Potential effect of Wolbachia on virus restriction in the spider mite T. truncatus

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Май 29, 2025

The mite T. truncatus is a significant agricultural pest and may serve as potential vector for viral transmission. However, the virome of remains understudied. Through metatranscriptomic analyses publicly available data, we uncovered diverse range viruses associated with spider mite, including crop-infecting pathogenic species such Potato virus Y Cherry A , fourteen previously unknown across several families (e.g., Virgaviridae Dicistroviridae Kitaviridae Betaflexiviridae Nudiviridae ). Taking advantage samples under different conditions, also assessed impact biotic ( Wolbachia Spiroplasma infection) abiotic stresses (pesticide exposure temperature stress) on virome. Interestingly, appeared to restrict infections in by reducing diversity abundance, pronounced effect dicistroviruses. Surprisingly, similar observed . restriction phenotype vanishes co-infected mites. Transcriptomics analysis singly-infected mites revealed upregulation piRNA autophagy-related genes, while lipid metabolism processes-related genes were downregulated, indicating an endosymbiont-sharing mechanisms interference. Although stressors was not statistically significant, TtDV-2 absent abamectin-exposed mites, suggesting reduction diversity, heat-stressed exhibited slightly higher compared those raised at regular temperatures. Overall, our work provides detailed virome, shedding light how endosymbionts environmental factors shape dynamics offering insights management strategies.

Язык: Английский

Spiroplasma shows a Wolbachia-like effect in hampering virus replication in spider mite

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 10, 2024

Background Members of the Acari order, commonly known as mites, play a significant role agricultural pests. Among these, Tetranychidae family stands out due to its remarkable diversity, surrounding approximately 1200 species capable infesting over 4000 plant species. By feeding on tissues, these mites directly harm crops and can also serve vectors for viral pathogens, posing substantial threat global food security. In this study, we investigated unexplored virome Tetranychus truncatus exploring how biotic factors (Spiroplasma Wolbachia endosymbionts), abiotic stresses (abamectin temperature) affect virus dynamics. Results Our metatranscriptomics analyses revealed sequences related important arthropod- crop-infecting families, including plant-pathogenic Potato Y Cherry A strains fourteen new Notably, abamectin treatment correlated with absence Potato Y TtDV-2 virus, suggesting pesticide impacts diversity. Interestingly, single infections or Spiroplasma significantly decreased both diversity abundance viruses, greatest effect dicistroviruses, indicating first time potential restrict infections. Surprisingly, Wolbachia-Spiroplasma co-infection leads loss restriction effect. Wolbachia-exclusive Spiroplasma-exclusive responsive genes showed enrichment similar pathways, piRNA autophagy enriched in up-regulated genes. contrast, lipid metabolic processes were down-regulated elements. Conclusions Overall, our study describes T. virome, unveiling considerable influence microbiome, Spiroplasma, Abamectin abundance.

Язык: Английский