Therapeutic potential of hydrogen-rich water in zebrafish model of Alzheimer’s disease: targeting oxidative stress, inflammation, and the gut-brain axis

Frontiers in Aging Neuroscience, Год журнала: 2025, Номер 16

Опубликована: Янв. 7, 2025

Alzheimer's disease (AD) is a complex neurodegenerative disorder, with amyloid-beta (Aβ) aggregation playing key role in its pathogenesis. Aβ-induced oxidative stress leads to neuronal damage, mitochondrial dysfunction, and apoptosis, making antioxidative strategies promising for AD treatment. This study investigates the effects of hydrogen-rich water (HRW) zebrafish model. Zebrafish were exposed aluminum chloride induce AD-like pathology then treated HRW using nanobubble device. Behavioral assays, ELISA, Hematoxylin-eosin (H&E) staining, reactive oxygen species (ROS) neutrophil fluorescence labeling employed assess HRW's impact. Additionally, 16S rRNA sequencing analyzed effect on gut microbiota. can significantly improve cognitive impairment depression-like behavior model, reduce Aβ deposition (p < 0.0001), regulate liver Soluble epoxide hydrolase (sEH) levels 0.05), neuroinflammation, stress. Furthermore, reduced number harmful bacteria linked by restoring balance microbiota gut. These findings suggest that has potential as therapeutic strategy targeting stress, inflammation, gut-brain axis modulation.

Язык: Английский

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Macrophage-derived exosomes in cancer: a double-edged sword with therapeutic potential

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Апрель 26, 2025

Solid cancer contains a complicated communication network between cells and components in the tumor microenvironment (TME), significantly influencing progression of cancer. Exosomes function as key carriers signaling molecules these communications, including intricate signalings tumor-associated macrophages (TAMs) on TME. With their natural lipid bilayer structures biological activity that relates to original cell, exosomes have emerged efficient studies therapy. Intrigued by heterogeneity plasticity both exosomes, we regard macrophage-derived double-edged sword. For instance, TAM-derived educated TME, can promote resistance therapies, while generated vitro shown favorable potential Here, depict reasons for well manifold roles progression, metastasis, In particular, emphasize recent advancements modified diverse arguing are endowed with unique advantages macrophage origin. We outline challenges translating scientific discoveries into clinical therapy, aiming provide patients safe effective treatments.

Язык: Английский

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Gas immnuo-nanomedicines fight cancers

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 180, С. 117595 - 117595

Опубликована: Окт. 31, 2024

Certain gas molecules, including hydrogen (H

Язык: Английский

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Biodegradable Ruthenium‐Rhenium Complexes Containing Nanoamplifiers: Triggering ROS‐Induced CO Release for Synergistic Cancer Treatment

Advanced Science, Год журнала: 2024, Номер unknown

Опубликована: Июль 12, 2024

Abstract The constrained effectiveness of photodynamic therapy (PDT) has impeded its widespread use in clinical practice. Urgent efforts are needed to address the shortcomings faced therapy, such as photosensitizer toxicity, short half‐life, and limited action range reactive oxygen species (ROS). In this study, a biodegradable copolymer nanoamplifier is reported that contains ruthenium complex (Ru‐complex) (PS) rhenium (Re‐complex) carbon monoxide (CO)‐release molecule (CORM). well‐designed brings PS CORM into close spatial proximity, significantly promotes utilization light‐stimulated (ROS), cascaded amplifying CO release, thus enabling an enhanced synergistic effect PDT gas for cancer treatment. Moreover, owing intrinsic photodegradable nature, exhibits good tumor accumulation penetration ability, excellent biocompatibility vivo. These findings suggest nanoamplifiers pave way clinically viable integration therapy.

Язык: Английский

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Recent Advances in Silica-Based Nanomaterials for Enhanced Tumor Imaging and Therapy

ACS Applied Bio Materials, Год журнала: 2024, Номер 7(11), С. 7133 - 7169

Опубликована: Ноя. 4, 2024

Cancer remains a formidable challenge, inflicting profound physical, psychological, and financial burdens on patients. In this context, silica-based nanomaterials have garnered significant attention for their potential in tumor imaging therapy owing to exceptional properties, such as biocompatibility, customizable porosity, versatile functionalization capabilities. This review meticulously examines the latest advancements application of therapy. It underscores enhancing various cancer modalities, including fluorescence imaging, magnetic resonance computed tomography, positron emission ultrasound multimodal approaches. Moreover, delves into therapeutic efficacy chemotherapy, radiotherapy, phototherapy, immunotherapy, gas therapy, sonodynamic chemodynamic starvation gene Critical evaluations biosafety profiles degradation pathways these within biological environments are also presented. By discussing current challenges prospects, aims provide nuanced perspective clinical translation nanomaterials, thereby highlighting promise revolutionizing diagnostics, enabling real-time monitoring responses, advancing personalized medicine.

Язык: Английский

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Synchronous Interference of Dual Metabolic Pathways Mediated by H₂S Gas/GOx for Augmenting Tumor Microwave Thermal Therapy

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Янв. 11, 2025

Sublethal tumor cells have an urgent need for energy, making it common them to switch metabolic phenotypes between glycolysis and oxidative phosphorylation (OXPHOS) compensatory energy supply; thus, the synchronous interference of dual pathways limiting level is essential in inhibiting sublethal growth. Herein, a multifunctional nanoplatform Co-MOF-loaded anethole trithione (ADT) myristyl alcohol (MA), modified with GOx hyaluronic acid (HA) was developed, namely, CAMGH. It could synchronously interfere including OXPHOS restrict adenosine triphosphate (ATP) supply, achieving inhibition tumors after microwave (MW) thermal therapy. Under low-power MW irradiation, CAMGH induced certain damage while ensuring safety surrounding normal tissues. The loaded consumed glucose tumors, undoubtedly blocking main supply pathway, glycolytic pathway. Then, H

Язык: Английский

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Piezocatalysis for gas generation and disease therapy

Medical Gas Research, Год журнала: 2025, Номер 15(2), С. 202 - 203

Опубликована: Янв. 18, 2025

In recent years, gases such as oxygen (O2), nitric oxide (NO), carbon monoxide (CO), dioxide (CO2), hydrogen sulfide (H2S), and (H2) have been found to the biological function influence a variety of physiological processes in human body hold potential for range biomedical applications, including treatment cancer inflammatory diseases.1 For example, H2 therapy can protect against oxidative stress inflammation, supporting heart health, while NO is effective its vasodilatory role managing hypertension. treatment, CO H2S show controlling tumor growth. Gas also offers anti-inflammatory immunomodulatory benefits, potentially remodeling chronic conditions. Consequently, an emerging therapeutic approach known gas has garnered significant attention. Unlike traditional drugs, molecules are less likely induce therapy-resistance better tissue permeability solid tissues, positioning promising strategy supplementing other modalities. However, extremely short half-life uncontrolled distribution pose challenges safety targeted application, highlighting urgent need therapies with precisely controllable release pathways.2 Piezocatalysis, catalytic method, typically utilizes piezoelectric materials generate activity through mechanical stress.3 When subjected external forces pressure or vibration, these internal electric field that drives reactions. early research, piezocatalysis received attention applications environmental remediation, particularly degradation organic pollutants production clean energy water splitting.4 Its unique ability under stimuli without light chemicals makes it sustainable solutions protection. The ongoing research exploring potentials well. Due spatiotemporal controllability piezocatalysis, enables targeted, spatio-temporal, precise generation therapy. As result, use disease emerged direction. This perspective will summarize advances future prospects driven by (Figure 1).Figure 1: Schematic diagram treatment.The depicts process from initiation effect disease, well advantages piezocatalytic therapy.Brief principles piezocatalysis: 1880, Curie brothers, Pierre Jacques, discovered effect. non-centrosymmetric structure stress, crystal undergoes polarization. polarization causes negative positive charges accumulate on opposite surfaces crystals. forms across material boundaries. phenomenon effect.5 2010, Hong et al.6 reported zinc (ZnO) barium titanate (BaTiO3) could decompose ultrasound (US) irradiation proposed concept chemical effect, which was later termed piezocatalysis. Currently, two widely recognized mechanisms band theory charge screening effect.7 According theory, regulates controls migration carriers surface catalyst. piezocatalysts specific reactions primarily determined states valence conduction bands. deform stimulation, displacement centers leads resulting distributed sides participate instance, electron (e–) reduction H+ produce hole (h+) oxidation OH– hydroxyl radicals (•OH). regard acts driving force this process, involved redox come surface-adsorbed environment, rather than internally generated within material. presence field, attracted neutralize polarized bound charges, then dissociate, electrons both mechanisms, strain-induced regulate process. Therefore, be fully controlled forces, granting temporal spatial precision. generating therapies. Recent therapy: Hydrogen inflammation: previous study, appropriate dose proven capable selectively reducing harmful free radicals, thereby alleviating inflammation.1 Additionally, localized delivery wound sites able activate immune responses, promoting healing, demonstrates H2. You al.8 developed gold nanoparticle-modified BaTiO3 nanoparticles enhance Schottky heterojunction's performance production. bare nanoparticles, metal-semiconductor composite exhibited strong continuously produced aqueous solution US acute bone marrow injury 2A). piezocatalysis-based effectively cleared intracellular reactive species alleviate upregulating expression antioxidant enzymes anti-apoptotic genes. activated tryptophan metabolism pathway, leading reprogramming microenvironment protecting neurons apoptosis.Figure 2: Overview therapy.(A) representation spinal cord using Au@BT generator. Reprinted Copyright 2024 Wiley‐VCH GmbH. (B) depiction hydrogen/oxygen based complete splitting via ultrasound-triggered Xia al.9 2023 Elsevier Ltd. (C) illustration SnS nanosheets-mediated piezoelectrocatalytic lactic acid deprivation immunoactivation. Wu al.10 (D) enhanced chemotherapy inhibition stromal barriers improved CPT penetration combined nanoprodrug strategy. Wang al.11 American Chemical Society. (E) BST@LA synergistic sonodynamic Reproduced permission. Zhao al.12 Au@BT: Gold titanate; BST@LA: L-arginine modified strontium-doped CB: band; CPT: camptothecin; CRB: CPT-t-R-PEG2000@BaTiO3; H2: hydrogen; I.V.: intravenous; IL: interleukin; LA: L-arginine; NO: oxide; O2: oxygen; PA: pyruvic acid; PD-L1: programmed death-ligand 1; ROS: species; SDT: therapy; SnS: tin sulfide; TNF-α: necrosis factor α; US: ultrasound; VB: band.Hydrogen addition clearing possesses locally immunity, offering immunotherapy. To achieve deep tumors, they mesoporous sulphur (ZnS) enable efficient piezocatalysis-induced splitting, providing sustained O2 at site. induced intratumoral macrophage toward pro-inflammatory M1 phenotype, alleviated hypoxia tumor-killing CD8+ T cells, ultimately deep-seated tumors 2B). Shortly thereafter, US-driven two-dimensional (SnS) nanosheets excellent properties, enabling tumors.10 situ liver model, catalyzed stimulation consumed excess 2C). immunologically inhibited cells immunotherapy intercepting 1/programmed death 1 (PD-L1/PD-1) immunosuppressive regulatory tumors. Nitric endogenous gaseous signaling molecule, shows great therapy.1 At high concentrations, disrupts cellular mitochondria, impairs supply, inhibits cell proliferation. low act modulator P-glycoprotein, multidrug resistance. Controlled on-demand potential. US-responsive nanoplatform amphiphilic prodrug consisted drug camptothecin donor linked thioketal bond. Based US-triggered hypoxic microenvironment, initiating series chain These broke bonds, releasing oxidizing 2D). results demonstrated achieved vivo NO, removing tumor's fibrotic stroma, enhancing penetration, achieving dense Li's group12 fabricated BaTiO₃ introducing vacancies into improving their intrinsic reduced gap carriers. Under highly functionalized species, decomposition surface-bound site 2E). Ultimately, resulted high-efficient treatment. Conclusion perspective: method effects O2, H2, treat diseases. It overcomes resistance poor often encountered therapies, making field. remains major challenge clinical application. involves separation electron-hole pairs promote reactions, suitable gases. paper reviews progress Current studies gas-based treatments; however, still stages development, there several issues addressed researches. First, although displayed broad medical science technology, action living organisms further crucial breakthroughs researches mainly vitro experimental settings, underlying exploration. Moreover, impact complex diverse, hinders translation Second, difficult accurately determine amount body, no unified standard minimum required key indicator therapy, but current focus quantifying levels experiments due measuring vivo. clear indicators optimal effects, requires evaluation. Lastly, biocompatibility evaluation translation. cytotoxicity, distribution, retention, thoroughly studied optimized. may affect process; formation rupture microbubbles cavitation photocatalysis. Such coupling require conclusion, brings new possibilities delivery. By provides pathway advancing applications. Although some overcome, continued pave way innovation improve healthcare. work supported National Nature Science Foundation China (No. 82072065), Fundamental Research Funds Central Universities E2EG6802X2), Youth Talent Support Program China. Open access statement:This open journal, articles terms Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, allows others remix, tweak, build upon non-commercially, long credit given creations licensed identical terms.

Язык: Английский

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An Enhanced Osseointegration of Titanium Implants by H₂S Sustained‐Release Coating via Promoting Osteogenesis and Inhibiting Osteoclastogenesis

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 12, 2025

Abstract The surfaces of titanium implants lack the ability promoting osteogenesis and inhibiting osteoclastogenesis, which primarily contributes to their inadequate integration with surrounding bone tissue in osteoporotic environments. Developing a bioactive coating for materials is regarded as promising approach address challenge. In this study, hydrogen sulfide (H 2 S) sustained‐release fabricated on implants. consists bovine serum albumin nanoparticles encapsulating H S donor morpholin‐4‐ium(4‐methoxyphenyl)‐morpholin‐4‐ylsulfanylidenesulfido‐λ 5 ‐phosphane (GYY4137), prepared participation dopamine self‐polymerization. release rate could be precisely controlled by adjusting crosslinking degree nanoparticles. proves have excellent biocompatibility satisfactory hemocompatibility. vitro, significantly promote osteogenic differentiation marrow mesenchymal stem cells (BMSCs) inhibit osteoclastic mouse monocytic macrophage leukemia (RAW264.7 cells). vivo, work successfully verifies conclusion obtained vitro experiments. restores homeostasis imbalance functionalized femurs rats, thereby accelerating new formation enhancing osseointegration capability This provides practical strategy development suitable osteoporosis patients.

Язык: Английский

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Ultrasound-Driven Nitric Oxide Generation for Enhanced Sonodynamic–Photothermal Therapy

Molecular Pharmaceutics, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Recently, green gas therapy based on nitric oxide (NO) has gained considerable attention in cancer treatment. The supplementation of exogenous NO and its controlled release represent promising strategies for adjuvant tumor therapy. In this study, we developed a novel ultrasound (US)-triggered generation nanoplatform that integrates therapy, sonodynamic photothermal (PTT) into collaborative therapeutic modality. An environmentally friendly biomacromolecule, polydopamine, was employed to coload chlorin e6 (Ce6) donor (BNN6), resulting the nanocomposite PDA-Ce6/BNN6 (PCB). A single US stimulus simultaneously activated Ce6 produce reactive oxygen species (ROS) promoted BNN6 NO. dual effects ultrasonic mechanical action physiological modulation by substantially improved local vascular function enhanced cell permeability, thereby increasing targeted accumulation PCB within tumors. Reactive nitrogen (RNS) derived from ROS further exacerbated oxidative damage sensitivity cells hyperthermia. Both vitro vivo experiments demonstrated stimulation NO/ROS/RNS combined with PTT effectively inhibited growth proliferation. findings suggest extracorporeal can significantly amplify efficacy offer new insights developing other aimed at physically regulating deep

Язык: Английский

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Gasotransmitters in Modern Medicine: Promises and Challenges in the Use of Porous Crystalline Carriers

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Gas therapy using gasotransmitters is of exponential interest due to the growing recognition gas signaling molecules that can be involved in multiple therapeutic actions. Finding suitable methods for delivering these crucial, and porous materials, notably metal-organic frameworks (MOFs), have shown exceptional potential as carriers, enabling safe controlled local delivery. The challenges translate MOF therapies clinical use require not only validate mechanisms action pathways released but also get a deep understanding into rational design, it requires combination features maximize outcomes. In this perspective, we outline key criteria designing MOFs delivery applications highlight expanding range opportunities materials may offer. Readers insights design develop gasotransmitter with realistic clinic interest.

Язык: Английский

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