Advances in agar-based composites: A comprehensive review
Carbohydrate Polymers,
Год журнала:
2024,
Номер
346, С. 122619 - 122619
Опубликована: Авг. 19, 2024
Язык: Английский
Fermentation-Induced Metabolic Changes and Bioactive Metabolites in Laminaria japonica fermented by Bacillus subtilis
LWT,
Год журнала:
2025,
Номер
unknown, С. 117357 - 117357
Опубликована: Янв. 1, 2025
Язык: Английский
A New Phospholipase D-Producing Bacillus cereus: Taxonomy, Mutagenesis, Fermentation Optimization and Enzyme Characterization
Applied Biochemistry and Biotechnology,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 20, 2025
Язык: Английский
A New Phospholipase D-Producing Bacillus cereus: Taxonomy, Mutagenesis, Fermentation Optimization and Enzyme Characterization
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 18, 2025
Abstract
Phospholipase
D
(PLD)
is
a
valuable
enzyme
in
industrial
processes
for
converting
phosphatidylcholine
(PC)
to
phosphatidylserine
(PS).
In
this
study,
strain
of
Bacillus
cereus
was
isolated
from
soil
and
identified
through
16S
rRNA
sequencing.
To
enhance
PLD
activity,
various
mutagenesis
strategies—including
chemical
treatment,
irradiation,
their
combinations—were
employed,
resulting
four
high-activity
positive
mutants
(C-7,
I-12,
CI
7–12,
IC
13–14).
Among
these,
the
7–12
mutant
exhibited
significantly
higher
enzymatic
showing
3.12-fold
increase
(312.2%)
compared
wild-type
strain.
Fermentation
conditions
were
optimied
using
response
surface
methodology
(RSM),
achieving
activity
35
U/mL.
The
demonstrated
stability
over
wide
temperature
range
(30–60°C)
pH
(6–10),
with
half-life
128
days.
Kinetic
analysis
revealed
V
max
20.04
µmol/h
K
m
7.13
µmol/mL,
indicating
efficient
activity.
bioconversion
experiments,
PLD-enriched
fermentation
broth
catalyzed
conversion
PC
PS,
53.0%
rate
92.3%
selectivity
PS
two-phase
system.
These
findings
expand
potential
sources
underscore
its
applicability
production
biotechnological
applications.
Язык: Английский
Analysis of rhizosphere fungal diversity in lavender at different planting years based on high-throughput sequencing technology
Xia Deng,
Renzeng Shi,
Rehab O. Elnour
и другие.
PLoS ONE,
Год журнала:
2024,
Номер
19(10), С. e0310929 - e0310929
Опубликована: Окт. 3, 2024
Continuous
cropping
is
a
common
cultivation
practice
in
lavender
cultivation,
and
the
structure
of
soil
microbial
community
one
main
reasons
affecting
continuous
disorder
lavender;
however,
relationship
between
number
years
inter-root
composition
has
not
yet
been
investigated;
using
Illumina
high-throughput
sequencing
we
detected
fungal
rhizosphere
under
1
(L1),
3
(L3),
5
(L5)
0
(L0)
years'
Yili,
Xinjiang
China.
The
results
showed
that
with
extension
planting
years,
physical-chemical
characteristics
shifted,
diversity
communities
shrank,
abundance
richness
species
decreased
then
increased,
phylogenetic
varied
greatly.
At
phylum
level,
dominant
phyla
were
Ascomycetes,
Basidiomycetes,
etc.
genus
genera
Gibberella,
Mortierella,
etc,
whose
absolute
all
increased
increasing
(P
<
0.05);
redundancy
analysis
thesoil
physicochemical
significantly
correlated
bacterial
genera.
FUN
Guild
prediction
six
groups
plant
pathogens
saprotrophs
changed
0.05),
amount
harmful
bacteria
while
arbuscular
mycorrhizal
fungui
(AMF)
decreased,
leading
to
obstacle
lavender.
findings
this
study
provida
theoretical
foundation
for
management
prevention
fungus-related
diseases
Язык: Английский
Biological production and simulated moving bed purification of streptothricin F from food waste digestate
Green Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Globally,
a
significant
amount
of
food
waste
is
generated
annually,
representing
considerable
potential
resource.
Язык: Английский
Comparative Analysis of Volatile Components in Chi-Nan and Ordinary Agarwood Aromatherapies: Implications for Sleep Improvement
Pharmaceuticals,
Год журнала:
2024,
Номер
17(9), С. 1196 - 1196
Опубликована: Сен. 11, 2024
Agarwood,
a
precious
traditional
medicinal
herb
and
fragrant
material,
is
known
for
its
sedative
sleep-improving
properties.
This
study
explores
the
mechanisms
underlying
aromatherapy
effects
of
Chi-Nan
agarwood
ordinary
in
improving
sleep.
Using
combination
gas
chromatography–mass
spectrometry
(GC-MS),
network
pharmacology,
molecular
docking
techniques,
we
identified
c
ompared
chemical
compositions
potential
targets
both
types
agarwood.
The
GC-MS
analysis
detected
87
volatile
components
across
six
aromatherapy,
with
51
shared
between
agarwood,
while
each
type
also
had
18
unique
components.
Ordinary
was
found
to
be
richer
sesquiterpenes
small
aromatic
molecules,
whereas
contained
higher
levels
chromones.
These
differences
composition
are
likely
responsible
distinct
observed
two
Through
100,
65,
47
non-repetitive
target
genes
related
sleep
improvement
were
by
(CSBTs),
common
(CUCMs),
(CUCNs),
respectively.
constructed
protein–protein
interaction
(PPI)
networks
revealed
that
key
such
as
MAOA,
MAOB,
SLC6A4,
ESR1
involved
aromatherapy.
Molecular
further
confirmed
strong
binding
affinities
major
active
components,
5-Isopropylidene-6-methyldeca-369-trien-2-one
2-(2-Phenylethyl)chromone,
these
core
targets.
results
suggest
enhances
quality
through
hormonal
neurotransmitter
pathways,
more
deeply
mediating
regulation,
predominantly
influences
particularly
those
involving
serotonin
GABA.
provides
valuable
insights
into
therapeutic
potentials
highlighting
their
roles
offering
foundation
future
research
clinical
application
agarwood-based
Язык: Английский