Molecular Omics, Journal Year: 2022, Volume and Issue: 18(5), P. 357 - 386
Published: Jan. 1, 2022
A compressive overview of LC-MS-based marine lipidomics: methodological workflow, lipid characterization, quantification and the most recent findings in phyla.
Language: Английский
Science Advances, Journal Year: 2022, Volume and Issue: 8(37)
Published: Sept. 16, 2022
Lipids are crucial components of cellular function owing to their role in membrane formation, intercellular signaling, energy storage, and homeostasis maintenance. In the brain, lipid dysregulations have been associated with etiology progression neurodegeneration other neurological pathologies. Hence, brain lipids emerging as important potential targets for early diagnosis prognosis diseases. This review aims highlight significance usefulness lipidomics diagnosing treating We explored alterations diseases, paying attention organ-specific characteristics functions lipids. As recent advances would impossible without analytical techniques, we provide up-to-date information on mass spectrometric approaches integrative analysis omic approaches. Last, present applications combined artificial intelligence techniques interdisciplinary collaborative research diseases clinical heterogeneities.
Language: Английский
Citations
143
Journal of Clinical Investigation, Journal Year: 2023, Volume and Issue: 133(1)
Published: Jan. 2, 2023
Glioblastoma (GBM) is a primary tumor of the brain defined by its uniform lethality and resistance to conventional therapies. There have been considerable efforts untangle metabolic underpinnings this disease find novel therapeutic avenues for treatment. An emerging focus in field fatty acid (FA) metabolism, which critical numerous diverse biological processes involved GBM pathogenesis. These can be classified into four broad fates: anabolism, catabolism, regulation ferroptosis, generation signaling molecules. Each fate provides unique perspective we inspect biology gives us road map understanding complicated field. This Review discusses basic, translational, clinical insights each these fates provide contemporary FA GBM. It clear, based on literature, that there are far more questions than answers metabolism GBM, substantial should made complex intractable disease.
Language: Английский
Citations
59
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(4), P. 2249 - 2249
Published: Feb. 13, 2024
Lipids represent a large group of biomolecules that are responsible for various functions in organisms. Diseases such as diabetes, chronic inflammation, neurological disorders, or neurodegenerative and cardiovascular diseases can be caused by lipid imbalance. Due to the different stereochemical properties composition fatty acyl groups molecules most classes, quantification lipids development lipidomic analytical techniques problematic. Identification species from complex matrices is difficult, therefore individual steps, which include extraction, separation, detection lipids, must chosen properly. This review critically documents recent strategies analysis sample pretreatment instrumental data interpretation published last five years (2019 2023). The advantages disadvantages extraction methods covered. step comprises identification quantification. Mass spectrometry (MS) used technique analysis, performed direct infusion MS approach combination with suitable separation liquid chromatography gas chromatography. Special attention also given correct evaluation obtained analyses. Only accurate, precise, robust reliable able bring useful information, may contribute clarification some at molecular level, putative biomarkers and/or therapeutic targets.
Language: Английский
Citations
18
Nature Reviews Nephrology, Journal Year: 2021, Volume and Issue: 18(1), P. 38 - 55
Published: Oct. 6, 2021
Language: Английский
Citations
100
Cosmetics, Journal Year: 2021, Volume and Issue: 8(2), P. 52 - 52
Published: June 17, 2021
In recent years, there has been considerable interest in using microalgal lipids the food, chemical, pharmaceutical, and cosmetic industries. Several species can accumulate appreciable lipid quantities therefore are characterized as oleaginous. formulations, their derivatives one of main ingredients. Different classes great moisturizing, emollient, softening agents, work surfactants emulsifiers, give consistence to products, color fragrance carriers, act preservatives maintain products integrity, be part molecules delivery system. past, chemicals have widely used but today’s market customers’ demands oriented towards natural products. Microalgae an extraordinary source other many bioactive molecules. Scientists’ attention microalgae cultivation for industrial application is increasing. For high costs associated, commercialization still not very widespread. The possibility use biomass various purposes could make more economically competitive.
Language: Английский
Citations
88
Journal of the American Society for Mass Spectrometry, Journal Year: 2021, Volume and Issue: 32(11), P. 2655 - 2663
Published: Oct. 12, 2021
Differential mobility spectrometry (DMS) is highly useful for shotgun lipidomic analysis because it overcomes difficulties in measuring isobaric species within a complex lipid sample and allows acyl tail characterization of phospholipid species. Despite these advantages, the resulting workflow presents technical challenges, including need to tune DMS before every batch update compensative voltages settings method. The Sciex Lipidyzer platform uses 5500 QTRAP with (SelexION), an LC system configured direction infusion experiments, extensive set standards designed quantitative lipidomics, software package (Lipidyzer Workflow Manager) that facilitates rapidly analyzes data. Although remains very DMS-based no longer updated current versions Analyst Windows. Furthermore, fixed single cannot take advantage new lipidomics or analyze additional To address this multitude issues, we developed Shotgun Lipidomics Assistant (SLA), Python-based application workflows. SLA provides user flexibility adding subtracting standard MRMs. It can report results from raw data minutes, comparable software. We show expanded measures over 1450 across 17 (sub)classes. Lastly, demonstrate performs isotope correction, feature was absent original
Language: Английский
Citations
86
Metabolites, Journal Year: 2021, Volume and Issue: 11(1), P. 55 - 55
Published: Jan. 15, 2021
Various studies aiming to elucidate the role of gut microbiome-metabolome co-axis in health and disease have primarily focused on water-soluble polar metabolites, whilst non-polar microbial lipids received less attention. The concept microbiota-dependent lipid biotransformation is over a century old. However, only recently, several shown how alter intestinal circulating concentrations host, thus impacting human homeostasis. There emerging evidence that communities play particularly significant regulation host cholesterol sphingolipid Here, we review discuss recent research focusing microbe-host-lipid co-metabolism. We also interplay microbiota molecular entering systemic circulation, its disease.
Language: Английский
Citations
77
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2021, Volume and Issue: 1867(5), P. 166079 - 166079
Published: Jan. 19, 2021
Transport proteins are essential for cells in allowing the exchange of substances between and their environment across lipid bilayer forming a tight barrier. Membrane lipids modulate function transmembrane such as transporters two ways: Lipids tightly specifically bound to transport addition they from bulk proteins. This overview summarizes currently available information at ultrastructural level on impact altered membrane composition. Human diseases leading homeostasis will lead composition, which turn affect transporter
Language: Английский
Citations
59
Progress in Lipid Research, Journal Year: 2022, Volume and Issue: 86, P. 101145 - 101145
Published: Jan. 5, 2022
Language: Английский
Citations
58
Journal of Lipid Research, Journal Year: 2022, Volume and Issue: 63(7), P. 100219 - 100219
Published: April 27, 2022
In-depth structural characterization of lipids is an essential component lipidomics. There has been a rapid expansion mass spectrometry methods that are capable resolving lipid isomers at various levels over the past decade. These developments finally make deep-lipidotyping possible, which provides new means to study metabolism and discover biomarkers. In this review, we discuss recent advancements in tandem (MS/MS) for identification complex beyond species (known headgroup information) molecular chain composition) levels. include carbon-carbon double bond (C=C) location sn-position, as well acyl modifications. We also integration isomer-resolving MS/MS with different analysis workflows their applications The results showcase distinct capabilities untangling individual sensitive phenotyping by using relative fractional quantitation isomers.
Language: Английский
Citations
53