International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(9), С. 3972 - 3972
Опубликована: Апрель 23, 2025
A
novel
primer
(FaSt-R)
targeting
the
Prunus-specific
Falling
Stone
(FaSt)
non-autonomous
transposon
was
combined
with
start-codon-targeted
(SCoT)
primers
to
assess
genetic
diversity
in
12
cultivars
from
six
Prunus
species
and
28
of
European
plum.
Compared
SCoT-only
analyses,
SCoT–FaSt
combination
produced
fewer
total
bands
but
a
higher
percentage
polymorphic
bands,
while
maintaining
comparable
values
for
polymorphism
information
content,
resolving
power,
gene
diversity,
Shannon’s
index.
markers
generated
across
broader
size
range,
which
made
gel
patterns
less
dense,
enabling
more
accurate
detection
differentially
amplified
fragments.
Neighbor-joining
principal
component
analyses
confirmed
that
provided
sufficient
phylogenetic
resolution
at
both
interspecific
intraspecific
levels.
The
sequencing
32
amplicons
revealed
FaSt
elements
26
fragments,
SCoT
preferentially
annealing
GC-rich
exonic
intergenic
regions.
Seventeen
protein-coding
one
RNA-coding
were
partially
identified,
localized
UTRs
introns
genes
key
physiological
functions.
Comparative
analysis
indicated
biased
distribution
between
Cerasus
subgenera.
In
silico
findings
suggest
are
fragmented
cherry
species,
potentially
contributing
subgeneric
divergence.
Overall,
marker
system
is
effective
evaluating
reconstructing
relationships,
elucidating
genomic
impact
an
active
Mutator-like
transposon.
Horticulturae,
Год журнала:
2023,
Номер
9(8), С. 902 - 902
Опубликована: Авг. 8, 2023
Micropropagation
has
an
important
role
in
the
large-scale
production
of
blackberry
plant
material,
given
high
proliferation
rates
this
species.
The
aim
present
study
was
to
evaluate
proliferative
capacity
grown
vitro
on
wheat
starch-gelled
culture
medium
compared
classical
agar-gelled
and
assess
genetic
fidelity
between
proliferated
shoots
their
mother
plants.
Six
varieties
(‘Čačanska
Bestrna’,
‘Chester
Thornless’,
‘Driscoll’s
Victoria’,
‘Loch
Ness’,
‘Polar’,
‘Karaka
Black’)
were
tested.
For
proliferation,
Murashige
Skoog
(MS)
supplemented
with
0.5
mg
dm−3
6-benzyladenine
(BA)
used.
conventional
gelled
0.5%
agar,
starch
used
as
alternative
gelling
agent
a
concentration
5%.
results
showed
that
for
all
cultivars,
highest
number
shoots/inoculum
obtained
medium,
maximum
value
54.42
±
4.18
presented
by
Black’.
Considering
length
shoots,
tested
cultivars
outstanding
5%
starch.
values
regarding
observed
Thornless’
followed
‘Čačanska
Ness’
5.55
0.04
cm,
5.46
0.06
5.37
0.09
respectively.
uniformity
micropropagated
relation
plants
confirmed
sequence-related
amplified
polymorphism
(SRAP)
start
codon
targeted
(SCoT)
molecular
markers.
Horticulturae,
Год журнала:
2024,
Номер
10(10), С. 1027 - 1027
Опубликована: Сен. 27, 2024
Juglans
regia
L.
is
an
edible
fruit
tree
cultivated
worldwide
for
its
fruits
and
wood
as
urban
tree.
Globally,
there
growing
concern
preserving
the
genetic
diversity
of
trees
with
high
economic
ecological
value.
This
study
investigates
J.
in
landscapes
assesses
quality
walnut
kernels
a
local
food
product.
An
inventory
150
from
five
populations
public
green
spaces
Lugoj,
Caransebeș
Jupa,
well
two
semi-natural
hilly
ecosystems
Banat
Region,
was
conducted.
Molecular
analyses
showed
that
Directed
Amplification
Minisatellite-region
DNA
(DAMD)
markers
were
more
effective
than
Start
Codon
Targeted
(SCoT)
markers,
higher
average
polymorphism
56.26%,
compared
to
49.44%.
DAMD07
achieved
100%
DAMD05
strong
balance
between
P.I.C.
(0.35)
(54.54%).
Chemical
analysis
revealed
following
contents
kernels:
protein
(12.81%
16.80%),
lipids
(60.39%
69.08%),
total
polyphenols
(5484.66
10,788.4
mg
GAE/kg),
copper
(3.655
8.532
mg/kg),
manganese
(14.408
28.618
zinc
(19.813
46.583
lead
(1.204
2.27
mg/kg)
cadmium
(0.03451
0.08065
mg/kg).
These
findings
are
critical
conservation
efforts,
forestry
management
ensuring
safety
products
derived
regia.
PeerJ,
Год журнала:
2025,
Номер
13, С. e18814 - e18814
Опубликована: Янв. 6, 2025
Pot
marigold
is
an
ornamental
plant
of
great
importance
in
pharmacy
and
cosmetology.
However,
there
limited
published
information
on
the
diversity
species
at
both
morphological
genetic
levels.
This
paper
aimed
to
determine
selected
varieties.
The
research
focused
varieties
with
different
flower
colours,
most
important
features
flowers
height
were
analysed.
Genetic
analyses
performed
using
start
codon
target
(SCoT)
markers.
correlation
coefficients
between
analysed
studied
showed
a
significant
positive
relationship
height,
number
inflorescences
per
inflorescence
diameter.
grouped
according
colour
their
flowers.
results
obtained
our
work
indicate
that,
despite
variability
observed
features,
gene
pool
limited.
can
be
valuable
for
breeders,
particularly
selecting
suitable
crossbreeding
breeding
programs.
Additionally,
it
offers
insights
into
resources
highlights
bioconservation.
Adaptive Fodder Production,
Год журнала:
2025,
Номер
2024(4), С. 18 - 26
Опубликована: Фев. 12, 2025
The
analysis
was
conducted
to
assess
the
inter-varietal
genetic
polymorphism
of
Poa
pratensis
based
on
PCR
technology
using
nine
SCoT
markers.
informative
primers
for
variety
differentiation
have
been
identified;
parameters
diversity
were
estimated:
Shannon’s
index,
Nei’s
effective
number
alleles
and
indicator
information
content
(PIC
—
content).
Genetic
structure
analyzed
collection
evaluated
Neighbor-Joining
dendrogram
PCoA-analysis.
As
a
result
research,
molecular
formulas
developed
that
can
be
used
as
individual
characteristic
originality
DNA
certification.
