DNAzyme@MOF breaking pH limitation for the detection of dopamine in the interstitial fluid

Biosensors and Bioelectronics, Год журнала: 2025, Номер unknown, С. 117367 - 117367

Опубликована: Март 1, 2025

Язык: Английский

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Recent progress and challenges in ECL biosensors for the identification of small extracellular vesicle and clinical diagnosis

TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118253 - 118253

Опубликована: Апрель 1, 2025

Язык: Английский

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Multilayered MXene electrodes in deep eutectic solvent ionic liquid electrolyte for supercapacitor applications

Journal of Alloys and Compounds, Год журнала: 2025, Номер unknown, С. 179442 - 179442

Опубликована: Фев. 1, 2025

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Closed-loop cascade nanozyme strategy for mutually reinforced catalytic and mild-temperature photothermal therapeutic effects

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Апрель 4, 2025

Nanocatalysis coupled with photothermal therapy is a potent anti-cancer approach, yet its clinical utility limited by low concentration of tumor substrate, redox interference, and risks overheating normal tissues. Herein, we propose an innovative closed-loop nanozyme approach that leverages the synergistic effects catalytic mild (mPTT) to address aforementioned challenges. The strategy features folic acid-functionalized iron single-atom catalyst (FeNC-FA), designed exhibit exceptional multienzymatic capabilities optimal response. In system, engineered FeNC-FA capable inducing reactive oxygen species (ROS) storm depleting glutathione (GSH) in specific microenvironment (TME) initiate ferroptosis. Concurrently, accumulation ROS effectively cleaves heat shock proteins (HSPs), thereby enhancing mPTT. An intriguing aspect increased temperature within TME further facilitates conversion H2O2 O2, alleviating hypoxia providing positive feedback circuit boost therapy. Additionally, advanced photoacoustic (PA) imaging allow for self-monitoring their at sites, guiding mPTT process. Taken together, it provides PA image-guided, mutually reinforced both vitro vivo. This targeted holds great promise personalized medicine applications.

Язык: Английский

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Porphyrinic metal-organic framework PCN-224 supported Prussian blue enables selective detection of casein and phytic acid

Talanta, Год журнала: 2025, Номер unknown, С. 128121 - 128121

Опубликована: Апрель 1, 2025

Язык: Английский

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MOF-engineered Cu2O nanozymes with boosted peroxidase-like activity for colorimetric-fluorescent dual-mode detection of deoxynivalenol

Microchimica Acta, Год журнала: 2025, Номер 192(5)

Опубликована: Апрель 24, 2025

Язык: Английский

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Highly Sensitive and Diversified Electrochemiluminescence DNA Methylation Biosensing Platform Based on Self-Assembly of Nanotags

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 29, 2025

This work proposes a highly sensitive, simple, and reliable electrochemiluminescence (ECL) DNA methylation biosensing platform by employing DNA-functionalized magnetic beads (DNA-MBs) for target capture nanotag self-assembly aggregation signal amplification. The methylated was first captured on DNA-MBs through base pairing recognition, then its sites were recognized antibody-5mC (Ab-5mC). Afterward, pair of antibodies functionalized [Ru(byp)3]2+-doped silica nanoparticles (Ab2-Ru@SiO2 Ab3-Ru@SiO2) layer-by-layer assembled Ab-5mC amplified transduction. sensing could be transferred to screen-printed carbon electrodes (SPCEs) ECL curve detection via photomultiplier tube or gold-coated indium tin oxide (Au/ITO) arrays high-throughput imaging detection. As the assembly layers increased from 1 3, sensitivities SPCE-based Au/ITO-based enhanced 7-fold 3-fold, achieving limits down 0.8 pM 0.9 fM, respectively. nanotags showed good stability, with storage times 300 days Ru@SiO2 60 Ab-Ru@SiO2, method is universal applied detect different DNAs using their corresponding DNA-MBs. proposed possessed advantages high sensitivity, diversity, practicality, showing potential in clinical diagnosis.

Язык: Английский

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Functional 2D MXene-DNA Hybrid Hydrogel for Portable Detection of Blood Disorder Biomarker Thrombin in Human Plasma

Journal of Materials Chemistry B, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

A schematic representation of the workflow thrombin detection system. The addition a sample containing to MXene–DNA hydrogel leads specific resistance after applying it Pt-printed button circuit.

Язык: Английский

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Development of Suspended Droplet Microextraction Method for Spectrophotometric Determination of Serum Iron

Analytical Science Advances, Год журнала: 2025, Номер 6(1)

Опубликована: Май 14, 2025

ABSTRACT A facile, selective and sensitive method was devised for the Fe 3+ quantification in low volume samples, such as bovine serum based on suspended droplet microextraction (SDME). Various process parameters concentrations of acid (1.5% hydrochloric acid), complexing agent (0.7% ammonium thiocyanate), quaternary salt (0.2% Aliquat 336) extracting solvent (500 µL octanol) were optimised. Ammonium thiocyanate forms water soluble, red coloured, anionic ferric complex [Fe(SCN) 6 ] 3− with ions released from iron–protein under an acidic medium. Negatively charged hydrophobic ion associate Fe(SCN) –Aliquat 336 3 hydrophilic NH 4 + head groups drives out formed micelle aqueous solution along iron complex. After stirring, bonded micelles are separated into a hanging micro octanol. Red coloured is solubilised methanol concentration samples obtained by recording spectrophotometric absorbance at 505 nm. The recoveries ranged 96.2%–98.8% relative standard deviation (RSD) (%) values 1.4% to 5.0% 100–400 ng/mL confirming interference free optimised conditions. developed linear over range 20–1000 limit detection 2.4 matrix. applied various 62.7 1582.5 ng/mL. accordance results electrothermal atomic absorption spectrometry 99% confidence level using t ‐test indicating accuracy method. proposed procedure offers advantages enhanced sensitivity spectrophotometer towards determination, low‐cost agent, sample volume, metal biological free, simplicity selectivity. Thus, can be alternative routine analysis other fluids.

Язык: Английский

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An Efficient Luminol–H₂O₂ Electrochemiluminescence System with Porous Bimetallic Organic Gels as Signal Booster and Elaborate Heterosequence Aptamer as Recognition Component for Ultrasensitive Biosensing

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 15, 2025

Herein, an efficient luminol-H2O2 electrochemiluminescence (ECL) system with bimetallic organic gels as ECL signal booster and innovative heterosequence aptamer recognition a target conversion strategy is used to construct sensitive specific aptasensor for the detection of β2-microglobulin (B2M), key biomarker end-stage renal disease (ESRD). Impressively, porous Fe@Cu (Fe@Cu MOGs) act coreaction accelerators confinement-enhanced reactors system, amplifying by 21-fold compared traditional which greatly enhanced sensitivity biosensor. Compared homosequence approach competitive binding aptamers single site, synergistic multiple sites could improve specificity aptasensor, validated experiments molecular docking simulations. Therefore, developed exhibits remarkable dynamic range 10 fg/mL-1 μg/mL ultralow limit 0.9 fg/mL, superior previously reported works. Additionally, demonstrated consistent performance conventional clinical immunoturbidimetric assays high B2M concentration in 14 samples, well exhibiting trace levels that are undetectable immunoturbidimetry. This offers accurate platform recognition, promising applications detection, diagnosis, therapeutic monitoring, advancing scientific research on early pathological changes discovery.

Язык: Английский

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