimated comparing simulation experimental observations transitional viscosities. This potentially lead diagnostic assays measure health cells based their pattern.

Language: Английский

Citations

0