Improvement of cell growth in green algae Chlamydomonas reinhardtii through co-cultivation with yeast Saccharomyces cerevisiae
Biotechnology Letters,
Год журнала:
2024,
Номер
46(3), С. 431 - 441
Опубликована: Апрель 5, 2024
Язык: Английский
Co-utilization of microalgae and heterotrophic microorganisms improves wastewater treatment efficiency
Applied Microbiology and Biotechnology,
Год журнала:
2024,
Номер
108(1)
Опубликована: Сен. 18, 2024
Wastewater
treatment
using
the
activated
sludge
method
requires
a
large
amount
of
electricity
for
aeration.
Therefore,
wastewater
co-culture
systems
microalgae
and
heterotrophic
microorganisms,
which
do
not
require
aeration,
has
attracted
attention
as
an
energy-saving
alternative
to
method.
In
this
study,
we
investigated
different
combinations
microorganisms
improve
efficiency
treatment.
Three
types
five
were
used
in
combination
The
Chlamydomonas
reinhardtii
NIES-2238
Saccharomyces
cerevisiae
SH-4
showed
highest
efficiency.
Using
artificial
treatment,
removal
rates
total
organic
carbon,
PO4
Язык: Английский
Co-culture systems of microalgae and heterotrophic microorganisms: applications in bioproduction and wastewater treatment and elucidation of mutualistic interactions
World Journal of Microbiology and Biotechnology,
Год журнала:
2024,
Номер
40(11)
Опубликована: Окт. 26, 2024
Язык: Английский
Co-utilization of microalgae and heterotrophic microorganisms improves wastewater treatment efficiency
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 6, 2024
Abstract
Wastewater
treatment
using
co-culture
systems
of
microalgae
and
heterotrophic
microorganisms
is
expected
to
be
useful
under
atmospheric
dilute
carbon
dioxide
conditions.
In
this
study,
we
investigated
the
combination
improve
efficiency
wastewater
treatment.
Furthermore,
elucidate
cause
changes
in
system,
gene
expression
were
revealed
through
transcriptome
analysis.
Three
types
five
used
for
The
Chlamydomonas
reinhardtii
NIES-2238
Saccharomyces
cerevisiae
SH-4
showed
highest
efficiency.
Using
artificial
treatment,
removal
rates
TOC
(Total
organic
carbon),
PO4
3-
,
NH
4
+
reached
80%,
93%,
63%,
respectively,
after
18
h
Transcriptome
analysis
that
combined
altered
1371
692
genes
C.
S.
respectively.
upregulated
included
those
related
molecular
ion
transport.
Genes
cell
protection
from
various
damage
stress.
To
best
our
knowledge,
first
study
show
a
green
algae
yeast
improves
As
both
alga
are
highly
safe
microorganisms,
establishment
their
effective
significant.
Язык: Английский
Improvement of D-lactic acid production from methanol by metabolically engineeredKomagataella phaffiivia ultra-violet mutagenesis
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 15, 2024
Abstract
Methanol
has
attracted
attention
as
an
alternative
carbon
source
to
petroleum.
Komagataella
phaffii
,
a
methanol-assimilating
yeast,
is
useful
host
for
the
chemical
production
from
methanol.
A
previous
study
successfully
constructed
metabolically
engineered
K.
GS115/S8/Z3
strain
capable
of
producing
D-lactic
acid
In
this
study,
we
aimed
develop
with
improved
by
applying
ultra-violet
mutagenesis
acid-producing
strain,
GS115/S8/Z3.
The
resulting
mutant
DLac_Mut2_221
produced
5.38
g/L
methanol,
1.52-fold
increase
compared
parent
Transcriptome
analysis
revealed
167
differentially
expressed
genes
in
DLac_Mut2_221,
comprising
104
upregulated
and
63
downregulated
genes.
These
results
suggest
that
improvement
methanol
involves
three
important
mechanisms:
(1)
avoiding
excessive
formaldehyde
accumulation,
(2)
activating
glyoxylate
pathway,
(3)
reducing
expression
O
-glycosylated
proteins
required
cell
wall
stability.
Metabolic
engineering
strategies
based
on
knowledge
gained
will
contribute
improving
productivity
various
chemicals
Язык: Английский