Journal of Separation Science,
Год журнала:
2020,
Номер
43(9-10), С. 1838 - 1846
Опубликована: Фев. 18, 2020
Abstract
Lipidomics
plays
an
essential
role
in
the
development
of
improved
understanding
lipids
metabolism
and
identification
new
biomarkers
or
therapeutic
targets
related
diseases.
The
strong
analytical
power
mass
spectrometry
its
rapid
developments
respect
instruments
techniques
have
significantly
accelerated
emerging
lipidomics
application
fields
biology,
medicine,
pharmacy.
strategy
chemical
derivatization
can
remarkably
improve
shortcomings
spectrometric
technologies
shotgun
liquid
chromatography
spectrometry,
past
decade
many
studies
been
reported
for
fatty
acids,
glycerophospholipids,
sphingomyelins,
monoglycerides,
diacylglycerols,
long‐chain
bases,
steroids,
so
on.
Therefore,
this
review
will
focus
on
approaches
about
research
progresses
shotgun‐based
spectrometry–based
targeted
(from
2005
to
July
2019,
most
reports
emerged
5
years),
put
forward
problems
prospects
field.
It
is
expected
be
helpful
design
synthesis
reagents,
especially
outstanding
stable
isotope
labeling
strategies
matched
analysis
methods.
Talanta,
Год журнала:
2025,
Номер
290, С. 127811 - 127811
Опубликована: Фев. 23, 2025
Endocannabinoids
(eCBs)
are
endogenous
lipid
messengers
that
primarily
bind
cannabinoid
receptors
CB1/CB2
and
together
with
the
enzymes
regulate
their
biosynthesis
degradation
define
endocannabinoid
system.
The
eCB
signaling
system
plays
a
key
role
in
central
nervous
system,
results
often
altered
neurological
disorders.
analysis
of
eCBs
is
challenging
due
to
low
concentration
biospecimens,
this
exacerbated
Mass
Spectrometry
Imaging
(MSI)
where
sensitivity
tissue
dependent
ion
suppression
obscure
spatial
visualization.
In
work
we
address
limitation
by
application
laser-induced
post-ionization
(MALDI-2)
approach.
Herein
demonstrate
MALDI-2
boosts
detection
2-arachidonylglycerol
(2-AG)
N-acylethanolamines
(AEA,
PEA,
OEA)
respect
MALDI,
can
be
visualized
brain
at
physiological
only
MALDI-2-MSI.
Root-mean-square
(RMS),
Total
count
(TIC)
internal
standards
(I.S.)
normalization
were
evaluated,
I.S.
providing
improved
pixel
variation
more
uniform
distribution
for
2-AG
PEA
specific
regions.
Furthermore,
high
resolution
up
5
μm
size
was
resulting
all
confirming
potential
even
reducing
ablated
amount.
As
proof
concept,
method
applied
map
mouse
model
mild
traumatic
injury,
APP-SWE
mice,
highlighting
differences
modulation
Cortex,
Hippocampus
Hypothalamus,
suggesting
ability
reveal
valuable
biological
insights
neuropharmacology.
Journal of Extracellular Vesicles,
Год журнала:
2025,
Номер
14(3)
Опубликована: Март 1, 2025
ABSTRACT
Quantitative
lipidomic
analysis
performed
by
mass
spectrometry
is
required
for
determination
of
the
lipid
content
extracellular
vesicles
(EVs).
Such
methods
can
provide
information
about
total
amount
lipids,
species
composition,
purity
EV
samples
as
well
cellular
origin
EVs.
There
are,
however,
many
pitfalls
when
performing
analyses.
Thus,
any
non‐specialist
should
collaborate
with
experts
in
lipidomics.
In
addition
to
good
review
articles
giving
advice
analyses,
we
recommend
and
guidelines
published
Lipidomic
Standard
Initiative,
an
interest
group
affiliated
International
Lipidomics
Society.
Analytical Chemistry,
Год журнала:
2021,
Номер
93(16), С. 6311 - 6322
Опубликована: Апрель 15, 2021
Lipids
and
metabolites
are
of
interest
in
many
clinical
research
settings
because
it
is
the
metabolome
that
increasingly
recognized
as
a
more
dynamic
sensitive
molecular
measure
phenotype.
The
enormous
diversity
lipid
structures
importance
biological
structure-function
relationships
wide
variety
applications
makes
accurate
identification
challenging
yet
crucial
area
community.
Indeed,
subtle
differences
chemical
lipids
can
have
important
implications
cellular
metabolism
disease
pathologies.
speed,
sensitivity,
specificity
afforded
by
modern
mass
spectrometry
has
led
to
its
widespread
adoption
field
lipidomics
on
different
instrument
platforms
experimental
workflows.
However,
unambiguous
complete
structural
remains
challenging.
Increasingly
sophisticated
tandem
(MS/MS)
approaches
now
being
developed
seamlessly
integrated
into
workflows
meet
this
challenge.
These
generally
either
(i)
alter
type
ion
interrogated
or
(ii)
dissociation
method
order
improve
information
obtained
from
MS/MS
experiment.
In
Perspective,
we
highlight
recent
advances
both
alteration
methods
for
spectrometry.
This
discussion
aimed
engage
investigators
involved
fundamental
chemistry
technology
developments
well
practitioners
applications.
rapid
rate
development
years
accelerated
strengthened
ties
between
these
two
communities.
We
identify
common
characteristics
practical
figures
merit
emerging
discuss
ways
may
catalyze
future
directions
elucidation
research.
Journal of Separation Science,
Год журнала:
2020,
Номер
43(9-10), С. 1838 - 1846
Опубликована: Фев. 18, 2020
Abstract
Lipidomics
plays
an
essential
role
in
the
development
of
improved
understanding
lipids
metabolism
and
identification
new
biomarkers
or
therapeutic
targets
related
diseases.
The
strong
analytical
power
mass
spectrometry
its
rapid
developments
respect
instruments
techniques
have
significantly
accelerated
emerging
lipidomics
application
fields
biology,
medicine,
pharmacy.
strategy
chemical
derivatization
can
remarkably
improve
shortcomings
spectrometric
technologies
shotgun
liquid
chromatography
spectrometry,
past
decade
many
studies
been
reported
for
fatty
acids,
glycerophospholipids,
sphingomyelins,
monoglycerides,
diacylglycerols,
long‐chain
bases,
steroids,
so
on.
Therefore,
this
review
will
focus
on
approaches
about
research
progresses
shotgun‐based
spectrometry–based
targeted
(from
2005
to
July
2019,
most
reports
emerged
5
years),
put
forward
problems
prospects
field.
It
is
expected
be
helpful
design
synthesis
reagents,
especially
outstanding
stable
isotope
labeling
strategies
matched
analysis
methods.
