Autophagy in muscle regeneration: potential therapies for myopathies

Journal of Cachexia Sarcopenia and Muscle, Год журнала: 2022, Номер 13(3), С. 1673 - 1685

Опубликована: Апрель 17, 2022

Язык: Английский

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Elevated CD47 is a hallmark of dysfunctional aged muscle stem cells that can be targeted to augment regeneration

Cell stem cell, Год журнала: 2022, Номер 29(12), С. 1653 - 1668.e8

Опубликована: Ноя. 16, 2022

Язык: Английский

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Advances and Challenges in Cell Biology for Cultured Meat

Annual Review of Animal Biosciences, Год журнала: 2023, Номер 12(1), С. 345 - 368

Опубликована: Ноя. 14, 2023

Cultured meat is an emerging biotechnology that aims to produce from animal cell culture, rather than the raising and slaughtering of livestock, on environmental welfare grounds. The detailed understanding accurate manipulation biology are critical design cultured bioprocesses. Recent years have seen significant interest in this field, with numerous scientific commercial breakthroughs. Nevertheless, these technologies remain at a nascent stage, myriad challenges remain, spanning entire bioprocess. From biological perspective, include identification suitable starting types, tuning proliferation differentiation conditions, optimization cell–biomaterial interactions create nutritious, enticing foods. Here, we discuss key advances outstanding meat, particular focus biology, argue solving remaining bottlenecks cost-effective, scalable fashion will require coordinated, concerted efforts. Success also solutions nonscientific challenges, including regulatory approval, consumer acceptance, market feasibility. However, if can be overcome, revolutionize our approach food.

Язык: Английский

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The asymmetrical ESR1 signaling in muscle progenitor cells determines the progression of adolescent idiopathic scoliosis

Cell Discovery, Год журнала: 2023, Номер 9(1)

Опубликована: Апрель 25, 2023

Adolescent Idiopathic Scoliosis (AIS) is a common pediatric skeletal disease highly occurred in females. The pathogenesis of AIS has not been fully elucidated. Here, we reveal that ESR1 (Estrogen Receptor 1) expression declines muscle stem/progenitor cells at the concave side patients. Furthermore, required for cell differentiation and disrupted signaling leads to defects. imbalance para-spinal muscles induces scoliosis mice, while reactivation by an FDA approved drug Raloxifene alleviates curve progression. This work reveals asymmetric inactivation one causes AIS. Reactivation could be new strategy treat

Язык: Английский

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Perfusable adipose decellularized extracellular matrix biological scaffold co-recellularized with adipose-derived stem cells and L6 promotes functional skeletal muscle regeneration following volumetric muscle loss

Biomaterials, Год журнала: 2024, Номер 307, С. 122529 - 122529

Опубликована: Март 11, 2024

Язык: Английский

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IL-1β and TNF-α Modulation of Proliferated and Committed Myoblasts: IL-6 and COX-2-Derived Prostaglandins as Key Actors in the Mechanisms Involved

Cells, Год журнала: 2020, Номер 9(9), С. 2005 - 2005

Опубликована: Сен. 1, 2020

In this study, we investigated the effects and mechanisms of pro-inflammatory cytokines IL-1β TNF-α on proliferation commitment phases myoblast differentiation. C2C12 mouse cells were cultured to reach a proliferated or committed status incubated with these for evaluation cell proliferation, cyclooxygenase 2 (COX-2) expression, release prostaglandins (PGs) myokines, activation myogenic regulatory factors (MRFs). We found that inhibition IL-6 receptor reduced IL-1β- TNF-α-induced effect also involved COX-2-derived PGs. Both modulated myokines myostatin, irisin, osteonectin, IL-15. activity Pax7 in MyoD myogenin at both proliferative stages. Otherwise, increased only cells. Our data reveal key role PGs support link between MRFs. concluded induce similar initial stages muscle regeneration but critical differences their progression process, bringing new insights into inflammatory signalling skeletal regeneration.

Язык: Английский

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DUX4 Role in Normal Physiology and in FSHD Muscular Dystrophy

Cells, Год журнала: 2021, Номер 10(12), С. 3322 - 3322

Опубликована: Ноя. 26, 2021

In the last decade, sequence-specific transcription factor double homeobox 4 (DUX4) has gone from being an obscure entity to a key in important physiological and pathological processes. We now know that expression of DUX4 is highly regulated restricted early steps embryonic development, where involved transcriptional activation zygotic genome. While epigenetically silenced most somatic tissues healthy humans, its aberrant reactivation associated with several diseases, including cancer, viral infection facioscapulohumeral muscular dystrophy (FSHD). also translocated, giving rise chimeric oncogenic proteins at basis sarcoma leukemia forms. Hence, understanding how performs activity could provide relevant information, not only further our knowledge human development regulation, but develop therapeutic approaches for diseases DUX4. Here, we summarize current on cellular molecular processes by special emphasis FSHD dystrophy.

Язык: Английский

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Stem Cell and Macrophage Roles in Skeletal Muscle Regenerative Medicine

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(19), С. 10867 - 10867

Опубликована: Окт. 8, 2021

In severe muscle injury, skeletal tissue structure and functionality can be repaired through the involvement of several cell types, such as stem cells, innate immune responses. However, exact mechanisms behind regeneration, homeostasis, plasticity are still under investigation, discovery pathways types involved in repair open way for novel therapeutic approaches, cell-based therapies involving cells peripheral blood mononucleate cells. Indeed, infusions a new therapy healing, likely because autologous infusion at site injury might enhance responses, especially those driven by macrophages. this review, we summarize current knowledge on functions macrophages repairs their roles components promising regeneration.

Язык: Английский

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Machine learning inference of continuous single-cell state transitions during myoblast differentiation and fusion

Molecular Systems Biology, Год журнала: 2024, Номер unknown

Опубликована: Янв. 18, 2024

Abstract Cells modify their internal organization during continuous state transitions, supporting functions from cell division to differentiation. However, tools measure dynamic physiological states of individual transitioning cells are lacking. We combined live-cell imaging and machine learning monitor ERK1/2-inhibited primary murine skeletal muscle precursor cells, that transition rapidly robustly proliferating myoblasts post-mitotic myocytes then fuse, forming multinucleated myotubes. Our models, trained using motility or actin intensity features single-cell tracking data, effectively tracked real-time differentiation, revealing differentiation occurs 7.5–14.5 h post induction, followed by fusion ~3 later. Co-inhibition ERK1/2 p38 led without fusion. model inferred co-inhibition leads terminal indicating is specifically required for also predicted changes in dynamics. Mass spectrometry supported these silico predictions suggested novel maturation regulators downstream Collectively, this approach can be adapted various biological processes uncover links between functional outcomes.

Язык: Английский

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Bovine enhancer-regulated circSGCB acts as a ceRNA to regulate skeletal muscle development via enhancing KLF3 expression

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 261, С. 129779 - 129779

Опубликована: Янв. 28, 2024

Язык: Английский

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