Satellite cell contribution to disease pathology in Duchenne muscular dystrophy DOI Creative Commons
Kasun Kodippili, Michael A. Rudnicki

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: May 30, 2023

Progressive muscle weakness and degeneration characterize Duchenne muscular dystrophy (DMD), a lethal, x-linked neuromuscular disorder that affects 1 in 5,000 boys. Loss of dystrophin protein leads to recurrent degeneration, progressive fibrosis, chronic inflammation, dysfunction skeletal resident stem cells, called satellite cells. Unfortunately, there is currently no cure for DMD. In this mini review, we discuss how cells dystrophic are functionally impaired, contributes the DMD pathology, tremendous potential restoring endogenous cell function as viable treatment strategy treat debilitating fatal disease.

Language: Английский

Autophagy in muscle regeneration: potential therapies for myopathies DOI
Wei Chen, Yushi Chen, Yuxi Liu

et al.

Journal of Cachexia Sarcopenia and Muscle, Journal Year: 2022, Volume and Issue: 13(3), P. 1673 - 1685

Published: April 17, 2022

Language: Английский

Citations

50
Elevated CD47 is a hallmark of dysfunctional aged muscle stem cells that can be targeted to augment regeneration DOI Creative Commons
Ermelinda Porpiglia,

Thach Mai,

Peggy E. Kraft

et al.

Cell stem cell, Journal Year: 2022, Volume and Issue: 29(12), P. 1653 - 1668.e8

Published: Nov. 16, 2022

Language: Английский

Citations

46
Advances and Challenges in Cell Biology for Cultured Meat DOI Creative Commons
Beatriz T. Martins,

Arthur Bister,

Richard G. J. Dohmen

et al.

Annual Review of Animal Biosciences, Journal Year: 2023, Volume and Issue: 12(1), P. 345 - 368

Published: Nov. 14, 2023

Cultured meat is an emerging biotechnology that aims to produce from animal cell culture, rather than the raising and slaughtering of livestock, on environmental welfare grounds. The detailed understanding accurate manipulation biology are critical design cultured bioprocesses. Recent years have seen significant interest in this field, with numerous scientific commercial breakthroughs. Nevertheless, these technologies remain at a nascent stage, myriad challenges remain, spanning entire bioprocess. From biological perspective, include identification suitable starting types, tuning proliferation differentiation conditions, optimization cell–biomaterial interactions create nutritious, enticing foods. Here, we discuss key advances outstanding meat, particular focus biology, argue solving remaining bottlenecks cost-effective, scalable fashion will require coordinated, concerted efforts. Success also solutions nonscientific challenges, including regulatory approval, consumer acceptance, market feasibility. However, if can be overcome, revolutionize our approach food.

Language: Английский

Citations

31
The asymmetrical ESR1 signaling in muscle progenitor cells determines the progression of adolescent idiopathic scoliosis DOI Creative Commons
Xiexiang Shao, Xin Fu,

Jingfan Yang

et al.

Cell Discovery, Journal Year: 2023, Volume and Issue: 9(1)

Published: April 25, 2023

Adolescent Idiopathic Scoliosis (AIS) is a common pediatric skeletal disease highly occurred in females. The pathogenesis of AIS has not been fully elucidated. Here, we reveal that ESR1 (Estrogen Receptor 1) expression declines muscle stem/progenitor cells at the concave side patients. Furthermore, required for cell differentiation and disrupted signaling leads to defects. imbalance para-spinal muscles induces scoliosis mice, while reactivation by an FDA approved drug Raloxifene alleviates curve progression. This work reveals asymmetric inactivation one causes AIS. Reactivation could be new strategy treat

Language: Английский

Citations

25
Perfusable adipose decellularized extracellular matrix biological scaffold co-recellularized with adipose-derived stem cells and L6 promotes functional skeletal muscle regeneration following volumetric muscle loss DOI
Wei Liang, Meng Han, Li Guan

et al.

Biomaterials, Journal Year: 2024, Volume and Issue: 307, P. 122529 - 122529

Published: March 11, 2024

Language: Английский

Citations

11
Machine learning inference of continuous single-cell state transitions during myoblast differentiation and fusion DOI Creative Commons

Amit Shakarchy,

Giulia Zarfati, Adi Hazak

et al.

Molecular Systems Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 18, 2024

Abstract Cells modify their internal organization during continuous state transitions, supporting functions from cell division to differentiation. However, tools measure dynamic physiological states of individual transitioning cells are lacking. We combined live-cell imaging and machine learning monitor ERK1/2-inhibited primary murine skeletal muscle precursor cells, that transition rapidly robustly proliferating myoblasts post-mitotic myocytes then fuse, forming multinucleated myotubes. Our models, trained using motility or actin intensity features single-cell tracking data, effectively tracked real-time differentiation, revealing differentiation occurs 7.5–14.5 h post induction, followed by fusion ~3 later. Co-inhibition ERK1/2 p38 led without fusion. model inferred co-inhibition leads terminal indicating is specifically required for also predicted changes in dynamics. Mass spectrometry supported these silico predictions suggested novel maturation regulators downstream Collectively, this approach can be adapted various biological processes uncover links between functional outcomes.

Language: Английский

Citations

9
IL-1β and TNF-α Modulation of Proliferated and Committed Myoblasts: IL-6 and COX-2-Derived Prostaglandins as Key Actors in the Mechanisms Involved DOI Creative Commons
Ángela M. Álvarez, Carlos DeOcesano-Pereira, Catarina Teixeira

et al.

Cells, Journal Year: 2020, Volume and Issue: 9(9), P. 2005 - 2005

Published: Sept. 1, 2020

In this study, we investigated the effects and mechanisms of pro-inflammatory cytokines IL-1β TNF-α on proliferation commitment phases myoblast differentiation. C2C12 mouse cells were cultured to reach a proliferated or committed status incubated with these for evaluation cell proliferation, cyclooxygenase 2 (COX-2) expression, release prostaglandins (PGs) myokines, activation myogenic regulatory factors (MRFs). We found that inhibition IL-6 receptor reduced IL-1β- TNF-α-induced effect also involved COX-2-derived PGs. Both modulated myokines myostatin, irisin, osteonectin, IL-15. activity Pax7 in MyoD myogenin at both proliferative stages. Otherwise, increased only cells. Our data reveal key role PGs support link between MRFs. concluded induce similar initial stages muscle regeneration but critical differences their progression process, bringing new insights into inflammatory signalling skeletal regeneration.

Language: Английский

Citations

56
Stem Cell and Macrophage Roles in Skeletal Muscle Regenerative Medicine DOI Open Access
Pasqualina Scala,

Laura Rehak,

Valentina Giudice

et al.

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(19), P. 10867 - 10867

Published: Oct. 8, 2021

In severe muscle injury, skeletal tissue structure and functionality can be repaired through the involvement of several cell types, such as stem cells, innate immune responses. However, exact mechanisms behind regeneration, homeostasis, plasticity are still under investigation, discovery pathways types involved in repair open way for novel therapeutic approaches, cell-based therapies involving cells peripheral blood mononucleate cells. Indeed, infusions a new therapy healing, likely because autologous infusion at site injury might enhance responses, especially those driven by macrophages. this review, we summarize current knowledge on functions macrophages repairs their roles components promising regeneration.

Language: Английский

Citations

43
DUX4 Role in Normal Physiology and in FSHD Muscular Dystrophy DOI Creative Commons
Emanuele Mocciaro, Valeria Runfola,

Paola Ghezzi

et al.

Cells, Journal Year: 2021, Volume and Issue: 10(12), P. 3322 - 3322

Published: Nov. 26, 2021

In the last decade, sequence-specific transcription factor double homeobox 4 (DUX4) has gone from being an obscure entity to a key in important physiological and pathological processes. We now know that expression of DUX4 is highly regulated restricted early steps embryonic development, where involved transcriptional activation zygotic genome. While epigenetically silenced most somatic tissues healthy humans, its aberrant reactivation associated with several diseases, including cancer, viral infection facioscapulohumeral muscular dystrophy (FSHD). also translocated, giving rise chimeric oncogenic proteins at basis sarcoma leukemia forms. Hence, understanding how performs activity could provide relevant information, not only further our knowledge human development regulation, but develop therapeutic approaches for diseases DUX4. Here, we summarize current on cellular molecular processes by special emphasis FSHD dystrophy.

Language: Английский

Citations

43
Evaluation of fermented soybean meal and edible insect hydrolysates as potential serum replacement in pig muscle stem cell culture DOI
Cho Hyun Kim, Hyun Jung Lee, Doo Yeon Jung

et al.

Food Bioscience, Journal Year: 2023, Volume and Issue: 54, P. 102923 - 102923

Published: July 6, 2023

Language: Английский

Citations

17