Functions of Circular RNA in Human Diseases and Illnesses

Non-Coding RNA, Год журнала: 2023, Номер 9(4), С. 38 - 38

Опубликована: Июль 4, 2023

Circular RNAs (circRNAs) represent single-stranded RNA species that contain covalently closed 3′ and 5′ ends provide them more stability than linear RNA, which has free ends. Emerging evidence indicates circRNAs perform essential functions in many DNA viruses, including coronaviruses, Epstein–Barr cytomegalovirus, Kaposi sarcoma viruses. Recent studies have confirmed are present play various important such as evading host immune response, disease pathogenesis, protein translation, miRNA sponges, regulating cell proliferation, virus replication. Studies can be biological signatures or pathological markers for autoimmune diseases, neurological cancers. However, our understanding of viruses is still limited, functional evaluation viral to completely understand their functions. In the review, we describe metabolism cellular roles circRNA, its diseases circRNA found interact with proteins, DNA, thus modulate processes, transcription, splicing, other interfere signaling pathways take part vital biological, physiological, cellular, pathophysiological processes. We also summarize recent demonstrating circRNA’s this growing field research.

Язык: Английский

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Emerging role and clinical applications of circular RNAs in human diseases

Functional & Integrative Genomics, Год журнала: 2025, Номер 25(1)

Опубликована: Март 28, 2025

Язык: Английский

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Identification of circulating microRNA profiles associated with pulmonary function and radiologic features in survivors of SARS-CoV-2-induced ARDS

Emerging Microbes & Infections, Год журнала: 2022, Номер 11(1), С. 1537 - 1549

Опубликована: Май 23, 2022

There is a limited understanding of the pathophysiology postacute pulmonary sequelae in severe COVID-19. The aim current study was to define circulating microRNA (miRNA) profiles associated with function and radiologic features survivors SARS-CoV-2-induced ARDS. included patients who developed ARDS secondary SARS-CoV-2 infection (n = 167) group infected did not develop 33). Patients were evaluated 3 months after hospital discharge. follow-up complete evaluation chest computed tomography. Plasma miRNA profiling performed using RT-qPCR. Random forest used construct signatures lung diffusing capacity for carbon monoxide (DLCO) total severity score (TSS). Kyoto Encyclopedia Genes Genomes (KEGG) Gene Ontology (GO) enrichment analyses conducted. DLCO < 80% predicted observed 81.8% patients. TSS showed median [P25;P75] 5 [2;8]. model comprised miR-17-5p, miR-27a-3p, miR-126-3p, miR-146a-5p miR-495-3p. Concerning features, signature composed by miR-9-5p, miR-21-5p, miR-24-3p miR-221-3p correlated values. These associations non-ARDS group. KEGG pathway GO provided evidence molecular mechanisms related only profibrotic or anti-inflammatory states but also cell death, immune response, hypoxia, vascularization, coagulation viral infection. In conclusion, radiological from are specific profiles. findings provide novel insights into possible pathways underlying pathogenesis sequelae.Trial registration: ClinicalTrials.gov identifier: NCT04457505..Trial ISRCTN.org ISRCTN16865246..

Язык: Английский

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The regulation of lncRNAs and miRNAs in SARS-CoV-2 infection

Frontiers in Cell and Developmental Biology, Год журнала: 2023, Номер 11

Опубликована: Июль 27, 2023

The coronavirus disease 2019 (COVID-19) was a global endemic that continues to cause large number of severe illnesses and fatalities. There is increasing evidence non-coding RNAs (ncRNAs) are crucial regulators viral infection antiviral immune response the role in SARS-CoV-2 has now become focus scholarly inquiry. After infection, some ncRNAs’ expression levels regulated indirectly control genes gene replication. However, other ncRNAs hijacked by order help virus evade system suppressing type I interferon (IFN-1) controlling cytokine levels. In this review, we summarize recent findings long (lncRNAs) microRNAs (miRNAs) among response, discuss potential mechanisms actions, prospects for detection, treatment, prevention future directions research.

Язык: Английский

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Circular RNAs in the pathogenesis of SARS-CoV-2: potential diagnostic biomarkers and therapeutic targets

Functional & Integrative Genomics, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 2, 2025

Язык: Английский

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CircSARS-CV2-N1368 from SARS-CoV-2 impairs endothelial cell function through the upregulation of ATF7 to activate TLR4/NF-κB/ROS signaling

Acta Pharmacologica Sinica, Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

Abstract SARS-CoV-2 can encode circular RNAs (circRNAs); however, the potential effects of exogenous circRNAs on cardiovascular sequelae remain unknown. Three derived from nucleocapsid (N) gene SARS-CoV-2, namely, circSARS-CV2-Ns, were identified for functional studies. In particular, circSARS-CV2-N1368 was shown to enhance platelet adhesiveness endothelial cells (ECs) and inhibit EC-dependent vascular relaxation. Moreover, expression suppressed EC proliferation migration decreased angiogenesis cardiac organoid beating. Mechanistically, we elucidated that sponged microRNA miR-103a-3p, which could reverse circSARS-CV2-N1368-induced damage. Additionally, activating transcription factor 7 (ATF7) as a target Toll-like receptor 4 (TLR4) verified downstream ATF7 mediates circARS-CV2-N1368-induced activation nuclear kappa B (NF-κB) signaling ROS production in ECs. Importantly, reactive oxygen species (ROS) scavenger NAC mitigated circSARS-CV2-N1368-promoted impairment. Our findings reveal TLR4/NF-κB/ROS signal pathway is critical mediating oxidative damage ECs, providing insights into impairment caused by circSARS-CV2-Ns.

Язык: Английский

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Hsa_circ_0007321 regulates Zika virus replication through miR-492/NFKBID/NF-κB signaling pathway

Journal of Virology, Год журнала: 2023, Номер 97(12)

Опубликована: Дек. 5, 2023

Over the past decade, increasing evidence has shown that circular RNAs (circRNAs) play important regulatory roles in viral infection and host antiviral responses. However, reports on role of circRNAs Zika virus (ZIKV) are limited. In this study, we identified 45 differentially expressed ZIKV-infected A549 cells by RNA sequencing. We clarified a downregulated circRNA, hsa_circ_0007321, regulates ZIKV replication through targeting miR-492 downstream gene NFKBID. NFKBID is negative regulator nuclear factor-κB (NF-κB), found inhibition NF-κB pathway promotes replication. Therefore, finding hsa_circ_0007321 exerts its miR-492/NFKBID/NF-κB signaling implications for development strategies to suppress possibly other infections.

Язык: Английский

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circSMARCA5 Is an Upstream Regulator of the Expression of miR-126-3p, miR-515-5p, and Their mRNA Targets, Insulin-like Growth Factor Binding Protein 2 (IGFBP2) and NRAS Proto-Oncogene, GTPase (NRAS) in Glioblastoma

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(22), С. 13676 - 13676

Опубликована: Ноя. 8, 2022

The involvement of non-coding RNAs (ncRNAs) in glioblastoma multiforme (GBM) pathogenesis and progression has been ascertained but their cross-talk within GBM cells remains elusive. We previously demonstrated the role circSMARCA5 as a tumor suppressor (TS) GBM. In this paper, we explore control microRNA (miRNA) expression By using TaqMan® low-density arrays, 748 miRNAs was assayed U87MG overexpressing circSMARCA5. Differentially expressed (DE) were validated through single assays in: (i) circSMARCA5; (ii) four additional cell lines (A172; CAS-1; SNB-19; U251MG); (iii) thirty-eight biopsies; (iv) twenty biopsies unaffected brain parenchyma (UC). Validated targets DE selected from databases TarBase miRTarbase, literature; inferred TCGA dataset. Expression circSMARCA5, lines, real-time PCR. TS 126-3p 515-5p upregulated following overexpression positively correlated with that (r-values = 0.49 0.50, p-values 9 × 10−5 7 10−5, respectively) biopsies. Among targets, IGFBP2 (target miR-126-3p) NRAS miR-515-5p) mRNAs (r-value 0.46, p-value 0.00027), while negatively −0.58 −0.30, 0 0.019, respectively), miR-126-3p −0.36, 0.0066), miR-515-5p −0.34, 0.010), respectively. Our data identified new subnetwork controlled by which regulates downstream 515-5p, mRNA NRAS.

Язык: Английский

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circRNA-SMO upregulates CEP85 to promote proliferation and migration of glioblastoma via sponging miR-326.

PubMed, Год журнала: 2023, Номер 38(11), С. 1307 - 1319

Опубликована: Ноя. 1, 2023

Circular RNAs (circRNAs) play an important role in cancer development by sponging microRNAs (miRNAs) to regulate the signaling axis. However, more comprehensive mechanisms of circRNAs glioblastoma need be elucidated. RT-qPCR was used detect expression levels circRNA-SMO and miR-326. Dual-luciferase reporter assays were conducted verify interaction among circRNA-SMO, miR-326, CEP85. Flow cytometric analysis performed apoptosis. Western blotting determine protein different molecules. Animal xenograft experiments evaluate vivo. CircRNA-SMO upregulated tissues cells. downregulation inhibited viability colony-forming ability In addition, miR-326 downregulated cells, which verified sponge interact with Moreover, inhibition induced elevation apoptosis, accompanied a decrease knockdown-mediated tumor prevented inhibitor. Furthermore, growth vivo, increase decline CEP85 tissues. Conclusions. sponges promote proliferation migration upregulating expression. This study clarified glioblastoma, providing novel insights for its treatment.

Язык: Английский

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New insights on circular RNAs and their potential applications as biomarkers, therapeutic agents, and preventive vaccines in viral infections: with a glance at SARS-CoV-2

Molecular Therapy — Nucleic Acids, Год журнала: 2022, Номер 29, С. 705 - 717

Опубликована: Авг. 14, 2022

Язык: Английский

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