Improvement of D-lactic acid production from methanol by metabolically engineeredKomagataella phaffiivia ultra-violet mutagenesis DOI Creative Commons

Yoshifumi Inoue,

K Nakamura, Ryosuke Yamada

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 15, 2024

Abstract Methanol has attracted attention as an alternative carbon source to petroleum. Komagataella phaffii , a methanol-assimilating yeast, is useful host for the chemical production from methanol. A previous study successfully constructed metabolically engineered K. GS115/S8/Z3 strain capable of producing D-lactic acid In this study, we aimed develop with improved by applying ultra-violet mutagenesis acid-producing strain, GS115/S8/Z3. The resulting mutant DLac_Mut2_221 produced 5.38 g/L methanol, 1.52-fold increase compared parent Transcriptome analysis revealed 167 differentially expressed genes in DLac_Mut2_221, comprising 104 upregulated and 63 downregulated genes. These results suggest that improvement methanol involves three important mechanisms: (1) avoiding excessive formaldehyde accumulation, (2) activating glyoxylate pathway, (3) reducing expression O -glycosylated proteins required cell wall stability. Metabolic engineering strategies based on knowledge gained will contribute improving productivity various chemicals

Language: Английский

Improvement of cell growth in green algae Chlamydomonas reinhardtii through co-cultivation with yeast Saccharomyces cerevisiae DOI

Yukino Karitani,

Ryosuke Yamada, Takuya Matsumoto

et al.

Biotechnology Letters, Journal Year: 2024, Volume and Issue: 46(3), P. 431 - 441

Published: April 5, 2024

Language: Английский

Citations

4
Co-utilization of microalgae and heterotrophic microorganisms improves wastewater treatment efficiency DOI Creative Commons

Miiku Takahashi,

Yukino Karitani,

Ryosuke Yamada

et al.

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: Sept. 18, 2024

Wastewater treatment using the activated sludge method requires a large amount of electricity for aeration. Therefore, wastewater co-culture systems microalgae and heterotrophic microorganisms, which do not require aeration, has attracted attention as an energy-saving alternative to method. In this study, we investigated different combinations microorganisms improve efficiency treatment. Three types five were used in combination The Chlamydomonas reinhardtii NIES-2238 Saccharomyces cerevisiae SH-4 showed highest efficiency. Using artificial treatment, removal rates total organic carbon, PO4

Language: Английский

Citations

1
Co-culture systems of microalgae and heterotrophic microorganisms: applications in bioproduction and wastewater treatment and elucidation of mutualistic interactions DOI

Miiku Takahashi,

Ryosuke Yamada, Takuya Matsumoto

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 40(11)

Published: Oct. 26, 2024

Language: Английский

Citations

1
Co-utilization of microalgae and heterotrophic microorganisms improves wastewater treatment efficiency DOI Creative Commons

Miiku Takahashi,

Yukino Karitani,

Ryosuke Yamada

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 6, 2024

Abstract Wastewater treatment using co-culture systems of microalgae and heterotrophic microorganisms is expected to be useful under atmospheric dilute carbon dioxide conditions. In this study, we investigated the combination improve efficiency wastewater treatment. Furthermore, elucidate cause changes in system, gene expression were revealed through transcriptome analysis. Three types five used for The Chlamydomonas reinhardtii NIES-2238 Saccharomyces cerevisiae SH-4 showed highest efficiency. Using artificial treatment, removal rates TOC (Total organic carbon), PO4 3- , NH 4 + reached 80%, 93%, 63%, respectively, after 18 h Transcriptome analysis that combined altered 1371 692 genes C. S. respectively. upregulated included those related molecular ion transport. Genes cell protection from various damage stress. To best our knowledge, first study show a green algae yeast improves As both alga are highly safe microorganisms, establishment their effective significant.

Language: Английский

Citations

0
Improvement of D-lactic acid production from methanol by metabolically engineeredKomagataella phaffiivia ultra-violet mutagenesis DOI Creative Commons

Yoshifumi Inoue,

K Nakamura, Ryosuke Yamada

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 15, 2024

Abstract Methanol has attracted attention as an alternative carbon source to petroleum. Komagataella phaffii , a methanol-assimilating yeast, is useful host for the chemical production from methanol. A previous study successfully constructed metabolically engineered K. GS115/S8/Z3 strain capable of producing D-lactic acid In this study, we aimed develop with improved by applying ultra-violet mutagenesis acid-producing strain, GS115/S8/Z3. The resulting mutant DLac_Mut2_221 produced 5.38 g/L methanol, 1.52-fold increase compared parent Transcriptome analysis revealed 167 differentially expressed genes in DLac_Mut2_221, comprising 104 upregulated and 63 downregulated genes. These results suggest that improvement methanol involves three important mechanisms: (1) avoiding excessive formaldehyde accumulation, (2) activating glyoxylate pathway, (3) reducing expression O -glycosylated proteins required cell wall stability. Metabolic engineering strategies based on knowledge gained will contribute improving productivity various chemicals

Language: Английский

Citations

0