Antimicrobial activity of iron-depriving pyoverdines against human opportunistic pathogens DOI Creative Commons
Vera Vollenweider, Karoline Rehm, Clara Chepkirui

и другие.

eLife, Год журнала: 2024, Номер 13

Опубликована: Дек. 18, 2024

The global rise of antibiotic resistance calls for new drugs against bacterial pathogens. A common approach is to search natural compounds deployed by microbes inhibit competitors. Here, we show that the iron-chelating pyoverdines, siderophores produced environmental Pseudomonas spp., have strong antibacterial properties inducing iron starvation and growth arrest in screen 320 isolates used 12 human pathogens uncovered several pyoverdines with particularly high distinct chemical characteristics. most potent pyoverdine effectively reduced Acinetobacter baumannii , Klebsiella pneumoniae, Staphylococcus aureus a concentration- iron-dependent manner. Pyoverdine increased survival infected Galleria mellonella host larvae showed low toxicity host, mammalian cell lines, erythrocytes. Furthermore, experimental evolution combined whole-genome sequencing revealed limited compared an antibiotic. Thus, from strains potential become class sustainable antibacterials specific

Язык: Английский

Siderophores and metallophores: Metal complexation weapons to fight environmental pollution DOI Creative Commons

Ana F.R. Gomes,

Mariana C. Almeida, Emı́lia Sousa

и другие.

The Science of The Total Environment, Год журнала: 2024, Номер 932, С. 173044 - 173044

Опубликована: Май 8, 2024

Siderophores are small molecules of organic nature, released by bacteria to chelate iron from the surrounding environment and subsequently incorporate it into cytoplasm. In addition iron, these secondary metabolites can complex with a wide variety metals, which is why they commonly studied in environment. Heavy metals be very toxic when present large amounts on planet, affecting public health all living organisms. The pollution caused increasing, therefore urgent find practical, sustainable, economical solutions for remediation. One strategies siderophore-assisted bioremediation, an innovative advantageous alternative various environmental applications. This research highlights uses siderophores metallophores environment, underscoring their significance ecosystems. study delves utilization both marine terrestrial settings (e.g. biocontrol pathogens, plant growth promotion), such as promotion, providing context different instances outlined existing literature highlighting relevance each field. structures types focusing singular characteristics application methodologies used. Focusing recent developments over last two decades, opportunities challenges associated applications were mapped arm researchers fight against pollution.

Язык: Английский

Процитировано

22

Perspective on the biotechnological production of bacterial siderophores and their use DOI
Eduardo V. Soares

Applied Microbiology and Biotechnology, Год журнала: 2022, Номер 106(11), С. 3985 - 4004

Опубликована: Июнь 1, 2022

Язык: Английский

Процитировано

57

Purification and Characterization of Desferrioxamine B of Pseudomonas fluorescens and Its Application to Improve Oil Content, Nutrient Uptake, and Plant Growth in Peanuts DOI Creative Commons

S. Nithyapriya,

Lalitha Sundaram, Sakthi Uma Devi Eswaran

и другие.

Microbial Ecology, Год журнала: 2024, Номер 87(1)

Опубликована: Апрель 17, 2024

Abstract Microorganisms produce siderophores, which are low-molecular-weight iron chelators when availability is limited. The present analyzed the role of LNPF1 as multifarious PGPR for improving growth parameters and nutrient content in peanut soil nutrients. Such strains can be used effective bioinoculants farming. In this work, rhizosphere bacteria from Zea mays Arachis hypogaea plants Salem area Tamil Nadu, India, were isolated tested biochemical attributes characteristics that stimulate plant growth, such production hydrogen cyanide, ammonia (6 µg/mL), indole acetic acid (76.35 solubilizing phosphate (520 µg/mL). 16S rRNA gene sequences identified isolate Pseudomonas fluorescens with a similarity percentage 99% sp. Isolate was evaluated siderophore. Siderophore-rich supernatant using Sep Pack C18 column Amberlite-400 Resin Column ( λ max 264) produced 298 mg/L 50 siderophore, respectively. characterization purified siderophore by TLC, HPLC, FTIR, 2D-NMR analysis compound desferrioxamine, hydroxamate A pot culture experiment determined potential to improve oil photosynthetic pigments L. content. Inoculation A. hypogea seeds improved leaf length (60%), shoot (22%), root (54.68%), fresh weight (47.28%), dry (37%), number nuts (66.66) compared control (untreated seeds). This inoculation also (43.42), short (38.38%), seed (46.72%), (31.68%), carotenoid (64.40%), total chlorophyll (98.%) Bacterized showed substantial increase nodulation (61.65%) individual nodules (95.97) vis-à-vis control. results study indicated P. might utilized bioinoculant content, Arachishypogaea L., enrich

Язык: Английский

Процитировано

11

The highly effective cadmium-resistant mechanism of Pseudomonas aeruginosa and the function of pyoverdine induced by cadmium DOI

Shixue Mei,

Wan-Ping Bian,

Aijiang Yang

и другие.

Journal of Hazardous Materials, Год журнала: 2024, Номер 469, С. 133876 - 133876

Опубликована: Фев. 24, 2024

Язык: Английский

Процитировано

7

Iron acquisition strategies in pseudomonads: mechanisms, ecology, and evolution DOI Creative Commons
Rolf Kümmerli

BioMetals, Год журнала: 2022, Номер 36(4), С. 777 - 797

Опубликована: Дек. 12, 2022

Iron is important for bacterial growth and survival, as it a common co-factor in essential enzymes. Although iron very abundant the earth crust, its bioavailability low most habitats because ferric largely insoluble under aerobic conditions at neutral pH. Consequently, bacteria have evolved plethora of mechanisms to solubilize acquire from environmental host stocks. In this review, I focus on Pseudomonas spp. first present main uptake taxa, which involve direct ferrous via importers, production iron-chelating siderophores, exploitation siderophores produced by other microbial species, use compounds plants animals. second part elaborate how these affect interactions between communities, their hosts. This live diverse communities certain iron-uptake strategies might not only nutrient, but also gain relative advantages over competitors race iron. Thus, an integrative understanding acquisition eco-evolutionary dynamics they drive community level prove useful understand why spp., particular, many general, such repertoires.

Язык: Английский

Процитировано

29

Metagenomic domain substitution for the high-throughput modification of nonribosomal peptides DOI
Sarah R. Messenger, Edward M. R. McGuinniety, Luke J. Stevenson

и другие.

Nature Chemical Biology, Год журнала: 2023, Номер 20(2), С. 251 - 260

Опубликована: Ноя. 23, 2023

Язык: Английский

Процитировано

14

Novel Insights on Pyoverdine: From Biosynthesis to Biotechnological Application DOI Open Access
Filippo Dell’Anno, Giovanni Andrea Vitale, Carmine Buonocore

и другие.

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(19), С. 11507 - 11507

Опубликована: Сен. 29, 2022

Pyoverdines (PVDs) are a class of siderophores produced mostly by members the genus Pseudomonas. Their primary function is to accumulate, mobilize, and transport iron necessary for cell metabolism. Moreover, PVDs also play crucial role in microbes' survival mediating biofilm formation virulence. In this review, we reorganize information recent years regarding biosynthesis pathogenic mechanisms, since extremely valuable compounds. Additionally, summarize therapeutic applications deriving from PVDs' use focus on their as target themselves. We assess current biotechnological different sectors evaluate state-of-the-art technology relating synthetic biology tools pathway engineering. Finally, review most methods techniques capable identifying such molecules complex matrices drug-discovery purposes.

Язык: Английский

Процитировано

20

Antimicrobial activity of iron-depriving pyoverdines against human opportunistic pathogens DOI Creative Commons
Vera Vollenweider, Karoline Rehm, Clara Chepkirui

и другие.

eLife, Год журнала: 2024, Номер 13

Опубликована: Янв. 8, 2024

The global rise of antibiotic resistance calls for new drugs against bacterial pathogens. A common approach is to search natural compounds deployed by microbes inhibit competitors. Here, we show that the iron-chelating pyoverdines, siderophores produced environmental Pseudomonas spp., have strong antibacterial properties inducing iron starvation and growth arrest in screen 320 isolates used 12 human pathogens uncovered several pyoverdines with particularly high distinct chemical characteristics. most potent pyoverdine effectively reduced Acinetobacter baumannii , Klebsiella pneumoniae, Staphylococcus aureus a concentration- iron-dependent manner. Pyoverdine increased survival infected Galleria mellonella host larvae showed low toxicity host, mammalian cell lines, erythrocytes. Furthermore, experimental evolution combined whole-genome sequencing revealed limited compared an antibiotic. Thus, from strains potential become class sustainable antibacterials specific

Язык: Английский

Процитировано

4

Feature sequence-based genome mining uncovers the hidden diversity of bacterial siderophore pathways DOI Creative Commons
Shaohua Gu, Yuanzhe Shao, Karoline Rehm

и другие.

eLife, Год журнала: 2024, Номер 13

Опубликована: Май 1, 2024

Microbial secondary metabolites are a rich source for pharmaceutical discoveries and play crucial ecological functions. While tools exist to identify metabolite clusters in genomes, precise sequence-to-function mapping remains challenging because neither function nor substrate specificity of biosynthesis enzymes can accurately be predicted. Here, we developed knowledge-guided bioinformatic pipeline solve these issues. We analyzed 1928 genomes Pseudomonas bacteria focused on iron-scavenging pyoverdines as model metabolites. Our predicted 188 chemically different with nearly 100% structural accuracy the presence 94 distinct receptor groups required uptake iron-loaded pyoverdines. unveils an enormous yet overlooked diversity siderophores (151 new structures) receptors (91 groups). approach, combining feature sequence phylogenetic approaches, is extendable other microbial genera, thus emerges powerful tool reconstruct bacterial metabolism pathways based data.

Язык: Английский

Процитировано

4

A Practical Toolkit for the Detection, Isolation, Quantification, and Characterization of Siderophores and Metallophores in Microorganisms DOI Creative Commons

Ana F. R. Gomes,

Emı́lia Sousa, Diana I. S. P. Resende

и другие.

ACS Omega, Год журнала: 2024, Номер 9(25), С. 26863 - 26877

Опубликована: Июнь 11, 2024

Siderophores are well-recognized low-molecular-weight compounds produced by numerous microorganisms to acquire iron from the surrounding environments. These secondary metabolites can form complexes with other metals besides iron, forming soluble metallophores; because of that, they widely investigated in either medicinal or environmental field. One bottlenecks siderophore research is related identification new siderophores microbial sources. Herein we have compiled a comprehensive range standard and updated methodologies that been developed over past few years provide toolbox this area current researchers.

Язык: Английский

Процитировано

3