Revealing the gastric protective mechanism of Physochlaina physaloides (L) G. Don. with clinical orientation: PPAR/NF-κB signalling pathway activation
Phytomedicine,
Год журнала:
2025,
Номер
141, С. 156642 - 156642
Опубликована: Март 16, 2025
Gastrointestinal
diseases
are
global
health
issues.
Current
drugs
for
gastrointestinal
cause
discomfort
and
toxicity;
consequently,
the
use
of
traditional
medicines
their
extracts
has
gained
attention
in
recent
years.
Physochlaina
physaloides
(L)
G.
Don.
(P.
physaloides)
is
traditionally
used
diarrhoea
gastroenteritis;
however,
its
material
basis
mechanism
action
gastric
injury
have
not
been
fully
studied.
This
study
aims
to
explore
P.
protective
effects
on
injury,
together
with
potential
mechanisms.
We
constructed
chronic
gastritis
ulcer
models
rats
using
56
%
ethanol
anhydrous
ethanol,
respectively.
Additionally,
we
screened
pathways
via
transcriptomics
gene
expression
omnibus
(GEO)
database.
Subsequently,
an
ethanol-stimulated
GES-1
cell
model
active
fraction
based
survival
rate
antioxidant
activity.
The
effect
was
investigated
tissue
structure
(HE
staining),
mucus
secretion
(PAS
anti-inflammatory
activity,
acid
levels.
employed
liquid
chromatography-tandem
mass
spectrometry
(LC-MS/MS)
determine
components
drug
blood,
before
investigating
mechanisms
immunofluorescence,
transcriptomic,
metabolomics,
network
pharmacology,
molecular
docking,
qRT-PCR,
western
blotting,
flow
cytometry.
occurrence
gastritis,
ulcer,
cancer
related
PPAR/NF-κB
signalling
pathway,
decreased
FABP3
PPARγ,
increased
Bcl-2
TNF-α.
n-butanol
(BPP)
showed
significant
improvement
activity
vitro.
BPP
also
alleviated
inflammation
oxidative
stress
rat
models,
including
by
upregulating
CAT,
GSH,
SOD,
IL-10,
PGE2;
downregulating
VEGFA,
IL-6,
IL-8,
TNF-α,
NO;
improving
pathological
damage;
restoring
levels;
reducing
macrophage
expression.
components,
anisodamine
hyoscyamine,
upregulated
PPARα,
CPT1,
FABP3,
downregulated
NF-κB
p65,
thereby
regulating
pathway
gastroprotection.
did
significantly
increase
GPX4
SLC7A11,
nor
they
reduce
production
ROS.
Therefore,
unrelated
ferroptosis.
provides
first
evidence
effectiveness
prevention
ulcers
treatment
gastritis.
adopted
a
multidisciplinary
approach
demonstrate
that
protect
against
ethanol-induced
non-ferroptotic
death
pathways.
can
target
PPARγ
may
clinical
application
prospects
prevent
which
unique
advantages.
These
findings
provide
scientific
foundation
gastroprotection
expand
applications
BPP.
Язык: Английский
The potential of metal–organic framework MIL-101(Al)–NH2 in the forefront of antiviral protection of cells via interaction with SARS-CoV-2 spike RBD protein and their antibacterial action mediated with hypericin and photodynamic treatment
Journal of Colloid and Interface Science,
Год журнала:
2025,
Номер
unknown, С. 137454 - 137454
Опубликована: Март 1, 2025
Язык: Английский