Abstract
Currently,
with
the
growing
global
demand
for
infectious
disease
diagnostics,
environmental
testing
and
food
safety,
CRISPR‐Cas
(Clustered
Regularly
Interspaced
Short
Palindromic
Repeats‐CRISPR
associated
proteins)
system
has
unlimited
potential
as
one
of
emerging
technologies
in
field
biotechnology.
Recent
studies
have
shown
that
become
a
powerful
weapon
gene
editing,
treatment,
molecular
diagnosis
imaging.
As
important
biomarkers,
various
nucleic
acid
non‐nucleic
targets
need
to
be
detected
from
samples
before
situation
deteriorates.
In
addition,
there
are
still
some
limitations
challenges
current
biosensor
platforms
based
on
system.
Therefore,
summarising
strategies,
strengths
weaknesses,
development
prospects
target
identification
is
beneficial
fully
explore
directions
CRISPR‐Cas‐based
systems.
This
review
briefly
introduces
summarises
classification,
mechanisms
applications
(diagnostics
when
combined
signal
detection
means).
limitations,
future
optimisation
this
discussed.
Agronomy,
Год журнала:
2024,
Номер
14(3), С. 606 - 606
Опубликована: Март 18, 2024
The
relationship
between
agriculture
and
food
is
very
close.
It
impossible
to
produce
adequate
crops
for
global
security
without
proper
farm
management.
Farming
practices
represent
direct
indirect
controlling
factors
in
terms
of
security.
management
influence
agro-food
production
from
seed
germination
through
the
post-harvest
treatments.
Nano-farming
utilizes
nanotechnologies
agricultural
production.
This
review
covers
four
key
components
nano-farming:
nano-mushroom
production,
protein-based
nanoparticles,
nano-nutrients,
nanofibers.
provides
a
comprehensive
overview
potential
applications
nanotechnology
agriculture.
role
these
will
be
discussed
relation
challenges
faced
solutions
required
achieve
sustainable
Edible
mushrooms
are
important
because
they
nutritious
source
can
nanoparticles
that
used
other
sources.
Protein-based
have
considerable
delivery
bioactives
as
carriers
applications.
Nano-nutrients
(mainly
nano-selenium,
nano-tellurium
carbon
nanodots)
crucial
impacts
on
nutrient
status
plant-based
foods.
Carbon
nanodots
carbon-based
nanomaterials
positively.
There
promising
nanofibers
packaging,
safety
processing.
However,
further
research
needed
understand
risks
system.
Over
recent
decades,
an
array
of
molecular
tools
has
been
applied
in
plant
genome
engineering,
including
TALENs
(transcription
activator-like
effector
nucleases),
ZFNs
(zinc-finger
and
CRISPR/Cas
systems
(clustered
regularly
interspaced
short
palindromic
repeats).
At
present,
have
caught
significant
industry
attention
owing
to
their
cost-effectiveness
precision
genomic
modulation,
thereby
serving
as
a
potent
tool
science
research.
Importantly,
plants
subjected
editing
via
might
not
be
classified
genetically
modified
organisms
(GMO),
which
could
streamline
acceptance
worldwide.
Originally
discovered
defense
mechanism
against
plasmids
invading
viruses
bacteria
archaea,
the
system
includes
two
components:
CRISPR
ribonucleic
acid
(crRNA)
Cas
protein.
The
crRNA
guides
protein
specific
(DNA)
target
sequence.
Once
there,
cleaves
sequence,
impeding
replication.
In
relation
editing,
researchers
distinct
sequences,
manipulated
function
either
endonuclease
or
base
editor.
most
frequently
used
enzymes
from
Type
II
are
CRISPR/Cas9
CRISPR/Cas12a
(Cpf1).
other
harbor
immense
potential
revolutionize
breeding
biotechnology.
Nevertheless,
use
must
undergo
stringent
regulation
ensure
safe
responsible
application.
future
holds
promise
for
with
safety
being
paramount
concern
crop
gene
editing.
As
such,
it
is
vital
perpetuate
research
development
this
field
fully
exploit
its
advantages
agriculture
because,
technology
advances
new
emerge,
becomes
crucial
governments
keep
abreast
cutting-edge
scientific
progress.
This
awareness
allows
balance
between
benefits
associated
ethical
considerations.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(9), С. 8173 - 8173
Опубликована: Май 3, 2023
Methylotrophic
yeasts
such
as
Ogataea
polymorpha
and
Komagataella
phaffii
(sin.
Hansenula
Pichia
pastoris,
respectively)
are
commonly
used
in
basic
research
biotechnological
applications,
frequently
those
requiring
genome
modifications.
However,
the
CRISPR-Cas9
editing
approaches
reported
for
these
species
so
far
relatively
complex
laborious.
In
this
work
we
present
an
improved
plasmid
vector
set
methylotrophic
yeasts.
This
includes
a
encoding
Cas9
with
nuclear
localization
signal
plasmids
scaffold
single
guide
RNA
(sgRNA).
Construction
of
sgRNA
gene
particular
target
sequence
requires
only
insertion
24
bp
oligonucleotide
duplex
into
scaffold.
Prior
to
yeast
transformation,
each
is
cleaved
at
two
sites,
one
which
located
within
selectable
marker,
that
functional
marker
can
be
restored
via
recombination
Cas9-containing
fragment
gene-containing
fragment.
leads
formation
autonomously
replicating
plasmid,
lost
from
clones
after
acquisition
required
modification.
The
allows
use
G418-resistance
LEU2
auxotrophic
markers.
functionality
setup
has
been
demonstrated
O.
polymorpha,
parapolymorpha,
haglerorum
phaffii.
Journal of Advanced Veterinary and Animal Research,
Год журнала:
2024,
Номер
11(2), С. 483 - 483
Опубликована: Янв. 1, 2024
CRISPR-associated
proteins
and
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR-Cas)
technology
has
emerged
as
a
groundbreaking
advancement
in
animal
poultry
nutrition
to
improve
feed
conversion
efficiency,
enhance
disease
resistance,
the
nutritional
quality
of
products.
Despite
significant
advancements,
there
is
research
gap
systematic
understanding
comprehensive
use
CRISPR-Cas
method
nutrition.
The
purpose
this
study
elucidate
latest
advancements
through
genome
editing
technology,
focusing
on
gene
manipulation
metabolism,
immunity,
growth.
Following
preferred
reporting
items
meta-analysis
reviews
guidelines,
we
conducted
search
using
several
databases,
including
Scopus,
PubMed,
Web
Science,
until
May
2024,
finally,
included
total
108
articles
study.
This
article
explores
system
additives
like
probiotics
enzymes,
which
could
reduce
antibiotics
production.
Furthermore,
discusses
ethical
regulatory
issues
related
nutrition,
concerns
about
welfare,
food
safety,
environmental
impacts.
Overall,
holds
substantial
promise
overcome
challenges
modern
agriculture.
By
enriching
products,
increasing
improving
it
offers
sustainable
cost-effective
solutions
that
can
revolutionize
GM crops & food,
Год журнала:
2024,
Номер
15(1), С. 248 - 261
Опубликована: Июль 27, 2024
In
the
United
States,
regulatory
review
of
genetically
engineered
microbes
for
agriculture
falls
under
Coordinated
Framework
Regulation
Biotechnology
(CFRB).
However,
lack
a
centralized
pathway
and
multiple
oversight
authorities
can
lead
to
uncertainty
in
review.
Using
three
microbial-based
technologies
as
illustrative
examples,
this
commentary
identifies
weaknesses
challenges
associated
with
CFRB
by
assessing
current
system
proposed
changes
multi
criteria
decision
analysis
framework.
addition,
it
discusses
opportunities
reform
improve
clarity,
efficiency,
public
acceptance
CHIPS
Science
Act
2022
Executive
Order
on
Bioeconomy.
Ekonomika poljoprivrede,
Год журнала:
2024,
Номер
71(3), С. 803 - 819
Опубликована: Окт. 4, 2024
This
paper
analyzes
the
impact
of
Society
5.0
on
agricultural
business
and
innovation,
proposing
a
new
paradigm
for
rural
development.
represents
evolution
beyond
previous
societal
models,
aiming
to
harmonize
economic
progress
with
solutions
social
issues
through
integration
cyberspace
physical
space.
Central
this
model
is
application
advanced
technologies
such
as
Internet
Things
(IoT),
artificial
intelligence
(AI),
robotics,
big
data,
augmented
reality.
The
study
focuses
significant
changes
within
practices
models.
Through
review
analysis
current
trends,
presents
theoretical
framework
also
proposes
Agricultural
Business
Rural
Development
Potential
(ABRDP)
index
guide
future
trends
potential
outcomes
in
domain,
offering
insights
into
optimistic,
conservative,
pessimistic
scenarios
The
increasing
global
population
and
decline
in
the
availability
of
new
arable
land
water
resources,
along
with
environmental
change,
have
created
many
challenges
for
food
industry.
As
per
estimates,
nearly
8%
world
may
get
affected
by
malnutrition
starvation
2030.
faced
industry
require
effective
production
crops
vegetables
enhanced
nutritional
values
a
higher
shelf
life.
Traditional
methods
including
breeding
hybridization
are
now
being
replaced
genetically
modified
crops.
Advanced
such
as
CRISPR-Cas
derived
from
bacterial
immune
system
been
widely
used
genome
editing
creation
varieties
their
straightforward
design,
high
efficiency,
good
reproducibility,
quick
cycle.
CRISPR
differs
classical
GM
crop
technique,
which
inserts
exogenous
or
foreign
DNA
vector
into
plant
genome;
instead,
it
can
be
to
remove
any
undesired
gene
using
its
precise
mechanism
cut
DNA;
therefore,
produced
this
method
more
ethically
acceptable.
In
chapter,
we
described
basic
aspects
related
biology,
genomic
organization,
mechanism,
merits.
Furthermore,
application
technology
yield
quality
enhancing
resistance
against
viruses,
bacteria,
fungi,
bioactive
compound
rice,
wheat,
soybeans,
fruits,
potato
tomato
is
also
discussed.
safety
regulations
pertaining
use
CRISPR-modified
products
highlighted
discussing
several
regions
across
globe.
To
achieve
the
Sustainable
Development
Goal
2
(SDG
2)
established
by
UN,
there
is
a
need
to
produce
15–20%
more
food
from
recent
trends
of
production.
Biotechnological
advances
in
late
twentieth
century
paved
path
for
creation
genetically
engineered
crop
species
enhance
agricultural
Recently
programmable
gene
editing
tools
such
as
zinc-finger
nucleases
(ZFN),
transcription
activator-like
effector
(TALE)
nucleases,
clustered
regularly
interspaced
short
palindromic
repeat
(CRISPR)
systems,
and
base
editors
have
been
developed
edit
any
genome
desirable
traits.
Furthermore,
plant
organelle
genomes
are
distinct,
encoding
numerous
genes
crucial
photosynthesis
respiration.
Any
alteration
photosynthetic
functionality
can
directly
impact
Photosynthesis
enzymes
evolutionarily
conserved
green
plants.
There
huge
scope
CRISPR
genomics
transform
routine
crops
into
superfoods.
Chloroplast-targeting
with
magic
cut
allows
engineering
higher
expression
useful
traits
increased
specific
types
oil
content
seeds
or
inclusion
all
essential
vitamins
plant-based
diets.
Knock-out
knock-in
genes,
especially
those
related
photosystem
small
subunits
key
CO2-fixing
enzyme
Rubisco
(SSU-encoded
rbcS)
disease—resistance
other
intermediate
metabolites
plants
facilitate
high
productivity
even
under
climate
change
scenario.
CRISPR-Cas9
enables
multiple
polyploid
which
remained
challenging
long
time.
Supercharging
C3
advancement
process
production
calories
limited
land
area
fertilizer.
In
this
review,
we
address
CRISPR-Cas9-based
uses,
challenges
improve
targeted
efficiency