Spiroplasma shows a Wolbachia-like effect in hampering virus replication in spider mite
Abstract
Background
Members
of
the
Acari
order,
commonly
known
as
mites,
play
a
significant
role
agricultural
pests.
Among
these,
Tetranychidae
family
stands
out
due
to
its
remarkable
diversity,
surrounding
approximately
1200
species
capable
infesting
over
4000
plant
species.
By
feeding
on
tissues,
these
mites
directly
harm
crops
and
can
also
serve
vectors
for
viral
pathogens,
posing
substantial
threat
global
food
security.
In
this
study,
we
investigated
unexplored
virome
Tetranychus
truncatus
exploring
how
biotic
factors
(Spiroplasma
Wolbachia
endosymbionts),
abiotic
stresses
(abamectin
temperature)
affect
virus
dynamics.
Results
Our
metatranscriptomics
analyses
revealed
sequences
related
important
arthropod-
crop-infecting
families,
including
plant-pathogenic
Potato
Y
Cherry
A
strains
fourteen
new
Notably,
abamectin
treatment
correlated
with
absence
Potato
Y
TtDV-2
virus,
suggesting
pesticide
impacts
diversity.
Interestingly,
single
infections
or
Spiroplasma
significantly
decreased
both
diversity
abundance
viruses,
greatest
effect
dicistroviruses,
indicating
first
time
potential
restrict
infections.
Surprisingly,
Wolbachia-Spiroplasma
co-infection
leads
loss
restriction
effect.
Wolbachia-exclusive
Spiroplasma-exclusive
responsive
genes
showed
enrichment
similar
pathways,
piRNA
autophagy
enriched
in
up-regulated
genes.
contrast,
lipid
metabolic
processes
were
down-regulated
elements.
Conclusions
Overall,
our
study
describes
T.
virome,
unveiling
considerable
influence
microbiome,
Spiroplasma,
Abamectin
abundance.
Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 10, 2024
Language: Английский