CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane

Frontiers in Plant Science, Год журнала: 2024, Номер 14

Опубликована: Янв. 8, 2024

Gene editing technologies have opened up the possibility of manipulating genome any organism in a predicted way. CRISPR technology is most used tool and, agriculture, it has allowed expansion possibilities plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA prime and nucleic acid probing detection. This mostly depends on

Язык: Английский

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CRISPR/Cas genome editing in plants: mechanisms, applications, and overcoming bottlenecks

Functional & Integrative Genomics, Год журнала: 2024, Номер 24(2)

Опубликована: Март 5, 2024

Язык: Английский

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Utilizing CRISPR-based genetic modification for precise control of seed dormancy: progress, obstacles, and potential directions

Molecular Biology Reports, Год журнала: 2025, Номер 52(1)

Опубликована: Фев. 5, 2025

Язык: Английский

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Modified carbon dot‐mediated transient transformation for genomic and epigenomic studies in wheat

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Фев. 19, 2025

Summary Genotype restriction poses a significant bottleneck to stable transformation in the vast majority of plant species, thereby severely impeding advancement bioengineering, particularly for crops. Nanoparticles (NPs) can serve as effective carriers transient delivery nucleic acids, facilitating gene overexpression or silencing plants genotype‐independent manner. However, applications NP‐mediated systems comprehensive genomic studies remained underexplored plants, especially crops that face challenges genetic transformation. Consequently, there is an urgent need efficient capable generating whole seedlings with uniformly transformed acids. We have developed straightforward and modified carbon dot (MCD)‐mediated system delivering DNA plasmids into seeds wheat, which also applicable other species. This facilitates generation contain transferred plasmids. Furthermore, our study demonstrates this serves excellent platform conducting functional including validation functions, protein interactions regulation, omics studies, genome editing. significantly enhances research any Thus, provides first time evidence new MCDs genomics epigenomic bioengineering potentially leading improvement desirable agronomic traits

Язык: Английский

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Generation of transgene-free canker-resistant Citrus sinensis cv. Hamlin in the T0 generation through Cas12a/CBE co-editing

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Март 26, 2024

Citrus canker disease affects citrus production. This is caused by Xanthomonas citri subsp. (Xcc). Previous studies confirmed that during Xcc infection, PthA4, a transcriptional activator like effector (TALE), translocated from the pathogen to host plant cells. PthA4 binds binding elements (EBEs) in promoter region of susceptibility gene LOB1 (EBE -LOBP) activate its expression and subsequently cause symptoms. Previously, Cas12a/CBE co-editing method was employed disrupt EBE -LOBP pummelo, which highly homozygous. However, most commercial cultivars are heterozygous hybrids more difficult generate homozygous/biallelic mutants. Here, we edit Hamlin ( sinensis ), hybrid cultivar grown worldwide. Binary vector GFP-p1380N-ttLbCas12a:LOBP1-mPBE:ALS2:ALS1 constructed shown be functional via Xcc-facilitated agroinfiltration leaves. construct allows selection transgene-free regenerants GFP, edits ALS chlorsulfuron-resistant as marker for genome editing resulting transient T-DNA nCas9-mPBE:ALS2:ALS1, gene(s) interest (i.e., this study) through ttLbCas12a, thus creating citrus. Totally, 77 plantlets were produced. Among them, 8 transgenic plants (#Ham GFP 1 - #Ham 8), 4 NoGFP 4), rest wild type. plantlets, three lines 1, 2 3) contained biallelic mutations pthA4 , one line 4) had homozygous . We achieved 5.2% mutation efficiency –LOBP C. cv. Hamlin, compared 1.9% pummelo previous study. Importantly, four 3 survived resistant against canker. Taken together, has been successfully used canker‐resistant T0 generation biallelic/homozygous LOB1.

Язык: Английский

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Genetic Transformation of Forest Trees and Its Research Advances in Stress Tolerance

Forests, Год журнала: 2024, Номер 15(3), С. 441 - 441

Опубликована: Фев. 26, 2024

Forests represent a vital natural resource and play crucial role in climate regulation maintaining biodiversity. However, the growth development of forest trees are increasingly challenged by rising environmental pressures, particularly detrimental abiotic stressors. To address these challenges, genetic transformation technologies have emerged as effective solutions. Despite various difficulties for trees, including prolonged life cycles, diversity, interspecies variations, complex regeneration systems, significant research progress has been achieved tree gene editing, transgenic technology, methods delivering exogenous molecules. These potential to enhance quality, increase productivity, improve resistance stress. This review provides an overview main receptors transformation. Additionally, we summarize several novel techniques, such nanoparticle-mediated transformation, advanced editing delivery carriers, non-genetically modified protein function interference through peptide aptamer. Notably, also place emphasis on referable genes from common crops, together with their improving stress responses. Through this research, aspire achieve sustainable utilization conservation resources, thereby providing substantial support future livelihoods economic development.

Язык: Английский

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Recent advances of CRISPR-based genome editing for enhancing staple crops

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Сен. 23, 2024

An increasing population, climate change, and diminishing natural resources present severe threats to global food security, with traditional breeding genetic engineering methods often falling short in addressing these rapidly evolving challenges. CRISPR/Cas systems have emerged as revolutionary tools for precise modifications crops, offering significant advancements resilience, yield, nutritional value, particularly staple crops like rice maize. This review highlights the transformative potential of technology, emphasizing recent innovations such prime base editing, development novel CRISPR-associated proteins, which significantly improved specificity, efficiency, scope genome editing agriculture. These enable targeted that enhance tolerance abiotic stresses well biotic stresses. Additionally, plays a crucial role improving crop yield quality by enhancing photosynthetic nutrient uptake, resistance lodging, while also taste, texture, shelf life, content through biofortification. Despite challenges off-target effects, need more efficient delivery methods, ethical regulatory concerns, underscores importance security sustainability It calls continued research integration CRISPR other emerging technologies nanotechnology, synthetic biology, machine learning fully realize its developing resilient, productive, sustainable agricultural systems.

Язык: Английский

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Genetic and molecular insights into tiller development and approaches for crop yield improvement

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Март 27, 2025

Tiller development is a critical factor in boosting agricultural productivity and securing global food security. This review offers comprehensive analysis of recent advancements enhancing crop yield through extensive research on tiller development, utilizing multi-faceted approach that includes quantitative trait loci (QTL) mapping, association studies, transcriptome across various crops. Extensive investigations have revealed complex genetic, molecular, environmental interactions influence this pivotal determinant. QTL mapping has pinpointed specific genomic regions associated with while genome-wide studies (GWAS) provided deeper insights into natural genetic variations within populations. Additionally, analyses offered dynamic view gene expression, shedding light molecular regulatory mechanisms govern tillering. The integration these multi-omics approaches enabled holistic understanding the process, identifying crucial expression patterns are key to optimizing Key genes such as TaMAX1, TaMOC1 , TN1 wheat, ZmTB1, ZmD14, ZmMOC1 maize, along MAX1 -like genes, OsMAX1, OsHAM2 rice been highlighted. Similar sugarcane identified like SoMAX2, SoMAX3 SoMAX4-1, SoMAX4-2 SoTB1 which regulate bud outgrowth Including involving hormonal control integrates pathway auxins, gibberellins, cytokinins, coordinate plant responses internal external stimuli “These” discoveries essential for breeding engineering strategies aimed at developing varieties favorable tillering traits, ultimately potential.

Язык: Английский

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Genome Editing: An Approach to Engineer Crops to Withstand Climate Change and Sustain Food Security

Опубликована: Янв. 1, 2025

Язык: Английский

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Application and Prospects of DNA-free Genome Modifications in Crop Plants through Genome Editing

CABI eBooks, Год журнала: 2025, Номер unknown, С. 146 - 165

Опубликована: Март 21, 2025

Язык: Английский

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