Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Дек. 28, 2024
To
investigate
the
structural
and
functional
similarities
of
microbial
communities
in
burnt-sweetness
alcoholized
tobacco
as
a
function
distance
from
equator
their
effects
on
quality,
we
sampled
Chenzhou,
Hunan
Province,
China
Brazil
Zimbabwe,
which
are
also
burnt-sweetness-type
producing
regions,
performed
high-throughput
sequencing
bacterial
fungal
along
with
an
analysis
main
chemical
constituents
to
analyze
differences
quality
structure
communities.
The
total
nitrogen,
nicotine
starch
contents
Chenzhou
were
greater
than
those
Brazilian
Zimbabwean
tobacco,
sugar
reducing
(P
<
0.05).
alpha
diversity
indices
lower
In
ecological
networks,
bacterial–fungal
interactions
more
complex
networks
three
different
regions
dominated
by
competitive
relationships.
community
composition
was
similar
that
at
genus
phylum
level,
Sphingomonas
being
significantly
enriched
key
network
is
able
participate
degradation
polyphenols
aromatic
compounds.
Functional
microbes
related
compounds
cellulose
abundant
substances
responsible
for
better
tobacco.
conclusion,
there
structure,
flora
because
these
have
latitudinal
distributions.
This
study
provides
theoretical
support
selecting
cultivation
alcoholization
leaves.
The
air-curing
process
of
cigar
tobacco
leaves
is
typically
conducted
in
an
open
environment,
involving
the
participation
various
microorganisms.
However,
effect
microbial
communities
during
on
formation
flavor
components
remains
unclear.
Therefore,
this
study
aims
to
reveal
dynamics
and
community
changes,
explore
potential
role
process.
High-throughput
sequencing
analysis
showed
that
Pantoea,
Sphingomonas
Pseudomonas
were
dominant
bacterial
genera
process,
while
Aspergillus
was
fungal
genus.
Subsequently,
volatile
shows
alkaloids
most
important
compounds
leaves,
followed
by
esters,
alcohols
aldehydes.
Furthermore,
38
characteristic
at
different
periods
identified
based
PLS-DA
air-curing.
correlation
between
microorganisms
Pantoea
Staphylococcus
might
promote
from
browning
post-air-curing
positively
correlated
with
specific
like
phenylacetaldehyde
acetophenone.
Phoma,
Mycosphaerella,
Wallemia,
Cladosporium
as
key
influencing
formation,
they
positive
correlations
multiple
components.
These
information
enrich
our
understanding
air
curing.
There
a
complex
components,
which
may
have
great
influence
leaves.
Bacterial
higher
species
diversity
richness
air-curing,
more
characteristics
flavor,
play
roles
formation.
This
revealed
provided
guidance
for
subsequent
screening
functional
improve
stabilize
flavor.
Rapid Communications in Mass Spectrometry,
Год журнала:
2025,
Номер
39(10)
Опубликована: Фев. 18, 2025
ABSTRACT
Rationale
The
cigars
have
characteristic
flavors
in
smoke
when
compared
with
cigarettes,
and
from
various
origins
also
a
difference
taste.
However,
little
information
can
be
found
about
the
chemical
components
of
smokes
between
cigar
cigarette
as
well
cigars,
so
it
is
interesting
to
compare
their
pyrolysis
product
distribution.
Methods
leaves
were
pyrolyzed
microfluidized‐bed
reactor,
vapors
condensed
collected
using
cold
traps.
Mass
spectrometric
analysis
liquids
was
performed
utilizing
electrospray
ionization‐orbitrap
mass
spectrometry
both
positive
negative
ion
modes.
Results
spectra
condensable
products
three
tobacco
obtained
by
Orbitrap‐MS
ESI
DBE
values
(to
carbon
atom
number)
relative
distribution
nitrogen
or
oxygen
different
carefully
discussed.
Conclusion
main
relates
N
1
–N
2
class
compounds
high‐mass
range
highly
unsaturated
nitrogenated
compounds,
while
one
two
associated
C
x
H
y
O
z
5–9
30‐50
w
compounds.
Besides,
oxygenated
that
fall
into
H/C
>
O/C
<
ranges
are
for
Dominican
cigar.
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Июль 31, 2024
The
quality
of
fermented
plant
leaves
is
closely
related
to
the
interleaf
microorganisms
and
their
metabolic
activities.
In
this
experiment,
a
multi-omics
analysis
was
applied
investigate
link
between
structural
composition
phyllosphere
microbial
community
main
metabolites
during
fermentation
process.
It
found
that
whole
process
cigar
could
be
divided
into
three
stages,
in
which
Mid-Stage
most
active
period
activities
occupied
an
important
position.
Annual Research & Review in Biology,
Год журнала:
2024,
Номер
39(3), С. 48 - 57
Опубликована: Март 9, 2024
Aims:
Mildew
poses
a
significant
threat
to
tobacco
industry.
This
study
focuses
on
strain
of
Cladosporium
asperulatum
isolated
from
moldy
leaves
investigate
its
growth
under
different
temperature
and
humidity
conditions,
aiming
provide
theoretical
basis
for
local
prevention
control
strategies
against
this
fungus.
Methodology:
Potential
fungal
strains
were
obtained
utilizing
isolation
techniques
individual
colonies
cultured
PDA
medium
subsequently.
Microscopic
examination
molecular
identification
employed
determine
the
species
strains.
Isolated
was
initially
inoculated
with
varying
spore
powder
concentrations
at
water
activities
0.99,
0.95,
0.90,
0.87,
0.83,
0.77,
combined
diverse
culture
temperatures
15°C,
20°C,
25°C,
30°C,
35°C
PDA.
The
colony
area
daily
measured
by
ImageJ
software,
yield
observed
after
7-day
incubation
period
these
specified
conditions.
Results:
(1)
C.
CY-H1
has
rising
trend
increasing
time
consistent
temperatures.
Sporulation
exhibited
an
initial
increase
followed
decrease,
yet
overall
demonstrated
upward
trajectory.
maximum
value
attained
activity
0.95.
Under
identical
activity,
escalating
augmented
until
reaching
25°C.
Similarly,
production
displayed
highest
occurring
(2)
Varied
suspension
influenced
differently.
A
high
concentration
(initially
106
spores/mL)
earlier
compared
(105
low
(104
spores/mL).
(3)
did
not
thrive
conditions
35°C,
irrespective
inoculation
activity.
Conclusion:
assumes
crucial
role
as
predominant
mold
in
storage
environments.
Effective
mildew
stored
is
achieved
maintaining
exceeding
sustaining
below
0.77.
These
findings
highlight
significance
environmental
controlling
during
storage.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Июль 25, 2024
Cigar
wrapper
leaves
(CWLs)
plays
a
crucial
role
in
reflecting
cigar
overall
quality.
Originating
from
the
Qinba
region
of
China,
Fangxian
Huangjiu
(FHJ)
is
distinctive
other
varieties
Huangjiu.
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Дек. 16, 2024
Cigar
tobacco
leaves
exhibited
distinct
regional
characteristics,
and
aroma
compounds
were
the
key
substances
determining
different
style
features
of
cigars.
However,
differences
in
characteristics
mechanisms
compound
synthesis
have
not
been
fully
elucidated.
This
study
collected
filler
(FTLs)
from
5
representative
domestic
international
production
regions.
Gas
chromatography-mass
spectrometry
(GC-MS)
identified
compounds,
an
wheel
was
established
based
on
odor
activity
values
(OAV),
principal
component
analysis
(PCA)
orthogonal
partial
least
squares
discriminant
(OPLS-DA)
revealed
major
differences.
Synthesis
pathways
differential
components
further
explored
using
Kyoto
Encyclopedia
Genes
Genomes
(KEGG).
In
this
study,
56
FTLs.
Imported-FTLs
(IMP-FTLs)
contained
higher
levels
ketones
esters,
along
with
moderate
nicotine
content,
a
more
noticeable
sour
woody
aroma.
contrast,
Domestic-FTLs
(DOM-FTLs)
had
greater
distribution
aldehydes,
phenols,
neophytadiene,
presenting
prominent
bean,
burnt-sweet,
floral
Nine
including
sclareol,
5-methylfurfural,
(
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 26, 2024
Abstract
To
investigate
the
structural
and
functional
similarities
of
microbial
communities
in
burnt-sweetness
alcoholized
tobacco
as
a
function
distance
from
equator
their
effects
on
quality,
we
sampled
Chenzhou,
Hunan
Province,
China
Brazil
Zimbabwe,
which
are
also
burnt-sweetness-type
producing
regions,
performed
high-throughput
sequencing
bacterial
fungal
along
with
an
analysis
main
chemical
constituents
to
analyze
differences
quality
structure
communities.
The
total
nitrogen,
nicotine
starch
contents
Chenzhou
were
greater
than
those
Brazilian
Zimbabwean
tobacco,
sugar
reducing
(P
<
0.05).
alpha
diversity
indices
lower
In
ecological
networks,
bacterial–fungal
interactions
more
complex
networks
three
different
regions
dominated
by
competitive
relationships.
community
composition
was
similar
that
at
genus
phylum
level,
Sphingomonas
being
significantly
enriched
key
network
is
able
participate
degradation
polyphenols
aromatic
compounds.
Functional
microbes
related
compounds
cellulose
abundant
substances
responsible
for
better
tobacco.
conclusion,
there
structure,
flora
because
these
have
latitudinal
distributions.
This
study
provides
theoretical
support
selecting
cultivation
alcoholization
leaves.
