Food Bioscience, Год журнала: 2024, Номер 63, С. 105682 - 105682
Опубликована: Дек. 11, 2024
Язык: Английский
Plant Physiology and Biochemistry, Год журнала: 2024, Номер 208, С. 108503 - 108503
Опубликована: Март 1, 2024
Язык: Английский
Процитировано
15
Separations, Год журнала: 2024, Номер 11(5), С. 145 - 145
Опубликована: Май 8, 2024
In this study, eleven kinds of flavonoids were identified from F. tataricum bran (FTB) by UPLC-Q-TOF-MS, and HPLC-DAD analysis revealed that four compounds, including rutin, quercetin, kaempferol, nicotiflorin, the most significant components. Subsequently, natural deep eutectic solvent-based ultrasound-assisted extraction (NADES-UAE) was employed to extract FTB. Among six NADES prepared, choline chloride–ethylene glycol (ChCl-EG) as a promising candidate for extracting due its superior performance. The conditions statistically investigated using response surface methodology conducted Box-Behnken design (BBD). optimal operational follows: ultrasonic time 268 s, temperature 76 °C, liquid–solid ratio 43 mL/g, which resulted in high total flavonoid yield 40.29 mg/g. Afterwards, efficient mechanism NADES-UAE comprehensively explored through FT-IR spectra, COSMO model, microstructural analysis. conclusion, is considered green, efficient, sustainable method FTB flavonoids.
Язык: Английский
Процитировано
6
Chemistry & Biodiversity, Год журнала: 2025, Номер unknown
Опубликована: Янв. 7, 2025
Boraginaceae plants, including four endemic species from Türkiye, were analyzed for organic and inorganic compositions using inductively coupled plasma mass spectrometry (ICP-MS) liquid chromatography-mass spectrometry/mass (LC-MS/MS) to explore their nutritional, medicinal, ecological significance. This study examined 18 species, identifying key elements, such as sodium (87 600.359-118 049.272 g/kg), potassium (98 876.885-145 587.899 iron (70 396.436-116 416.076 which showed significant variation. Metabolite profiling revealed the presence of alkaloids, flavonoids, tannins in most samples. Additionally, 42 amino acids 35 phenolic compounds detected, with rosmarinic acid proline being particularly abundant. Rosmarinic was concentrated Oi Ml samples, whereas levels ranged 3023.8086 7693.8549 nmol/mL. The highlights intricate metabolic elemental profiles shedding light on adaptations therapeutic potentials. Spearman correlation analysis suggested relationships among compounds, acids, compositions, indicating potential applications nutrition, pharmacology, biodiversity conservation.
Язык: Английский
Процитировано
0
Plants, Год журнала: 2025, Номер 14(2), С. 203 - 203
Опубликована: Янв. 13, 2025
Tartary buckwheat is a nutrient-rich pseudo-cereal whose starch contents, including amylose and amylopectin their properties hold significant importance for enhancing yield quality. The granule-bound synthase (GBSS) key enzyme responsible the synthesis of amylose, directly determining content amylose-to-amylopectin ratio in crops. Although one has already been cloned, GBSS genes at genome-wide level have not yet fully assessed thoroughly analyzed buckwheat. This study comprehensively FtGBSSs Based on genome data buckwheat, five FtGBSS genes, namely FtGBSS-1 to FtGBSS-5, were identified three chromosomes, exhibiting about 1800 bp lengths CDSs numerous exons introns gene structures. Amino acid analyses revealed high homology ten proteins from rice, maize, Arabidopsis thaliana, with specific catalytic domain conserved motifs. had closer relationship monocot based evolutionary analysis. Expression suggested that showed distinct tissue-specific expression patterns rice-Tartary Among them, FtGBSS-1, FtGBSS-2, FtGBSS-4 higher expressed root, stem, or flower, suggesting they role these tissues. Notably, FtGBSS-3 FtGBSS-5 more highly seeds than other tissues, pivotal Furthermore, cis acting elements promoters binding transcription factors (TFs) investigated. A protein–protein interaction network was constructed co-expression FtGBSSs, TFs, belonging bZIP, ERF, bHLH, MADS-box TF families, within this network, significantly correlated two seed-specific (FtGBSS-3 FtGBSS-5). Finally, FtGBSS1-5 successfully transformed into rice through transgenic manipulation, overexpression lines an increase accompanied by reduction total contents compared WT. Overall, research only deepens our understanding molecular mechanisms but also provides scientific insights crop quality breeding.
Язык: Английский
Процитировано
0
Plant Physiology and Biochemistry, Год журнала: 2025, Номер 223, С. 109804 - 109804
Опубликована: Март 20, 2025
Язык: Английский
Процитировано
0
Journal of Cereal Science, Год журнала: 2025, Номер unknown, С. 104180 - 104180
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Food Chemistry Advances, Год журнала: 2024, Номер 5, С. 100820 - 100820
Опубликована: Сен. 24, 2024
Язык: Английский
Процитировано
3
Agronomy, Год журнала: 2024, Номер 14(8), С. 1826 - 1826
Опубликована: Авг. 19, 2024
According to the requirements of Tartary buckwheat breeding, it is necessary develop a method for rapid detection functional substances in seeds. To ensure diverse sample pool, we utilized stable recombinant inbred lines (RILs) buckwheat. The coefficients variation total flavonoid, vitamin E (VE), and GABA contents RIL population were 15.06, 16.53, 36.93, respectively. Subsequently, established prediction models substance using near-infrared spectroscopy (NIRS) combined with chemometrics. Kennard–Stone algorithm divided dataset into training test sets, employing six different methods preprocessing spectra. Competitive Adaptive Reweighted Sampling extracted characteristic best flavonoid VE normalized first derivative. calibration correlation coefficient (Rc) (Rp) greater than 0.94. optimal model underwent via normalization standard normal variate, Rc Rp values 0.93. results demonstrated that NIRS-based could satisfy determination flavonoids, VE,
Язык: Английский
Процитировано
1
Plants, Год журнала: 2024, Номер 13(19), С. 2704 - 2704
Опубликована: Сен. 27, 2024
Tartary buckwheat (Fagopyrum tataricum Gaertn.) is a coarse grain crop rich in flavonoids that are beneficial to human health because they function as anti-inflammatories and provide protection against cardiovascular disease diabetes. Flavonoid biosynthesis complex process, relatively little known about the regulatory pathways involved buckwheat. Here, we cloned characterized FtMYB163 gene from buckwheat, which encodes member of R2R3-MYB transcription factor family. Amino acid sequence phylogenetic analysis indicate subgroup 7 (SG7) closely related FeMYBF1, regulates flavonol synthesis common (F. esculentum). We demonstrated localizes nucleus has transcriptional activity. Expression levels roots, stems, leaves, flowers, seeds F. were positively correlated with total flavonoid contents these tissues. Overexpression transgenic Arabidopsis enhanced expression several genes early (AtCHS, AtCHI, AtF3H, AtFLS) significantly increased accumulation flavonoids, including naringenin chalcone, naringenin-7-O-glucoside, eriodictyol, eight compounds. Our findings demonstrate by changing key biosynthetic pathways.
Язык: Английский
Процитировано
1
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0