Oil Crop Science, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 1, 2024
Язык: Английский
Journal of Integrative Plant Biology, Год журнала: 2024, Номер 66(3), С. 484 - 509
Опубликована: Март 1, 2024
ABSTRACT Brassica napus , commonly known as rapeseed or canola, is a major oil crop contributing over 13% to the stable supply of edible vegetable worldwide. Identification and understanding gene functions in B. genome crucial for genomic breeding. A group genes controlling agronomic traits have been successfully cloned through functional genomics studies . In this review, we present an overview progress made including availability germplasm resources, omics databases genes. Based on current progress, also highlight main challenges perspectives field. The advances contribute better genetic basis underlying complex will expedite breeding high quality, resistance yield varieties.
Язык: Английский
Процитировано
18
Science Advances, Год журнала: 2025, Номер 11(1)
Опубликована: Янв. 3, 2025
Yellow seed coat color (SCC) is a valuable trait in Brassica napus , which significantly correlated to high oil content (SOC) and low lignocellulose (SLC). However, no dominant yellow SCC genes were identified B. . In this study, N53-2 was verified, then 58,981 eQTLs 25 trans-eQTL hotspots double haploid population derived from black material Ken-C8. A rare allele DYSOC1 ( gene of improved 1 ) subsequently cloned hotspot that colocated with SCC, SOC, SLC QTL on ChrA09 through fine mapping multi-omics analysis. Transgenic experiments revealed the expression produced seeds increased SOC decreased SLC. Our result provides has excellent potential for rapeseed breeding.
Язык: Английский
Процитировано
1
Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 17, 2025
ABSTRACT Plant oils play a crucial role in human nutrition, industrial applications and biofuel production. While the enzymes involved fatty acid (FA) biosynthesis are well‐studied, regulatory networks governing these processes remain largely unexplored. This review explores intricate modulating seed oil biosynthesis, focusing on key pathways factors. Seed content is determined by efficiency of de novo FA synthesis as well influenced sugar transport, lipid metabolism, inhibitors fine‐tuning mechanisms. At center this network WRINKLED1 (WRI1), which plays conserved promoting across various plant species. WRI1 interacts with multiple proteins, its expression level regulated upstream regulators, including members LAFL network. Beyond network, we also discuss potential nuclear factor‐Y (NF‐Y) soybean an emphasis NF‐YA NF‐YB their associated proteins. NF‐Y represents promising avenue for future efforts aimed at enhancing accumulation improving stress tolerance soybean. Additionally, application omics‐based approaches great significance. Advances omics technologies have greatly facilitated identification gene resources, opening new opportunities genetic improvement. Importantly, several transcription factors participate responses, highlighting link between two processes. comprehensive elucidates complex mechanisms underlying regulation offering insights into biotechnological strategies production crops.
Язык: Английский
Процитировано
1
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Progress in Lipid Research, Год журнала: 2024, Номер unknown, С. 101306 - 101306
Опубликована: Ноя. 1, 2024
Язык: Английский
Процитировано
2
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июнь 28, 2024
Abstract Yellow pitaya ( Selenicereus megalanthus , 2n=4x=44) breeding remains severely hindered due to lacking a reference genome. Here we report yellow pitaya’s high-quality chromosome-level genome assembly and link the phenotypic trait with genomic data, based on Hi-C, ATAC, RNA-seq data of specific tissues. We declared as an autotetraploid 7.16 Gb size (harboring 27,246 high confidence genes) majorly evolved from diploid ancestors, which unknown. Beyond generating assembly, explored 3D chromatin organization revealed insights into genome, compartments A (648 519), B (728 1064), topologically associated domains-TADs (3376 2031), varying numbers structural variations (SVs) in polyploid species, respectively. Overall, TAD boundaries were enriched motifs AP2 WRKY18/60/75 MYB63/116 PHL2 GATA8 both species. By linking open structure function, identified major changes betalains biosynthesis pathway pitaya. Moreover, higher genetic expression SmeADH1 [Chr11, Compartment (135400000 - 135500000), genes inside region (135480000 135520000)], lower HuDOPA (87100000 87200000), (87160000-87200000)] acts key regulator red color pericarp pitaya, In addition, HuCYP76AD1 SmeCYP76AD1 potentially created difference oxidase process that led production betacyanin betaxanthin, Furthermore, our results not only type play potential role patterning but also further uncovered motif count TAD-boundaries may impact gene within regions Our valuable resource comparison euchromatin architecture species will aid advancement molecular efforts offer genomes, SVs, compartmentalization (A B), TADs, have strengthen idea TADs-based improvement achieve global food security.
Язык: Английский
Процитировано
1
Advanced Genetics, Год журнала: 2023, Номер 5(1)
Опубликована: Ноя. 27, 2023
Abstract Plant biology studies in the post‐genome era have been focused on annotating genome sequences’ functions. The established plant mutant collections greatly accelerated functional genomics research past few decades. However, most sequences' roles and underlying regulatory networks remain substantially unknown. Clustered, regularly interspaced short palindromic repeat (CRISPR)‐associated systems are robust, versatile tools for manipulating genomes with various targeted DNA perturbations, providing an excellent opportunity high‐throughput interrogation of elements’ roles. This study compares methods frequently used then discusses different multi‐targeted strategies to overcome gene redundancy using CRISPR‐Cas9 system. Next, this work summarizes recent reports CRISPR libraries knockout function discoveries plants. Finally, envisions future perspective optimizing leveraging library screening genomes' other uncharacterized sequences.
Язык: Английский
Процитировано
2
Oil Crop Science, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 1, 2024
Язык: Английский
Процитировано
0