Bioactive Metabolite from Endophytic Aspergillus versicolor SB5 with Anti-Acetylcholinesterase, Anti-Inflammatory and Antioxidant Activities: In Vitro and In Silico Studies

Microorganisms, Год журнала: 2023, Номер 11(4), С. 1062 - 1062

Опубликована: Апрель 19, 2023

Endophytic fungi are a highly unpredictable group of microorganisms that can create diverse range secondary metabolites with biological activity. These enhance the host's ability to tolerate stress caused by various factors, such as disease, insects, pathogens, and herbivores. The produced endophytic may have potential applications in agriculture, pharmacy, medicine. purpose this study was examine anti-acetylcholinesterase activity extracted from fungi. Aspergillus versicolor SB5 one many isolated Juncus rigidus identified genetically accession number ON872302. Our utilized fermentation microbial cultivation techniques obtain metabolites. During course our investigation, we compound called Physcion (C1) fungus SB5. We subsequently C1 possesses inhibitory against COX-2 LOX-1, IC50 values 43.10 17.54 µg/mL, respectively, making it an effective anti-inflammatory agent. Moreover, found also exhibited potent anticholinesterase (86.9 ± 1.21%). In addition these promising therapeutic properties, experiments demonstrated strong antioxidant capacity, evidenced its scavenge DPPH, ABTS, O2 radicals, NO inhibit lipid peroxidation. To further investigate molecular mechanisms underlying pharmacological employed SwissADME web tools predict compound's ADME-related physicochemical properties used Molecular Operating Environment PyMOL for docking studies.

Язык: Английский

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Environmental factors on secondary metabolism in medicinal plants: exploring accelerating factors

Medicinal Plant Biology, Год журнала: 2024, Номер 3(1), С. 0 - 0

Опубликована: Янв. 1, 2024

Medicinal plants are vital in synthesizing crucial substrates, fortifying stress resilience, and serving clinical industrial domains. The optimization of pharmacological potential necessitates a nuanced understanding the factors governing synthesis secondary metabolites sourced from plants. Cultivation success hinges upon many dictating production these compounds. Biotic factors, encompassing pathogens herbivores, alongside abiotic such as light exposure, altitude, temperature variations, irrigation patterns, soil fertility, drought susceptibility, salinity levels, collectively orchestrate medicinal plants' growth, development, metabolic pathways. This comprehensive review delves into intricate interplay influencing formation metabolites, exploring roles endophytes, pathogens, availability, fluctuations, stress, pollution impacts, plant growth regulators. Grasping dynamics is imperative for devising strategic interventions to enhance metabolite production, thereby ensuring sustainable efficient cultivation

Язык: Английский

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Fungal Endophytes: An Alternative Biocontrol Agent against Phytopathogenic Fungi

Encyclopedia, Год журнала: 2023, Номер 3(2), С. 759 - 780

Опубликована: Июнь 19, 2023

There has been renewed interest in the application of endophytic fungi to control phytopathogenic fungi, which cause significant damage crop health, ultimately leading losses agricultural productivity. Endophytic inhibit pathogens via different modes action—mycoparasitism, competition (for nutrients and ecological niches), antibiosis, induction plant defense—thus demonstrating ability a wide range growth phases habitats. However, many studies have conducted under laboratory conditions, there is huge lack real field testing was performed. Aspergillus, Clonostachys, Coniothyrium, Trichoderma, Verticillium proven be most effective fungal biocontrol agents. Trichoderma regarded as promising group commercial formulations. In this study, we attempted emphasize significance endophytes controlling while reporting recent advances biology application.

Язык: Английский

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The medicinal plant Berberis aristata and its endophytes for pharmacological applications: Current research and future challenges

Journal of Applied Biology & Biotechnology, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Exploring medicinal plants for their natural products to benefit humanity has been a practice since ancient times. Berberis aristata, also known as tree turmeric or Indian barberry, dominant spot in conventional medicine 2500 plus years. In the latter years, this particular species gained prime attention pharmaceutical zone due its scarcity. The plant and formulations are used treat numerous human health threats including cancer, diabetes, diarrhea, wound relieving, infections of eyes ears, gynecological disorders, HIV-AIDS. bioactive compounds present B. aristata found contain anti-inflammatory, antioxidant, anti-microbial properties. is critically jeopardized Himalaya collection roots at large scale obtaining berberine alkaloid conservation great concern. At present, isolation endophytic entities from remedial revolutionized path discovering drugs plants. Endophytes can produce same host well another address variety issues. review examines bioprospecting shrub family Berberidaceae, associated endophytes production with application health. Finally, provides comprehensive outlook on current status future prospects combat an exciting area research that should be further explored.

Язык: Английский

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Why Are There So Few Basidiomycota and Basal Fungi as Endophytes? A Review

Journal of Fungi, Год журнала: 2024, Номер 10(1), С. 67 - 67

Опубликована: Янв. 15, 2024

A review of selected studies on fungal endophytes confirms the paucity Basidiomycota and basal fungi, with almost 90% attributed to Ascomycota. Reasons for low number including Chytridiomycota, Mucoromycota, Mortierellomycota, are advanced, isolation procedure media, incubation period slow growth basidiomycetes, identification non-sporulating isolates, endophyte competition, fungus–host interactions. We compare detection through culture-dependent methods culture-independent methods, role fungi senescence host plant, next-generation studies.

Язык: Английский

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Community Structure and Diversity of Endophytic Fungi in Cultivated Polygala crotalarioides at Two Different Growth Stages Based on Culture-Independent and Culture-Based Methods

Journal of Fungi, Год журнала: 2024, Номер 10(3), С. 195 - 195

Опубликована: Март 4, 2024

Polygala crotalarioides, a perennial herbaceous plant found in southwest China, has the potential to be used treatment of Alzheimer’s disease. Endophytic fungi that reside within medicinal herbs play an important ecological role their host plants and can serve as valuable source for identifying active components. However, little is known about diversity, structure endophytic P. crotalarioides. In this study, we investigated community diversity leaves, stems, roots crotalarioides at both 1- 2-year-growth stages using modern culture-independent method (high-throughput sequencing, HTS) culture-based methods. Using HTS, our results revealed richness varied depending on organs growth stages. Specifically, stems leaves exhibited significantly higher compared roots. Additionally, highest was observed 2-year-old plants. At genus level, Fusarium, Colletotrichum, Phoma were most abundant 1-year-old samples, while Cercospora, Apiotrichum, Fusarium prevalent samples. A total 55 fungal strains belonging two phyla 24 genera isolated from 150 tissue segments The anti-acetylcholinesterase activity these isolates evaluated vitro five them, Phialophora mustea PCAM010, Diaporthe nobilis PCBM027, oxysporum LP41, F. SR60, herbarum SM81, showed strong (>50% inhibition rate). These findings will theoretical basis practical guide comprehending structural composition, biological bioactivity

Язык: Английский

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Bioactive Compounds Produced by Endophytic Microorganisms Associated with Bryophytes—The “Bryendophytes”

Molecules, Год журнала: 2023, Номер 28(7), С. 3246 - 3246

Опубликована: Апрель 5, 2023

The mutualistic coexistence between the host and endophyte is diverse complex, including growth regulation, exchange of substances like nutrients or biostimulants, protection from microbial herbivore attack. latter commonly associated with production by endophytes bioactive natural products, which also possess multiple activities, antibacterial, insecticidal, antioxidant, antitumor, antidiabetic properties, making them interesting valuable model for future development into drugs. higher plants have been extensively studied, but there a dearth information on biodiversity endophytic microorganisms bryophytes and, more importantly, their metabolites. For first time, we name bryophyte “bryendophytes” to elaborate this important productive source biota. In review, summarize current knowledge diversity compounds produced endophytes, emphasizing molecules bryendophytes. Moreover, isolation methods bryendophytes mosses, liverworts, hornworts are described.

Язык: Английский

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Endophytic fungi hosted anti-diabetic medicinal plants as a source of α-Amylase and α-Glucosidase inhibitors

Microbial Biosystems, Год журнала: 2024, Номер 8(2), С. 44 - 56

Опубликована: Янв. 28, 2024

Diabetes mellitus (DM) is a chronic disease that occurs when the body cannot produce or use insulin properly.It considered one of most prevalent and fastest-growing diseases in world, expected to affect 693 million adults by 2045.DM can cause mortalities morbidities, although this not directly caused diabetes, persistent hyperglycemia lead serious physical consequences such as blindness, kidney failure, heart disease, peripheral nerve damage.Treatment these complications includes removing main symptoms maintaining good glycemic control.DM continues put economic health pressures on individuals countries affects quality life patients.It's miles crucial search for newer more effective antidiabetic agents with fewer adverse effects cheaper costs minimize current future burden diabetes.Recently, endophytic fungi have received great deal attention due their ability secondary metabolites like those host plant.This provides an opportunity cheaply therapeutic natural compounds overcome diabetes.Recent research has focused thoroughly understanding role endophytes impact diabetes.They showed promising results paved way extract find new bioactive substances anti-diabetic properties.The review focuses fungi-hosted medicinal plants source α-Amylase α-Glucosidase inhibitors.

Язык: Английский

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Endophytic fungi in buckwheat seeds: exploring links with flavonoid accumulation

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Фев. 20, 2024

Buckwheat is a famous edible and medicinal coarse cereal which contain abundant of bioactive flavonoids, such as rutin. In this study, the composition diversity endophytic fungi in eight different buckwheat seeds were analyzed by high-throughput sequencing ITS rDNA. Results showed that, fungal sequences reads allocated to 272 OTUs, them, 49 OTUs shared seeds. These could be classified into 6 phyla, 19 classes, 41 orders, 79 families, 119 genera, 191 species. At genus level, Alternaria sp. was domain endophyte. Besides, endophytes belonged genera Epicocum, Cladosporium, Botrytis, Filbobasidium, Stemphylium , Vishniacozyma highly The total flavonoids rutin contents tartary cultivars (CQ, XQ, CH, K2) much higher than those common (HT, T2, T4, T8). For cultivars, ranging from 2.6% 3.3% 0.9% 1.3%, respectively. Accordingly, samples displayed stronger antioxidant activity buckwheat. Spearman correlation heat map analysis successfully found that certain species Alternaria, Botryosphaeria, Colletorichum Diymella exhibited significant positive with contents. study preliminary revealed fungi-plant interaction relationship at secondary metabolite provide novel strategy for increasing accumulation seeds, well improving their quality.

Язык: Английский

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Rooting for growth: Meta-analyzing the role of Endophytic fungi in plant growth

Scientia Horticulturae, Год журнала: 2024, Номер 333, С. 113276 - 113276

Опубликована: Май 7, 2024

Язык: Английский

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