Enhanced Tumor Immunotherapy by Triple Amplification Effects of Nanomedicine on the STING Signaling Pathway in Dendritic Cells DOI
Xiangyu Wang, Yi Yan, Xia Guo

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Окт. 23, 2024

Abstract Insufficient activation of stimulator interferon genes (STING) signaling pathway in tumor‐associated dendritic cells limits the efficiency tumor immunotherapy. Herein, “three‐in‐one” IAHA‐LaP/siPTPN6 NPs containing lanthanum ions (La 3+ ), cGAMP, and PTPN6 siRNA are developed for triple amplification STING pathway. In vitro results demonstrate that La significantly promotes cGAMP‐mediated by enhancing phosphorylation STING, TBK1, IRF3, NF‐ κ B p65. Moreover, further enhance p65 augment K63‐linked ubiquitination protein via siPTPN6‐mediated downregulation SHP‐1 protein. Furthermore, improve secretion IFN β (2.4‐fold), IL‐6 (1.5‐fold), TNF‐ α (1.4‐fold), thereby promoting DCs maturation compared to mixture cGAMP. vivo show remarkably inhibit primary growth increasing percentage mature tumor‐draining lymph nodes, polarizing M2/M1 phenotype TME, infiltration CD8 + T into tumors. these dramatically prevent distal inducing systemic anti‐tumor immunity generating a long‐term memory protection against recurrence mice bearing bilateral B16F10. These may offer promising platform robust immune responses.

Язык: Английский

Manganese improves anti-PD-L1 immunotherapy via eliciting type I interferon signaling in melanoma DOI
Xiao-Xin Zhang,

Jianhua Deng,

Renjie Wu

и другие.

Investigational New Drugs, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 27, 2024

Язык: Английский

Процитировано

1
Lymph node-targeted STING agonist nanovaccine against chronic HBV infection DOI Creative Commons
Yifei Hu,

Ailu Yang,

Hui Li

и другие.

Cellular and Molecular Life Sciences, Год журнала: 2024, Номер 81(1)

Опубликована: Авг. 28, 2024

Chronic hepatitis B virus (HBV) infection is a global health problem that substantially increases the risk of developing liver disease. The development novel strategy to induce anti-HB seroconversion and achieve long-lasting immune response against chronic HBV remains challenging. Here, we found affected signaling pathway involved in STING-mediated induction host responses dendritic cells (DCs) then generated lymph node-targeted nanovaccine co-delivered surface antigen (HBsAg) cyclic diguanylate monophosphate (c-di-GMP) (named PP-SG nanovaccine). feasibility efficiency for CHB treatment were evaluated HBV-carrier mice. Serum samples analyzed HBsAg, anti-HBs, DNA, alanine aminotransferase levels, DNA RNA HBcAg, accompanied by an analysis HBV-specific cellular humoral during treatment. increased phagocytosis DC maturation, efficiently safely eliminated HBV, achieved reinjection, disrupted infection-induced tolerance, as characterized generation multifunctionality CD8

Язык: Английский

Процитировано

0
CRISPR-Mediated Construction of Gene-Knockout Mice for Investigating Antiviral Innate Immunity DOI

Yangkun Shen,

Xiangqian Zhao,

Chunfu Zheng

и другие.

Methods in molecular biology, Год журнала: 2024, Номер unknown, С. 61 - 74

Опубликована: Авг. 27, 2024

Язык: Английский

Процитировано

0
Enhanced Tumor Immunotherapy by Triple Amplification Effects of Nanomedicine on the STING Signaling Pathway in Dendritic Cells DOI
Xiangyu Wang, Yi Yan, Xia Guo

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Окт. 23, 2024

Abstract Insufficient activation of stimulator interferon genes (STING) signaling pathway in tumor‐associated dendritic cells limits the efficiency tumor immunotherapy. Herein, “three‐in‐one” IAHA‐LaP/siPTPN6 NPs containing lanthanum ions (La 3+ ), cGAMP, and PTPN6 siRNA are developed for triple amplification STING pathway. In vitro results demonstrate that La significantly promotes cGAMP‐mediated by enhancing phosphorylation STING, TBK1, IRF3, NF‐ κ B p65. Moreover, further enhance p65 augment K63‐linked ubiquitination protein via siPTPN6‐mediated downregulation SHP‐1 protein. Furthermore, improve secretion IFN β (2.4‐fold), IL‐6 (1.5‐fold), TNF‐ α (1.4‐fold), thereby promoting DCs maturation compared to mixture cGAMP. vivo show remarkably inhibit primary growth increasing percentage mature tumor‐draining lymph nodes, polarizing M2/M1 phenotype TME, infiltration CD8 + T into tumors. these dramatically prevent distal inducing systemic anti‐tumor immunity generating a long‐term memory protection against recurrence mice bearing bilateral B16F10. These may offer promising platform robust immune responses.

Язык: Английский

Процитировано

0