Biotechnology & Biotechnological Equipment,
Год журнала:
2022,
Номер
36(1), С. 838 - 847
Опубликована: Дек. 31, 2022
Confronting
the
global
spread
of
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2),
simple,
fast
and
specific
non-laboratory
SARS-CoV-2
diagnostic
tests
are
urgently
required.
However,
current
nucleic
acid
assays
generally
rely
on
laboratory,
trained
staff
specialized
equipment
for
execution
analysis,
presenting
clear
limitations
in
field
detection.
Here,
we
describe
a
portable
reliable
immobilization-based
loop-mediated
isothermal
amplification
(LAMP)
device
which
is
mobile,
without
requirement
any
complicated
instrument
appropriate
high-throughput
testing.
This
was
constructed
by
utilizing
interaction
between
carboxyl-tagged
primer
an
amino-tagged
substrate,
capable
catching
target
sequence
produced
via
LAMP.
In
this
study,
immobilization
conditions
immobilized
structure
were
explored
optimized.
With
proposed
device,
analysis
result
can
be
obtained
rapidly
30
min
with
excellent
specificity,
even
if
template
extracted
from
complex
sample
containing
pharyngeal
swab
or
human
blood.
addition,
applied
to
detect
various
other
pathogens,
showing
attractive
potential
rapid
detection
at
airports,
railway
stations,
cold-chain
transportations,
community
hospitals
so
on.
Therefore,
believe
that
LAMP
advanced
approach
developing
portable,
specific,
low-cost
platform.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(34), С. 12710 - 12718
Опубликована: Авг. 18, 2023
We
report
the
development
of
a
reproducible
and
highly
sensitive
surface-enhanced
Raman
scattering
(SERS)
substrate
using
butanol-induced
self-assembly
gold
nanoparticles
(AuNPs)
its
application
as
rapid
diagnostic
platform
for
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2).
The
process
was
used
to
generate
uniform
assembly
AuNPs,
with
multiple
hotspots,
achieve
high
reproducibility.
When
an
aqueous
droplet
containing
AuNPs
target
DNAs
dropped
onto
butanol
droplet,
dehydration
occurred,
enriching
around
increasing
loading
density
on
AuNP
surface.
SERS
evaluated
by
spectroscopy,
which
showed
strong
electromagnetic
enhancement
signals.
then
tested
detection
SARS-CoV-2
SERS,
very
low
limit
(LoD)
3.1
×
10-15
M
obtained.
This
provides
sufficient
sensitivity
screening
assay,
time
is
significantly
reduced
no
thermocycling
steps
are
required.
study
demonstrates
method
SARS-CoV-2.
results
suggest
potential
this
approach
developing
platforms
other
biomolecules
infectious
diseases.
Archives of Microbiology,
Год журнала:
2023,
Номер
205(6)
Опубликована: Май 17, 2023
Abstract
COVID-19
is
a
highly
infectious
disease
caused
by
the
SARS-CoV-2
virus,
which
primarily
affects
respiratory
system
and
can
lead
to
severe
illness.
The
virus
extremely
contagious,
early
accurate
diagnosis
of
crucial
contain
its
spread,
provide
prompt
treatment,
prevent
complications.
Currently,
reverse
transcriptase
polymerase
chain
reaction
(RT-PCR)
considered
be
gold
standard
for
detecting
in
stages.
In
addition,
loop-mediated
isothermal
amplification
(LMAP),
clustering
rule
interval
short
palindromic
repeats
(CRISPR),
colloidal
immunochromatographic
assay
(GICA),
computed
tomography
(CT),
electrochemical
sensors
are
also
common
tests.
However,
these
different
methods
vary
greatly
terms
their
detection
efficiency,
specificity,
accuracy,
sensitivity,
cost,
throughput.
Besides,
most
current
conducted
central
hospitals
laboratories,
great
challenge
remote
underdeveloped
areas.
Therefore,
it
essential
review
advantages
disadvantages
methods,
as
well
technology
that
enhance
efficiency
improve
quality
greater
details.
The Analyst,
Год журнала:
2023,
Номер
148(15), С. 3622 - 3631
Опубликована: Янв. 1, 2023
Herein,
we
introduce
a
novel
assay
for
multiple-gene
recognition
by
ligation-double
transcription
mediated
fluorometric
profiling.
We
demonstrated
the
capability
of
system
to
identify
potential
multi-gene
classifiers
diagnostic
use
employing
combination
approach
with
selective
fluorophore
probe-RNA
hybridization/graphene
oxide
quenching
system.
The
is
efficient
and
requires
only
45
minutes
total
experimentation
offers
high
sensitivity
(369.6,
408,
407.8
copies
per
mL
O,
E,
N
genes
SARS-CoV-2,
respectively)
specificity
(selective
until
two
mismatches).
Our
expected
expedite
precise
diagnosis
RNA-virus-related
diseases
multiple
gene
classifiers.
By
focusing
on
distinct
viral
genes,
our
method
allowed
detection
various
RNA
viruses
in
variety
sample
pools.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(48), С. 17613 - 17621
Опубликована: Ноя. 18, 2023
Photosensitizers
and
photothermal
agents
have
attracted
increasing
attention
for
in
vitro
diagnosis,
but
the
combination
remains
challenging.
Herein,
a
light-driven
photocatalytic–photothermal
synergetic
system
integrated
microfluidic
distance-based
analytical
device
(PCPT-μDAD)
visual,
portable,
sensitive,
quantitative
detection
of
targets
was
developed.
Target
DNA
recognized
initiated
hybridization
chain
reaction
to
form
double-stranded
DNA/SYBR
Green
I
(dsDNA/SG-I)
complex.
By
applying
photosensitization
dsDNA/SG-I
complex
effect
oxidized
3,3′,5,5′-tetramethylbenzidine,
target
concentration
can
effectively
translate
into
visual
distance
signal
readout.
Importantly,
PCPT-μDAD
greatly
improves
controllability
catalytic
reactions
amplification
efficiency.
The
shows
low
limit
(fM
level),
good
stability,
high
reproducibility
nucleic
acid
detection.
The Analyst,
Год журнала:
2024,
Номер
149(17), С. 4514 - 4524
Опубликована: Янв. 1, 2024
In
this
study,
we
developed
an
isothermal
fluorometric
diagnostic
method
for
DNA
virus-generating
disorders
such
as
Mpox.
Our
results
showed
that
the
release
of
a
large
number
protons
during
multiplex-LAMP
markedly
lowered
pH
level,
which
transformed
retinoblastoma
(Rb)
linear
ssDNA
into
i-motifs.
Consequently,
thiazole
orange
(TO;
probe
sensitive
to
i-motif)
boosted
signal-on
fluorescence
because
its
ability
bind
selectively
This
multiplex-LAMP/i-motif-TO
system
enabled
simultaneous
detection
aimed
at
numerous
potential
targets
with
remarkable
sensitivity
(1.47
pg
per
mL)
and
efficiency
(30
minutes).
is
expected
enable
DNA-virus-related
diseases
be
efficiently
accurately
assessed.
