Pathway engineering facilitates efficient protein expression in Pichia pastoris

Applied Microbiology and Biotechnology, Год журнала: 2022, Номер 106(18), С. 5893 - 5912

Опубликована: Авг. 30, 2022

Язык: Английский

---

Heat-Shock Proteins in Leukemia and Lymphoma: Multitargets for Innovative Therapeutic Approaches

Cancers, Год журнала: 2023, Номер 15(3), С. 984 - 984

Опубликована: Фев. 3, 2023

Heat-shock proteins (HSPs) are powerful chaperones that provide support for cellular functions under stress conditions but also the homeostasis of basic machinery. All cancer cells strongly rely on HSPs, as they must continuously adapt to internal microenvironmental stresses survive. In solid tumors, HSPs have been described helping correct folding misfolded proteins, sustain oncogenic pathways, and prevent apoptosis. Leukemias lymphomas overexpress which frequently associated with resistance therapy. therefore proposed new therapeutic targets. Given specific biology hematological malignancies, it is essential revise their role in this field, providing a more adaptable comprehensive picture would help design future clinical trials. To end, review will describe different pathways regulated by HSP27, HSP70, HSP90, and, not least, HSP110 leukemias lymphomas.

Язык: Английский

---

Heat shock protein 90 inhibitor RGRN-305 potently attenuates skin inflammation

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Фев. 7, 2023

Chronic inflammatory skin diseases may have a profound negative impact on the quality of life. Current treatment options be inadequate, offering an unsatisfactory response or side effects. Therefore, ongoing efforts exist to identify novel effective and safe treatments. Heat shock protein (HSP) 90 is chaperone that promotes activity wide range client proteins including key proinflammatory molecules involved in aberrant inflammation. Recently, proof-of-concept clinical trial 13 patients suggested RGRN-305 (an HSP90 inhibitor) oral for psoriasis. However, inhibition therapeutic approach extending beyond psoriasis include multiple immune-mediated diseases.This study aimed investigate (i) anti-inflammatory effects mechanisms (ii) feasibility topical administration (new route administration) models inflammation elicited by 12-O-tetradecanoylphorbol-13-acetate (TPA) primary human keratinocytes mice (irritative dermatitis murine model).In stimulated with TPA, Nanostring® nCounter gene expression assay demonstrated suppressed many genes. Furthermore, when measured quantitative real-time polymerase chain reaction (RT-qPCR), significantly reduced TNF, IL1B, IL6 CXCL8. We next application ameliorated TPA-induced mice. The increase ear thickness (a marker inflammation) was (up 89% inhibition). In accordance, RT-qPCR tissue robustly markers (Tnf, Il1b, Il6, Il17A Defb4). Moreover, RNA sequencing revealed mitigated alterations genes implicated Lastly, we discovered were mediated, at least partly, suppressing NF-κB, ERK1/2, p38 MAPK c-Jun signaling pathways, which are consistent previous findings other experimental summary, targeting cytokines pathways. Our suggest mechanism action treating disease psoriasis, it option.

Язык: Английский

---

Trichinella spiralis as a Potential Antitumor Agent: An Update

World s Veterinary Journal, Год журнала: 2023, Номер 13, С. 65 - 74

Опубликована: Март 25, 2023

Due to the limited success of therapeutic strategies in treating tumors, a new practical potent approach is needed. This review aimed investigate previous literature related tumors and Trichinella spiralis (T. spiralis). In recent years, there has been growing interest utilizing biological, viral, bacterial, yeast, parasitic agents cure cancers. According several studies, some parasites could interferee with tumors’ growth. There much discussion about parasites’ applications animals humans. T. was found have antitumor properties. The active proteins spiralis, such as Caveolin-1, Heat shock proteins, Ribosomal are thought inhibit growth cancers, melanoma, myeloma, sarcoma, leukemia, stomach cancer, colon breast lung cancer. addition, these induce apoptosis specific neoplastic cells. Accordingly, antigens derived from may be helpful cancer immunotherapy. However, still many unanswered questions regarding spiralis’ potential use biotherapy agent against Future studies should focus on purification parasite their for wider-scale trials animal models.

Язык: Английский

---

Oxidative Stress Biomarkers in Male Infertility: Established Methodologies and Future Perspectives

Genes, Год журнала: 2024, Номер 15(5), С. 539 - 539

Опубликована: Апрель 25, 2024

Male fertility can be affected by oxidative stress (OS), which occurs when an imbalance between the production of reactive oxygen species (ROS) and body's ability to neutralize them arises. OS damage cells influence sperm production. High levels lipid peroxidation have been linked reduced motility decreased fertilization ability. This literature review discusses most commonly used biomarkers measure caused ROS, such as high level in seminal plasma indicator antioxidant activity. The investigated include 8-hydroxy-2-deoxyguanosine acid (8-OHdG), a marker DNA F2 isoprostanoids (8-isoprostanes) produced peroxidation. Furthermore, this focuses on recent methodologies including NGS polymorphisms differentially expressed gene (DEG) analysis, well epigenetic mechanisms ROS during spermatogenesis along with new developed evaluate biomarkers. Finally, addresses valuable insight into male infertility provided these advances how they led treatment possibilities. Overall, use has supplied innovative diagnostic therapeutic approaches, enhancing our understanding mechanisms.

Язык: Английский

---

Protective effects of butyric acid during heat stress on the survival, immune response, histopathology, and gene expression in the hepatopancreas of juvenile pacific shrimp (L. Vannamei)

Fish & Shellfish Immunology, Год журнала: 2024, Номер 150, С. 109610 - 109610

Опубликована: Май 9, 2024

Язык: Английский

---

Combination therapy involving HSP90 inhibitors for combating cancer: an overview of clinical and preclinical progress

Archives of Pharmacal Research, Год журнала: 2024, Номер 47(5), С. 442 - 464

Опубликована: Апрель 17, 2024

Язык: Английский

---

Heat Shock Protein 90 as Therapeutic Target for CVDs and Heart Ageing

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(2), С. 649 - 649

Опубликована: Янв. 7, 2022

Cardiovascular diseases (CVDs) are the leading cause of death globally, representing approximately 32% all deaths worldwide. Molecular chaperones involved in heart protection against stresses and age-mediated accumulation toxic misfolded proteins by regulation protein synthesis/degradation balance refolding proteins, thus supporting high metabolic demand cells. Heat shock 90 (HSP90) is one main cardioprotective chaperones, represented cytosolic HSP90a HSP90b, mitochondrial TRAP1 ER-localised Grp94 isoforms. Currently, way to study functional role HSPs application HSP inhibitors, which could have a different action. In this review, we discussed recently investigated HSP90 cardioprotection, atherosclerosis, CVDs development involvements clients activation molecular pathways signalling mechanisms, related ageing.

Язык: Английский

---

Valosin containing protein (VCP): initiator, modifier, and potential drug target for neurodegenerative diseases

Molecular Neurodegeneration, Год журнала: 2023, Номер 18(1)

Опубликована: Авг. 7, 2023

Abstract The AAA + ATPase valosin containing protein (VCP) is essential for cell and organ homeostasis, especially in cells of the nervous system. As part a large network, VCP collaborates with many cofactors to ensure proteostasis under normal, stress, disease conditions. A number mutations have revealed importance human health. In particular, facilitates dismantling aggregates removal dysfunctional organelles. These are critical events prevent malfunction brain other parts line this idea, mutants linked onset progression neurodegeneration diseases. intricate molecular mechanisms that connect distinct pathologies continue be uncovered. Emerging evidence supports model controls cellular functions on multiple levels type specific fashion. Accordingly, derail homeostasis through several can instigate disease. Our review focuses association between neurodegeneration. We discuss latest insights field, emphasize open questions, speculate potential as drug target some most devastating forms

Язык: Английский

---

HSP70 and Primary Arterial Hypertension

Biomolecules, Год журнала: 2023, Номер 13(2), С. 272 - 272

Опубликована: Фев. 1, 2023

Heat shock protein 70 (HSP70) production is a stress-generated cellular response with high interspecies homology. HSP70 has both chaperone and cytokine functions may induce, depending on the context, tolerogenic anti-inflammatory reactivity or immunogenic autoimmune reactivity. Intracellular (chaperoning transit of antigens to MHC in antigen-presenting cells) extracellular HSP70-related effects are associated hypertension, which an inflammatory condition recognized as most important risk factor for cardiovascular disease mortality. Here, we review (a) relationship between HSP70, inflammation immune reactivity, (b) clinical evidence relating stress, anti-HSP70 primary hypertension (c) experimental data showing that salt-sensitive delayed hypersensitivity HSP70. This consequence kidneys be prevented corrected by T-cell-driven inhibition kidney triggered specific epitopes Finally, discuss our postulate lifelong stress signals danger-associated molecular patterns stimulate HSP-70 individual genetic epigenetic characteristics determine whether would drive causing or, alternatively, immunomodulatory responses protect against hypertension.

Язык: Английский

---