Metabolites,
Год журнала:
2024,
Номер
14(2), С. 112 - 112
Опубликована: Фев. 7, 2024
Specialized
metabolites
are
produced
via
discrete
metabolic
pathways.
These
small
molecules
play
significant
roles
in
plant
growth
and
development,
as
well
defense
against
environmental
stresses.
include
damping
off
or
seedling
blight
at
a
post-emergence
stage.
Targeted
metabolomics
was
followed
to
gain
insights
into
metabolome
changes
characteristic
of
different
developmental
stages
sorghum
seedlings.
Metabolites
were
extracted
from
leaves
seven
time
points
post-germination
analyzed
using
ultra-high
performance
liquid
chromatography
coupled
mass
spectrometry.
Multivariate
statistical
analysis
combined
with
chemometric
tools,
such
principal
component
analysis,
hierarchical
clustering
orthogonal
partial
least
squares–discriminant
applied
for
data
exploration
reduce
dimensionality
the
selection
potential
discriminant
biomarkers.
Changes
patterns
seedlings
early,
middle,
late
(7,
14,
29
days
post-germination).
The
metabolite
classes
amino
acids,
organic
lipids,
cyanogenic
glycosides,
hormones,
hydroxycinnamic
acid
derivatives,
flavonoids,
latter
representing
largest
class
metabolites.
In
general,
content
showed
an
increase
progression
stages.
Most
differential
derived
tryptophan
phenylalanine,
which
contribute
innate
immune
defenses
growth.
Quantitative
identified
correlation
apigenin
flavone
derivatives
Data-driven
investigations
these
metabolomes
provided
new
dynamics
that
occur
limit
mortality.
Plants,
Год журнала:
2025,
Номер
14(7), С. 989 - 989
Опубликована: Март 21, 2025
Drought
stress
significantly
impairs
the
output
of
tea
plants
and
quality
products.
Although
Serendipita
indica
has
demonstrated
ability
to
enhance
drought
tolerance
in
host
plants,
its
impact
on
(Camellia
sinensis)
experiencing
is
unknown.
This
study
assessed
response
by
inoculating
S.
under
conditions.
Phenotypic
physiological
analyses
that
mitigated
damage
regulating
osmotic
equilibrium
antioxidant
enzyme
activity.
Metabolome
analysis
showed
promoted
accumulation
flavonoid
metabolites,
including
naringin,
(-)-epiafzelechin,
naringenin
chalcone,
dihydromyricetin,
while
inhibiting
content
amino
acids
derivatives,
such
as
homoarginine,
L-arginine,
N6-acetyl-L-lysine,
N-palmitoylglycine,
during
water
deficit.
The
expression
patterns
indica-stimulated
genes
were
investigated
using
transcriptome
analysis.
indica-induced
drought-responsive
involved
regulation,
protection,
transcription
factors,
signaling
identified
recognized
possibly
significant
indica-mediated
plants.
Particularly,
biosynthesis
pathway
was
from
metabolomic
transcriptomic
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
enrichment
Moreover,
biosynthesis-related
identified.
indica-inoculation
upregulated
cinnamate
4-hydroxylase
(C4H),
chalcone
synthase
(CHS),
flavanone
3-hydroxylase
(F3H),
dihydroflavonol
4-reductase
(DFR),
anthocyanidin
reductase
(ANR),
leucoanthocyanidin
(LAR)
compared
uninoculated
subjected
stress.
Consequently,
we
concluded
inoculation
primarily
alleviates
modulating
pathway.
These
results
will
provide
insights
into
mechanisms
indica-enhanced
establish
a
solid
foundation
for
application
microbial
agent
management
cultivation.
Agronomy,
Год журнала:
2023,
Номер
13(12), С. 3006 - 3006
Опубликована: Дек. 7, 2023
The
aim
of
the
present
study
is
to
evaluate
absolute
content
and
accumulation
patterns
flavonoid
components;
give
insight
into
relationships
among
explore
correlation
between
components
expression
biosynthesis
genes
in
Tartary
buckwheat
seeds;
construct
a
biosynthetic
pathway
on
major
seeds.
In
total,
61
were
absolutely
quantified
five
varieties,
which
41
existed
all
varieties.
most
flavonoids
varied
significantly
different
varieties
or
within
same
variety.
Rutin,
quercetin,
nicotiflorin,
kaempferol
dominant
seeds,
accounting
for
73.05–81.79%
total
flavonoids.
Significantly
positive
negative
correlations
with
found
some
components.
Thirty-six
displayed
four
developing
Seventeen
structural
during
development
F3′5′H-3
gene
might
be
crucial
contributor
determining
A
schematic
pathways
30
seeds
was
constructed.
These
findings
provide
an
outlook
their
have
potential
applications
breeding
new
cultivars
higher
contents.
Frontiers in Plant Science,
Год журнала:
2024,
Номер
15
Опубликована: Апрель 16, 2024
Honeysuckle,
valued
for
its
wide-ranging
uses
in
medicine,
cuisine,
and
aesthetics,
faces
a
significant
challenge
cultivation
due
to
powdery
mildew,
primarily
caused
by
the
Erysiphe
lonicerae
pathogen.
The
interaction
between
honeysuckle
E.
,
especially
concerning
disease
progression,
remains
insufficiently
understood.
Our
study,
conducted
three
different
locations,
found
that
naturally
infected
with
showed
notable
decreases
total
flavonoid
content,
reductions
of
34.7%,
53.5%,
53.8%
observed
each
respective
site.
Controlled
experiments
supported
these
findings,
indicating
artificial
inoculation
led
20.9%
reduction
levels
over
21
days,
worsening
54.8%
decrease
day
42.
Additionally,
there
was
drop
plant’s
antioxidant
capacity,
reaching
an
81.7%
56
days
after
inoculation.
Metabolomic
analysis
also
revealed
substantial
essential
medicinal
components
such
as
chlorogenic
acid,
luteolin,
quercetin,
isoquercetin,
rutin.
Investigating
gene
expression
marked
relative
LjPAL1
gene,
starting
early
7
post-inoculation
falling
minimal
level
(fold
change
=
0.29)
35.
This
trend
mirrored
consistent
phenylalanine
ammonia-lyase
activity
through
entire
process,
which
decreased
72.3%
56.
Further
sustained
repression
downstream
genes
LjFNHO1
LjFNGT1
closely
linked
.
We
identified
mechanism
inhibits
this
pathway
suggest
may
strategically
weaken
honeysuckle’s
resistance
targeting
key
biosynthetic
pathways,
thereby
facilitating
further
pathogen
invasion.
Based
on
our
we
recommend
two
primary
strategies:
first,
monitoring
constituent
from
-affected
areas
ensure
therapeutic
effectiveness;
second,
emphasizing
prevention
control
measures
against
mildew
persistent
decline
crucial
active
compounds.
Journal of Advanced Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 1, 2025
Acetic
acid
(HAC)
is
a
crucial
signal
molecule
in
plant
stress
responses;
however,
its
role
conferring
heat
tolerance
to
rice
remains
unclear.
This
study
aims
investigate
the
effect
of
HAC
protecting
pistil
function
under
and
potential
facilitating
pollen
germination
tube
growth
via
HAC-induced
synthesis
methyl
jasmonate
(MeJA)
quercetin
(QR).
Physiological
analysis,
including
germination,
into
ovule,
reactive
oxygen
species
(ROS),
as
well
levels
HAC,
acetyl
coenzyme
A
(acetyl-CoA),
MeJA,
QR
pistils
stress-treated
early
indica
cultivars
Zhongzao39
(ZZ39)
Zhongjiazao17
(ZJZ17),
were
conducted.
RNA
sequencing
(RNA-seq)
was
performed
identify
differentially
expressed
genes
involved
this
process.
Effect
exogenous
acetate
(NaAC),
on
spikelet
fertility
also
investigated.
Compared
with
ZJZ17,
severe
inhibition
fertility,
observed
ZZ39,
due
ROS
burst
an
irregular
distribution
across
stigma,
style,
ovule.
RNA-seq
physiological
data
indicate
that
may
activate
acetyl-CoA
enhance
by
inducing
MeJA
QR.
Exogenous
NaAC
enhanced
stress,
accompanied
elevated
antioxidant
enzyme
activities,
improved
energy
status,
increased
acetyl-CoA,
pistils.
Additionally,
NaAC,
QR,
either
alone
or
combination,
effectively
augmented
while
combination
inhibitors
significantly
reduced
fertility.
Acetate
activates
induce
both
thereby
alleviating
heat-induced
dysfunction
maintaining
homeostasis
enhancing
Our
results
offer
promising
strategy
crops.
