Recent applications of plant cell culture technology in cosmetics and foods

Engineering in Life Sciences, Год журнала: 2020, Номер 21(3-4), С. 68 - 76

Опубликована: Дек. 18, 2020

Abstract Plants have been used as the main source of phytochemicals with nutritional, medicinal, cultural and cosmetic applications since times immemorial. Nowadays, achieving sustainable development, global climate change, restricted access to fresh water, limited food supply growing energy demands are among critical challenges faced by humanity. Plant cell culture technology has potential address some these providing effective tools for phyto‐ingredients reduced energy, carbon water footprints. The aim this review is discuss recent trends in development plant technologies production plant‐derived substances application products formulations. specific technological steps requirements final discussed light advances cultivation differentiated undifferentiated vitro systems. Future prospects existing commercialization culture‐derived outlined through prism authors’ point view. We expect will encourage scientists, policymakers business enterprises join efforts speeding‐up mass popularization an eco‐friendly alternative method additives products.

Язык: Английский

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Synthetic biology of plant natural products: From pathway elucidation to engineered biosynthesis in plant cells

Plant Communications, Год журнала: 2021, Номер 2(5), С. 100229 - 100229

Опубликована: Авг. 9, 2021

Plant natural products (PNPs) are the main sources of drugs, food additives, and new biofuels have become a hotspot in synthetic biology.In past two decades, engineered biosynthesis many PNPs has been achieved through construction microbial cell factories.Alongside rapid development plant physiology, genetics, genetic modification techniques, hosts now expanded from single-celled microbes to complex systems.Plant biology is an emerging field that combines engineering principles with this review, we introduce recent advances biosynthetic pathway elucidation summarize progress PNP cells.Furthermore, future vision proposed.Although still long way overcoming all bottlenecks biology, ascent expected provide huge opportunity for agriculture industry.

Язык: Английский

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Plant cell cultures as heterologous bio-factories for secondary metabolite production

Plant Communications, Год журнала: 2021, Номер 2(5), С. 100235 - 100235

Опубликована: Авг. 23, 2021

Synthetic biology has been developing rapidly in the last decade and is attracting increasing attention from many plant biologists. The production of high-value plant-specific secondary metabolites is, however, limited mostly to microbes. This potentially problematic because incorrect post-translational modification proteins differences protein micro-compartmentalization, substrate availability, chaperone product toxicity, cytochrome p450 reductase enzymes. Unlike other heterologous systems, cells may be a promising alternative for metabolites. Several commercial suspension cell cultures different species have used successfully produce valuable safe, low cost, environmentally friendly manner. However, few are currently being biosynthesized using platforms, with exception natural pigment anthocyanin. Both Arabidopsis thaliana Nicotiana tabacum can developed by multiple gene transformations CRISPR-Cas9 genome editing. Given that introduction biosynthetic pathways into N. not widely used, biosynthesis foreign limited; therein lies great potential. Here, we discuss exemplary use prospects A.

Язык: Английский

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Challenges Facing CRISPR/Cas9-Based Genome Editing in Plants

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Май 18, 2022

The development of plant varieties with desired traits is imperative to ensure future food security. revolution genome editing technologies based on the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9) system has ushered in a new era breeding. Cas9 and single-guide RNA (sgRNA) form an effective targeting complex locus or loci interest, enabling all plants high accuracy efficiency. Therefore, CRISPR/Cas9 can save both time labor relative what typically associated traditional breeding methods. However, despite improvements gene editing, several challenges remain that limit application CRISPR/Cas9-based plants. Here, we focus four issues relevant editing: (1) organelle editing; (2) transgene-free (3) virus-induced (4) recalcitrant elite crop inbred lines. This review provides up-to-date summary state CRISPR/Cas9-mediated will push this technique forward.

Язык: Английский

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Plants in vitro propagation with its applications in food, pharmaceuticals and cosmetic industries; current scenario and future approaches

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Окт. 13, 2022

Plant tissue culture technique employed for the identification and isolation of bioactive phytocompounds has numerous industrial applications. It provides potential benefits different industries which include food, pharmaceutical cosmetics. Various agronomic crops i.e., cereals, fruits, vegetables, ornamental plants forest trees are currently being used in vitro propagation. coupled with biotechnological approaches leads towards sustainable agricultural development providing solutions to major food security issues. Plants rich source phytochemicals medicinal properties rendering them useful production pharmaceuticals nutraceuticals. Furthermore, there plant compounds application cosmetics industry. In addition having moisturizing, anti‐ageing, anti‐wrinkle effects; plant-derived also possess pharmacological such as antiviral, antimicrobial, antifungal, anticancer, antioxidant, anti-inflammatory, anti-allergy characteristics. The propagation industrially significant flora is gaining attention because its several advantages over conventional methods. One this quick availability throughout year, irrespective growing season, thus opening new opportunities producers farmers. sterile or endangered can be conserved by micro Hence, an extremely efficient cost-effective biosynthetic studies bio-production, biotransformation, bioconversion compounds. However, certain limitations in-vitro regeneration system including difficulties continuous operation, product removal, aseptic conditions. For applications regenerated on a large scale, these constraints need addressed future studies.

Язык: Английский

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Engineering Considerations to Produce Bioactive Compounds from Plant Cell Suspension Culture in Bioreactors

Plants, Год журнала: 2021, Номер 10(12), С. 2762 - 2762

Опубликована: Дек. 14, 2021

The large-scale production of plant-derived secondary metabolites (PDSM) in bioreactors to meet the increasing demand for bioactive compounds treatment and prevention degenerative diseases is nowadays considered an engineering challenge due large number operational factors that need be during their design scale-up. plant cell suspension culture (CSC) has presented numerous benefits over other technologies, such as conventional whole-plant extraction, not only avoiding overexploitation species, but also achieving better yields having excellent scaling-up attributes. selection bioreactor configuration depends on intrinsic properties considerations related effect operating conditions thermodynamics, kinetics, transport phenomena, which together are essential accomplishing PDSM. To this end, review, firstly, provides a comprehensive appraisement PDSM, essentially those with demonstrated importance utilization pharmaceutical industries. Then, special attention given PDSM obtained out CSC. Finally, aspects CSC stating kinetics phenomena and, hence, viability accordingly. analysis reviewed configurations will pave way future research focused scaling up, produce high value-added

Язык: Английский

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Biosynthesis of zinc oxide nanoparticles using Phoenix dactylifera and their effect on biomass and phytochemical compounds in Juniperus procera

Scientific Reports, Год журнала: 2021, Номер 11(1)

Опубликована: Сен. 27, 2021

Abstract Biosynthesized nanoparticles have played vital role recently, as suggested to be alternative physical and chemical methods. In this study, biosynthesis of zinc oxide (ZnO NPs) were carried out using leaf extracts Phoenix dactylifera L. Zinc nitrate. The effect ZnO on biomass biochemical parameters was investigated. nanostructure characterized X-ray diffraction (XRD), transmission electron microscopy (TEM), UV–visible spectrophotometer Fourier transform infrared spectroscopy (FTIR). Which resulted in spherical shape with size ranging between 16 35 nm UV absorption beak at 370.5 clear peaks functional groups. impact different concentrations (0.0 mg/L, 80 mg/L 160 mg/L) biosynthesized bioactive compounds production Juniperus procera vitro results showed that, NPs (80 boosted the growth J. Procera significantly compared non-treated plants vitro. highest concentration (160 enhanced plant beginning period, one month later shoots became yellow callus turned brownish. Moreover, influence phytochemical examined GC–MS analysis. differences among treatments recoded . Overall, substantially improved increasing such chlorophyll a, total phenolic flavonoids contents, besides protein and, SOD, CAT APX activity. might induced some well inhibit.

Язык: Английский

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Current status and future prospects in cannabinoid production through in vitro culture and synthetic biology

Biotechnology Advances, Год журнала: 2022, Номер 62, С. 108074 - 108074

Опубликована: Дек. 5, 2022

Язык: Английский

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In Vitro Technology in Plant Conservation: Relevance to Biocultural Diversity

Plants, Год журнала: 2022, Номер 11(4), С. 503 - 503

Опубликована: Фев. 12, 2022

Plant diversity is critical to the functioning of human societies, and evidence shows that plant conservation success driven by integrative approaches include social biological factors. Plants have a unique capacity reproduce asexually, propagation practices can yield large numbers plantlets. These plantlets be used in several ways fulfil goals including repopulation regions with declining densities threatened species hold cultural meaning. However, potential vitro technologies plants meaning understudied. In this paper we focus upon roles relevant biocultural environments provide an overview knowledge gaps at interface traditionally, those meaningful Indigenous Peoples. We conclude powerful tools if they are deployed manner respectful socio-cultural context which play role, but further research needed regard. suggest epistemological points facilitate future research.

Язык: Английский

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Genetic Manipulation and Bioreactor Culture of Plants as a Tool for Industry and Its Applications

Molecules, Год журнала: 2022, Номер 27(3), С. 795 - 795

Опубликована: Янв. 25, 2022

In recent years, there has been a considerable increase in interest the use of transgenic plants as sources valuable secondary metabolites or recombinant proteins. This facilitated by advent genetic engineering technology with possibility for direct modification expression genes related to biosynthesis biologically active compounds. A wide range research projects have yielded number efficient plant systems that produce specific Furthermore, bioreactors allows production be increased industrial scales, which can quickly and cheaply deliver large amounts material short time. The resulting function small factories, many them are targeted at operation patented. review paper summarizes key last ten years regarding small, green biofactories bioreactor-based proteins; it simultaneously examines proteins on an scale presents current state available patents field.

Язык: Английский

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