Spiroplasma endosymbiont reduction of host lipid synthesis and Stomoxyn-like peptide contribute to trypanosome resistance in the tsetse fly Glossina fuscipes
PLoS Pathogens,
Год журнала:
2025,
Номер
21(1), С. e1012692 - e1012692
Опубликована: Янв. 31, 2025
Tsetse
flies
(Glossina
spp.)
vector
African
trypanosomes
that
cause
devastating
diseases
in
humans
and
domestic
animals.
Within
the
Glossina
genus,
species
Palpalis
subgroup
exhibit
greater
resistance
to
trypanosome
infections
compared
those
Morsitans
subgroup.
Varying
microbiota
composition
species-specific
genetic
traits
can
significantly
influence
efficiency
of
parasite
transmission.
Notably,
with
endosymbiotic
bacterium
Spiroplasma
have
been
documented
several
species,
including
fuscipes
(Gff).
While
Gff
are
known
hinder
transmission,
underlying
mechanisms
remain
unknown.
To
investigate
Spiroplasma-mediated
factors
affecting
competence,
we
conducted
high-throughput
RNA
sequencing
gut
tissue
along
functional
assays.
Our
findings
reveal
elevated
oxidative
stress
environment
presence
Spiroplasma,
evidenced
by
increased
expression
nitric
oxide
synthase,
which
catalyzes
production
trypanocidal
oxide.
Additionally,
observed
impaired
lipid
biosynthesis
leading
a
reduction
this
important
class
nutrients
essential
for
host
physiologies.
In
contrast,
Gff's
midgut
upregulated
various
immunity-related
genes,
small
peptide,
Stomoxyn-like,
homologous
Stomoxyn
first
discovered
stable
fly,
Stomoxys
calcitrans.
We
Stomoxyn-like
locus
is
exclusive
genomes
tsetse
species.
GffStomoxyn
constitutively
expressed
cardia
(proventriculus)
synthetic
exhibits
potent
activity
against
Escherichia
coli
bloodstream
form
Trypanosoma
brucei
parasites,
while
showing
no
effect
insect
stage
procyclic
forms
or
tsetse's
commensal
endosymbiont
Sodalis
vitro.
Reducing
levels
infection
prevalence,
indicating
its
potential
role
vivo.
Collectively,
our
results
suggest
enhanced
Spiroplasma-infected
may
be
due
reduced
availability
necessary
metabolic
maintenance.
Furthermore,
could
play
crucial
initial
immune
response(s)
mammalian
parasites
early
process
prevent
colonization.
discuss
molecular
characteristics
GffStomoxyn,
spatial
temporal
regulation
microbicidal
Trypanosome
parasites.
reinforce
nutritional
influences
on
physiology
host-pathogen
dynamics.
Язык: Английский
Octanoic acid kills Lucilia sericata (Diptera: Calliphoridae) by affecting two major defence systems: cuticular free fatty acids and immunocompetent cells
Journal of Invertebrate Pathology,
Год журнала:
2024,
Номер
206, С. 108165 - 108165
Опубликована: Июль 8, 2024
Язык: Английский
The flavouring agent, 2-octenoic acid kills Galleria mellonella (Lepidoptera: Pyralidae) by affecting their immunocompetent cells and cuticular FFA profiles
Journal of Insect Physiology,
Год журнала:
2025,
Номер
161, С. 104779 - 104779
Опубликована: Фев. 11, 2025
Язык: Английский
Exploring the Insecticidal and Antimalarial Potentials of Pseudomonas aeruginosa Azurin: A Promising Alternative for Integrated Malaria Control
Microbial Pathogenesis,
Год журнала:
2025,
Номер
206, С. 107773 - 107773
Опубликована: Июнь 3, 2025
Язык: Английский
Spiroplasma endosymbiont reduction of host lipid synthesis and Stomoxyn-like peptide contribute to trypanosome resistance in the tsetse fly Glossina fuscipes
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 24, 2024
Abstract
Tsetse
flies
(
Glossina
spp.)
vector
African
trypanosomes
that
cause
devastating
diseases
in
humans
and
domestic
animals.
Within
the
genus,
species
Palpalis
subgroup
exhibit
greater
resistance
to
trypanosome
infections
compared
those
Morsitans
subgroup.
Varying
microbiota
composition
species-specific
genetic
traits
can
significantly
influence
efficiency
of
parasite
transmission.
Notably,
with
endosymbiotic
bacterium
Spiroplasma
have
been
documented
several
species,
including
fuscipes
Gff
).
While
are
known
hinder
transmission,
underlying
mechanisms
remain
unknown.
To
investigate
Spiroplasma-
mediated
factors
affecting
competence,
we
conducted
high-throughput
RNA
sequencing
midgut
tissue
along
functional
assays.
Our
findings
reveal
elevated
oxidative
stress
environment
presence
,
evidenced
by
increased
expression
nitric
oxide
synthase,
which
catalyzes
production
trypanocidal
oxide.
Additionally,
observed
impaired
lipid
biosynthesis
leading
a
reduction
this
important
class
nutrients
essential
for
host
physiologies.
In
contrast,
Gff’s
upregulated
various
immunity-related
genes,
small
peptide,
Stomoxyn-like
homologous
Stomoxyns
first
discovered
stable
fly
Stomoxys
calcitrans
.
We
locus
is
exclusive
genomes
tsetse
species.
Stomoxyn
constitutively
expressed
cardia
(proventriculus)
synthetic
exhibits
potent
activity
against
Escherichia
coli
bloodstream
form
Trypanosoma
brucei
parasites,
while
showing
no
effect
insect
stage
procyclic
forms
or
tsetse’s
commensal
endosymbiont
Sodalis
vitro
Reducing
levels
infection
prevalence,
indicating
its
potential
role
vivo
Collectively,
our
results
suggest
enhanced
-infected
may
be
due
reduced
availability
necessary
metabolic
maintenance.
Furthermore,
could
play
crucial
initial
immune
response(s)
mammalian
parasites
early
process
prevent
gut
colonization.
discuss
molecular
characteristics
Stomoxyn,
spatial
temporal
regulation
microbicidal
Trypanosome
parasites.
reinforce
nutritional
influences
on
physiology
host-pathogen
dynamics.
Author
Summary
The
fly,
)
high
public
health
relevance.
strong
innate
trypanosomes,
especially
when
infected
This
study
investigated
how
inside
enables
them
resistant
infection.
indicate
alterations
metabolism
triglycerides,
suggesting
barrier
limits
viability
parasite.
addition,
stomoxyn,
exclusively
related
shown
has
antibacterial
antitrypanosomal
properties
lowering
correlates
prevalence.
strategies
increase
prevalence
enhance
stomoxyn
through
paratransgenic
approaches
promising
avenues
reducing
trypanosomiasis
Язык: Английский