Consensus genomic regions associated with multiple abiotic stress tolerance in wheat and implications for wheat breeding

Scientific Reports, Год журнала: 2022, Номер 12(1)

Опубликована: Авг. 11, 2022

In wheat, a meta-analysis was performed using previously identified QTLs associated with drought stress (DS), heat (HS), salinity (SS), water-logging (WS), pre-harvest sprouting (PHS), and aluminium (AS) which predicted total of 134 meta-QTLs (MQTLs) that involved at least 28 consistent stable MQTLs conferring tolerance to five or all six abiotic stresses under study. Seventy-six out the 132 physically anchored were also verified genome-wide association studies. Around 43% had genetic physical confidence intervals less than 1 cM 5 Mb, respectively. Consequently, 539 genes in some selected providing 6 stresses. Comparative analysis underlying four RNA-seq based transcriptomic datasets unravelled 189 differentially expressed included 11 most promising candidate common among different datasets. The promoter showed promoters these include many responsiveness cis-regulatory elements, such as ARE, MBS, TC-rich repeats, As-1 element, STRE, LTR, WRE3, WUN-motif others. Further, overlapped 34 known genes. addition, numerous ortho-MQTLs maize, rice genomes discovered. These findings could help fine mapping gene cloning, well marker-assisted breeding for multiple tolerances wheat.

Язык: Английский

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Genome-wide association study unravels genomic regions associated with chlorophyll fluorescence parameters in wheat (Triticum aestivum L.) under different sowing conditions

Plant Cell Reports, Год журнала: 2023, Номер 42(9), С. 1453 - 1472

Опубликована: Июнь 20, 2023

Язык: Английский

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Fashion meets science: how advanced breeding approaches could revolutionize the textile industry

Critical Reviews in Biotechnology, Год журнала: 2024, Номер 44(8), С. 1653 - 1679

Опубликована: Март 7, 2024

Natural fibers have garnered considerable attention owing to their desirable textile properties and advantageous effects on human health. Nevertheless, natural lag behind synthetic in terms of both quality yield, as these attributes are largely genetically determined. In this article, a comprehensive overview the fiber production landscape over last 10 years is presented, with particular focus role scientific breeding techniques improving traits key crops like cotton, hemp, ramie, flax. Additionally, article delves into cutting-edge genomics-assisted techniques, including QTL mapping, genome-wide association studies, transgenesis, genome editing, potential enhancing crops. A user-friendly compendium 11226 available QTLs significant marker-trait associations derived from 136 associated diverse furnished. Furthermore, applications transcriptomics pivotal crops, elucidating distinct genes implicated augmenting investigated. information 11257 candidate/characterized or cloned sourced various emphasizing development high-quality collated. review sheds light current progress marker-assisted selection for each crop, providing detailed insights improved cultivars released different conclusion, it asserted that application modern tools holds tremendous catalyzing transformative shift industry.

Язык: Английский

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Deciphering the genetic landscape of seedling drought stress tolerance in wheat (Triticum aestivum L.) through genome-wide association studies

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Март 4, 2024

Wheat is an important cereal crop constrained by several biotic and abiotic stresses including drought stress. Understating the effect of stress genetic basis tolerance to develop resilient, high-yielding wheat cultivars. In this study, we investigated effects on seedling characteristics in association panel consisting 198 germplasm lines. Our findings revealed that had a detrimental all under investigation with maximum shoot length (50.94% reduction) minimum germination percentage (7.9% reduction). To gain deeper understanding, conducted genome-wide analysis using 12,511 single nucleotide polymorphisms (SNPs), which led identification 39 marker-trait associations (MTAs). Of these MTAs, 13 were particularly noteworthy as they accounted for >10% phenotypic variance LOD score >5. These high-confidence MTAs further utilized extract 216 candidate gene (CGs) models within 1 Mb regions. Gene annotation functional characterization identified 83 CGs relevance genes encoded WD40 repeat domain, Myb/SANT-like WSD1-like BTB/POZ Protein kinase Cytochrome P450, Leucine-rich domain superfamily, BURP Calmodulin-binding protein60, Ubiquitin-like etc. Findings from study hold significant promise breeders provide direct assistance selecting lines harboring favorable alleles improved tolerance. Additionally, SNPs will enable marker-assisted selection potential genomic regions associated enhanced wheat.

Язык: Английский

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Iron biofortification in wheat: Past, present, and future

Current Plant Biology, Год журнала: 2024, Номер 38, С. 100328 - 100328

Опубликована: Фев. 12, 2024

Iron (Fe) deficiency is a pressing global health concern, particularly affecting vulnerable groups like women and children in resource-limited areas. Addressing this challenge requires innovative solutions, biofortified crops, Fe-enriched wheat, can offer sustainable solution to improve nutrition cereal-based diets. While conventional breeding methods have yielded competitive Fe-biofortified wheat varieties across various nations, the imminent challenges securing food nutritional security for future necessitate delicate balance: maintaining genetic progress grain yield while concurrently elevating Fe content. Despite substantial strides elucidating intricacies of homeostasis, there remains knowledge gap, especially context similar crop species. It paramount gain comprehensive understanding hurdles impeding enrichment plant tissues delve into diverse mechanisms governing uptake, translocation, transport, storage within wheat. To surmount these challenges, researchers explored multitude strategies, including mutagenesis, QTL mapping, meta-QTL analysis, GWAS, transgenesis, genome editing. Furthermore, harnessing potential microorganisms, engineered endophytes coupled with genes associated accumulation, emerges as promising pragmatic tool augmenting biofortification This review underscores significant advancements made unravelling genomic aspects accumulation also delineating research directions field. By synergistically deploying multifaceted approaches, scientists hold develop characterized by enhanced content, improved bioavailability, reduced anti-nutritional factors. Such innovations play pivotal role advancing outcomes populations reliant on wheat-based diets, resource-scarce regions.

Язык: Английский

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Unlocking the hidden variation from wild repository for accelerating genetic gain in legumes

Frontiers in Plant Science, Год журнала: 2022, Номер 13

Опубликована: Ноя. 9, 2022

The fluctuating climates, rising human population, and deteriorating arable lands necessitate sustainable crops to fulfil global food requirements. In the countryside, legumes with intriguing but enigmatic nitrogen-fixing abilities thriving in harsh climatic conditions promise future security. However, breaking yield plateau achieving higher genetic gain are unsolved problems of legume improvement. Present study gives emphasis on 15 important crops, i.e., chickpea, pigeonpea, soybean, groundnut, lentil, common bean, faba cowpea, lupin, pea, green gram, back horse moth rice some forage legumes. We have given an overview world India’s area, production, productivity trends for all from 1961 2020. Our review article investigates importance gene pools wild relatives broadening base through pre-breeding alien introgression. also discussed integrating genomics, phenomics, speed breeding, engineering genome editing tools improvement programmes. Overall, breeding may undergo a paradigm shift once genomics conventional integrated near future.

Язык: Английский

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Strategies for accelerating genetic gains in crop plants: special focus on speed breeding

Physiology and Molecular Biology of Plants, Год журнала: 2022, Номер 28(10), С. 1921 - 1938

Опубликована: Окт. 1, 2022

Язык: Английский

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2Gs and plant architecture: breaking grain yield ceiling through breeding approaches for next wave of revolution in rice ( Oryza sativa L.)

Critical Reviews in Biotechnology, Год журнала: 2022, Номер 44(1), С. 139 - 162

Опубликована: Сен. 29, 2022

Rice is a principal food crop for more than half of the global population. Grain number and grain weight (2Gs) are two complex traits controlled by several quantitative trait loci (QTLs) considered most critical components yield enhancement in rice. Novel molecular biology QTL mapping strategies can be utilized dissecting genetic architecture these traits. Discovering valuable genes/QTLs associated with 2Gs hidden rice genome utilizing them breeding programs may bring revolution production. Furthermore, positional cloning functional characterization identified genes QTLs aid understanding mechanisms underlying In addition, knowledge modern genomic tools aids nature plant panicle architecture, which enhances their photosynthetic activity. researchers continue to combine important component (including ceiling) tools, such as marker-assisted selection (MAS), haplotype-based breeding, allele mining. Physical co-localization

Язык: Английский

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Meta‐QTL s and haplotypes for efficient zinc biofortification of rice

The Plant Genome, Год журнала: 2023, Номер 16(4)

Опубликована: Март 10, 2023

Biofortification of rice with improved grain zinc (Zn) content is the most sustainable and cost-effective approach to address Zn malnutrition in Asia. Genomics-assisted breeding using precise consistent quantitative trait loci (QTLs), genes, haplotypes can fast-track development biofortified varieties. We conducted meta-analysis 155 QTLs reported from 26 different studies. Results revealed 57 meta-QTLs a significant reduction 63.2% 80% number confidence interval QTLs, respectively. Meta-quantitative (MQTLs) regions were found be enriched diverse metal homeostasis genes; at least 11 MQTLs colocated 20 known major genes involved production root exudates, uptake, transport, partitioning, loading into grains rice. These differentially expressed vegetative reproductive tissues, complex web interactions observed among them. identified superior their combinations for nine candidate (CGs), frequency allelic effects varied subgroups. The high phenotypic variance, CGs, our study are useful an efficient biofortification ensure as essential component all future varieties through mainstreaming breeding.

Язык: Английский

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Comprehensive meta-QTL analysis for dissecting the genetic architecture of stripe rust resistance in bread wheat

BMC Genomics, Год журнала: 2023, Номер 24(1)

Опубликована: Май 12, 2023

Yellow or stripe rust, caused by the fungus Puccinia striiformis f. sp. tritici (Pst) is an important disease of wheat that threatens production. Since developing resistant cultivars offers a viable solution for management, it essential to understand genetic basis rust resistance. In recent years, meta-QTL analysis identified QTLs has gained popularity as way dissect architecture underpinning quantitative traits, including resistance.Systematic involving 505 from 101 linkage-based interval mapping studies was conducted resistance in wheat. For this purpose, publicly available high-quality maps were used create consensus linkage map 138,574 markers. This project and conduct analysis. A total 67 meta-QTLs (MQTLs) which refined 29 high-confidence MQTLs. The confidence (CI) MQTLs ranged 0 11.68 cM with mean 1.97 cM. physical CI 24.01 Mb, ranging 0.0749 216.23 Mb per MQTL. As many 44 colocalized marker-trait associations SNP peaks associated Some also included following major genes- Yr5, Yr7, Yr16, Yr26, Yr30, Yr43, Yr44, Yr64, YrCH52, YrH52. Candidate gene mining 1,562 models. Examining these models differential expressions yielded 123 differentially expressed genes, 59 most promising CGs. We studied how genes tissues at different phases development.The study may facilitate marker-assisted breeding Information on markers flanking can be utilized genomic selection increase prediction accuracy candidate enhancing against after vivo confirmation/validation using one more methods: cloning, reverse methods, omics approaches.

Язык: Английский

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