Horticulturae,
Journal Year:
2023,
Volume and Issue:
9(8), P. 902 - 902
Published: Aug. 8, 2023
Micropropagation
has
an
important
role
in
the
large-scale
production
of
blackberry
plant
material,
given
high
proliferation
rates
this
species.
The
aim
present
study
was
to
evaluate
proliferative
capacity
grown
vitro
on
wheat
starch-gelled
culture
medium
compared
classical
agar-gelled
and
assess
genetic
fidelity
between
proliferated
shoots
their
mother
plants.
Six
varieties
(‘Čačanska
Bestrna’,
‘Chester
Thornless’,
‘Driscoll’s
Victoria’,
‘Loch
Ness’,
‘Polar’,
‘Karaka
Black’)
were
tested.
For
proliferation,
Murashige
Skoog
(MS)
supplemented
with
0.5
mg
dm−3
6-benzyladenine
(BA)
used.
conventional
gelled
0.5%
agar,
starch
used
as
alternative
gelling
agent
a
concentration
5%.
results
showed
that
for
all
cultivars,
highest
number
shoots/inoculum
obtained
medium,
maximum
value
54.42
±
4.18
presented
by
Black’.
Considering
length
shoots,
tested
cultivars
outstanding
5%
starch.
values
regarding
observed
Thornless’
followed
‘Čačanska
Ness’
5.55
0.04
cm,
5.46
0.06
5.37
0.09
respectively.
uniformity
micropropagated
relation
plants
confirmed
sequence-related
amplified
polymorphism
(SRAP)
start
codon
targeted
(SCoT)
molecular
markers.
Adaptive Fodder Production,
Journal Year:
2025,
Volume and Issue:
2024(4), P. 18 - 26
Published: Feb. 12, 2025
The
analysis
was
conducted
to
assess
the
inter-varietal
genetic
polymorphism
of
Poa
pratensis
based
on
PCR
technology
using
nine
SCoT
markers.
informative
primers
for
variety
differentiation
have
been
identified;
parameters
diversity
were
estimated:
Shannon’s
index,
Nei’s
effective
number
alleles
and
indicator
information
content
(PIC
—
content).
Genetic
structure
analyzed
collection
evaluated
Neighbor-Joining
dendrogram
PCoA-analysis.
As
a
result
research,
molecular
formulas
developed
that
can
be
used
as
individual
characteristic
originality
DNA
certification.
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Feb. 18, 2025
Abstract
The
three
highly
invasive
Reynoutria
species
–
R.
japonica
Houtt.,
sachalinensis
(F.Schmidt)
Nakai,
and
a
hybrid
of
them
x
bohemica
J.
Chrtek
&
A.
Chrtková
are
rich
sources
biologically
active
compounds.
However,
only
is
accepted
as
an
herbal
drug
source
in
the
pharmacopeias
Polygoni
cuspidati
rhizoma
et
radix
,
while
used
folk
phytotherapy.
can
be
misidentified
due
to
its
morphological
resemblance.
This
study
intended
explore
genetic
identity,
diversity,
population
structure
using
sequence-related
amplified
polymorphisms
(SRAP)
start
codon
targeted
(SCoT)
markers
correlated
with
characteristics
metabolic
profiles
fifteen
accessions
from
populations
Central
Europe
(Wroclaw,
south-west
Poland).
results
suggest
that
identification
based
on
leaf
morphology
clear
for
but
fails
distinguish
between
R
.
×
unambiguously.
qualitative
HPLC/DAD/ESI-HR-QTOF-MS
analysis
determined
chemical
composition
collected
samples,
revealing
117
compounds
belonging
carbohydrates,
stilbenes,
flavan-3-ols,
procyanidins,
anthraquinones,
organic
acids,
naphthalenes.
Out
these,
six
were
quantitated.
Based
LC-MS
data
compounds,
cluster
categorized
into
two
distinctive
clusters.
All
SCoT
SRAP
showed
medium
(0.1
0.25)
high
(0.30
0.40)
levels
PIC,
polymorphic
bands
(85
89%,
respectively)
relatively
mean
values
Rp
(6.42
7.0)
considered
informative
differentiating.
Partitioning
diversity
AMOVA
variation
within
was
higher
than
among
them,
seen
combined
(53.04%
vs.
46.96%,
analyses
individual
markers.
Genetic
indices
revealed
chosen
efficiently
assess
variability
genus
Na
varied
1.57
3.34
Ne
low
almost
identical
all
(~
1.6).
Nei’s
(H)
indicated
(<
0.2)
species,
highest
value
(0.184).
Despite
H
values,
Shannon’s
index
(I)
remains
similar
(
−
5.21,
5.28,
5.45).
number
(NPL)
percentage
loci
(PPL)
(160
52.98%)
twice
parent
species.
exhibited
moderate
G
ST
(0.159)
gene
flow
(Nm
=
1.317).
Evanno
test
(ΔK
2)
reveals
groups
admixture
population.
dendrograms
data,
distance,
clusters,
unequivocal
separation
both
other
taxa.
According
Baker’s
methodology,
correlation
observed
0.85.
Lower
correlations
noted
molecular
phytochemical
profile,
well
profile.
suggests
effectiveness
morphological,
phytochemical,
marker
systems
assessing
identity
plant
material.
approach
also
further
studies
structure,
spread,
evolution
such
spp.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(9), P. 3972 - 3972
Published: April 23, 2025
A
novel
primer
(FaSt-R)
targeting
the
Prunus-specific
Falling
Stone
(FaSt)
non-autonomous
transposon
was
combined
with
start-codon-targeted
(SCoT)
primers
to
assess
genetic
diversity
in
12
cultivars
from
six
Prunus
species
and
28
of
European
plum.
Compared
SCoT-only
analyses,
SCoT–FaSt
combination
produced
fewer
total
bands
but
a
higher
percentage
polymorphic
bands,
while
maintaining
comparable
values
for
polymorphism
information
content,
resolving
power,
gene
diversity,
Shannon’s
index.
markers
generated
across
broader
size
range,
which
made
gel
patterns
less
dense,
enabling
more
accurate
detection
differentially
amplified
fragments.
Neighbor-joining
principal
component
analyses
confirmed
that
provided
sufficient
phylogenetic
resolution
at
both
interspecific
intraspecific
levels.
The
sequencing
32
amplicons
revealed
FaSt
elements
26
fragments,
SCoT
preferentially
annealing
GC-rich
exonic
intergenic
regions.
Seventeen
protein-coding
one
RNA-coding
were
partially
identified,
localized
UTRs
introns
genes
key
physiological
functions.
Comparative
analysis
indicated
biased
distribution
between
Cerasus
subgenera.
In
silico
findings
suggest
are
fragmented
cherry
species,
potentially
contributing
subgeneric
divergence.
Overall,
marker
system
is
effective
evaluating
reconstructing
relationships,
elucidating
genomic
impact
an
active
Mutator-like
transposon.
