Application of CRISPR/Cas Technology in Biological Nano‐Analysis DOI
Pingping Liu,

Gerile Aodeng,

Lu Ga

и другие.

ChemistrySelect, Год журнала: 2023, Номер 8(45)

Опубликована: Дек. 5, 2023

Abstract Currently, with the growing global demand for infectious disease diagnostics, environmental testing and food safety, CRISPR‐Cas (Clustered Regularly Interspaced Short Palindromic Repeats‐CRISPR associated proteins) system has unlimited potential as one of emerging technologies in field biotechnology. Recent studies have shown that become a powerful weapon gene editing, treatment, molecular diagnosis imaging. As important biomarkers, various nucleic acid non‐nucleic targets need to be detected from samples before situation deteriorates. In addition, there are still some limitations challenges current biosensor platforms based on system. Therefore, summarising strategies, strengths weaknesses, development prospects target identification is beneficial fully explore directions CRISPR‐Cas‐based systems. This review briefly introduces summarises classification, mechanisms applications (diagnostics when combined signal detection means). limitations, future optimisation this discussed.

Язык: Английский

Nano-Food Farming: Toward Sustainable Applications of Proteins, Mushrooms, Nano-Nutrients, and Nanofibers DOI Creative Commons
József Prokisch, Gréta Törős, Duyen H. H. Nguyen

и другие.

Agronomy, Год журнала: 2024, Номер 14(3), С. 606 - 606

Опубликована: Март 18, 2024

The relationship between agriculture and food is very close. It impossible to produce adequate crops for global security without proper farm management. Farming practices represent direct indirect controlling factors in terms of security. management influence agro-food production from seed germination through the post-harvest treatments. Nano-farming utilizes nanotechnologies agricultural production. This review covers four key components nano-farming: nano-mushroom production, protein-based nanoparticles, nano-nutrients, nanofibers. provides a comprehensive overview potential applications nanotechnology agriculture. role these will be discussed relation challenges faced solutions required achieve sustainable Edible mushrooms are important because they nutritious source can nanoparticles that used other sources. Protein-based have considerable delivery bioactives as carriers applications. Nano-nutrients (mainly nano-selenium, nano-tellurium carbon nanodots) crucial impacts on nutrient status plant-based foods. Carbon nanodots carbon-based nanomaterials positively. There promising nanofibers packaging, safety processing. However, further research needed understand risks system.

Язык: Английский

Процитировано

3

Navigating the Path from Lab to Market: Regulatory Challenges and Opportunities for Genome Editing Technologies for Agriculture DOI
Mayla Daiane Corrêa Molinari, Renata Fuganti‐Pagliarini, Lílian H. Florentino

и другие.

Опубликована: Янв. 1, 2024

Over recent decades, an array of molecular tools has been applied in plant genome engineering, including TALENs (transcription activator-like effector nucleases), ZFNs (zinc-finger and CRISPR/Cas systems (clustered regularly interspaced short palindromic repeats). At present, have caught significant industry attention owing to their cost-effectiveness precision genomic modulation, thereby serving as a potent tool science research. Importantly, plants subjected editing via might not be classified genetically modified organisms (GMO), which could streamline acceptance worldwide. Originally discovered defense mechanism against plasmids invading viruses bacteria archaea, the system includes two components: CRISPR ribonucleic acid (crRNA) Cas protein. The crRNA guides protein specific (DNA) target sequence. Once there, cleaves sequence, impeding replication. In relation editing, researchers distinct sequences, manipulated function either endonuclease or base editor. most frequently used enzymes from Type II are CRISPR/Cas9 CRISPR/Cas12a (Cpf1). other harbor immense potential revolutionize breeding biotechnology. Nevertheless, use must undergo stringent regulation ensure safe responsible application. future holds promise for with safety being paramount concern crop gene editing. As such, it is vital perpetuate research development this field fully exploit its advantages agriculture because, technology advances new emerge, becomes crucial governments keep abreast cutting-edge scientific progress. This awareness allows balance between benefits associated ethical considerations.

Язык: Английский

Процитировано

3

A Split-Marker System for CRISPR-Cas9 Genome Editing in Methylotrophic Yeasts DOI Open Access
Azamat V. Karginov,

М. Г. Тарутина,

A. R. Lapteva

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(9), С. 8173 - 8173

Опубликована: Май 3, 2023

Methylotrophic yeasts such as Ogataea polymorpha and Komagataella phaffii (sin. Hansenula Pichia pastoris, respectively) are commonly used in basic research biotechnological applications, frequently those requiring genome modifications. However, the CRISPR-Cas9 editing approaches reported for these species so far relatively complex laborious. In this work we present an improved plasmid vector set methylotrophic yeasts. This includes a encoding Cas9 with nuclear localization signal plasmids scaffold single guide RNA (sgRNA). Construction of sgRNA gene particular target sequence requires only insertion 24 bp oligonucleotide duplex into scaffold. Prior to yeast transformation, each is cleaved at two sites, one which located within selectable marker, that functional marker can be restored via recombination Cas9-containing fragment gene-containing fragment. leads formation autonomously replicating plasmid, lost from clones after acquisition required modification. The allows use G418-resistance LEU2 auxotrophic markers. functionality setup has been demonstrated O. polymorpha, parapolymorpha, haglerorum phaffii.

Язык: Английский

Процитировано

4

Method for growing edibleEuglena gracilisin an inexpensive medium with tomato juice to a high cell density equivalent to the density in KH medium DOI Creative Commons
Kyôhei Yamashita, Koji Yamada, Kengo Suzuki

и другие.

Sustainable Food Technology, Год журнала: 2023, Номер 1(5), С. 709 - 721

Опубликована: Янв. 1, 2023

This paper proposes a low-cost technology for growing Euglena gracilis using beverages that people consume on daily basis as nutrient source.

Язык: Английский

Процитировано

4

Advancement of animal and poultry nutrition: Harnessing the power of CRISPR-Cas genome editing technology DOI Creative Commons
Mahbuba Akther Mishu, Sabuj Kanti Nath, M. Sohidullah

и другие.

Journal of Advanced Veterinary and Animal Research, Год журнала: 2024, Номер 11(2), С. 483 - 483

Опубликована: Янв. 1, 2024

CRISPR-associated proteins and clustered regularly interspaced short palindromic repeats (CRISPR-Cas) technology has emerged as a groundbreaking advancement in animal poultry nutrition to improve feed conversion efficiency, enhance disease resistance, the nutritional quality of products. Despite significant advancements, there is research gap systematic understanding comprehensive use CRISPR-Cas method nutrition. The purpose this study elucidate latest advancements through genome editing technology, focusing on gene manipulation metabolism, immunity, growth. Following preferred reporting items meta-analysis reviews guidelines, we conducted search using several databases, including Scopus, PubMed, Web Science, until May 2024, finally, included total 108 articles study. This article explores system additives like probiotics enzymes, which could reduce antibiotics production. Furthermore, discusses ethical regulatory issues related nutrition, concerns about welfare, food safety, environmental impacts. Overall, holds substantial promise overcome challenges modern agriculture. By enriching products, increasing improving it offers sustainable cost-effective solutions that can revolutionize

Язык: Английский

Процитировано

1

Biotechnology executive order opens door for regulatory reform and social acceptance of genetically engineered microbes in agriculture DOI Creative Commons

Jabeen Ahmad,

Amy M. Grunden, Jennifer Kuzma

и другие.

GM crops & food, Год журнала: 2024, Номер 15(1), С. 248 - 261

Опубликована: Июль 27, 2024

In the United States, regulatory review of genetically engineered microbes for agriculture falls under Coordinated Framework Regulation Biotechnology (CFRB). However, lack a centralized pathway and multiple oversight authorities can lead to uncertainty in review. Using three microbial-based technologies as illustrative examples, this commentary identifies weaknesses challenges associated with CFRB by assessing current system proposed changes multi criteria decision analysis framework. addition, it discusses opportunities reform improve clarity, efficiency, public acceptance CHIPS Science Act 2022 Executive Order on Bioeconomy.

Язык: Английский

Процитировано

1

SOCIETY 5.0 AND ITS IMPACT ON AGRICULTURAL BUSINESS AND INNOVATION: A NEW PARADIGM FOR RURAL DEVELOPMENT DOI Creative Commons
Cariša Bešić, Dragan Ćoćkalo, Mihalj Bakator

и другие.

Ekonomika poljoprivrede, Год журнала: 2024, Номер 71(3), С. 803 - 819

Опубликована: Окт. 4, 2024

This paper analyzes the impact of Society 5.0 on agricultural business and innovation, proposing a new paradigm for rural development. represents evolution beyond previous societal models, aiming to harmonize economic progress with solutions social issues through integration cyberspace physical space. Central this model is application advanced technologies such as Internet Things (IoT), artificial intelligence (AI), robotics, big data, augmented reality. The study focuses significant changes within practices models. Through review analysis current trends, presents theoretical framework also proposes Agricultural Business Rural Development Potential (ABRDP) index guide future trends potential outcomes in domain, offering insights into optimistic, conservative, pessimistic scenarios

Язык: Английский

Процитировано

1

CRISPR-Cas and Its Applications in Food Production DOI
Nitish Rai, Namita Ashish Singh,

J. K. Goyal

и другие.

Опубликована: Янв. 1, 2024

The increasing global population and decline in the availability of new arable land water resources, along with environmental change, have created many challenges for food industry. As per estimates, nearly 8% world may get affected by malnutrition starvation 2030. faced industry require effective production crops vegetables enhanced nutritional values a higher shelf life. Traditional methods including breeding hybridization are now being replaced genetically modified crops. Advanced such as CRISPR-Cas derived from bacterial immune system been widely used genome editing creation varieties their straightforward design, high efficiency, good reproducibility, quick cycle. CRISPR differs classical GM crop technique, which inserts exogenous or foreign DNA vector into plant genome; instead, it can be to remove any undesired gene using its precise mechanism cut DNA; therefore, produced this method more ethically acceptable. In chapter, we described basic aspects related biology, genomic organization, mechanism, merits. Furthermore, application technology yield quality enhancing resistance against viruses, bacteria, fungi, bioactive compound rice, wheat, soybeans, fruits, potato tomato is also discussed. safety regulations pertaining use CRISPR-modified products highlighted discussing several regions across globe.

Язык: Английский

Процитировано

0

CRISPR/Cas Systems for Enhancing Photosynthesis: Climate Resilience and Food Production DOI

Meena Barupal,

Ashwani Kumar

Опубликована: Янв. 1, 2024

To achieve the Sustainable Development Goal 2 (SDG 2) established by UN, there is a need to produce 15–20% more food from recent trends of production. Biotechnological advances in late twentieth century paved path for creation genetically engineered crop species enhance agricultural Recently programmable gene editing tools such as zinc-finger nucleases (ZFN), transcription activator-like effector (TALE) nucleases, clustered regularly interspaced short palindromic repeat (CRISPR) systems, and base editors have been developed edit any genome desirable traits. Furthermore, plant organelle genomes are distinct, encoding numerous genes crucial photosynthesis respiration. Any alteration photosynthetic functionality can directly impact Photosynthesis enzymes evolutionarily conserved green plants. There huge scope CRISPR genomics transform routine crops into superfoods. Chloroplast-targeting with magic cut allows engineering higher expression useful traits increased specific types oil content seeds or inclusion all essential vitamins plant-based diets. Knock-out knock-in genes, especially those related photosystem small subunits key CO2-fixing enzyme Rubisco (SSU-encoded rbcS) disease—resistance other intermediate metabolites plants facilitate high productivity even under climate change scenario. CRISPR-Cas9 enables multiple polyploid which remained challenging long time. Supercharging C3 advancement process production calories limited land area fertilizer. In this review, we address CRISPR-Cas9-based uses, challenges improve targeted efficiency

Язык: Английский

Процитировано

0

Biotechnological advances in plant growth-promoting rhizobacteria for sustainable agriculture DOI
Leandris Argentel‐Martínez, Ofelda Peñuelas‐Rubio, Angélica Herrera‐Sepúlveda

и другие.

World Journal of Microbiology and Biotechnology, Год журнала: 2024, Номер 41(1)

Опубликована: Дек. 30, 2024

Язык: Английский

Процитировано

0