Genome-wide identification, structural characterization and gene expression analysis of the WRKY transcription factor family in pea (Pisum sativum L.)

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Фев. 16, 2024

Abstract Background The WRKY gene family is one of the largest families transcription factors in higher plants, and play important roles plant growth development as well response to abiotic stresses; however, pea has not been systematically reported. Results In this study, 89 genes were identified named according random distribution PsWRKY on seven chromosomes. was found have nine pairs tandem duplicates 19 segment duplicates. Phylogenetic analyses 60 Arabidopsis proteins performed determine their homology, PsWRKYs classified into subfamilies. Analysis physicochemical properties, motif composition, structure WRKYs revealed significant differences properties within family; protein-conserved motifs highly conserved among To further investigate evolutionary relationships family, we constructed comparative syntenic maps with representative monocotyledonous dicotyledonous plants that it most recently homologous families. Cis-acting element analysis can respond hormones, such abscisic acid (ABA), indole-3-acetic (IAA), gibberellin (GA), methyl jasmonate (MeJA), salicylic (SA). Further expression 14 from different subfamilies tissues fruit developmental stages, under five hormone treatments, patterns suggesting may physiological functions hormones. Conclusions for first time investigated evolution, patterns, providing a theoretical basis future studies functional characterization during development.

Язык: Английский

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Genome-wide association study unravels genomic regions associated with chlorophyll fluorescence parameters in wheat (Triticum aestivum L.) under different sowing conditions

Plant Cell Reports, Год журнала: 2023, Номер 42(9), С. 1453 - 1472

Опубликована: Июнь 20, 2023

Язык: Английский

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Fashion meets science: how advanced breeding approaches could revolutionize the textile industry

Critical Reviews in Biotechnology, Год журнала: 2024, Номер 44(8), С. 1653 - 1679

Опубликована: Март 7, 2024

Natural fibers have garnered considerable attention owing to their desirable textile properties and advantageous effects on human health. Nevertheless, natural lag behind synthetic in terms of both quality yield, as these attributes are largely genetically determined. In this article, a comprehensive overview the fiber production landscape over last 10 years is presented, with particular focus role scientific breeding techniques improving traits key crops like cotton, hemp, ramie, flax. Additionally, article delves into cutting-edge genomics-assisted techniques, including QTL mapping, genome-wide association studies, transgenesis, genome editing, potential enhancing crops. A user-friendly compendium 11226 available QTLs significant marker-trait associations derived from 136 associated diverse furnished. Furthermore, applications transcriptomics pivotal crops, elucidating distinct genes implicated augmenting investigated. information 11257 candidate/characterized or cloned sourced various emphasizing development high-quality collated. review sheds light current progress marker-assisted selection for each crop, providing detailed insights improved cultivars released different conclusion, it asserted that application modern tools holds tremendous catalyzing transformative shift industry.

Язык: Английский

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Deciphering the genetic landscape of seedling drought stress tolerance in wheat (Triticum aestivum L.) through genome-wide association studies

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Март 4, 2024

Wheat is an important cereal crop constrained by several biotic and abiotic stresses including drought stress. Understating the effect of stress genetic basis tolerance to develop resilient, high-yielding wheat cultivars. In this study, we investigated effects on seedling characteristics in association panel consisting 198 germplasm lines. Our findings revealed that had a detrimental all under investigation with maximum shoot length (50.94% reduction) minimum germination percentage (7.9% reduction). To gain deeper understanding, conducted genome-wide analysis using 12,511 single nucleotide polymorphisms (SNPs), which led identification 39 marker-trait associations (MTAs). Of these MTAs, 13 were particularly noteworthy as they accounted for >10% phenotypic variance LOD score >5. These high-confidence MTAs further utilized extract 216 candidate gene (CGs) models within 1 Mb regions. Gene annotation functional characterization identified 83 CGs relevance genes encoded WD40 repeat domain, Myb/SANT-like WSD1-like BTB/POZ Protein kinase Cytochrome P450, Leucine-rich domain superfamily, BURP Calmodulin-binding protein60, Ubiquitin-like etc. Findings from study hold significant promise breeders provide direct assistance selecting lines harboring favorable alleles improved tolerance. Additionally, SNPs will enable marker-assisted selection potential genomic regions associated enhanced wheat.

Язык: Английский

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Drought and heat stress: insights into tolerance mechanisms and breeding strategies for pigeonpea improvement

Planta, Год журнала: 2024, Номер 259(5)

Опубликована: Апрель 15, 2024

Язык: Английский

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Adaptive responses of large-seeded lentils across diverse Indian climates

Heliyon, Год журнала: 2025, Номер 11(3), С. e42184 - e42184

Опубликована: Янв. 23, 2025

A comprehensive understanding of crop responses to diverse environments is essential for designing efficient breeding programs. Currently, such insights are lacking large-seeded lentils under Indian climatic conditions. Given that, this study aimed assess the impacts conditions on phenological developments and yield lentils. This also determine genotype-by-environment interaction (GEI), identify mega-environments, ideal testing locations enhance efficiency national For this, sets lentil genotypes were tested across multiple spanning three agroclimatic zones India: North Hill Zone (NHZ), Western Plain (NWPZ), Central (CZ). Substantial variability was observed in days flowering (53-147 days), maturity duration (87-200 reproductive period (33-65 grain (0.77-2.18 t ha-1), 100-seed weight (2.3-4.5 g) locations. Environment factors dominant contributor (58 %), followed by GEI (28 with minimal genotypic effect (8 %). The CZ exhibited higher mean yields (1.47 ha-1) intra-zonal variations, while NHZ had a lower (1.04 ha-1). Low-temperature conditions, particularly maximum temperatures during periods (TMAXF, TMAXRP), low cumulative heat units associated reduced yields. Stepwise regression analysis identified growing degree-days vegetative (GDDV) NWPZ, TMAXRP CZ, as important yield-determining factors. Extended negatively impacted yield, whereas significant positive correlation between (r = +0.347, p < 0.001). Grain quadratic response weight, highest achieved at an optimal seed 3.12 g. GGE biplot "Which Won Where" two distinct Durgapura Sagar (CZ), Pantnagar Gurdaspur (NWPZ) emerged 'ideal' sites high representativeness discriminatory scaling. findings underscore influence environmental productivity emphasizing need region-specific approaches mega-environment scale. Additionally, highlights importance focusing stable phenology (photo-thermo insensitivity) traits broader adaptability stability.

Язык: Английский

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Breeding for Biotic Stress Resistance in Pea

Agriculture, Год журнала: 2023, Номер 13(9), С. 1825 - 1825

Опубликована: Сен. 18, 2023

Pea (Pisum sativum) stands out as one of the most significant and productive cool-season pulse crops cultivated worldwide. Dealing with biotic stresses remains a critical challenge in fully harnessing pea’s potential productivity. As such, dedicated research developmental efforts are necessary to make use omic resources advanced breeding techniques. These approaches crucial facilitating rapid timely development high-yielding varieties that can tolerate resist multiple stresses. The availability genomic tools, such comprehensive genetic maps reliable DNA markers, holds immense promise for integrating resistance genes from diverse sources. This integration helps accelerate gains pea crops. review provides an overview recent accomplishments resource peas. It also covers inheritance controlling various stress responses, control pathogenesis disease-causing organisms, mapping genes/QTLs, well transcriptomic proteomic advancements. By combining conventional modern omics-enabled strategies, be significantly enhanced.

Язык: Английский

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Meta‐analysis of the quantitative trait loci associated with agronomic traits, fertility restoration, disease resistance, and seed quality traits in pigeonpea (Cajanus cajan L.)

The Plant Genome, Год журнала: 2023, Номер 16(3)

Опубликована: Июнь 16, 2023

Abstract A meta‐analysis of quantitative trait loci (QTLs), associated with agronomic traits, fertility restoration, disease resistance, and seed quality traits was conducted for the first time in pigeonpea ( Cajanus cajan L.). Data on 498 QTLs collected from 9 linkage mapping studies (involving 21 biparental populations). Of these 498, 203 were projected onto “PigeonPea_ConsensusMap_2022,” saturated 10,522 markers, which resulted prediction 34 meta‐QTLs (MQTLs). The average confidence interval (CI) MQTLs (2.54 cM) 3.37 times lower than CI initial (8.56 cM). MQTLs, 12 high‐confidence (≤5 a greater number (≥5) utilized to extract 2255 gene models, 105 believed be different under study. Furthermore, eight observed overlap several marker‐trait associations or significant SNPs identified previous genome‐wide association studies. synteny ortho‐MQTL analyses among four related legumes crops, such as chickpea, pea, cowpea, French bean, led identification 117 orthologous genes 20 MQTL regions. Markers can employed MQTL‐assisted breeding well improve accuracy genomic selection pigeonpea. Additionally, may subjected fine mapping, some promising candidate serve potential targets positional cloning functional analysis elucidate molecular mechanisms underlying target traits.

Язык: Английский

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Stress adaptive plasticity from Aegilops tauschii introgression lines improves drought and heat stress tolerance in bread wheat (Triticum aestivum L.)

PeerJ, Год журнала: 2024, Номер 12, С. e17528 - e17528

Опубликована: Июнь 11, 2024

Aegilops tauchii is a D-genome donor of hexaploid wheat and potential source genes for various biotic abiotic stresses including heat drought. In the present study, we used multi-stage evaluation technique to understand effects drought on Ae. tauschii derived introgression lines (ILs). Preliminary (during stage-I) 369 ILs agronomic traits identified 59 agronomically superior ILs. second stage (stage-II), selected ( i.e. , ILs) were evaluated seedling (at 30 °C 35 °C) 20% poly-ethylene glycol; PEG) stress tolerance under growth chambers (stage-II). Heat significantly reduced vigour by 59.29 60.37 percent, respectively. Genotype × treatment interaction analysis index (STI) IL-50, IL-56, IL-68 as high-performing IL-42 IL-44 stress. It also revealed IL-50 stable stresses. Furthermore, in third (stage-III), field condition over two cropping seasons (viz., 2020–21 2021–22), which grain yield 72.79 48.70 Stability was performed identify IL-47, IL-51, IL-259 most stage-III. Tolerant with specific wider adaptability this study can serve resources genetic basis they be utilized developing high-yielding cultivars enhanced tolerance.

Язык: Английский

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Editorial: Harnessing genebanks: High-throughput phenotyping and genotyping of crop wild relatives and landraces

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Март 10, 2023

EDITORIAL article Front. Plant Sci., 10 March 2023Sec. Bioinformatics Volume 14 - 2023 | https://doi.org/10.3389/fpls.2023.1149469

Язык: Английский

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