Advances in agar-based composites: A comprehensive review

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 346, P. 122619 - 122619

Published: Aug. 19, 2024

Language: Английский

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Fermentation-Induced Metabolic Changes and Bioactive Metabolites in Laminaria japonica fermented by Bacillus subtilis

LWT, Journal Year: 2025, Volume and Issue: unknown, P. 117357 - 117357

Published: Jan. 1, 2025

Language: Английский

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A New Phospholipase D-Producing Bacillus cereus: Taxonomy, Mutagenesis, Fermentation Optimization and Enzyme Characterization

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

Phospholipase D (PLD) is a valuable enzyme in industrial processes for converting phosphatidylcholine (PC) to phosphatidylserine (PS). In this study, strain of Bacillus cereus was isolated from soil and identified through 16S rRNA sequencing. To enhance PLD activity, various mutagenesis strategies—including chemical treatment, irradiation, their combinations—were employed, resulting four high-activity positive mutants (C-7, I-12, CI 7–12, IC 13–14). Among these, the 7–12 mutant exhibited significantly higher enzymatic showing 3.12-fold increase (312.2%) compared wild-type strain. Fermentation conditions were optimied using response surface methodology (RSM), achieving activity 35 U/mL. The demonstrated stability over wide temperature range (30–60°C) pH (6–10), with half-life 128 days. Kinetic analysis revealed V_max 20.04 µmol/h K_m 7.13 µmol/mL, indicating efficient activity. bioconversion experiments, PLD-enriched fermentation broth catalyzed conversion PC PS, 53.0% rate 92.3% selectivity PS two-phase system. These findings expand potential sources underscore its applicability production biotechnological applications.

Language: Английский

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A New Phospholipase D-Producing Bacillus cereus: Taxonomy, Mutagenesis, Fermentation Optimization and Enzyme Characterization

Applied Biochemistry and Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: May 20, 2025

Language: Английский

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Comparative Analysis of Volatile Components in Chi-Nan and Ordinary Agarwood Aromatherapies: Implications for Sleep Improvement

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(9), P. 1196 - 1196

Published: Sept. 11, 2024

Agarwood, a precious traditional medicinal herb and fragrant material, is known for its sedative sleep-improving properties. This study explores the mechanisms underlying aromatherapy effects of Chi-Nan agarwood ordinary in improving sleep. Using combination gas chromatography–mass spectrometry (GC-MS), network pharmacology, molecular docking techniques, we identified c ompared chemical compositions potential targets both types agarwood. The GC-MS analysis detected 87 volatile components across six aromatherapy, with 51 shared between agarwood, while each type also had 18 unique components. Ordinary was found to be richer sesquiterpenes small aromatic molecules, whereas contained higher levels chromones. These differences composition are likely responsible distinct observed two Through 100, 65, 47 non-repetitive target genes related sleep improvement were by (CSBTs), common (CUCMs), (CUCNs), respectively. constructed protein–protein interaction (PPI) networks revealed that key such as MAOA, MAOB, SLC6A4, ESR1 involved aromatherapy. Molecular further confirmed strong binding affinities major active components, 5-Isopropylidene-6-methyldeca-369-trien-2-one 2-(2-Phenylethyl)chromone, these core targets. results suggest enhances quality through hormonal neurotransmitter pathways, more deeply mediating regulation, predominantly influences particularly those involving serotonin GABA. provides valuable insights into therapeutic potentials highlighting their roles offering foundation future research clinical application agarwood-based

Language: Английский

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Analysis of rhizosphere fungal diversity in lavender at different planting years based on high-throughput sequencing technology

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(10), P. e0310929 - e0310929

Published: Oct. 3, 2024

Continuous cropping is a common cultivation practice in lavender cultivation, and the structure of soil microbial community one main reasons affecting continuous disorder lavender; however, relationship between number years inter-root composition has not yet been investigated; using Illumina high-throughput sequencing we detected fungal rhizosphere under 1 (L1), 3 (L3), 5 (L5) 0 (L0) years' Yili, Xinjiang China. The results showed that with extension planting years, physical-chemical characteristics shifted, diversity communities shrank, abundance richness species decreased then increased, phylogenetic varied greatly. At phylum level, dominant phyla were Ascomycetes, Basidiomycetes, etc. genus genera Gibberella, Mortierella, etc, whose absolute all increased increasing (P < 0.05); redundancy analysis thesoil physicochemical significantly correlated bacterial genera. FUN Guild prediction six groups plant pathogens saprotrophs changed 0.05), amount harmful bacteria while arbuscular mycorrhizal fungui (AMF) decreased, leading to obstacle lavender. findings this study provida theoretical foundation for management prevention fungus-related diseases

Language: Английский

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Biological production and simulated moving bed purification of streptothricin F from food waste digestate

Green Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Globally, a significant amount of food waste is generated annually, representing considerable potential resource.

Language: Английский

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