Dynamic encoding of temperature in the central circadian circuit coordinates physiological activities
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Апрель 2, 2024
Abstract
The
circadian
clock
regulates
animal
physiological
activities.
How
temperature
reorganizes
circadian-dependent
activities
remains
elusive.
Here,
using
in-vivo
two-photon
imaging
with
the
control
device,
we
investigated
response
of
Drosophila
central
circuit
to
variation
and
identified
that
DN1as
serves
as
most
sensitive
temperature-sensing
neurons.
gate
DN1a’s
diurnal
response.
Trans-synaptic
tracing,
connectome
analysis,
functional
data
reveal
bidirectionally
targets
two
neuronal
subsets:
activity-related
E
cells
sleep-promoting
DN3s.
Specifically,
behavioral
demonstrate
DN1a-E
cell
modulates
evening
locomotion
peak
in
cold
temperature,
while
DN1a-DN3
controls
warm
temperature-induced
nocturnal
sleep
reduction.
Our
findings
systematically
comprehensively
illustrate
how
dynamically
integrates
light
signals
effectively
coordinate
wakefulness
at
different
times
day,
shedding
on
conserved
neural
mechanisms
underlying
temperature-regulated
physiology
animals.
Язык: Английский
Biotechnology-enhanced genetic controls of the global pest Drosophila suzukii
Trends in biotechnology,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 1, 2024
Язык: Английский
Promoter engineering with programmable upstream activating sequences in Aspergillus Niger cell factory
Microbial Cell Factories,
Год журнала:
2025,
Номер
24(1)
Опубликована: Янв. 15, 2025
Язык: Английский
Taking flight, the use of Drosophila melanogaster for neuroscience research in Uruguay
Neuroscience,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 1, 2025
Язык: Английский
Redesigning and rethinking genetic circuits: the potential of transcriptional rewiring in filamentous fungi
Current Opinion in Biotechnology,
Год журнала:
2025,
Номер
93, С. 103301 - 103301
Опубликована: Апрель 4, 2025
Язык: Английский
Improved whole-mount immunofluorescence protocol for consistent and robust labeling of adult Drosophila melanogaster adipose tissue
Biology Open,
Год журнала:
2024,
Номер
13(8)
Опубликована: Июль 23, 2024
Energy
storage
and
endocrine
functions
of
the
Drosophila
fat
body
make
it
an
excellent
model
for
elucidating
mechanisms
that
underlie
physiological
pathophysiological
organismal
metabolism.
Combined
with
Drosophila's
robust
genetic
immunofluorescence
microscopy
toolkits,
studies
function
are
ripe
cell
biological
analysis.
Unlike
larval
body,
which
is
easily
removed
as
a
single,
cohesive
sheet
tissue,
isolating
intact
adult
proves
to
be
more
challenging,
thus
hindering
consistent
labeling
even
within
single
piece
adipose
tissue.
Here,
we
describe
improved
approach
handling
abdomens
ensures
full
access
solutions
generally
used
in
protocols.
In
addition,
assess
quality
fluorescence
reporter
expression
antibody
immunoreactivity
response
variations
fixative
type,
fixation
incubation
time,
detergent
cellular
permeabilization.
Overall,
provide
several
recommendations
steps
whole-mount
staining
protocol
results
body.
Язык: Английский
Transposon and Transgene Tribulations in Mosquitoes: A Perspective of piRNA Proportions
DNA,
Год журнала:
2024,
Номер
4(2), С. 104 - 128
Опубликована: Март 30, 2024
Mosquitoes,
like
Drosophila,
are
dipterans,
the
order
of
“true
flies”
characterized
by
a
single
set
two
wings.
Drosophila
prime
model
organisms
for
biomedical
research,
while
mosquito
researchers
struggle
to
establish
robust
molecular
biology
in
these
that
arguably
most
dangerous
vectors
human
pathogens.
Both
insects
utilize
RNA
interference
(RNAi)
pathway
generate
small
RNAs
silence
transposons
and
viruses,
yet
details
emerging
several
RNAi
features
unique
each
insect
family,
such
as
how
culicine
mosquitoes
have
evolved
extreme
genomic
feature
differences
connected
their
features.
A
major
technical
difference
genetic
studies
is
generating
stable
transgenic
animals
routine
but
still
variable
stability
mosquitoes,
despite
DNA-editing
advances.
By
comparing
contrasting
pathways
this
review
we
propose
hypothesis
transgene
DNAs
possibly
more
intensely
targeted
chromatin
regulatory
than
Drosophila.
We
latest
findings
on
pathways,
which
much
less
well
understood
speculate
deeper
study
into
modulate
viruses
with
Piwi-interacting
(piRNAs)
will
yield
clues
improving
DNA
expression
mosquitoes.
Язык: Английский
Improved whole-mount immunofluorescence protocol for consistent and robust labeling of adultDrosophila melanogasteradipose tissue
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 15, 2024
ABSTRACT
Energy
storage
and
endocrine
functions
of
the
Drosophila
fat
body
make
it
an
excellent
model
for
elucidating
mechanisms
that
underlie
physiological
pathophysiological
organismal
metabolism.
Combined
with
Drosophila’s
robust
genetic
immunofluorescence
microscopy
toolkits,
studies
function
are
ripe
cell
biological
analysis.
Unlike
larval
body,
which
is
easily
removed
as
a
single,
cohesive
sheet
tissue,
isolating
intact
adult
proves
to
be
more
challenging,
thus
hindering
consistent
labeling
even
within
single
piece
adipose
tissue.
Here,
we
describe
improved
approach
handling
abdomens
ensures
full
access
solutions
generally
used
in
protocols.
In
addition,
assess
quality
fluorescence
reporter
expression
antibody
immunoreactivity
response
variations
fixative
type,
fixation
incubation
time,
detergent
cellular
permeabilization.
Overall,
provide
several
recommendations
steps
whole
mount
staining
protocol
results
body.
SUMMARY
STATEMENT
Optimization
via
modifications
tissue
handling,
fixation,
permeabilization
immunolabeling
protocol.
Язык: Английский