Опубликована: Янв. 1, 2024
Язык: Английский
Molecular Plant, Год журнала: 2023, Номер 16(12), С. 1893 - 1910
Опубликована: Окт. 28, 2023
Язык: Английский
Процитировано
26
Plant Biotechnology Journal, Год журнала: 2024, Номер 22(10), С. 2660 - 2671
Опубликована: Май 22, 2024
Sugarcane (Saccharum spp. hybrid) is a prime feedstock for commercial production of biofuel and table sugar. Optimizing canopy architecture improved light capture has great potential elevating biomass yield. LIGULELESS1 (LG1) involved in leaf ligule auricle development grasses. Here, we report CRISPR/Cas9-mediated co-mutagenesis up to 40 copies/alleles the putative LG1 highly polyploid sugarcane (2n = 100-120, x 10-12). Next generation sequencing revealed co-editing frequencies 7.4%-100% reads 16 78 transgenic lines. mutations resulted tuneable angle phenotype that became more upright as frequency increased. Three lines with loss function ~12%, ~53% ~95% lg1 were selected following randomized greenhouse trial grown replicated, multi-row field plots. The co-edited stably maintained vegetative progenies extent remained constant tested L26 L35. confirmed absence off targets. inclination corresponded transmission into tiller number. Line L35 displaying ~12% NGS exhibited an 18% increase dry yield supported by 56% decrease angle, 31% number, 25% internode scalable allows fine-tuning enabling selection ideotype
Язык: Английский
Процитировано
8
Frontiers in Genome Editing, Год журнала: 2023, Номер 5
Опубликована: Июль 20, 2023
Increased understanding of plant genetics and the development powerful easier-to-use gene editing tools over past century have revolutionized humankind's ability to deliver precise genotypes in crops. Plant transformation techniques are well developed for making transgenic varieties certain crops model organisms, yet reagent delivery regeneration remain key bottlenecks applying technology most Typical protocols produce transgenic, genetically modified (GM) rely on transgenes, chemical selection, tissue culture. make edited (GE) also use even though these may be undesirable final crop product. In some crops, transgenes routinely segregated away during meiosis by performing crosses, thus only a minor concern. other particularly those propagated vegetatively, complex hybrids, or with long generation times, such crosses impractical impossible. This review highlights diverse strategies CRISPR/Cas reagents regenerable cells recover plants without unwanted integration transgenes. Some examples include delivering DNA-free as ribonucleoproteins mRNA, relying expression from non-integrated DNA, using novel mechanisms viruses nanoparticles, unconventional selection methods avoid and/or avoiding culture altogether. These advancing rapidly already enabling scientists precision CRISPR tools.
Язык: Английский
Процитировано
19
The Plant Journal, Год журнала: 2025, Номер 121(5)
Опубликована: Март 1, 2025
Sugarcane, which provides 80% of global table sugar and 40% biofuel, presents unique breeding challenges due to its highly polyploid, heterozygous, frequently aneuploid genome. Significant progress has been made in developing genetic resources, including the recently completed reference genome sugarcane cultivar R570 pan-genomic resources from sorghum, a closely related diploid species. Biotechnological approaches RNA interference (RNAi), overexpression transgenes, gene editing technologies offer promising avenues for accelerating improvement. These methods have successfully targeted genes involved important traits such as sucrose accumulation, lignin biosynthesis, biomass oil stress response. One main transformation methods-biolistic transfer or Agrobacterium-mediated transformation-coupled with efficient tissue culture protocols, is typically used implementing these biotechnology approaches. Emerging show promise overcoming current limitations. The use morphogenic can help address genotype constraints improve efficiency. Tissue culture-free technologies, spray-induced silencing, virus-induced editing, potential functional genomics studies. Additionally, novel base prime orthogonal synthetic transcription factors, directed evolution present opportunities enhancing traits. advances collectively aim sugarcane's efficiency crop both biofuel production. This review aims discuss methodologies, focus on RNAi approaches, how be inform targets, future improvements applications.
Язык: Английский
Процитировано
0
Frontiers in Genetics, Год журнала: 2024, Номер 15
Опубликована: Апрель 19, 2024
The cultivated strawberry, Fragaria ×ananassa , is a recently domesticated fruit species of economic interest worldwide. As such, there significant in continuous varietal improvement. Genomics-assisted improvement, including the use DNA markers and genomic selection have facilitated improvements numerous key traits during strawberry breeding. CRISPR/Cas-mediated genome editing allows targeted mutations precision nucleotide substitutions target genome, revolutionizing functional genomics crop Genome beginning to gain traction more challenging polyploid crops, allo-octoploid strawberry. release high-quality reference genomes comprehensive subgenome-specific genotyping gene expression profiling data octoploid will lead surge trait discovery modification by using CRISPR/Cas. has already been successfully applied for several genes, anthocyanin content, firmness tolerance post-harvest disease. However, reports on many other important breeding characteristics associated with quality production are still lacking, indicating need streamlined approaches tools . In this review, we present an overview latest advancements knowledge efforts involving CRISPR/Cas enhancement varieties. Furthermore, explore potential applications technology improving Rosaceous plant species.
Язык: Английский
Процитировано
4
Frontiers in Plant Science, Год журнала: 2025, Номер 15
Опубликована: Янв. 7, 2025
Rapeseed (Brassica napus L.) is known for its high-quality seed oil and protein content. However, use in animal feed restricted due to antinutritional factors present the seedcake, with sinapine being one of main compounds that reduces palatability. Attempts develop rapeseed germplasm lower levels through traditional breeding methods have shown limited progress. Genetic transformation could create new genotypes reduced by silencing key genes involved biosynthesis, though these often result transgenic or genetically modified plants. The recent development CRISPR-Cas technology provides a precise efficient approach crop improvement, potential generate transgene-free mutants. In this study, we targeted BnaX.SGT.a knockout using editing. By utilizing our newly established protoplast regeneration transfection protocol rapeseed, demonstrated DNA-free CRISPR editing via protoplast-based ribonucleoprotein (RNP) delivery was highly effective. We achieved successful paralogues, an average mutation efficiency over 30%. Sequencing results revealed variety types, from 1 bp insertions 10 deletions, most mutants exhibiting frameshift mutations led premature stop codons. displayed no visible phenotypic differences growth patterns flowering compared wild type. Importantly, content significantly all T2 generation analysed, while weight remained comparable between
Язык: Английский
Процитировано
0
Sugar Tech, Год журнала: 2025, Номер unknown
Опубликована: Март 13, 2025
Язык: Английский
Процитировано
0
BMC Plant Biology, Год журнала: 2025, Номер 25(1)
Опубликована: Апрель 1, 2025
Wheat is a major global crop, and increasing its productivity essential to meet the growing population demand. However limited water resources primary constraint. This study aimed identify genetic factors associated with drought tolerance using diverse panel of 287 wheat genotypes evaluated under well-watered drought-stressed conditions. Water Use Efficiency (WUE) Grain Yield (GY), along indices, were assessed. A genome-wide association (GWAS) 26,814 high-density SNP markers identified loci linked these traits, 768 SNPs showing significant associations. Additionally, genomic selection (GS) was performed rrBLUP model predict trait performance across environments. Among measured traits at -log10 (P) ≥ 3, 81 mapped higher threshold 4, indicating pleiotropic QTL-by-environment interaction effects. Several novel known genes, previously reported have functions related biotic abiotic stresses response SNPs. indices evaluated, stress index (STI), geometric mean (GMP), (TOL) most reliable indicators for selecting stable, high-yielding control The same three exhibited high prediction values severe (SS) condition. Five as promising candidates breeding programs based on their superior tolerance, grain yield, nutritional value. provides valuable insights into basis in wheat, highlighting key regions improved use efficiency yield stability. findings contribute development drought-tolerant varieties optimized utilization achieve increased per unit levels, ultimately contributing sustainable agriculture food security.
Язык: Английский
Процитировано
0
Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 27 - 59
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Frontiers in Plant Science, Год журнала: 2023, Номер 14
Опубликована: Июнь 20, 2023
CRISPR-mediated genome editing has emerged as a powerful tool for creating targeted mutations in the various applications, including studying gene functions, engineering resilience against biotic and abiotic stresses, increasing yield quality. However, its utilization is limited to model crops which well-annotated sequences are available. Many of dietary economic importance, such wheat, cotton, rapeseed-mustard, potato, polyploids with complex genomes. Therefore, progress these been hampered due complexity. Excellent work conducted on some species Brassica improvement through editing. Although excellent editing, polyploid crops, U's triangle species, holds numerous implications improving other crops. In this review, we summarize key examples from done discuss important considerations deploying more efficiently improvement.
Язык: Английский
Процитировано
10