Perspective on Emerging Mass Spectrometry Technologies for Comprehensive Lipid Structural Elucidation

Analytical Chemistry, Journal Year: 2021, Volume and Issue: 93(16), P. 6311 - 6322

Published: April 15, 2021

Lipids and metabolites are of interest in many clinical research settings because it is the metabolome that increasingly recognized as a more dynamic sensitive molecular measure phenotype. The enormous diversity lipid structures importance biological structure-function relationships wide variety applications makes accurate identification challenging yet crucial area community. Indeed, subtle differences chemical lipids can have important implications cellular metabolism disease pathologies. speed, sensitivity, specificity afforded by modern mass spectrometry has led to its widespread adoption field lipidomics on different instrument platforms experimental workflows. However, unambiguous complete structural remains challenging. Increasingly sophisticated tandem (MS/MS) approaches now being developed seamlessly integrated into workflows meet this challenge. These generally either (i) alter type ion interrogated or (ii) dissociation method order improve information obtained from MS/MS experiment. In Perspective, we highlight recent advances both alteration methods for spectrometry. This discussion aimed engage investigators involved fundamental chemistry technology developments well practitioners applications. rapid rate development years accelerated strengthened ties between these two communities. We identify common characteristics practical figures merit emerging discuss ways may catalyze future directions elucidation research.

Language: Английский

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Recent progresses of derivatization approaches in the targeted lipidomics analysis by mass spectrometry

Journal of Separation Science, Journal Year: 2020, Volume and Issue: 43(9-10), P. 1838 - 1846

Published: Feb. 18, 2020

Abstract Lipidomics plays an essential role in the development of improved understanding lipids metabolism and identification new biomarkers or therapeutic targets related diseases. The strong analytical power mass spectrometry its rapid developments respect instruments techniques have significantly accelerated emerging lipidomics application fields biology, medicine, pharmacy. strategy chemical derivatization can remarkably improve shortcomings spectrometric technologies shotgun liquid chromatography spectrometry, past decade many studies been reported for fatty acids, glycerophospholipids, sphingomyelins, monoglycerides, diacylglycerols, long‐chain bases, steroids, so on. Therefore, this review will focus on approaches about research progresses shotgun‐based spectrometry–based targeted (from 2005 to July 2019, most reports emerged 5 years), put forward problems prospects field. It is expected be helpful design synthesis reagents, especially outstanding stable isotope labeling strategies matched analysis methods.

Language: Английский

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Defining Blood Plasma and Serum Metabolome by GC-MS

Metabolites, Journal Year: 2021, Volume and Issue: 12(1), P. 15 - 15

Published: Dec. 24, 2021

Metabolomics uses advanced analytical chemistry methods to analyze metabolites in biological samples. The most intensively studied samples are blood and its liquid components: plasma serum. Armed with equipment progressive software solutions, the scientific community has shown that small molecules’ roles living systems not limited traditional “building blocks” or “just fuel” for cellular energy. As a result, conclusions based on studying metabolome finding practical reflection molecular medicine better understanding of fundamental biochemical processes systems. This review is detailed protocol metabolomic analysis. However, it should support reader information about achievements whole process metabolic exploration human serum using mass spectrometry combined gas chromatography.

Language: Английский

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Mitochondrial Fatty Acid β-Oxidation Disorders: From Disease to Lipidomic Studies—A Critical Review

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(22), P. 13933 - 13933

Published: Nov. 11, 2022

Fatty acid oxidation disorders (FAODs) are inborn errors of metabolism (IEMs) caused by defects in the fatty (FA) mitochondrial β-oxidation. The most common FAODs characterized accumulation medium-chain FAs and long-chain (3-hydroxy) (and their carnitine derivatives), respectively. These deregulations associated with lipotoxicity which affects several organs potentially leads to life-threatening complications comorbidities. Changes lipidome have been diseases, including some IEMs. In FAODs, alteration acylcarnitines (CARs) FA profiles reported patients animal models, but changes polar neutral lipid profile still scarcely studied. this review, we present main findings on CAR FAOD pathogenesis, correlation oxidative damage, consequent disturbance homeostasis. Moreover, alterations classes species identified so far possible role discussed. We highlight need mass-spectrometry-based lipidomic studies understand (epi)lipidome remodelling thus allowing elucidate pathophysiology identification biomarkers for disease prognosis an evaluation therapeutic efficacy.

Language: Английский

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Applications of lipidomics in marine organisms: progress, challenges and future perspectives

Molecular Omics, Journal Year: 2022, Volume and Issue: 18(5), P. 357 - 386

Published: Jan. 1, 2022

A compressive overview of LC-MS-based marine lipidomics: methodological workflow, lipid characterization, quantification and the most recent findings in phyla.

Language: Английский

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