lncRNA-microRNA axis in cancer drug resistance: particular focus on signaling pathways

Medical Oncology, Год журнала: 2024, Номер 41(2)

Опубликована: Янв. 9, 2024

Язык: Английский

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Endometrial Receptivity–Lessons from “Omics”

Biomolecules, Год журнала: 2025, Номер 15(1), С. 106 - 106

Опубликована: Янв. 11, 2025

The window of implantation (WOI) is a critical phase the menstrual cycle during which endometrial lining becomes receptive and facilitates embryo implantation. Drawing on findings from various branches “omics”, including genomics, epigenomics, transcriptomics, proteomics, lipidomics, metabolomics, microbiomics, this narrative review aims to (1) discuss mechanistic insights receptivity its implication in infertility; (2) highlight advances investigations for receptivity; (3) novel diagnostic therapeutic strategies that may improve reproductive outcomes.

Язык: Английский

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Using ncRNAs as Tools in Cancer Diagnosis and Treatment—The Way towards Personalized Medicine to Improve Patients’ Health

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(16), С. 9353 - 9353

Опубликована: Авг. 19, 2022

Although the first discovery of a non-coding RNA (ncRNA) dates back to 1958, only in recent years has complexity transcriptome started be elucidated. However, its components are still under investigation and their identification is one challenges that scientists presently facing. In addition, function far from being fully understood. The portion genome indeed largest, both quantitatively qualitatively. A large fraction these ncRNAs have regulatory role either coding mRNAs or other ncRNAs, creating an intracellular network crossed interactions (competing endogenous networks, ceRNET) fine-tune gene expression health disease. alteration equilibrium among such can enough cause transition disease, but opposite equally true, leading possibility intervening based on mechanisms cure human conditions. this review, we summarize present knowledge mechanisms, illustrating how they used for disease treatment, current pitfalls, roles environmental lifestyle-related contributing factors, addition ethical, legal, social issues arising (improper) use.

Язык: Английский

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The potential regulatory role of the lncRNA-miRNA-mRNA axis in teleost fish

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Фев. 21, 2023

Research over the past two decades has confirmed that noncoding RNAs (ncRNAs), which are abundant in cells from yeast to vertebrates, no longer “junk” transcripts but functional regulators can mediate various cellular and physiological processes. The dysregulation of ncRNAs is closely related imbalance homeostasis occurrence development diseases. In mammals, ncRNAs, such as long (lncRNAs) microRNAs (miRNAs), have been shown serve biomarkers intervention targets growth, development, immunity, disease progression. regulatory functions lncRNAs on gene expression usually mediated by crosstalk with miRNAs. most predominant mode lncRNA-miRNA lncRNA-miRNA-mRNA axis, act competing endogenous (ceRNAs). Compared little attention given role mechanism axis teleost species. this review, we provide current knowledge about focusing its pathological regulation growth reproduction, skeletal muscle, immunity bacterial viral infections, other stress-related immune responses. Herein, also explored potential application aquaculture industry. These findings contribute an enhanced understanding ncRNA ncRNA-ncRNA fish biology improve productivity, health quality.

Язык: Английский

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Circulating miRNAs as a Tool for Early Diagnosis of Endometrial Cancer—Implications for the Fertility-Sparing Process: Clinical, Biological, and Legal Aspects

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(14), С. 11356 - 11356

Опубликована: Июль 12, 2023

This review article explores the possibility of developing an integrated approach to management different needs endometrial cancer (EC) patients seeking become pregnant. Life preservation woman, health baby, a precocious and—as much as possible—minimally invasive characterization and fertility parameters patient, together with concerns regarding obstetric, neonatal, adult risks children conceived via assisted reproductive techniques (ART) are all essential aspects problem be taken into consideration, yet harmonize such through concerted is still very challenging. aims illustrate main features EC how it affects normal physiology pre-menopausal women. We also focus on prospect miR-based, molecular evaluation patient status, including both early diagnosis staging and, similarly, receptivity discussing possible using single specific panel circulating miRs in thus allowing relatively fast, non-invasive testing significantly reduced margin error. Finally, ethical legal/regulatory innovative require not only risk-benefit analysis; respect for autonomy equitable care access allocation fundamental issues well.

Язык: Английский

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LINC01088 prevents ferroptosis in glioblastoma by enhancing SLC7A11 via HLTF/USP7 axis

Clinical and Translational Medicine, Год журнала: 2025, Номер 15(3)

Опубликована: Фев. 25, 2025

Glioblastoma multiforme (GBM)is a highly aggressive malignancy of the central nervous system characterized by poor survival rates. Ferroptosis, an iron-dependent cell death pathway, is promising therapeutic target for GBM. However, current treatments targeting pathways have not yielded expected results. Long noncoding RNAs (lncRNAs) been implicated in tumour proliferation, however, their role ferroptosis GBM remains underexplored. This study investigated interplay between lncRNA LINC01088 and to identify novel strategies. We conducted gain- loss-of-function studies assess impact on tumourigenesis both vitro vivo. Bioinformatics, dual-luciferase reporter assays, chromatin immunoprecipitation, RNA pulldown, mass spectrometry, immunoprecipitation (RIP), transcriptome sequencing were utilized elucidate mechanisms underlying expression its downstream effects ferroptosis. The transcription factor specificity protein 1 (SP1) was identified as promoter transcription, which facilitated progression. found inhibit promote malignancy. Mechanistically, stabilized HLTF enhancing interaction with USP7 preventing ubiquitin-mediated degradation. stabilization led upregulation SLC7A11, inhibits Rescue experiments confirmed that altering levels reversed ferroptotic phenotypes associated modulation. revealed SP1/LINC01088/HLTF/USP7/SLC7A11 axis regulates GBM, highlighting potential ferroptosis-dependent treatment. transcriptionally upregulated SP1. acts scaffold platform bind HLTF. USP7, deubiquitinating enzyme HLTF, participates inhibiting ubiquitin-proteasome degradation upregates cells inhibited.

Язык: Английский

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Circulating miRNAs as a Tool for Early Diagnosis of Endometrial Cancer – Implications for the Fertility-sparing Process: Clinical, Biological and Legal Aspects

Опубликована: Июнь 6, 2023

This review article is aimed at exploring the possibility of developing an integrated approach in management different needs endometrial cancer (EC) patients seeking to become pregnant. Life preservation woman, health baby, a precocious and – as much possible minimally invasive characterization fertility parameters patient, together with concerns regarding obstetric, neonatal, adult risks children conceived via assisted reproduction techniques (ART) are all essential aspects problem be taken into consideration, yet harmonize such through concerted still highly challenging. aims illustrate main features EC how it affects normal physiology pre-menopausal women. Then, we focus on prospect for miR-based, molecular evaluation patient status, including both early diagnosis staging and, similarly, receptivity discussing using single specific panel circulating miR thus allowing relatively fast, non-invasive way perform testing significantly reduced margin error. Finally, ethical legal/regulatory complexities arising from innovative require not only risk-benefit analysis; respect autonomy equitable care access allocation fundamental issues well.

Язык: Английский

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Non-coding RNAs in thoracic disease: Barrett’s esophagus and esophageal adenocarcinoma

Clinica Chimica Acta, Год журнала: 2025, Номер unknown, С. 120242 - 120242

Опубликована: Март 1, 2025

Язык: Английский

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Inhibition of LINC00707/miR‐223‐3p/FKBP5 axis has a protective effect on diabetic kidney disease

Journal of Diabetes Investigation, Год журнала: 2025, Номер unknown

Опубликована: Март 21, 2025

Research indicates that LINC00707 is abnormally expressed in patients suffering from diabetic kidney disease (DKD). Nevertheless, the precise role of context DKD remains enigmatic and warrants further investigation. Initially, RT-qPCR was employed to assess expression levels with DKD. Subsequently, we cultured glomerular podocytes under high-glucose conditions. The effects on cell proliferation, apoptosis, podocyte functionality (Podocin, Nephrin, CD2AP), oxidative stress (MDA SOD), inflammatory responses (IL-1β, IL-6, TNF-α) were evaluated using CCK-8, flow cytometry, RT-qPCR, ELISA assays. Finally, co-regulated alongside its downstream targets elucidate molecular mechanisms by which influences injury. upregulated patients. Furthermore, a notable inverse correlation has been observed between renal function these Upon downregulating LINC00707, an increase cellular proliferative activity, elevated Podocin, CD2AP. Concurrently, reduction associated decreased MDA, as well proinflammatory cytokines. Significantly, inhibition miR-223-3p found reverse effects. Further investigation revealed directly FK506-binding protein 5 (FKBP5). Inhibition may reduce damage during hyperglycemia targeting miR-223-3p/FKBP5.

Язык: Английский

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Preliminary Transcriptome Analysis of Long Noncoding RNA in Hypothalamic-Pituitary-Mammary Gland Axis of Dairy Cows under Heat Stress

Biomolecules, Год журнала: 2023, Номер 13(2), С. 390 - 390

Опубликована: Фев. 18, 2023

Heat stress (HS) is directly correlated to mammary gland dysfunction in dairy cows, especially summer. The hypothalamic−pituitary−mammary axis (HPM axis) plays an important role the regulation of response and lactation physiology heat−stressed cows. aim this study was explore lncRNA profile, competitive endogenous RNA (ceRNA) regulatory network hypothalamus, pituitary, tissues normal We performed sequencing (RNA−seq) identify differentially expressed (DE) lncRNAs, ceRNA established HPM−axis−related tissues. Our results showed that a total 13, 702 202 DE lncRNAs were identified glands, respectively. Of 8, 209 45 up−regulated, 5, 493 157 down−regulated. Gene ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses indicated target genes might play hormone synthesis, secretion action, apoptosis, mitogen−activated protein kinase (MAPK), AMP−activated (AMPK), mechanistic rapamycin (mTOR) signaling pathway. Moreover, associated with MAPK pathway contains 3286 lncRNA–mRNA pairs. Furthermore, prolactin, AMPK, mTOR 772 Thus, some may be involved physiological process lactation. changes expression profiles ceRNAs (lncRNA–miRNA–mRNA) are key affect cows under HS, which provide theoretical basis for molecular mechanism HS.

Язык: Английский

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