Lebanese Cannabis sativa L. extract protects from cisplatin-induced nephrotoxicity in mice by inhibiting podocytes apoptosis
Journal of Cannabis Research,
Год журнала:
2025,
Номер
7(1)
Опубликована: Янв. 16, 2025
Язык: Английский
The renoprotective effects of cannabidiol on lipopolysaccharide-induced systemic inflammation model of rats
Naunyn-Schmiedeberg s Archives of Pharmacology,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 24, 2024
Язык: Английский
Cannabidiol mitigates methotrexate-induced hepatic injury via SIRT-1/p53 signaling and mitochondrial pathways: reduces oxidative stress and inflammation
Drug and Chemical Toxicology,
Год журнала:
2024,
Номер
unknown, С. 1 - 9
Опубликована: Ноя. 27, 2024
Methotrexate
(MTX),
a
widely
used
chemotherapeutic
agent,
often
induces
hepatotoxicity,
limiting
its
clinical
utility.
Cannabidiol
(CBD),
derived
from
hemp,
possesses
antioxidant,
anti-inflammatory,
and
antiapoptotic
properties.
This
study
aims
to
investigate
CBD's
protective
effects
against
MTX-induced
liver
injury
elucidate
the
underlying
mechanisms.
Thirty-two
female
Wistar
Albino
rats
were
divided
into
four
groups:
control,
MTX
(20
mg/kg
intraperitoneally
[i.p.]
once),
MTX+CBD
i.p.
once
+
5
for
seven
days),
CBD
(5
mg/kg,
days).
Biochemical
analyses
of
serum
tissues
performed
assess
oxidative
stress
markers
(total
oxidant
status,
total
antioxidant
index),
function
tests
(AST,
ALT),
enzyme
activities
(glutathione
peroxidase,
superoxide
dismutase).
Histopathological
immunohistochemical
examinations
conducted
evaluate
tissue
damage
TNF-α
expression.
Genetic
measure
expression
levels
SIRT-1,
p53,
Bcl-2,
Bax
genes
using
RT-qPCR.
administration
increased
markers,
enzymes,
TNF-α,
while
decreasing
defenses
SIRT-1
reversed
these
alterations
effectively.
mitigated
hepatotoxicity
by
reducing
stress,
inflammation,
apoptosis.
It
activates
via
upregulation,
suppresses
inflammation
prevents
apoptosis
modulating
gene
expressions.
These
findings
suggest
could
be
promising
therapeutic
agent
chemotherapy-induced
damage.
Further
research
is
warranted
explore
additional
pathways
broader
molecular
Язык: Английский
Phytocannabinoids as Chemotherapy Adjuncts – a User Guide
Опубликована: Авг. 21, 2024
Simple
Summary
(148
words)
There
is
increasing
evidence,
that
cannabinoids
may
play
an
important
and
dual
role
in
tumour
therapy.
On
one
hand,
they
are
cytotoxic
against
cancer
cells
with
a
low
toxicity
normal,
healthy
tissues,
on
the
other,
reduce
typical
side
effects
of
chemotherapy.
Publications
main
motive
for
use
cannabis
(cannabinoids)
by
patients
show
patients'
expectations
exceed
scientific
facts.
This
article
briefly
describes
results
combinations
standard
antineoplastic
drugs
tumours
Although
observations
still
limited
to
animal
studies
very
few
experiences
patients,
preliminary
data
suggest
adjuvant
improve
survival
glioblastoma
perhaps
other
tumours.
Benefits
have
also
been
reported
about
chemotherapy-induced
nausea
vomiting,
peripheral
neuropathic
pain,
loss
appetite
anxiety.
promising,
much
more
research
necessary.
Abstract
(192
Cancer,
leading
causes
death
worldwide,
rise.
The
high
conventional
chemotherapy,
often
applied
as
drug
cocktails,
resistance
development
limits
anti-neoplastic
reduces
quality
life.
With
easier
access,
growing
number
using
alleviation
their
symptoms,
hope
improving
survival.
narrative
summarizes
observed
phytocannabinoids
chemotherapeutic
agents
models
patients.
Preliminary
combined
exert
increased
anti-cancer
actions,
Respective
scarce
concern
small
numbers
glioblastoma,
pancreatic
carcinoma.
Further
benefits
(dronabinol),
pain
anxiety
(cannabidiol).
In
addition,
phytocannabinoids,
particular
cannabidiol,
protection
organs
such
heart,
lung
or
kidney
toxicity.
needed
determine
whether
positive
outweigh
eventual
risks.
Язык: Английский
Phytocannabinoids as Chemotherapy Adjuncts—A Review for Users
Onco,
Год журнала:
2024,
Номер
4(4), С. 287 - 321
Опубликована: Окт. 11, 2024
Cancer,
one
of
the
leading
causes
death
worldwide,
is
on
rise.
The
high
toxicity
conventional
chemotherapy,
often
applied
as
drug
cocktails,
and
development
resistance
limit
use
antineoplastic
drugs
reduce
quality
life.
With
easier
access,
a
growing
number
patients
are
using
cannabis
(cannabinoids)
for
alleviation
their
symptoms,
in
hope
improving
survival.
This
article
summarizes
results
observed
with
combinations
phytocannabinoids
standard
chemotherapeutic
agents
animal
tumour
models
patients.
It
limited
to
approved
phytocannabinoids.
Preliminary
preclinical
data
suggest
that
combined
cannabinoids
exert
enhanced
anti-cancer
effects,
improve
Corresponding
experiences
still
very
only
concern
few
glioblastoma
pancreatic
cancer.
Benefits
containing
have
also
been
reported
chemotherapy-induced
nausea
vomiting,
loss
appetite
(dronabinol),
peripheral
neuropathic
pain
anxiety
(cannabidiol).
In
addition,
phytocannabinoids,
particularly
cannabidiol,
may
play
role
protecting
organs
such
heart,
lungs
or
kidneys
from
chemotherapy-related
toxicity.
Although
promising,
more
research
needed
ensure
whether
benefits
adjuvant
outweigh
potential
risks.
Язык: Английский
Genome-wide CRISPR-based screen identifies E2F transcription factor 1 as a Regulator and therapeutic target of aristolochic acid-induced nephrotoxicity
Environment International,
Год журнала:
2024,
Номер
195, С. 109234 - 109234
Опубликована: Дек. 22, 2024
Aristolochic
Acid
I
(AAI)
is
widely
present
in
traditional
Chinese
medicines
derived
from
the
Aristolochia
genus
and
known
to
cause
significant
damage
renal
tubular
epithelial
cells.
Genome-wide
screening
has
proven
be
a
powerful
tool
identifying
critical
genes
associated
with
toxicity
of
exogenous
substances.
To
identify
undiscovered
key
involved
AAI-induced
toxicity,
genome-wide
CRISPR
library
screen
was
conducted
human
kidney-2
(HK-2)
cell
line.
Among
altered
sgRNAs,
enrichment
those
targeting
E2F
transcription
factor
1
(E2F1)
gene
observed
surviving
HK-2
cells
AAI-treated
group.
Interestingly,
role
E2F1
had
not
been
previously
explored
studies
AAI
nephrotoxicity.
Further
investigations
revealed
that
promotes
apoptosis
by
activating
p53
signaling
pathway
upregulating
pro-apoptotic
genes,
such
as
BAK
BAX.
Additionally,
using
high-throughput
experiment-
reference-guided
database
medicine
(HERB),
cannabidiol
(CBD)
identified
an
inhibitor
suppressing
activity
NF-κB
pathway.
In
vitro
vivo
models
confirmed
CBD
inhibits
upregulation
E2F1,
thereby
p53-mediated
apoptosis.
conclusion,
this
study
highlights
crucial
identifies
novel
therapeutic
candidate
for
mitigating
Язык: Английский
Protective Action of Cannabidiol on Tiamulin Toxicity in Humans—In Vitro Study
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(24), С. 13542 - 13542
Опубликована: Дек. 18, 2024
The
growing
awareness
and
need
to
protect
public
health,
including
food
safety,
require
a
thorough
study
of
the
mechanism
action
veterinary
drugs
in
consumers
reduce
their
negative
impact
on
humans.
Inappropriate
use
animal
husbandry,
such
as
tiamulin,
leads
appearance
residues
edible
tissues.
natural
substances
plant
origin,
extracted
from
hemp
(Cannabis
sativa
L.),
cannabidiol
(CBD),
is
one
solutions
minimize
effects
tiamulin.
This
aimed
determine
effect
CBD
cytotoxicity
tiamulin
cytotoxic
activity
its
mixtures
with
were
tested
after
72
h
exposure
three
human
cell
lines:
SH-SY5Y,
HepG2
HEK-293.
Cytotoxic
concentrations
(IC50)
drug
combination
assessed
using
five
biochemical
endpoints:
mitochondrial
lysosomal
activity,
proliferation,
membrane
integrity
DNA
synthesis.
Oxidative
stress,
death
cellular
morphology
also
assessed.
nature
interaction
between
was
index.
long-term
inhibited
(SH-SY5SY)
(HepG2)
synthesis
(HEK-293).
IC50
values
for
ranged
2.1
>200
µg/mL
(SH-SY5SY),
13.9
39.5
8.5
76.9
drug/CBD
higher.
Reduced
levels
oxidative
apoptosis
changes
demonstrated
mixtures.
Interactions
showed
concentration-dependent
culture,
ranging
antagonistic
(low
concentrations)
synergistic
at
high
concentrations.
increased
risk
health
associated
presence
products
protective
underline
importance
these
studies
toxicology
further
investigation.
Язык: Английский
Impact of Long-Term Cannabidiol (CBD) Treatment on Mouse Kidney Transcriptome
Genes,
Год журнала:
2024,
Номер
15(12), С. 1640 - 1640
Опубликована: Дек. 21, 2024
Background:
Cannabidiol,
which
is
one
of
the
main
cannabinoids
present
in
Cannabis
sativa
plants,
has
been
shown
to
have
therapeutic
properties,
including
anti-inflammatory
and
antioxidant
effects
that
may
be
useful
for
treatment
various
kidney
conditions.
Objectives:
This
article
investigates
effect
long-term
cannabidiol
(CBD)
on
changes
renal
transcriptome
a
mouse
model.
The
hypothesis
was
systematic
CBD
would
affect
gene
expression
associated
with
those
processes
kidney.
Methods:
study
conducted
male
C57BL/6J
mice.
Mice
experimental
groups
received
daily
intraperitoneal
injections
at
doses
10
mg/kg
or
20
body
weight
(b.w.)
28
days.
After
experiment,
tissues
were
collected,
RNA
isolated,
RNA-Seq
sequencing
performed.
Results:
results
show
CBD’s
expression,
regulation
genes
related
circadian
rhythm
(e.g.,
Ciart,
Nr1d1,
Nr1d2,
Per2,
Per3),
glucocorticoid
receptor
function
Cyp1b1,
Ddit4,
Foxo3,
Gjb2,
Pck1),
lipid
metabolism
Cyp2d22,
Cyp2d9,
Decr2
Hacl1,
Sphk1),
inflammatory
response
Cxcr4
Ccl28).
Conclusions:
obtained
suggest
beneficial
purposes
treating
disease,
its
should
further
analyzed
clinical
trials.
Язык: Английский