Genome-wide association study of rice (Oryza sativa L.) inflorescence architecture

Plant Science, Год журнала: 2025, Номер unknown, С. 112382 - 112382

Опубликована: Янв. 1, 2025

Rice yield strongly depends on panicle size and architecture but the genetics underlying these traits their coordination with environmental cues through various signaling pathways have remained elusive. A genome-wide association study (GWAS) was performed to pinpoint genetic determinants for rice by analyzing 20 panicle-related using a data set consisting of 44,100 SNPs. We defined QTL windows around significant SNPs rate LD decay each chromosome used identify putative candidate genes associated trait. Using publicly available RNA-seq we analyses differentially expressed between stem functions in architecture. In total, 52 were identified, corresponding 41 unique QTLs across 12 chromosomes, most signals appearing 1 (nine SNPs), seven chromosomes 8 12. Some novel such as Ankyrin, Duf, Kinesin Brassinosteroid insensitive found be size. haplotype analysis showed that variation haplotypes qMIL2 qNSBBH21 related two traits, MIL, greatest distance nodes rachis, NSBBH, number primary branches bottom half panicle, respectively. Analysis epistatic interactions revealed marker affecting clustered traits. Several identified different first time which may explain phenotypic diversity observe our collection accessions. The could marker-assisted selection improve gene pyramiding.

Язык: Английский

Genome-wide identification of CAMTA gene family in teak (Tectona grandis) and functional characterization of TgCAMTA1 and TgCAMTA3 in cold tolerance

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 10, 2025

Calmodulin-binding transcription activator (CAMTA) proteins play significant roles in signal transduction, growth and development, as well abiotic stress responses, plants. Understanding their involvement the low-temperature response of teak is vital for revealing cold resistance mechanisms. Through bioinformatics analysis, CAMTA gene family was examined, six genes were identified teak. The encoded predicted to be located nucleus exhibited hydrophilic properties, with molecular weights ranging from 103.4 123.3 kDa isoelectric points 5.49 7.55. On basis protein sequence homology, could divided into three subgroups. Domain 3D structure analyses demonstrated that all TgCAMTA contained typical domain CaMBD binding domain, which exposed on surface. Expression analysis different tissues revealed expression roots, stems, leaves, flowers, fruits, branches. Furthermore, promoter region various cis-acting elements related light, hormone, responses. After treatment, patterns TgCAMTAs observed TgCAMTA1 showing highest level leaves compared stems. Transgenic lines carrying TgCAMTA1/3 promoter::GUS construct induction presence multiple responsive region. Subcellular localization indicated these functional predominantly nucleus. Compared wild-type Arabidopsis, TgCAMTA1/3-overexpressing Arabidopsis presented increased tolerance freezing stress, AtCOR genes. Moreover, under conditions, TgCAMTA3-overexpressing significantly elevated levels CBF signaling pathway, including AtCBF1/2/3. Our findings add existing knowledge regarding help elucidate mechanisms

Язык: Английский

Comprehensive Model of Cell-to-Cell Cytokinin Transport Reveals A Specific Mode of Cytokinin Riboside Influx

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 5, 2024

1 Abstract Ribosylated forms of plant hormones cytokinins (CKs) are the dominant CK species translocated at long distances. Their particular roles in physiology imply existence a yet uncharacterized riboside-specific membrane transport system. In this work, we report significant differences kinetics nucleobases and ribosides overall affinity membrane-bound carriers towards two forms. We show that can inhibit uptake tobacco Bright Yellow 2 cell suspensions but not vice versa, confirming system strictly recognizes ribosides. further characterize mediated by AtENT3 (EQULIBRATIVE NUCLEOSIDE TRANSPORTER 3), showing its preference trans -zeatin riboside (tZR) over isopentenyl adenosine (iPR). With molecular docking dynamics, assess interactions among side chain tZR residues Tyr61 Asp129, which conserved all AtENTs ENTs from non-plant species. Lastly, atent3 mutation affects shoot phenotype, demonstrating impact on development.

Язык: Английский