Integrating GWAS, RNA‐Seq and functional analysis revealed that BnaA02.SE mediates silique elongation by affecting cell proliferation and expansion in Brassica napus

Plant Biotechnology Journal, Год журнала: 2024, Номер 22(10), С. 2907 - 2920

Опубликована: Июнь 20, 2024

Summary Rapeseed ( Brassica napus ) silique is the major carbohydrate source for seed development, and final length has attracted great attention from breeders. However, no studies had focused on dynamic character of elongation (SEL). Here, SEL investigation in a natural population including 588 lines over two years indicate that during 0–20 days after flowering was most essential stage associated with number per (SPS) thousand weight (TSW). Then, nine loci were identified to be based GWAS analysis, among which five SNPs (over 50%) distributed A02 chromosome within 6.08 6.48 Mb. Subsequently, we screened 5078 differentially expressed genes between extreme materials. An unknown protein, BnaA02.SE, combining RNA‐Seq analysis. Subcellular localization expression profiles analysis demonstrated BnaA02.SE chloroplast‐ nucleus‐localized protein mainly pericarps leaves. Furthermore, transgenic verification cytological observation reveal overexpressed can promote by regulating JA IAA contents, affecting cell proliferation expansion, respectively, finally enhance yield influencing SPS TSW. Haplotype homologs may also involved regulation. Our findings provided comprehensive insights into newly trait, cloned first gene controlling B. . The its offer valuable target improving yield.

Язык: Английский

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Dissection of the genetic basis and molecular mechanism of ovule number per ovary in oilseed rape (Brassica napus L.)

Frontiers in Plant Science, Год журнала: 2025, Номер 15

Опубликована: Янв. 28, 2025

Ovule number per ovary (ONPO) determines the maximum potential of seed fruit that is a direct component yield in crops. This study aimed to dissect genetic basis and molecular mechanism ONPO using newly developed doubled haploid (DH) population oilseed rape. In all four investigated environments, 201 DH lines exhibited normal distribution with wide variation from 22.6 41.8, suggesting quantitative inheritance appropriate for mapping QTL. A skeleton map 2111 markers within 19 linkage groups was developed, total 1715.71 cM length an average 0.82 between markers. Linkage identified ten QTLs were distributed on eight chromosomes explained 7.0-15.9% phenotypic variance. Among these, identical reported two repeatedly detected relatively large effects, highlighting their marker-assisted selection. Phytohormone quantification ovaries (at ovule initiation stage) pools high low showed significant differences levels nine sub-types phytohormones, important roles regulating number. Transcriptomic analysis 7689 differentially expressed genes (DEGs) pools, which nearly half enriched into functional categories ONPO, including protein, RNA, signalling, miscellaneous, development, hormone metabolism, tetrapyrrole synthesis. Integration QTL mapping, transcriptome sequencing BLAST 15 homologues 327 DEGs regions, considered as candidate genes. These findings propose further insights mechanisms will facilitate future gene cloning improvement enhancing

Язык: Английский

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Genomics, Pan-Genomics, and Super Pan-Genomics of Major Oilseed Crops

Опубликована: Янв. 1, 2025

Язык: Английский

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Fine mapping and candidate gene analysis of the major QTL qSW-A03 for seed weight in Brassica napus

Theoretical and Applied Genetics, Год журнала: 2025, Номер 138(4)

Опубликована: Март 17, 2025

Язык: Английский

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Genome-wide association study reveals genetic loci for seed density per silique in rapeseed (Brassica napus L.)

Theoretical and Applied Genetics, Год журнала: 2025, Номер 138(4)

Опубликована: Март 20, 2025

Язык: Английский

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Zinc deficiency responses: bridging the gap between Arabidopsis and dicotyledonous crops

Journal of Experimental Botany, Год журнала: 2021, Номер 73(6), С. 1699 - 1716

Опубликована: Ноя. 5, 2021

Abstract Zinc (Zn) deficiency is a widespread phenomenon in agricultural soils worldwide and has major impact on crop yield quality, hence human nutrition health. Although dicotyledonous crops represent >30% of plant-based nutrition, relatively few efforts have been dedicated to the investigation Zn response mechanisms dicotyledonous, contrast monocotyledonous crops, such as rice or barley. Here, we describe requirement several economically important Phaseolus vulgaris, Glycine max, Brassica oleracea, Solanum lycopersicum. We briefly review our current knowledge Arabidopsis outline how this translated crops. highlight commonalities differences between species (and with species) regarding function regulation transporters chelators, well Zn-sensing role hormones response. Moreover, show homeostatic network intimately interacts other nutrients, iron phosphate. Finally, variation tolerance use efficiency among cultivars can be leveraged for design biofortification strategies.

Язык: Английский

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3D genome structural variations play important roles in regulating seed oil content of Brassica napus

Plant Communications, Год журнала: 2023, Номер 5(1), С. 100666 - 100666

Опубликована: Июль 26, 2023

Dissecting the complex regulatory mechanism of seed oil content (SOC) is one main research goals in Brassica napus. Increasing evidence suggests that genome architecture linked to multiple biological functions. However, effect on SOC regulation remains unclear. Here, we used high-throughput chromatin conformation capture characterize differences three-dimensional (3D) landscape seeds from two B. napus lines, N53-2 (with high SOC) and Ken-C8 low SOC). Bioinformatics analysis demonstrated differentially accessible regions expressed genes between were preferentially enriched with quantitative trait loci (QTLs)/associated genomic (AGRs) for SOC. A multi-omics expression SOC-related was tightly correlated structural variations QTLs/AGRs The candidate gene BnaA09g48250D, which showed variation a QTL/AGR chrA09, identified by fine-mapping KN double-haploid population derived hybridization Ken-C8. Overexpression knockout BnaA09g48250D led significant increases decreases SOC, respectively, transgenic lines. Taken together, our results reveal 3D roles regulation, enriching understanding molecular mechanisms perspective spatial structure.

Язык: Английский

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An Application of Rock Phosphate Increased Soil Cadmium Contamination and Hampered the Morphophysiological Growth of Brassica campestris L

Journal of soil science and plant nutrition, Год журнала: 2023, Номер 23(3), С. 4583 - 4595

Опубликована: Июль 24, 2023

Язык: Английский

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Linkage and association mapping of ovule number per ovary (ON) in oilseed rape (Brassica napus L.)

Molecular Breeding, Год журнала: 2023, Номер 43(2)

Опубликована: Фев. 1, 2023

Язык: Английский

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QTL mapping for seed density per silique in Brassica napus

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Янв. 14, 2023

Abstract Seed density per silique (SDPS) and valid length (VSL) are two important yield-influencing traits in rapeseed. SDPS has a direct or indirect effect on rapeseed yield through its seed silique. In this study, quantitative trait locus (QTL) for was detected chromosome A09 using the QTL-seq approach confirmed via linkage analysis mapping population obtained from 4263 × 3001 cross. Furthermore, one major QTL ( qSD.A9-1 ) mapped to 401.8 kb genomic interval between SSR markers Nys9A190 Nys9A531 . same region, qSL.A9) linked VSL also detected. The phenotypic variation of qSL.A9 53.1% 47.6%, respectively. Results additive dominant effects demonstrated that expression genes controlling were derived different parent population. Subsequently, we identified 56 included 45 specific with exonic (splicing) variants. Further containing mutations may be related as well length. These could used further studies understand details these

Язык: Английский

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