Regulation of microRNAs in Satellite Cell Renewal, Muscle Function, Sarcopenia and the Role of Exercise DOI Open Access
Stefania Fochi, Gaia Giuriato,

Tonia De Simone

и другие.

International Journal of Molecular Sciences, Год журнала: 2020, Номер 21(18), С. 6732 - 6732

Опубликована: Сен. 14, 2020

Sarcopenia refers to a condition of progressive loss skeletal muscle mass and function associated with higher risk falls fractures in older adults. Musculoskeletal aging leads reduced strength, affecting the quality life elderly people. In recent years, several studies contributed improve knowledge pathophysiological alterations that lead dysfunction; however, molecular mechanisms underlying sarcopenia are still not fully understood. Muscle development homeostasis require fine gene expression modulation by which microRNAs (miRNAs) play crucial role. miRNAs modulate key steps myogenesis including satellite cells renewal, plasticity, regeneration. Here, we provide an overview general aspects regeneration role plasticity special interest their adaptation exercise elderly.

Язык: Английский

Elevated CD47 is a hallmark of dysfunctional aged muscle stem cells that can be targeted to augment regeneration DOI Creative Commons
Ermelinda Porpiglia,

Thach Mai,

Peggy E. Kraft

и другие.

Cell stem cell, Год журнала: 2022, Номер 29(12), С. 1653 - 1668.e8

Опубликована: Ноя. 16, 2022

Язык: Английский

Процитировано

47

Advances and Challenges in Cell Biology for Cultured Meat DOI Creative Commons
Beatriz T. Martins,

Arthur Bister,

Richard G. J. Dohmen

и другие.

Annual Review of Animal Biosciences, Год журнала: 2023, Номер 12(1), С. 345 - 368

Опубликована: Ноя. 14, 2023

Cultured meat is an emerging biotechnology that aims to produce from animal cell culture, rather than the raising and slaughtering of livestock, on environmental welfare grounds. The detailed understanding accurate manipulation biology are critical design cultured bioprocesses. Recent years have seen significant interest in this field, with numerous scientific commercial breakthroughs. Nevertheless, these technologies remain at a nascent stage, myriad challenges remain, spanning entire bioprocess. From biological perspective, include identification suitable starting types, tuning proliferation differentiation conditions, optimization cell–biomaterial interactions create nutritious, enticing foods. Here, we discuss key advances outstanding meat, particular focus biology, argue solving remaining bottlenecks cost-effective, scalable fashion will require coordinated, concerted efforts. Success also solutions nonscientific challenges, including regulatory approval, consumer acceptance, market feasibility. However, if can be overcome, revolutionize our approach food.

Язык: Английский

Процитировано

35

The asymmetrical ESR1 signaling in muscle progenitor cells determines the progression of adolescent idiopathic scoliosis DOI Creative Commons
Xiexiang Shao, Xin Fu,

Jingfan Yang

и другие.

Cell Discovery, Год журнала: 2023, Номер 9(1)

Опубликована: Апрель 25, 2023

Adolescent Idiopathic Scoliosis (AIS) is a common pediatric skeletal disease highly occurred in females. The pathogenesis of AIS has not been fully elucidated. Here, we reveal that ESR1 (Estrogen Receptor 1) expression declines muscle stem/progenitor cells at the concave side patients. Furthermore, required for cell differentiation and disrupted signaling leads to defects. imbalance para-spinal muscles induces scoliosis mice, while reactivation by an FDA approved drug Raloxifene alleviates curve progression. This work reveals asymmetric inactivation one causes AIS. Reactivation could be new strategy treat

Язык: Английский

Процитировано

26

Perfusable adipose decellularized extracellular matrix biological scaffold co-recellularized with adipose-derived stem cells and L6 promotes functional skeletal muscle regeneration following volumetric muscle loss DOI
Wei Liang, Meng Han, Li Guan

и другие.

Biomaterials, Год журнала: 2024, Номер 307, С. 122529 - 122529

Опубликована: Март 11, 2024

Язык: Английский

Процитировано

12

Protocol for the three-dimensional analysis of rodent skeletal muscle DOI

Smrithi Karthikeyan,

Yoko Asakura, Mayank Verma

и другие.

STAR Protocols, Год журнала: 2025, Номер 6(1), С. 103549 - 103549

Опубликована: Янв. 10, 2025

Язык: Английский

Процитировано

2

IL-1β and TNF-α Modulation of Proliferated and Committed Myoblasts: IL-6 and COX-2-Derived Prostaglandins as Key Actors in the Mechanisms Involved DOI Creative Commons
Ángela M. Álvarez, Carlos DeOcesano-Pereira, Catarina Teixeira

и другие.

Cells, Год журнала: 2020, Номер 9(9), С. 2005 - 2005

Опубликована: Сен. 1, 2020

In this study, we investigated the effects and mechanisms of pro-inflammatory cytokines IL-1β TNF-α on proliferation commitment phases myoblast differentiation. C2C12 mouse cells were cultured to reach a proliferated or committed status incubated with these for evaluation cell proliferation, cyclooxygenase 2 (COX-2) expression, release prostaglandins (PGs) myokines, activation myogenic regulatory factors (MRFs). We found that inhibition IL-6 receptor reduced IL-1β- TNF-α-induced effect also involved COX-2-derived PGs. Both modulated myokines myostatin, irisin, osteonectin, IL-15. activity Pax7 in MyoD myogenin at both proliferative stages. Otherwise, increased only cells. Our data reveal key role PGs support link between MRFs. concluded induce similar initial stages muscle regeneration but critical differences their progression process, bringing new insights into inflammatory signalling skeletal regeneration.

Язык: Английский

Процитировано

57

Stem Cell and Macrophage Roles in Skeletal Muscle Regenerative Medicine DOI Open Access
Pasqualina Scala,

Laura Rehak,

Valentina Giudice

и другие.

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(19), С. 10867 - 10867

Опубликована: Окт. 8, 2021

In severe muscle injury, skeletal tissue structure and functionality can be repaired through the involvement of several cell types, such as stem cells, innate immune responses. However, exact mechanisms behind regeneration, homeostasis, plasticity are still under investigation, discovery pathways types involved in repair open way for novel therapeutic approaches, cell-based therapies involving cells peripheral blood mononucleate cells. Indeed, infusions a new therapy healing, likely because autologous infusion at site injury might enhance responses, especially those driven by macrophages. this review, we summarize current knowledge on functions macrophages repairs their roles components promising regeneration.

Язык: Английский

Процитировано

45

DUX4 Role in Normal Physiology and in FSHD Muscular Dystrophy DOI Creative Commons
Emanuele Mocciaro, Valeria Runfola,

Paola Ghezzi

и другие.

Cells, Год журнала: 2021, Номер 10(12), С. 3322 - 3322

Опубликована: Ноя. 26, 2021

In the last decade, sequence-specific transcription factor double homeobox 4 (DUX4) has gone from being an obscure entity to a key in important physiological and pathological processes. We now know that expression of DUX4 is highly regulated restricted early steps embryonic development, where involved transcriptional activation zygotic genome. While epigenetically silenced most somatic tissues healthy humans, its aberrant reactivation associated with several diseases, including cancer, viral infection facioscapulohumeral muscular dystrophy (FSHD). also translocated, giving rise chimeric oncogenic proteins at basis sarcoma leukemia forms. Hence, understanding how performs activity could provide relevant information, not only further our knowledge human development regulation, but develop therapeutic approaches for diseases DUX4. Here, we summarize current on cellular molecular processes by special emphasis FSHD dystrophy.

Язык: Английский

Процитировано

45

Satellite cell contribution to disease pathology in Duchenne muscular dystrophy DOI Creative Commons
Kasun Kodippili, Michael A. Rudnicki

Frontiers in Physiology, Год журнала: 2023, Номер 14

Опубликована: Май 30, 2023

Progressive muscle weakness and degeneration characterize Duchenne muscular dystrophy (DMD), a lethal, x-linked neuromuscular disorder that affects 1 in 5,000 boys. Loss of dystrophin protein leads to recurrent degeneration, progressive fibrosis, chronic inflammation, dysfunction skeletal resident stem cells, called satellite cells. Unfortunately, there is currently no cure for DMD. In this mini review, we discuss how cells dystrophic are functionally impaired, contributes the DMD pathology, tremendous potential restoring endogenous cell function as viable treatment strategy treat debilitating fatal disease.

Язык: Английский

Процитировано

18

Evaluation of fermented soybean meal and edible insect hydrolysates as potential serum replacement in pig muscle stem cell culture DOI
Cho Hyun Kim, Hyun Jung Lee, Doo Yeon Jung

и другие.

Food Bioscience, Год журнала: 2023, Номер 54, С. 102923 - 102923

Опубликована: Июль 6, 2023

Язык: Английский

Процитировано

18