Recent progresses of derivatization approaches in the targeted lipidomics analysis by mass spectrometry DOI
Xian‐En Zhao, Shuyun Zhu, Huwei Liu

и другие.

Journal of Separation Science, Год журнала: 2020, Номер 43(9-10), С. 1838 - 1846

Опубликована: Фев. 18, 2020

Abstract Lipidomics plays an essential role in the development of improved understanding lipids metabolism and identification new biomarkers or therapeutic targets related diseases. The strong analytical power mass spectrometry its rapid developments respect instruments techniques have significantly accelerated emerging lipidomics application fields biology, medicine, pharmacy. strategy chemical derivatization can remarkably improve shortcomings spectrometric technologies shotgun liquid chromatography spectrometry, past decade many studies been reported for fatty acids, glycerophospholipids, sphingomyelins, monoglycerides, diacylglycerols, long‐chain bases, steroids, so on. Therefore, this review will focus on approaches about research progresses shotgun‐based spectrometry–based targeted (from 2005 to July 2019, most reports emerged 5 years), put forward problems prospects field. It is expected be helpful design synthesis reagents, especially outstanding stable isotope labeling strategies matched analysis methods.

Язык: Английский

Brain lipidomics: From functional landscape to clinical significance DOI Creative Commons
Jong Hyuk Yoon, Youngsuk Seo, Yeon Suk Jo

и другие.

Science Advances, Год журнала: 2022, Номер 8(37)

Опубликована: Сен. 16, 2022

Lipids are crucial components of cellular function owing to their role in membrane formation, intercellular signaling, energy storage, and homeostasis maintenance. In the brain, lipid dysregulations have been associated with etiology progression neurodegeneration other neurological pathologies. Hence, brain lipids emerging as important potential targets for early diagnosis prognosis diseases. This review aims highlight significance usefulness lipidomics diagnosing treating We explored alterations diseases, paying attention organ-specific characteristics functions lipids. As recent advances would impossible without analytical techniques, we provide up-to-date information on mass spectrometric approaches integrative analysis omic approaches. Last, present applications combined artificial intelligence techniques interdisciplinary collaborative research diseases clinical heterogeneities.

Язык: Английский

Процитировано

149

Targeting fatty acid metabolism in glioblastoma DOI Creative Commons
Jason Miska, Navdeep S. Chandel

Journal of Clinical Investigation, Год журнала: 2023, Номер 133(1)

Опубликована: Янв. 2, 2023

Glioblastoma (GBM) is a primary tumor of the brain defined by its uniform lethality and resistance to conventional therapies. There have been considerable efforts untangle metabolic underpinnings this disease find novel therapeutic avenues for treatment. An emerging focus in field fatty acid (FA) metabolism, which critical numerous diverse biological processes involved GBM pathogenesis. These can be classified into four broad fates: anabolism, catabolism, regulation ferroptosis, generation signaling molecules. Each fate provides unique perspective we inspect biology gives us road map understanding complicated field. This Review discusses basic, translational, clinical insights each these fates provide contemporary FA GBM. It clear, based on literature, that there are far more questions than answers metabolism GBM, substantial should made complex intractable disease.

Язык: Английский

Процитировано

61

Recent Analytical Methodologies in Lipid Analysis DOI Open Access
Ivana Gerhardtová, Timotej Jankech, Petra Majerová

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(4), С. 2249 - 2249

Опубликована: Фев. 13, 2024

Lipids represent a large group of biomolecules that are responsible for various functions in organisms. Diseases such as diabetes, chronic inflammation, neurological disorders, or neurodegenerative and cardiovascular diseases can be caused by lipid imbalance. Due to the different stereochemical properties composition fatty acyl groups molecules most classes, quantification lipids development lipidomic analytical techniques problematic. Identification species from complex matrices is difficult, therefore individual steps, which include extraction, separation, detection lipids, must chosen properly. This review critically documents recent strategies analysis sample pretreatment instrumental data interpretation published last five years (2019 2023). The advantages disadvantages extraction methods covered. step comprises identification quantification. Mass spectrometry (MS) used technique analysis, performed direct infusion MS approach combination with suitable separation liquid chromatography gas chromatography. Special attention also given correct evaluation obtained analyses. Only accurate, precise, robust reliable able bring useful information, may contribute clarification some at molecular level, putative biomarkers and/or therapeutic targets.

Язык: Английский

Процитировано

18

Lipidomic approaches to dissect dysregulated lipid metabolism in kidney disease DOI
Judy Baek, Chenchen He, Farsad Afshinnia

и другие.

Nature Reviews Nephrology, Год журнала: 2021, Номер 18(1), С. 38 - 55

Опубликована: Окт. 6, 2021

Язык: Английский

Процитировано

103

Lipids from Microalgae for Cosmetic Applications DOI Creative Commons
Maria De Luca, Ilaria Pappalardo,

Antonina Rita Limongi

и другие.

Cosmetics, Год журнала: 2021, Номер 8(2), С. 52 - 52

Опубликована: Июнь 17, 2021

In recent years, there has been considerable interest in using microalgal lipids the food, chemical, pharmaceutical, and cosmetic industries. Several species can accumulate appreciable lipid quantities therefore are characterized as oleaginous. formulations, their derivatives one of main ingredients. Different classes great moisturizing, emollient, softening agents, work surfactants emulsifiers, give consistence to products, color fragrance carriers, act preservatives maintain products integrity, be part molecules delivery system. past, chemicals have widely used but today’s market customers’ demands oriented towards natural products. Microalgae an extraordinary source other many bioactive molecules. Scientists’ attention microalgae cultivation for industrial application is increasing. For high costs associated, commercialization still not very widespread. The possibility use biomass various purposes could make more economically competitive.

Язык: Английский

Процитировано

89

A DMS Shotgun Lipidomics Workflow Application to Facilitate High-Throughput, Comprehensive Lipidomics DOI
Baolong Su, Lisa Bettcher,

Wei-Yuan Hsieh

и другие.

Journal of the American Society for Mass Spectrometry, Год журнала: 2021, Номер 32(11), С. 2655 - 2663

Опубликована: Окт. 12, 2021

Differential mobility spectrometry (DMS) is highly useful for shotgun lipidomic analysis because it overcomes difficulties in measuring isobaric species within a complex lipid sample and allows acyl tail characterization of phospholipid species. Despite these advantages, the resulting workflow presents technical challenges, including need to tune DMS before every batch update compensative voltages settings method. The Sciex Lipidyzer platform uses 5500 QTRAP with (SelexION), an LC system configured direction infusion experiments, extensive set standards designed quantitative lipidomics, software package (Lipidyzer Workflow Manager) that facilitates rapidly analyzes data. Although remains very DMS-based no longer updated current versions Analyst Windows. Furthermore, fixed single cannot take advantage new lipidomics or analyze additional To address this multitude issues, we developed Shotgun Lipidomics Assistant (SLA), Python-based application workflows. SLA provides user flexibility adding subtracting standard MRMs. It can report results from raw data minutes, comparable software. We show expanded measures over 1450 across 17 (sub)classes. Lastly, demonstrate performs isotope correction, feature was absent original

Язык: Английский

Процитировано

89

Linking Gut Microbiome and Lipid Metabolism: Moving beyond Associations DOI Creative Commons
Santosh Lamichhane, Partho Sen, Marina Amaral Alves

и другие.

Metabolites, Год журнала: 2021, Номер 11(1), С. 55 - 55

Опубликована: Янв. 15, 2021

Various studies aiming to elucidate the role of gut microbiome-metabolome co-axis in health and disease have primarily focused on water-soluble polar metabolites, whilst non-polar microbial lipids received less attention. The concept microbiota-dependent lipid biotransformation is over a century old. However, only recently, several shown how alter intestinal circulating concentrations host, thus impacting human homeostasis. There emerging evidence that communities play particularly significant regulation host cholesterol sphingolipid Here, we review discuss recent research focusing microbe-host-lipid co-metabolism. We also interplay microbiota molecular entering systemic circulation, its disease.

Язык: Английский

Процитировано

78

Membrane lipids and transporter function DOI Creative Commons
Bruno Stieger,

Julia Steiger,

Kaspar P. Locher

и другие.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Год журнала: 2021, Номер 1867(5), С. 166079 - 166079

Опубликована: Янв. 19, 2021

Transport proteins are essential for cells in allowing the exchange of substances between and their environment across lipid bilayer forming a tight barrier. Membrane lipids modulate function transmembrane such as transporters two ways: Lipids tightly specifically bound to transport addition they from bulk proteins. This overview summarizes currently available information at ultrastructural level on impact altered membrane composition. Human diseases leading homeostasis will lead composition, which turn affect transporter

Язык: Английский

Процитировано

59

Integrating lipidomics and genomics: emerging tools to understand cardiovascular diseases DOI Creative Commons
Rubina Tabassum, Samuli Ripatti

Cellular and Molecular Life Sciences, Год журнала: 2021, Номер 78(6), С. 2565 - 2584

Опубликована: Янв. 15, 2021

Cardiovascular diseases (CVDs) are the leading cause of mortality and morbidity worldwide to 31% all global deaths. Early prediction prevention could greatly reduce enormous socio-economic burden posed by CVDs. Plasma lipids have been at center stage strategies for CVDs that mostly relied on traditional (total cholesterol, total triglycerides, HDL-C LDL-C). The tremendous advancement in field lipidomics last two decades has facilitated research efforts unravel metabolic dysregulation their genetic determinants, enabling understanding pathophysiological mechanisms identification predictive biomarkers, beyond lipids. This review presents an overview application epidemiological studies contributions current field. We findings these discuss examples demonstrates potential revealing new biology not captured lipoprotein measurements. promising from raised opportunities fields personalized medicine further discusses prospects integrating emerging genomics tools with high-dimensional lipidome move forward statistical associations towards biological understanding, therapeutic target development risk prediction. believe holds a great but advancements computational needed handle correlated lipidome.

Язык: Английский

Процитировано

58

A new update of MALDI-TOF mass spectrometry in lipid research DOI

Kathrin M. Engel,

Patricia Prabutzki, Jenny Leopold

и другие.

Progress in Lipid Research, Год журнала: 2022, Номер 86, С. 101145 - 101145

Опубликована: Янв. 5, 2022

Язык: Английский

Процитировано

58