Comprehensive Quality Assessment of Portulacae Herba by HPLC Fingerprint and Multiple Chemometric Analysis
Separation Science Plus,
Год журнала:
2025,
Номер
8(5)
Опубликована: Май 1, 2025
ABSTRACT
Portulacae
Herba
is
the
dry
ground
part
of
Portulaca
oleracea
L.
It
an
annual,
herbaceous
and
succulent
plant
that
has
been
used
worldwide
as
both
food
medicine
for
many
years.
However,
some
market
products
exhibit
low
concentrations
active
compounds
nutritional
value,
leading
to
unsatisfactory
effects
a
cosmetic
ingredient
or
traditional
Chinese
medicine.
This
study
uses
high‐performance
liquid
chromatography
fingerprint
technique
establish
comprehensive
quality
assessment
system
from
different
regions.
Firstly,
optimal
conditions
ultrasonic‐assisted
extraction
two
organic
acids
were
optimized
through
response
surface
analysis.
A
liquid–solid
ratio
58:1,
methanol
67%,
time
50
min
determined.
Subsequently,
HPLC‐DAD
methods
quantification
established,
demonstrating
good
linearity,
repeatability,
recovery,
stability
quantitative
analysis
samples.
In
addition,
chemical
fingerprints
12
batches
samples
successfully
identifying
19
common
peaks.
more
reliable
was
provided
by
combining
similarity
analysis,
hierarchical
clustering
principal
component
(PCA),
orthogonal
partial
least
squares
discriminant
(OPLS‐DA)
other
chemometric
methods.
approach
significant
potential
in
industrial
scientific
applications
establishing
evaluation
standard
Herba,
facilitating
standardization
this
herb.
Язык: Английский
Comparison of Polysaccharide Profiles of Different Seaweeds Based on Ion Chromatography and Ultrahigh‐Performance Liquid Chromatography
Separation Science Plus,
Год журнала:
2024,
Номер
7(9)
Опубликована: Июль 23, 2024
ABSTRACT
In
traditional
Chinese
medicine
research,
seaweeds
used
for
drug
and
health
food
development
mainly
refer
to
Sargassum
pallidum
(Turn.)
C.
Ag.
(SP)
fusiforme
(Harv.)
Setch.
(SF).
the
Pharmacopoeia,
polysaccharides
are
main
quality
control
indicators.
The
total
sugar
content
is
determined
by
anthrone–sulfuric
acid
method
evaluate
of
seaweeds.
However,
this
cannot
reflect
structural
characteristics
seaweed
distinguish
among
different
varieties.
Given
this,
comprehensively
seaweeds,
study
conducted
a
thorough
analysis
primary
structure
in
11
batches
SF
7
SP.
neutral
was
colorimetric
method,
weight
average
molecular
(
M
w)
high‐performance
size
exclusion
chromatography
coupled
with
multi‐angle
laser
light
scattering
refractive
index
detector
sulfate
ion
monosaccharide
composition
ultrahigh‐performance
liquid
while
also
using
professional
software
statistical
similarity
analysis.
results
showed
significant
differences
p
<
0.05)
w
between
S.
polysaccharides,
which
can
be
fingerprint
analyzed
chemometric
methods,
it
found
that
fucose
glucose
could
serve
as
differential
markers
This
further
deepens
understanding
more
evaluates
Язык: Английский