Subchronic Exposure to Polystyrene Nanoplastics Disrupts Placental Development and Calcium Homeostasis: Insights from In Vivo and In Vitro Models

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Апрель 2, 2025

Nanoplastics have recently emerged as persistent pollutants of global concern that pose substantial risks to human health. However, the long-term adverse effects nanoplastics on female reproductive system remain unclear. Polystyrene (PS-NPs; 50 nm diameter) were selected representative nanosized plastic particles investigate potential subchronic prenatal and gestational exposure via drinking water placental development in ICR (CD-1) mice. Maternal 10 mg/L PS-NPs induced an increase fetal resorption rate significantly increased weight. Further observation morphology showed led aberrant structure damaged trophoblast cells. At cellular level, promoted proliferation, migration, invasion HTR-8/SVneo Mechanistically, transcriptomic proteomic analyses revealed triggered calcium disturbances upregulated Stam2 expression STAM2 by mediates disruption trophoblastic homeostasis regulates cell functions disturbing lysosomal degradation channel protein IP3R3 promoting intracellular inflow increasing level TRPV6 Therefore, our results indicated dyshomeostasis is main mechanism which induces abnormal development. These findings reveal a link between damage elucidate underlying molecular mechanism, providing evidence for environmental triggers pregnancy highlighting risk products pregnant women.

Язык: Английский

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Nutrition and Metabolism: Foundations for Animal Growth, Development, Reproduction, and Health

Advances in experimental medicine and biology, Год журнала: 2021, Номер unknown, С. 1 - 24

Опубликована: Ноя. 22, 2021

Язык: Английский

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A head start: The relationship of placental factors to craniofacial and brain development

Developmental Dynamics, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

Abstract In recent years, the importance of placental function for fetal neurodevelopment has become increasingly studied. This field, known as neuroplacentology, greatly expanded possible etiologies neurodevelopmental disorders by exploring influence on brain development. It is also well‐established that development influenced craniofacial morphogenesis. However, there less focus impact placenta Recent research suggests functional nutrients and hormones skeletal growth, such prolactin, growth hormone, insulin‐like factor 1, vitamin D, sulfate, calcium, impacting both Therefore, interactions between likely morphology head a whole. review discusses role hormone production nutrient delivery in head—defined tissue together—expanding more established to include skull (or cranium) face.

Язык: Английский

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Phosphate, calcium, and vitamin D signaling, transport, and metabolism in the endometria of cyclic ewes

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Год журнала: 2023, Номер 14(1)

Опубликована: Янв. 12, 2023

Recent evidence suggests important roles for progesterone (P4) and interferon tau in the regulation of calcium, phosphate, vitamin D signaling uteri pregnant sheep. However, effects P4 estradiol (E2), with respect to expression their receptors PGR ESR1, respectively, uterine epithelia on mineral during estrous cycle has not been investigated. Estrous cycles mature Suffolk ewes were synchronized, prostaglandin F2α was administered, observed estrus (designated as Day 0) presence vasectomized rams. On Days 1, 9, or 14 cycle, hysterectomies performed.25-hydroxyvitamin more abundant plasma from than 1 (P < 0.05). Expression fibroblast growth factor receptor 2 (FGFR2), a disintegrin metalloprotease 17 (ADAM17), parathyroid hormone-related protein (PTHrP) mRNAs greater endometria 9 compared 0.01). Similarly, transient potential cation channel subfamily V member 6 (TRPV6) mRNA ATPase membrane Ca2+ transporting 4 (ATP2B4) S100 calcium binding G (S100G) In contrast, endometrial (VDR) lower klotho (KL) 0.05) cytochrome P450 family 24 A (CYP24) 0.01) 9. ADAM17, FGF23, CYP2R1, CYP27B1, KL, VDR proteins immunolocalized myometrium, blood vessels, luminal (LE), superficial glandular (sGE), (GE) epithelia. S100A9 weakly expressed LE, sGE, GE. Immunoreactivity CYP2R1 KL LE sGE less 14. S100G exclusively by GE, immunoreactive S100A12 localized stromal cells stratum spongiosum but epithelial cells.Collectively, these results implicate E2, P4, cyclic ewes.

Язык: Английский

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Relationship of calcium and phosphorus content with indicators of reproductive ability in cattle

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology, Год журнала: 2024, Номер 26(113), С. 184 - 188

Опубликована: Фев. 24, 2024

The development of modern animal husbandry is quite an urgent issue today, because the consumption dairy and meat products growing rapidly. Factors affecting reproducibility play a key role in this issue. aim work was to investigate dependence content calcium phosphorus blood serum with indicators reproductive ability cows. A spectrophotometer used study content. correlation insemination index established, which moderate positive r = 0.494 (Р ≤ 0.001), service period – average 0.643 0.001) days 1st heat strong 0.862 0.001). Phosphorus has corresponding 0.599 had smallest correlation, 0.314 duration mating very 0.909 Based on obtained data, we can testify about reproducibility, such as index, heat. What gives reason for further research establishment interdependence reproducible during its correction from side influence elements that have these indicators.

Язык: Английский

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Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling

Biology of Reproduction, Год журнала: 2022, Номер 106(5), С. 888 - 899

Опубликована: Фев. 3, 2022

Abstract Given recent reports of expression postnatal mineral transport regulators at the maternal–conceptus interface during peri-implantation period, this study tested hypothesis that progesterone (P4) and interferon tau (IFNT) regulate phosphate, calcium, vitamin D signaling in ovine endometrium. Mature Rambouillet ewes (n = 24) were surgically fitted with intrauterine catheters on day 7 estrous cycle. Ewes received daily intramuscular injections 50 mg P4 corn oil vehicle 75 receptor antagonist (RU486) from days 8 to 15, twice-daily either control proteins (CX) or IFNT (25 μg/uterine horn/day) 11 15 resulting four treatment groups: + CX; IFNT; RU486 IFNT. On 16, hysterectomized. CX treated had lower concentrations 25 (OH) plasma than (P &lt; 0.05). Endometria greater FGF23 0.01), S100A9 0.05), S100A12 0.05) mRNAs ADAM10 mRNA 0.01) proteins. Expression was endometria (hormone × protein interaction, P The S100G compared 0.05; hormone 0.01). These data implicate regulation period pregnancy provide a platform for continued mechanistic investigations.

Язык: Английский

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Prevalence of 25-Hydroxyvitamin D (Vitamin D) Deficiency in a Group of Infertile Women from Baghdad City

Biochemistry Research International, Год журнала: 2023, Номер 2023, С. 1 - 6

Опубликована: Июнь 14, 2023

Background. Infertility is a common issue affecting large number of Iraqi women reproductive age. The relationship between vitamin D deficiency and infertility has previously drawn the attention gynecologists, an increasing testing been requested. Methods. 120 were enrolled in this study April 2019 2020. Patients divided into two groups comprising sixty complaining infertility, with other 60 being fertile as controls. All patients assessed for level. Results. In group, deficient, insufficient, sufficient level 28%, 23%, 48%, respectively (these numbers rounded to nearest whole digit, infertile group given that precision), whereas showed statistically significant ( $p$ value = 0.002) distribution levels 50%, 35%, 15% sufficient, respectively. Conclusions. Vitamin significantly deficient which suggests possible, positive impact if considered management female infertility. Further more participants highly recommended.

Язык: Английский

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Effects of exogenous progesterone on the expression of mineral regulatory molecules by ovine endometrium and placentomes

Biology of Reproduction, Год журнала: 2022, Номер 106(6), С. 1126 - 1142

Опубликована: Фев. 21, 2022

Abstract This study aimed to determine whether the acceleration of conceptus development induced by administration exogenous progesterone (P4) during preimplantation period pregnancy alters calcium, phosphate, and vitamin D signaling at maternal–conceptus interface. Suffolk ewes (n = 48) were mated fertile rams received daily intramuscular injections either corn oil (CO) vehicle or 25 mg in CO for first 8 days hysterectomized on Day 9 (CO, n 5; P4, 6), 12 9; 4) 125 14; 10) gestation. The expression S100A12 (P &lt; 0.05) fibroblast growth factor receptor (FGFR2) 0.01) messenger RNAs (mRNAs) was lower endometria from P4-treated 12. ADAM10 mRNA greater 125. 0.01), FGFR2 0.05), solute carrier (SLC)20A1 TRPV5 TRPV6 mRNAs greater, but KL placentomes There endometrial placentomal involved mineral metabolism transport twin compared singleton pregnancies. Further, placentomes. KL, FGF23, (VDR), S100A9, S100A12, S100G, CYP27B1 proteins immunolocalized Exogenous P4 early altered regulators indicating a novel effect

Язык: Английский

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Placental Element Content Assessed via Synchrotron-Based X-ray Fluorescence Microscopy Identifies Low Molybdenum Concentrations in Foetal Growth Restriction, Postdate Delivery and Stillbirth

Nutrients, Год журнала: 2024, Номер 16(15), С. 2549 - 2549

Опубликована: Авг. 3, 2024

Placental health and foetal development are dependent upon element homeostasis. Analytical techniques such as mass spectroscopy can provide quantitative data on concentrations in placental tissue but do not show spatial distribution or co-localisation of elements that may affect function. The present study used synchrotron-based X-ray fluorescence microscopy to elucidate content healthy pathological tissue. (XFM) beamline at the Australian Synchrotron was image trace metal 19 sections from term (n = 5, 37–39 weeks), growth-restricted 3, <32 weeks, birth weight <3rd centile), postdate 7, >41 completed stillbirth-complicated pregnancies 4, 37–40 weeks). Samples were cryo-sectioned freeze-dried. concentration fourteen detected all samples: arsenic, bromine, calcium, chlorine, copper, iron, molybdenum, phosphorous, potassium, rubidium, selenium, strontium, sulphur, zinc. zinc, strontium significantly increased stillbirth comparison healthy-term controls. Strontium, calcium found co-localise samples, correlated groups. Molybdenum decreased stillbirth, growth-restricted, samples (p < 0.0001). Synchrotron-based XFM reveals elemental within biological placenta, allowing for deposits have a role. Our pilot further indicates low molybdenum complicated by growth restriction, delivery, stillbirth.

Язык: Английский

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FGF23 level in poultry chicken, a systematic review and meta-analysis

Frontiers in Physiology, Год журнала: 2023, Номер 14

Опубликована: Окт. 16, 2023

Introduction: In vertebrates fibroblast growth factor 23 (FGF23) is a phosphate regulating hormone closely linked to calcium regulation by vitamin D and parathyroid (PTH). Although phosphorus, are important for poultry well-being, relatively little known about their levels of FGF23. Our objective was quantitatively estimate the blood FGF23 level in birds, examine its relationship diet other components homeostasis. Methods: A systematic search Agricola, Embase Medline identified 86 studies focused on from which 12 manuscripts reporting data 60 independent groups chickens were included analysis. Results: 256 pg/ml (Confidence interval (CI): 215, 297) broilers (39 datasets containing 435 birds), (CI: 178, 339) egg-laying hens (21 208 birds). did not correlate with dietary or D, plasma PTH. demonstrated trend positively strongly positive correlation D. Discussion: This study provides normative estimates birds new insights into

Язык: Английский

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