The
global
rise
of
antibiotic
resistance
calls
for
new
drugs
against
bacterial
pathogens.
A
common
approach
is
to
search
natural
compounds
deployed
by
microbes
inhibit
competitors.
Here,
we
show
that
the
iron-chelating
pyoverdines,
siderophores
produced
environmental
Pseudomonas
spp.,
have
strong
antibacterial
properties
inducing
iron
starvation
and
growth
arrest
in
screen
320
isolates
used
12
human
pathogens
uncovered
several
pyoverdines
with
particularly
high
distinct
chemical
characteristics.
most
potent
pyoverdine
effectively
reduced
Acinetobacter
baumannii
,
Klebsiella
pneumoniae,
Staphylococcus
aureus
a
concentration-
iron-dependent
manner.
Pyoverdine
increased
survival
infected
Galleria
mellonella
host
larvae
showed
low
toxicity
host,
mammalian
cell
lines,
erythrocytes.
Furthermore,
experimental
evolution
combined
whole-genome
sequencing
revealed
limited
compared
an
antibiotic.
Thus,
from
strains
potential
become
class
sustainable
antibacterials
specific
The Science of The Total Environment,
Год журнала:
2024,
Номер
932, С. 173044 - 173044
Опубликована: Май 8, 2024
Siderophores
are
small
molecules
of
organic
nature,
released
by
bacteria
to
chelate
iron
from
the
surrounding
environment
and
subsequently
incorporate
it
into
cytoplasm.
In
addition
iron,
these
secondary
metabolites
can
complex
with
a
wide
variety
metals,
which
is
why
they
commonly
studied
in
environment.
Heavy
metals
be
very
toxic
when
present
large
amounts
on
planet,
affecting
public
health
all
living
organisms.
The
pollution
caused
increasing,
therefore
urgent
find
practical,
sustainable,
economical
solutions
for
remediation.
One
strategies
siderophore-assisted
bioremediation,
an
innovative
advantageous
alternative
various
environmental
applications.
This
research
highlights
uses
siderophores
metallophores
environment,
underscoring
their
significance
ecosystems.
study
delves
utilization
both
marine
terrestrial
settings
(e.g.
biocontrol
pathogens,
plant
growth
promotion),
such
as
promotion,
providing
context
different
instances
outlined
existing
literature
highlighting
relevance
each
field.
structures
types
focusing
singular
characteristics
application
methodologies
used.
Focusing
recent
developments
over
last
two
decades,
opportunities
challenges
associated
applications
were
mapped
arm
researchers
fight
against
pollution.
Abstract
Microorganisms
produce
siderophores,
which
are
low-molecular-weight
iron
chelators
when
availability
is
limited.
The
present
analyzed
the
role
of
LNPF1
as
multifarious
PGPR
for
improving
growth
parameters
and
nutrient
content
in
peanut
soil
nutrients.
Such
strains
can
be
used
effective
bioinoculants
farming.
In
this
work,
rhizosphere
bacteria
from
Zea
mays
Arachis
hypogaea
plants
Salem
area
Tamil
Nadu,
India,
were
isolated
tested
biochemical
attributes
characteristics
that
stimulate
plant
growth,
such
production
hydrogen
cyanide,
ammonia
(6
µg/mL),
indole
acetic
acid
(76.35
solubilizing
phosphate
(520
µg/mL).
16S
rRNA
gene
sequences
identified
isolate
Pseudomonas
fluorescens
with
a
similarity
percentage
99%
sp.
Isolate
was
evaluated
siderophore.
Siderophore-rich
supernatant
using
Sep
Pack
C18
column
Amberlite-400
Resin
Column
(
λ
max
264)
produced
298
mg/L
50
siderophore,
respectively.
characterization
purified
siderophore
by
TLC,
HPLC,
FTIR,
2D-NMR
analysis
compound
desferrioxamine,
hydroxamate
A
pot
culture
experiment
determined
potential
to
improve
oil
photosynthetic
pigments
L.
content.
Inoculation
A.
hypogea
seeds
improved
leaf
length
(60%),
shoot
(22%),
root
(54.68%),
fresh
weight
(47.28%),
dry
(37%),
number
nuts
(66.66)
compared
control
(untreated
seeds).
This
inoculation
also
(43.42),
short
(38.38%),
seed
(46.72%),
(31.68%),
carotenoid
(64.40%),
total
chlorophyll
(98.%)
Bacterized
showed
substantial
increase
nodulation
(61.65%)
individual
nodules
(95.97)
vis-à-vis
control.
results
study
indicated
P.
might
utilized
bioinoculant
content,
Arachishypogaea
L.,
enrich
BioMetals,
Год журнала:
2022,
Номер
36(4), С. 777 - 797
Опубликована: Дек. 12, 2022
Iron
is
important
for
bacterial
growth
and
survival,
as
it
a
common
co-factor
in
essential
enzymes.
Although
iron
very
abundant
the
earth
crust,
its
bioavailability
low
most
habitats
because
ferric
largely
insoluble
under
aerobic
conditions
at
neutral
pH.
Consequently,
bacteria
have
evolved
plethora
of
mechanisms
to
solubilize
acquire
from
environmental
host
stocks.
In
this
review,
I
focus
on
Pseudomonas
spp.
first
present
main
uptake
taxa,
which
involve
direct
ferrous
via
importers,
production
iron-chelating
siderophores,
exploitation
siderophores
produced
by
other
microbial
species,
use
compounds
plants
animals.
second
part
elaborate
how
these
affect
interactions
between
communities,
their
hosts.
This
live
diverse
communities
certain
iron-uptake
strategies
might
not
only
nutrient,
but
also
gain
relative
advantages
over
competitors
race
iron.
Thus,
an
integrative
understanding
acquisition
eco-evolutionary
dynamics
they
drive
community
level
prove
useful
understand
why
spp.,
particular,
many
general,
such
repertoires.
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(19), С. 11507 - 11507
Опубликована: Сен. 29, 2022
Pyoverdines
(PVDs)
are
a
class
of
siderophores
produced
mostly
by
members
the
genus
Pseudomonas.
Their
primary
function
is
to
accumulate,
mobilize,
and
transport
iron
necessary
for
cell
metabolism.
Moreover,
PVDs
also
play
crucial
role
in
microbes'
survival
mediating
biofilm
formation
virulence.
In
this
review,
we
reorganize
information
recent
years
regarding
biosynthesis
pathogenic
mechanisms,
since
extremely
valuable
compounds.
Additionally,
summarize
therapeutic
applications
deriving
from
PVDs'
use
focus
on
their
as
target
themselves.
We
assess
current
biotechnological
different
sectors
evaluate
state-of-the-art
technology
relating
synthetic
biology
tools
pathway
engineering.
Finally,
review
most
methods
techniques
capable
identifying
such
molecules
complex
matrices
drug-discovery
purposes.
The
global
rise
of
antibiotic
resistance
calls
for
new
drugs
against
bacterial
pathogens.
A
common
approach
is
to
search
natural
compounds
deployed
by
microbes
inhibit
competitors.
Here,
we
show
that
the
iron-chelating
pyoverdines,
siderophores
produced
environmental
Pseudomonas
spp.,
have
strong
antibacterial
properties
inducing
iron
starvation
and
growth
arrest
in
screen
320
isolates
used
12
human
pathogens
uncovered
several
pyoverdines
with
particularly
high
distinct
chemical
characteristics.
most
potent
pyoverdine
effectively
reduced
Acinetobacter
baumannii
,
Klebsiella
pneumoniae,
Staphylococcus
aureus
a
concentration-
iron-dependent
manner.
Pyoverdine
increased
survival
infected
Galleria
mellonella
host
larvae
showed
low
toxicity
host,
mammalian
cell
lines,
erythrocytes.
Furthermore,
experimental
evolution
combined
whole-genome
sequencing
revealed
limited
compared
an
antibiotic.
Thus,
from
strains
potential
become
class
sustainable
antibacterials
specific
Microbial
secondary
metabolites
are
a
rich
source
for
pharmaceutical
discoveries
and
play
crucial
ecological
functions.
While
tools
exist
to
identify
metabolite
clusters
in
genomes,
precise
sequence-to-function
mapping
remains
challenging
because
neither
function
nor
substrate
specificity
of
biosynthesis
enzymes
can
accurately
be
predicted.
Here,
we
developed
knowledge-guided
bioinformatic
pipeline
solve
these
issues.
We
analyzed
1928
genomes
Pseudomonas
bacteria
focused
on
iron-scavenging
pyoverdines
as
model
metabolites.
Our
predicted
188
chemically
different
with
nearly
100%
structural
accuracy
the
presence
94
distinct
receptor
groups
required
uptake
iron-loaded
pyoverdines.
unveils
an
enormous
yet
overlooked
diversity
siderophores
(151
new
structures)
receptors
(91
groups).
approach,
combining
feature
sequence
phylogenetic
approaches,
is
extendable
other
microbial
genera,
thus
emerges
powerful
tool
reconstruct
bacterial
metabolism
pathways
based
data.
ACS Omega,
Год журнала:
2024,
Номер
9(25), С. 26863 - 26877
Опубликована: Июнь 11, 2024
Siderophores
are
well-recognized
low-molecular-weight
compounds
produced
by
numerous
microorganisms
to
acquire
iron
from
the
surrounding
environments.
These
secondary
metabolites
can
form
complexes
with
other
metals
besides
iron,
forming
soluble
metallophores;
because
of
that,
they
widely
investigated
in
either
medicinal
or
environmental
field.
One
bottlenecks
siderophore
research
is
related
identification
new
siderophores
microbial
sources.
Herein
we
have
compiled
a
comprehensive
range
standard
and
updated
methodologies
that
been
developed
over
past
few
years
provide
toolbox
this
area
current
researchers.
