Variation in Columbin Content in Rhizome of different Tinospora sagittata Varieties DOI Creative Commons

Yonghui Sang,

Mohammad Murtaza Alami, Huaijun Si

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 21, 2024

Abstract Columbin, a key secondary metabolite in Tinospora sagittata, exhibits diverse pharmacological properties, yet its content shows notable variation across different varieties. This study investigated the molecular and biochemical basis behind such variation, analyzing columbin 32 T. sagittata varieties ranging from 5.40 mg/g to 46.09 mg/g. High low-columbin-content were selected for in-depth transcriptomic untargeted metabolomic analyses. Differential expression patterns of genes metabolites between leaves rhizomes these explored. Results revealed that involved biosynthesis pathway, namely, DXS, DXR, MCS, GPPS, PMK, exhibited significantly higher high At same time, HMGR HDR levels notably lower. Furthermore, columbin-content contained increased isoprene levels, crucial precursor. These findings suggest gene precursor variations likely drive differences insight into clarifies provides valuable direction breeding cultivating varieties, amplifying potential therapeutic applications.

Язык: Английский

The Role of Polyphenols in Abiotic Stress Tolerance and Their Antioxidant Properties to Scavenge Reactive Oxygen Species and Free Radicals DOI Creative Commons
Muhammad Junaid Rao, Bingsong Zheng

Antioxidants, Год журнала: 2025, Номер 14(1), С. 74 - 74

Опубликована: Янв. 10, 2025

Plants have evolved complex mechanisms to cope with diverse abiotic stresses, the phenylpropanoid pathway playing a central role in stress adaptation. This produces an array of secondary metabolites, particularly polyphenols, which serve multiple functions plant growth, development, regulating cellular processes, and responses. Recent advances understanding molecular underlying metabolism revealed regulatory networks involving MYB transcription factors as master regulators their interactions signaling pathways. review summarizes our current polyphenol-mediated adaptations plants, emphasizing regulation function key compounds. We discussed how various including heat chilling stress, drought, salinity, light UV radiation, nanoparticles chemical heavy metal toxicity, modulate trigger accumulation specific polyphenolic The antioxidant properties these phenolic acids, flavonoids, anthocyanins, lignin, roles reactive oxygen species scavenging, neutralizing free radicals, membrane stabilization, osmotic adjustment are discussed. Understanding metabolic responses is crucial for developing stress-resilient crops improving agricultural productivity under increasingly challenging environmental conditions. provides comprehensive insights into integrating adaptation mechanisms, highlighting potential targets enhancing crop tolerance through adjustment.

Язык: Английский

Процитировано

1
Dynamic Changes in Functional Components of Dendrobium officinale and Their Applications in Food Science: A Review DOI
Mei Wang,

Gaige Shao,

Minquan Song

и другие.

Plant Foods for Human Nutrition, Год журнала: 2025, Номер 80(1)

Опубликована: Фев. 10, 2025

Язык: Английский

Процитировано

0
Identification of α-Glucosidase Inhibitors and Assessment of Human Safety from 491 Native Plant Species of Jeju Island DOI Creative Commons

Ho‐Min Kang,

Jin-Kyu Kang,

Pomjoo Lee

и другие.

Cosmetics, Год журнала: 2025, Номер 12(2), С. 40 - 40

Опубликована: Фев. 28, 2025

In contemporary society, obesity has become a critical public health issue driven by lifestyle modifications such as unhealthy dietary patterns, physical inactivity, and aging. Beyond its aesthetic implications, is significant etiological factor for metabolic disorders, including type 2 diabetes cardiovascular diseases. With increasing emphasis on weight management holistic health, the demand slimming cosmetics risen substantially. particular, formulation of natural-origin cosmetic ingredients that facilitate lipolysis improve dermal elasticity being actively investigated, presenting compelling balance safety efficacy. This study examined bioactivities 570 plant extracts sourced from Jeju Island to evaluate their potential raw materials. The α-glucosidase inhibitory activity was analyzed determine antidiabetic potential. Among tested extracts, 18 species, Rumex japonicus (IC50, 0.93 ± 0.21 μg/mL), Rhynchosia volubilis 1.2 0.16 Arachniodes standishii 4.88 0.89 exhibited substantially lower IC50 values compared positive control, acarbose 251.17 22.67 μg/mL). These findings highlight these innovative inhibitors, potentially serving alternatives conventional pharmacological agents. Additionally, topical application assessed via cytotoxicity assays using human keratinocyte (HaCaT) cells primary skin irritation tests. results indicated no in HaCaT evidence at concentrations deemed non-toxic cells. summary, exhibit promise multifunctional ingredients, combining properties through adipogenesis inhibition with skin-enhancing benefits.

Язык: Английский

Процитировано

0
Insight into endophytic microbiota-driven geographical and bioactive signatures toward a novel quality assessment model for Codonopsis Radix DOI
Yingjie Liu, Yuqing Niu,

Zhongkun Zhou

и другие.

Plant Physiology and Biochemistry, Год журнала: 2025, Номер unknown, С. 109888 - 109888

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Comparative Metabolic Profiling and Biological Evaluation of Essential Oils from Conocarpus Species: Antidiabetic, Antioxidant, and Antimicrobial Potential DOI Creative Commons

Aya Salim,

Alya Ali Arasteh,

Ruqaiya Sahrish

и другие.

Plants, Год журнала: 2025, Номер 14(3), С. 464 - 464

Опубликована: Фев. 5, 2025

Essential oils (EOs) are a diverse source of bioactive compounds with remarkable therapeutic potential. Despite their significance, Conocarpus EOs have been largely underexplored. This study provides novel comparison the metabolic profiles and biological activities from C. lancifolius, erectus green, silver leaves cultivated in United Arab Emirates (UAE), offering unique insights into distinct properties potential applications. were extracted via hydro-distillation, analyzed using gas chromatography–mass spectrometry (GC-MS), subjected to chemometric analysis. Their antioxidant (2,2-diphenyl-1-picrylhydrazyl (DPPH) ferric reducing ability plasma (FRAP) assays), antidiabetic (α-amylase α-glucosidase inhibition), acetylcholinesterase (AChE) inhibition antimicrobial assessed. A total 92 metabolites identified, heptacosane nonacosane as key species discriminants. lancifolius EO showed strongest α-amylase (IC50 8.75 ± 0.54 µg/mL) 22.31 0.92 inhibitory activities, while demonstrated superior capacity 349.78 8.26 µg/mL, DPPH assay). exhibited best activity, particularly against Staphylococcus aureus (MIC 625 µg/mL). inhibited E. coli albicans In contrast, no activity Aspergillus niger. These findings highlight antioxidants for managing diabetes that may be utilized either nutraceuticals, dietary supplements or even pharmaceutical formulations. Moreover, owing significant added medical disinfectants several products. However, further, vivo validation exploration is still needed.

Язык: Английский

Процитировано

0
Mining the Candidate Transcription Factors Modulating Tanshinones’ and Phenolic Acids’ Biosynthesis Under Low Nitrogen Stress in Salvia miltiorrhiza DOI Open Access
Yating Cheng,

Siqi Gui,

Siyu Hao

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1774 - 1774

Опубликована: Фев. 19, 2025

Mining valuable genes is helpful to breed high-quality Salvia miltiorrhiza exhibiting efficient nitrogen fertilizer utilization efficiency. In the present study, transcriptome sequencing was introduced select candidate transcription factors (TFs) involved in tanshinones' (TAs) and phenolic acids' (PHAs) biosynthesis as well low (LN) stress. totally, 97.71 Gb clean data obtained from fifteen samples 30,975 unigenes were assembled. Among of them, 27,843 successfully annotated. Overall, 8663 differential expression (DEGs) identified, among which 5034 up-regulated, 3629 down-regulated. By enrichment DEGs together with gene co-expression network construction, 10 TFs including HSFB2b, LBD12, ERF1A, ERF98, LBD25, HSF24, RAM1, HSFA4B, TCP8, WRKY24 finally retrieved, are predicted participate modulating TA PHA under LN Quantitative real-time polymerase chain reaction (qRT-PCR) detection further detect profile These findings offer a resource for in-depth study TAs 'and PHAs' stress S. miltiorrhiza.

Язык: Английский

Процитировано

0
Phytochemical Profile, Antioxidant and Antimicrobial Activity of Two Species of Oak: Quercus sartorii and Quercus rysophylla DOI Creative Commons

Elizabeth Coyotl-Martinez,

Juan Augusto Hernández-Rivera,

José L. Arturo Parra-Suarez

и другие.

Applied Biosciences, Год журнала: 2025, Номер 4(1), С. 13 - 13

Опубликована: Март 4, 2025

The genus Quercus (Fagaceae) is one of the most widely distributed and species-diverse trees in Northern Hemisphere. present study addresses investigation phyto-chemical profile by ten assays, antioxidant activity scavenging DPPH radicals, total phenolic content, flavonoids, antimicrobial against three pathogenic bacteria with foliage two species red oak (Quercus sartorii rysophylla). Both showed a high content aqueous extract (22,342.10 ± 3076.5 mg GAE/kg plant 17,747.14 1139.9 plant, respectively). In flavonoid Q. higher amount ethanolic (24,587.42 996.3 QE/kg plant), while for rysophylla, it was methanolic (19,875.66 2754.01 plant). radical activity, highest percentage inhibition (81.14 1.7%), (82.60 2.7%). tests, halos were obtained strains Acinetobacter baumannii Staphylococcus aureus both species. All this gives guideline to comprehensively elucidate metabolites these further application dispute or diseases related imbalance reactive oxygen (ROS).

Язык: Английский

Процитировано

0
Antioxidant profiling and quality assessment of Lithocarpus polystachyus sweet tea using LC-ECD and LC-MS/MS DOI Creative Commons

Jinfen Zheng,

Wei Pan,

M.J. Liu

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 16, 2025

Abstract This study used liquid chromatography-electrochemical detection (LC-ECD) combined with chromatography-tandem mass spectrometry (LC-MS/MS) to screen and characterize the antioxidant components of sweet tea ( Lithocarpus polystachyus ), evaluate quality samples from different origins. First, total phenolic flavonoid content tea, as well in vitro activity, including ABTS DPPH radical scavenging capacities ferric reducing ability, were determined. Samples origins demonstrated potent correlation coefficients exceeding 0.8 between activities. Then, fingerprints generated by LC-ECD 22 common peaks identified LC-MS/MS, most them being phloretin derivatives. The clustering heat map revealed differences various regions, major compounds trilobatin, phlorizin, 3-hydroxyphlorizin, its isomer. Furthermore, grey relational analysis confirmed a strong association (protocatechuic acid, epicatechin, isoquercitrin, trilobatin) trilobatin showing highest contribution, reaching 0.9. Finally, 11 quantitatively screened using providing guidance for control.

Язык: Английский

Процитировано

0
Variation in Columbin Content in Rhizome of different Tinospora sagittata Varieties DOI Creative Commons

Yonghui Sang,

Mohammad Murtaza Alami, Huaijun Si

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 21, 2024

Abstract Columbin, a key secondary metabolite in Tinospora sagittata, exhibits diverse pharmacological properties, yet its content shows notable variation across different varieties. This study investigated the molecular and biochemical basis behind such variation, analyzing columbin 32 T. sagittata varieties ranging from 5.40 mg/g to 46.09 mg/g. High low-columbin-content were selected for in-depth transcriptomic untargeted metabolomic analyses. Differential expression patterns of genes metabolites between leaves rhizomes these explored. Results revealed that involved biosynthesis pathway, namely, DXS, DXR, MCS, GPPS, PMK, exhibited significantly higher high At same time, HMGR HDR levels notably lower. Furthermore, columbin-content contained increased isoprene levels, crucial precursor. These findings suggest gene precursor variations likely drive differences insight into clarifies provides valuable direction breeding cultivating varieties, amplifying potential therapeutic applications.

Язык: Английский

Процитировано

0