Key bacterial vaginosis-associated bacteria influence each other’s growth in biofilms on rich media and media simulating vaginal tract secretions

Biofilm, Год журнала: 2025, Номер 9, С. 100247 - 100247

Опубликована: Янв. 5, 2025

Bacterial vaginosis (BV) is a very common gynaecologic condition affecting women of reproductive age worldwide. BV characterized by depletion lactic acid-producing Lactobacillus species and an increase in strict facultative anaerobic bacteria that develop polymicrobial biofilm on the vaginal epithelium. Despite multiple decades research, etiology this infection still not clear. However, some BV-associated (BVAB) may play key role development infection, namely Gardnerella species, Prevotella bivia, Fannyhessea vaginae. In work, we aimed to characterize growth these three rich medium simulating tract secretions (mGTS). We first assessed planktonic New York City (NYCIII) mGTS observed showed distinct capacities grow two media. Surprisingly, despite ability all single-species NYCIII, triple-species consortium P. bivia was able its concentration after 48 h, as qPCR. Furthermore, when using more restrictive media, G. vaginalis only BVAB consortia. Interestingly, found NYCIII influenced cell-free supernatant (CFS) F. vaginae CFS mGTS. This antimicrobial activity appears happen due acidification Single- biofilms were then formed, each further quantified While had high capacity form both favored cultured Differences also structure formed confocal laser scanning microscopy. conclusion, while essential for incorporation other biofilm.

Язык: Английский

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Fighting polymicrobial biofilms in bacterial vaginosis

Microbial Biotechnology, Год журнала: 2023, Номер 16(7), С. 1423 - 1437

Опубликована: Апрель 12, 2023

Abstract Bacterial vaginosis (BV) is the most common cause of vaginal discharge and often associated with other health consequences mainly in pregnant women. BV described by an imbalance microbiota where strictly facultative anaerobic bacteria outgrow lactic acid‐ hydrogen peroxide‐producing Lactobacillus species. The species involved are capable to grow form a polymicrobial biofilm epithelium. treatment usually performed using broad‐spectrum antibiotics, including metronidazole clindamycin. However, these conventional treatments high recurrence rates. may have important role on outcome accounted as one factors for failure. Other possible reasons failure include presence resistant antibiotics or chance reinfection after treatment. Therefore, novel strategies increase rates been studied namely use probiotics prebiotics, acidifying agents, antiseptics, plant‐based products, transplantation, phage endolysins. Although some them still initial phase development very preliminary results, they show great perspectives application. In this review, we aimed study nature explore few alternatives

Язык: Английский

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Bacterial Vaginosis and Post-Operative Pelvic Infections

Healthcare, Год журнала: 2023, Номер 11(9), С. 1218 - 1218

Опубликована: Апрель 25, 2023

Bacterial vaginosis (BV) represents a condition in which the normal protective Lactobacilli, especially those that produce H2O2, are replaced by high quantities of facultative anaerobes, leading to gynecologic and obstetric post-operative complications. BV is an important cause gynecological adverse sequelae it could lead increased risk contracting sexually transmitted infections such as gonorrhea, genital herpes, Chlamydia, Trichomonas, human immunodeficiency virus. Herein, we reviewed bacterial its association with pelvic infections. In Obstetrics, has been associated preterm delivery, first-trimester miscarriage women undergoing vitro fertilization, premature rupture membranes, chorioamnionitis, amniotic fluid infections, postpartum postabortal endomyometritis well inflammatory disease (PID). gynecology, increases post-hysterectomy vaginal cuff cellulitis, abscess, PID. often asymptomatic, can resolve spontaneously, relapses or without treatment. The American College Obstetricians Gynecologists recommends testing for having delivery. Women symptoms should be evaluated treated. surgeries must treated reduce frequency Metronidazole clindamycin mainstays therapy. Currently, there no consensus on pre-surgery screening BV; decisions made case-by-case basis.

Язык: Английский

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Sialidases derived from Gardnerella vaginalis remodel the sperm glycocalyx and impair sperm function

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 5, 2025

Abstract Bacterial vaginosis (BV), a dysbiosis of the vaginal microbiome, affects approximately 29 percent women worldwide (up to 50% in some regions) and is associated with several adverse health outcomes including preterm birth increased incidence sexually transmitted infection (STI). BV-associated bacteria, such as Gardnerella vaginalis Prevotella timonensis, damage mucosa through activity sialidase enzymes that remodel epithelial glycocalyx degrade mucin glycoproteins. This creates an inflammatory environment which likely contributes outcomes. However, whether glycolytic also sperm during their transit reproductive tract has not yet been determined. Here, we show sialidase-mediated remodeling human increases susceptibility within female tract. In particular, report desialylated demonstrate complement lysis agglutination, well decreased cervical mucus. Our results mechanism by sialidases may affect survival function potentially contribute infertility. Significance statement Sperm surface glycans are crucial for immune processes We found derived from bacteria resulting aberrant mucus trapping, complement-mediated cytolysis sperm. These implicate potential Classification: Biological Sciences, Immunology Inflammation,

Язык: Английский

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Effects of bacterial vaginosis treatment during pregnancy on maternal-fetal outcome: A systematic review and network meta-analysis

European Journal of Obstetrics & Gynecology and Reproductive Biology, Год журнала: 2025, Номер 307, С. 175 - 183

Опубликована: Фев. 8, 2025

Язык: Английский

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Alternative treatment strategies for bacterial vaginosis that include the use of lactic acid as a potential solution to the problem of antibiotic resistance

V F Snegirev Archives of Obstetrics and Gynecology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 24, 2025

BACKGROUND. Bacterial vaginosis is a common infectious non-inflammatory vaginal disease that increases the likelihood of contracting sexually transmitted infections, which has negative impact on perinatal outcomes and generally reduces quality life. Given low long-term efficacy antibiotic therapy, as well high recurrence rate side effects associated with use, there need to find alternative approaches its treatment. AIM. To evaluate tolerability comprehensive two-stage treatment BV including clindamycin or dequalinium chloride lactic acid in women reproductive age. METHODS. An open randomized clinical study was carried out, involved 54 aged 18 45 years diagnosis bacterial confirmed by Amsel's criteria. The participants were randomly assigned into three groups: 17 from first group used acid, 20 second combination third acid. Treatment evaluated after 14 days using Three months completion treatment, complaints assessed pH measured. RESULTS. During study, it noted two weeks whitish-gray discharge ceased all groups. In group, where only used, continued patients. Positive dynamics change secretions observed groups both end most noticeable effect together persisted group. CONCLUSION. conducted effectiveness However, demonstrated more stable results short long term, making this method non-antibacterial therapy preferable context growing problem resistance.

Язык: Английский

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The association between global and prime diet quality scores and the risk of bacterial vaginosis: a secondary analysis of case-control study

Journal of Health Population and Nutrition, Год журнала: 2025, Номер 44(1)

Опубликована: Фев. 28, 2025

The present aimed to examine the relationship between Global Diet Quality (GDQ) and Prime (PDQ) scores likelihood of bacterial vaginosis (BV) among women. This case-control study was conducted patients referred a gynecological clinic in Tehran using convenience sampling method. All participants were examined by gynecologist rule out BV based on presence three or four Amsel criteria. A valid semi-quantitative food frequency questionnaire (FFQ) containing 168 items used estimate participants' dietary intake. To calculate GDQ score, 25 groups considered, while 21 for PDQ daily consumption (in grams). statistical analysis performed SPSS, association odds evaluated binary logistic regression. After adjusting age, energy intake, fat BMI, physical activity, familial history BV, pregnancy history, menstrual cycle, smoking number sexual partners previous month, significant associations remained highest tertile total (odds ratio (OR) = 0.219, confidence interval (CI) 95%: 0.101–0.475) positive score (OR 0.235, CI 0.103–0.533), as well 0.277, 0.131–0.583) healthy 0.397, 0.185–0.854) with compared first tertile. high diet quality, indicated scores, associated decreased risk BV. These findings suggest that intervention may be viable strategy prevention management

Язык: Английский

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Oral microbiome and preterm birth

Frontiers in Medicine, Год журнала: 2023, Номер 10

Опубликована: Авг. 7, 2023

The etiology of preterm birth (PTB) is heterogeneous and not yet well known. Maternal periodontal disease has been investigated for decades a known risk factor adverse pregnancy outcomes. However, no particular bacterial species or higher taxonomic order found as causative PTB, leading to studies the whole oral microbiome. In determine if how composition microbiome associated with we performed large case-control study including women term (TB) PTB.We compared microbiomes in PTB TB, examine differences microbial richness, diversity, differential abundance specific taxa. We obtained swab samples from 152 Caucasian pregnant who were classified either (≤36 6/7 weeks, n = 61) TB (≥38 0/7 91) exclusion any other major medical obstetric conditions. these characterized by 16S ribosomal RNA (rRNA) gene sequencing V3-V4 region on MiSeq platform.The dominant microorganisms at phylum level all regardless week outcomes belonging Firmicutes, Proteobacteria, Bacteroidetes, Fusobacteria, Actinobacteria. phyla Firmicutes Bacteroidetes relatively more abundant than while Proteobacteria was less prevalent PTB. At genus level, Veillonella, Prevotella, Capnocytophaga enriched many members genera could be resolved Veillonella massillensis shown increased group.We identified maternal being independently clinically apparent infection, uterine anomalies, complications, placenta previa, placental abruption. clarification role those taxa merits further research.

Язык: Английский

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Beyond the Gut, Emerging Microbiome Areas of Research: A Focus on Early-Life Microbial Colonization

Microorganisms, Год журнала: 2023, Номер 11(2), С. 239 - 239

Опубликована: Янв. 18, 2023

Undoubtedly, the human body harbors trillions of microbes different kinds performing various physiological activities, such as priming immune system, influencing host metabolism, and improving health by providing important metabolites short-chain fatty acids. Although gut is considered “microbial organ” our it hosts most microbes, there are present in other anatomical locations differing numbers type. Research has shown presence utero, sparking a debate on “sterile womb” concept, much scope for more work this area. It to understand early-life microbiome colonization, which role developmental origins disease later life. Moreover, seminal studies have indicated beyond gut, example, adipose tissue liver. However, still unclear what exact source these their roles disease. In review, we appraise discuss emerging areas research metabolic health. Further, review importance genital microbial interactions.

Язык: Английский

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Vaginal Microbiota Molecular Profiling in Women with Bacterial Vaginosis: A Novel Diagnostic Tool

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(21), С. 15880 - 15880

Опубликована: Ноя. 1, 2023

Bacterial vaginosis (BV) is a most common microbiological syndrome. Multiplex next-generation sequencing (NGS) or molecular tests allow complete and accurate vaginal microbiota profiling in order to determine the primary causative agent. Due high costs limited availability of NGS, multiplex real-time PCR draws more attention. The present study aimed evaluate microbial composition dominant lactobacilli species non-pregnant women with bacterial using RT-PCR test its diagnostic significance. In total, 331 complaining discharge were included. BV was confirmed upon clinical examination Nugent criteria. A carried out new Femoflor test, which identifies opportunistic bacteria, STD pathogens, some viruses. According results, rate significantly reduced BV-affected patients when compared healthy women. Moreover, L. crispatus decreases, while iners remains high. Among obligate anaerobic Gardnerella vaginalis prevalent BV. demonstrated sensitivity specificity for diagnosing allows identification infection intermediate microbiota, as well Thus, application can be effectively used evaluation BV, addition, this may an alternative Amsel criteria scoring method

Язык: Английский

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